Rh5CG321400

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008176
Physical Location & Seq
Forward (+)
35561509 .. 35574443
12935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG321400.1

Sequence Viewer

Length: 945 bp
ATGGCTGCATTCCAGACATGCTTACAAGTCTGGTTGACATCATCCCTTCTTGCTGATGGACTAGCTGTTGCAGGACAGGACAGAAACTGCCCAATTGATCTGAAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATGGATATCCGCAAGCACTTCACAGCAAAATACGGAAAGGAGTTCGTCTCTGCAACTGTTGAACTACGGTCGGATAGTGGTGTAAGCCGCATGTTAATAGAGAAATTATCTACCAAATCACCTGATGGCTCTACCAAGATGAAAATTTTGGCTACAATTGCAGAGGAACATAATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTCCTGAGGACCTGCTGAACGGACCAAATACGTTTGGAATGGCCAGTATGGATTCTGCTCCACCTCCACCTACTCATGATGTCAGGGGACCTCCTAATGTCCAAGTTCCGCCTAATAGCATGGCCAGCAAAGCTCATGTGGAACCTCCCTCTGGTCCACCTCCACCTGATCAACCTCCAATTGCTCAAGTTCCACGCAAAAATTATGAGTATCACGATGGTCCTATGAATTCTAATGAGCAGAATGCAAGATCATCACGTCACTTGGAGGATTCTGCTTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTGCCAAATTTCATCCAGAAACGACATCTTCAGGTACATCAATTTGTCACATAAAGATTGTGACCCCAACTCAACCACAGAAGCTGCCGTTGCCTCTGCTTCCACCACCTGCAGCGTGCCATTTGCTTCGGTATCCATCAGTTCCTCCACAGGAGGTTCCTCATCATCAAGCTCCAGAGCAGTATATATTTGGATTTTATATTCTCTGCCCAGATCAATCACTCGCCGTCTCAACTATTTGTGAGTATGTCCATTTCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

314

Amino Acids

34.21

Weight (kDa)

5.83

Isoelectric Point (pI)

60.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 114 2.1e-28 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 799
Acc36I ACCTGC 2 cut(s) 390, 799
AciI CCGC 4 cut(s) 146, 172, 250, 479
AclWI GGATC 2 cut(s) 341, 354
AcoI YGGCCR 2 cut(s) 411, 492
AcsI RAATTY 3 cut(s) 306, 598, 689
AcuI CTGAAG 2 cut(s) 122, 696
AfaI GTAC 2 cut(s) 153, 718
AfiI CCNNNNNNNGG 1 cut(s) 521
AgsI TTSAA 2 cut(s) 224, 362
AjiI CACGTC 1 cut(s) 629
AluBI AGCT 4 cut(s) 65, 503, 766, 854
AluI AGCT 4 cut(s) 65, 503, 766, 854
Alw26I GTCTC 2 cut(s) 214, 916
AlwI GGATC 2 cut(s) 341, 354
AlwNI CAGNNNCTG 2 cut(s) 87, 766
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 2 cut(s) 411, 492
ApeKI GCWGC 3 cut(s) 5, 766, 794
ApoI RAATTY 3 cut(s) 306, 598, 689
AspS9I GGNCC 5 cut(s) 379, 392, 458, 524, 590
AsuHPI GGTGA 1 cut(s) 273
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 5 cut(s) 379, 392, 458, 524, 590
AxyI CCTNAGG 1 cut(s) 375
BalI TGGCCA 2 cut(s) 413, 494
BamHI GGATCC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 753, 806
BccI CCATC 4 cut(s) 50, 281, 581, 826
BceAI ACGGC 2 cut(s) 754, 893
BciVI GTATCC 1 cut(s) 825
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 2 cut(s) 214, 916
BfaI CTAG 2 cut(s) 62, 138
BfmI CTRYAG 1 cut(s) 792
BfuAI ACCTGC 2 cut(s) 390, 799
BfuI GTATCC 1 cut(s) 825
BisI GCNGC 4 cut(s) 6, 250, 767, 795
BlsI GCNGC 4 cut(s) 7, 251, 768, 796
Bme18I GGWCC 5 cut(s) 379, 392, 458, 524, 590
BmeT110I CYCGRG 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 629
BmgT120I GGNCC 5 cut(s) 379, 392, 458, 524, 590
BmiI GGNNCC 4 cut(s) 348, 459, 513, 840
BmsI GCATC 1 cut(s) 140
BplI GAGNNNNNCTC 2 cut(s) 194, 226
BpmI CTGGAG 1 cut(s) 840
BpuEI CTTGAG 1 cut(s) 540
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse1I ACTGG 1 cut(s) 414
Bse21I CCTNAGG 1 cut(s) 375
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 2 cut(s) 41, 694
BseLI CCNNNNNNNGG 1 cut(s) 521
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 414
BseXI GCAGC 2 cut(s) 753, 806
Bsh1285I CGRYCG 1 cut(s) 233
BshFI GGCC 2 cut(s) 413, 494
BsiEI CGRYCG 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 350
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 521
BsmAI GTCTC 2 cut(s) 214, 916
BsmBI CGTCTC 2 cut(s) 214, 916
BsmFI GGGAC 1 cut(s) 471
BsmI GAATGC 2 cut(s) 8, 619
BsnI GGCC 2 cut(s) 413, 494
BsoBI CYCGRG 1 cut(s) 350
Bsp143I GATC 5 cut(s) 97, 346, 538, 620, 895
BspACI CCGC 4 cut(s) 146, 172, 250, 479
BspANI GGCC 2 cut(s) 413, 494
BspCNI CTCAG 1 cut(s) 367
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 4 cut(s) 348, 459, 513, 840
BspMAI CTGCAG 1 cut(s) 796
BspMI ACCTGC 2 cut(s) 390, 799
BspPI GGATC 2 cut(s) 341, 354
BsrI ACTGG 1 cut(s) 414
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 5 cut(s) 97, 346, 538, 620, 895
Bst4CI ACNGT 2 cut(s) 220, 231
Bst6I CTCTTC 1 cut(s) 357
BstC8I GCNNGC 3 cut(s) 176, 496, 799
BstDEI CTNAG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 41, 694
BstKTI GATC 5 cut(s) 100, 349, 541, 623, 898
BstMAI GTCTC 2 cut(s) 214, 916
BstMBI GATC 5 cut(s) 97, 346, 538, 620, 895
BstMCI CGRYCG 1 cut(s) 233
BstMWI GCNNNNNNNGC 5 cut(s) 320, 495, 500, 671, 772
BstNSI RCATGY 2 cut(s) 21, 256
BstSFI CTRYAG 1 cut(s) 792
BstV1I GCAGC 2 cut(s) 753, 806
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu36I CCTNAGG 1 cut(s) 375
BsuI GTATCC 1 cut(s) 825
BsuRI GGCC 2 cut(s) 413, 494
BtrI CACGTC 1 cut(s) 629
BtsCI GGATG 2 cut(s) 41, 694
BveI ACCTGC 2 cut(s) 390, 799
Cac8I GCNNGC 3 cut(s) 176, 496, 799
CaiI CAGNNNCTG 2 cut(s) 87, 766
CciI TCATGA 1 cut(s) 445
Cfr13I GGNCC 5 cut(s) 379, 392, 458, 524, 590
Csp6I GTAC 2 cut(s) 152, 717
CviAII CATG 6 cut(s) 18, 163, 253, 446, 490, 506
CviQI GTAC 2 cut(s) 152, 717
DdeI CTNAG 1 cut(s) 375
DpnI GATC 5 cut(s) 99, 348, 540, 622, 897
DpnII GATC 5 cut(s) 97, 346, 538, 620, 895
EaeI YGGCCR 2 cut(s) 411, 492
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
EciI GGCGGA 1 cut(s) 468
Eco32I GATATC 1 cut(s) 169
Eco47I GGWCC 5 cut(s) 379, 392, 458, 524, 590
Eco57I CTGAAG 2 cut(s) 122, 696
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 350
EcoO109I RGGNCCY 2 cut(s) 379, 458
EcoRI GAATTC 1 cut(s) 598
EcoRV GATATC 1 cut(s) 169
Esp3I CGTCTC 2 cut(s) 214, 916
FaeI CATG 6 cut(s) 21, 166, 256, 449, 493, 509
FaqI GGGAC 1 cut(s) 471
FatI CATG 6 cut(s) 17, 162, 252, 445, 489, 505
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 4 cut(s) 6, 250, 767, 795
FokI GGATG 2 cut(s) 28, 681
Fsp4HI GCNGC 4 cut(s) 6, 250, 767, 795
FspBI CTAG 2 cut(s) 62, 138
GluI GCNGC 4 cut(s) 6, 250, 767, 795
GsuI CTGGAG 1 cut(s) 840
HaeIII GGCC 2 cut(s) 413, 494
Hin1II CATG 6 cut(s) 21, 166, 256, 449, 493, 509
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 2 cut(s) 422, 641
HphI GGTGA 1 cut(s) 273
Hpy166II GTNNAC 2 cut(s) 36, 527
Hpy188I TCNGA 2 cut(s) 102, 235
Hpy188III TCNNGA 7 cut(s) 13, 350, 374, 446, 584, 698, 857
Hpy8I GTNNAC 2 cut(s) 36, 527
HpyAV CCTTC 2 cut(s) 56, 97
HpyCH4III ACNGT 2 cut(s) 220, 231
HpyCH4IV ACGT 3 cut(s) 150, 401, 628
HpyCH4V TGCA 7 cut(s) 8, 71, 131, 215, 323, 617, 794
HpyF10VI GCNNNNNNNGC 5 cut(s) 320, 495, 500, 671, 772
HpyF3I CTNAG 1 cut(s) 375
HpySE526I ACGT 3 cut(s) 150, 401, 628
Hsp92II CATG 6 cut(s) 21, 166, 256, 449, 493, 509
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 5 cut(s) 97, 346, 538, 620, 895
LmnI GCTCC 2 cut(s) 433, 859
Lsp1109I GCAGC 2 cut(s) 753, 806
LweI GCATC 1 cut(s) 140
MaeI CTAG 2 cut(s) 62, 138
MaeII ACGT 3 cut(s) 150, 401, 628
MaeIII GTNAC 3 cut(s) 629, 728, 742
MalI GATC 5 cut(s) 99, 348, 540, 622, 897
MboI GATC 5 cut(s) 97, 346, 538, 620, 895
MboII GAAGA 2 cut(s) 374, 702
MfeI CAATTG 3 cut(s) 93, 318, 549
MflI RGATCY 1 cut(s) 346
MlsI TGGCCA 2 cut(s) 413, 494
MluNI TGGCCA 2 cut(s) 413, 494
MmeI TCCRAC 1 cut(s) 213
Mox20I TGGCCA 2 cut(s) 413, 494
MscI TGGCCA 2 cut(s) 413, 494
MseI TTAA 1 cut(s) 257
Msp20I TGGCCA 2 cut(s) 413, 494
MunI CAATTG 3 cut(s) 93, 318, 549
Mva1269I GAATGC 2 cut(s) 8, 619
MwoI GCNNNNNNNGC 5 cut(s) 320, 495, 500, 671, 772
NdeII GATC 5 cut(s) 97, 346, 538, 620, 895
NlaIII CATG 6 cut(s) 21, 166, 256, 449, 493, 509
NlaIV GGNNCC 4 cut(s) 348, 459, 513, 840
NmuCI GTSAC 3 cut(s) 629, 728, 742
NspI RCATGY 2 cut(s) 21, 256
PagI TCATGA 1 cut(s) 445
PaqCI CACCTGC 1 cut(s) 799
PctI GAATGC 2 cut(s) 8, 619
PfeI GAWTC 2 cut(s) 422, 641
PkrI GCNGC 4 cut(s) 7, 251, 768, 796
PpuMI RGGWCCY 2 cut(s) 379, 458
Psp5II RGGWCCY 2 cut(s) 379, 458
PspN4I GGNNCC 4 cut(s) 348, 459, 513, 840
PspPI GGNCC 5 cut(s) 379, 392, 458, 524, 590
PspPPI RGGWCCY 2 cut(s) 379, 458
PstI CTGCAG 1 cut(s) 796
PstNI CAGNNNCTG 2 cut(s) 87, 766
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 2 cut(s) 153, 718
RsaNI GTAC 2 cut(s) 152, 717
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 4 cut(s) 6, 250, 767, 795
Sau3AI GATC 5 cut(s) 97, 346, 538, 620, 895
Sau96I GGNCC 5 cut(s) 379, 392, 458, 524, 590
SfaNI GCATC 1 cut(s) 140
SfcI CTRYAG 1 cut(s) 792
SinI GGWCC 5 cut(s) 379, 392, 458, 524, 590
SmlI CTYRAG 1 cut(s) 555
SmoI CTYRAG 1 cut(s) 555
SsiI CCGC 4 cut(s) 146, 172, 250, 479
SspMI CTAG 2 cut(s) 62, 138
TaaI ACNGT 2 cut(s) 220, 231
TaiI ACGT 3 cut(s) 153, 404, 631
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 2 cut(s) 422, 641
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TseFI GTSAC 3 cut(s) 629, 728, 742
TseI GCWGC 3 cut(s) 5, 766, 794
Tsp45I GTSAC 3 cut(s) 629, 728, 742
TspDTI ATGAA 3 cut(s) 317, 611, 683
TspGWI ACGGA 2 cut(s) 210, 405
VpaK11BI GGWCC 5 cut(s) 379, 392, 458, 524, 590
XapI RAATTY 3 cut(s) 306, 598, 689
XceI RCATGY 2 cut(s) 21, 256
XspI CTAG 2 cut(s) 62, 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.