Rh5DG249300

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008176
Physical Location & Seq
Reverse (-)
28735517 .. 28743695
8179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG249300.1

Sequence Viewer

Length: 915 bp
ATGGCCGCCAACCGCCCTGCCGTATCTTCTCCGCCGCCGCAGCGAAGAAGGACCATGTCCGCTCTCGAAGCCCGAATCTCCCTCATTGCCGCTCTCGCCACCCAAGCCTCCTCCATTGAAAATGAGTATGGACCCATGGAATACGAAATTGGTGCACCAGGTAAAGCTTACACTGACTGGATAGCTCAAATGGTTGTGGGATTAAACACTAGTTTCCCCTGGATCCTGTGCAAACAAGATGATGCCCCTGATCCTGTTGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATGGATATCCGCAAACACTTCACAGCAAAATATGGAAAAGAGTTCGTCTCTATAGCTGTTGAACTACGGCCGGATAGTGGTGTAAGCCGCATGTTAATAGAGAAATTATCTACCAAATCACCTAATGGCCCTACCAAGATGAAAATTTTGGCTGCAATTGCAGAGGAACATAATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTCCTGAGGACCTGCTGAACAGACCAAATACGTTTGGAATGGCCAGTATGGATTCTGCTCAACCTCCACCTACTCATGATGTCAGGGGACCTCCTAATGTCCAAGTTCCGCCTAATAGCATGGCCAGTAAAGCTCATGTACGTCCACCTGATCAACCTCCAATTGCTCAAGTTCCACGCAGAAATTATGAGTATCACGATGGACCTATGAATTCTAAAGAGCAGAATGCAAGATCATCTCGTCACTCTGATGATTCTGCTTCTACAAATGTTTCTGCAAACAAAGCAACCACATCTGCCAAATTTCATCCAGAAATGACATCTTCAGGTACATCAATTTCTCACATAAAGATTGTGGTTATTGAATTCAACTGTTTTGTGGTTGTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

304

Amino Acids

33.32

Weight (kDa)

6.26

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 67 - 165 7.4e-25 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 543
AccBSI CCGCTC 2 cut(s) 62, 92
AclWI GGATC 5 cut(s) 217, 230, 245, 494, 507
AcoI YGGCCR 4 cut(s) 3, 383, 564, 645
AcsI RAATTY 4 cut(s) 459, 733, 824, 887
AcuI CTGAAG 1 cut(s) 831
AfaI GTAC 3 cut(s) 306, 663, 853
AfiI CCNNNNNNNGG 1 cut(s) 392
AgsI TTSAA 6 cut(s) 119, 260, 377, 515, 887, 892
AhlI ACTAGT 1 cut(s) 209
AjnI CCWGG 2 cut(s) 157, 218
AjuI GAANNNNNNNTTGG 2 cut(s) 132, 164
AluBI AGCT 4 cut(s) 167, 185, 371, 656
AluI AGCT 4 cut(s) 167, 185, 371, 656
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 1 cut(s) 367
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 5 cut(s) 217, 230, 245, 494, 507
Ama87I CYCGRG 1 cut(s) 503
AoxI GGCC 5 cut(s) 3, 383, 442, 564, 645
ApaLI GTGCAC 1 cut(s) 153
ApeKI GCWGC 2 cut(s) 40, 467
ApoI RAATTY 4 cut(s) 459, 733, 824, 887
AspS9I GGNCC 6 cut(s) 51, 131, 443, 532, 611, 725
AsuHPI GGTGA 1 cut(s) 426
AvaI CYCGRG 1 cut(s) 503
AvaII GGWCC 5 cut(s) 51, 131, 532, 611, 725
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 157
BalI TGGCCA 2 cut(s) 566, 647
BamHI GGATCC 2 cut(s) 222, 499
Bbv12I GWGCWC 1 cut(s) 157
BbvI GCAGC 2 cut(s) 52, 454
BccI CCATC 1 cut(s) 716
BceAI ACGGC 2 cut(s) 5, 398
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 2 cut(s) 159, 220
BclI TGATCA 1 cut(s) 673
BcoDI GTCTC 1 cut(s) 367
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 2 cut(s) 210, 291
BfmI CTRYAG 1 cut(s) 366
BfuAI ACCTGC 1 cut(s) 543
BisI GCNGC 7 cut(s) 6, 35, 38, 41, 90, 403, 468
BlsI GCNGC 7 cut(s) 7, 36, 39, 42, 91, 404, 469
Bme1390I CCNGG 2 cut(s) 159, 220
Bme18I GGWCC 5 cut(s) 51, 131, 532, 611, 725
BmeT110I CYCGRG 1 cut(s) 503
BmgT120I GGNCC 6 cut(s) 51, 131, 443, 532, 611, 725
BmiI GGNNCC 4 cut(s) 133, 224, 501, 612
BmrFI CCNGG 2 cut(s) 159, 220
BmsI GCATC 2 cut(s) 232, 293
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpuEI CTTGAG 1 cut(s) 675
BsaJI CCNNGG 3 cut(s) 135, 218, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse1I ACTGG 3 cut(s) 182, 567, 648
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 2 cut(s) 159, 220
BseDI CCNNGG 3 cut(s) 135, 218, 504
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 3 cut(s) 182, 567, 648
BseRI GAGGAG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 157
BseX3I CGGCCG 1 cut(s) 383
BseXI GCAGC 2 cut(s) 52, 454
Bsh1285I CGRYCG 1 cut(s) 386
BshFI GGCC 5 cut(s) 5, 385, 444, 566, 647
BsiEI CGRYCG 1 cut(s) 386
BsiHKAI GWGCWC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 1 cut(s) 386
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 367
BsmBI CGTCTC 1 cut(s) 367
BsmFI GGGAC 1 cut(s) 624
BsmI GAATGC 1 cut(s) 754
BsnI GGCC 5 cut(s) 5, 385, 444, 566, 647
BsoBI CYCGRG 1 cut(s) 503
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 5 cut(s) 222, 250, 499, 673, 755
Bsp19I CCATGG 1 cut(s) 135
BspANI GGCC 5 cut(s) 5, 385, 444, 566, 647
BspCNI CTCAG 1 cut(s) 520
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 4 cut(s) 133, 224, 501, 612
BspMI ACCTGC 1 cut(s) 543
BspPI GGATC 5 cut(s) 217, 230, 245, 494, 507
BsrBI CCGCTC 2 cut(s) 62, 92
BsrDI GCAATG 1 cut(s) 84
BsrI ACTGG 3 cut(s) 182, 567, 648
BssECI CCNNGG 3 cut(s) 135, 218, 504
BssMI GATC 5 cut(s) 222, 250, 499, 673, 755
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 2 cut(s) 159, 220
Bst4CI ACNGT 1 cut(s) 896
Bst6I CTCTTC 1 cut(s) 510
BstDEI CTNAG 1 cut(s) 528
BstDSI CCRYGG 1 cut(s) 135
BstF5I GGATG 1 cut(s) 829
BstKTI GATC 5 cut(s) 225, 253, 502, 676, 758
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 5 cut(s) 222, 250, 499, 673, 755
BstMCI CGRYCG 1 cut(s) 386
BstMWI GCNNNNNNNGC 7 cut(s) 40, 68, 95, 104, 473, 653, 806
BstNI CCWGG 2 cut(s) 159, 220
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 2 cut(s) 157, 218
BstSFI CTRYAG 1 cut(s) 366
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 52, 454
BstX2I RGATCY 2 cut(s) 222, 499
BstYI RGATCY 2 cut(s) 222, 499
BstZI CGGCCG 1 cut(s) 383
Bsu36I CCTNAGG 1 cut(s) 528
BsuRI GGCC 5 cut(s) 5, 385, 444, 566, 647
BtgI CCRYGG 1 cut(s) 135
BtsCI GGATG 1 cut(s) 829
BtsIMutI CAGTG 1 cut(s) 171
BveI ACCTGC 1 cut(s) 543
CciI TCATGA 1 cut(s) 598
Cfr13I GGNCC 6 cut(s) 51, 131, 443, 532, 611, 725
CsiI ACCWGGT 1 cut(s) 157
Csp6I GTAC 3 cut(s) 305, 662, 852
CviAII CATG 7 cut(s) 55, 136, 316, 406, 599, 643, 659
CviQI GTAC 3 cut(s) 305, 662, 852
DdeI CTNAG 1 cut(s) 528
DpnI GATC 5 cut(s) 224, 252, 501, 675, 757
DpnII GATC 5 cut(s) 222, 250, 499, 673, 755
EaeI YGGCCR 4 cut(s) 3, 383, 564, 645
EagI CGGCCG 1 cut(s) 383
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
EciI GGCGGA 2 cut(s) 21, 621
EclXI CGGCCG 1 cut(s) 383
Eco130I CCWWGG 1 cut(s) 135
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 5 cut(s) 51, 131, 532, 611, 725
Eco52I CGGCCG 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 831
Eco81I CCTNAGG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 503
EcoO109I RGGNCCY 2 cut(s) 532, 611
EcoRI GAATTC 2 cut(s) 733, 887
EcoRII CCWGG 2 cut(s) 157, 218
EcoRV GATATC 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 367
FaeI CATG 7 cut(s) 58, 139, 319, 409, 602, 646, 662
FaqI GGGAC 1 cut(s) 624
FatI CATG 7 cut(s) 54, 135, 315, 405, 598, 642, 658
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 7 cut(s) 6, 35, 38, 41, 90, 403, 468
FokI GGATG 1 cut(s) 816
Fsp4HI GCNGC 7 cut(s) 6, 35, 38, 41, 90, 403, 468
FspBI CTAG 2 cut(s) 210, 291
GluI GCNGC 7 cut(s) 6, 35, 38, 41, 90, 403, 468
HaeIII GGCC 5 cut(s) 5, 385, 444, 566, 647
HapII CCGG 1 cut(s) 386
Hin1II CATG 7 cut(s) 58, 139, 319, 409, 602, 646, 662
HindIII AAGCTT 1 cut(s) 165
HinfI GANTC 3 cut(s) 75, 575, 776
HpaII CCGG 1 cut(s) 386
HphI GGTGA 1 cut(s) 426
Hpy166II GTNNAC 2 cut(s) 155, 668
Hpy188I TCNGA 1 cut(s) 772
Hpy188III TCNNGA 6 cut(s) 65, 503, 527, 599, 719, 833
Hpy8I GTNNAC 2 cut(s) 155, 668
HpyAV CCTTC 1 cut(s) 42
HpyCH4III ACNGT 1 cut(s) 896
HpyCH4IV ACGT 3 cut(s) 303, 554, 664
HpyCH4V TGCA 7 cut(s) 155, 231, 284, 470, 476, 752, 800
HpyF10VI GCNNNNNNNGC 7 cut(s) 40, 68, 95, 104, 473, 653, 806
HpyF3I CTNAG 1 cut(s) 528
HpySE526I ACGT 3 cut(s) 303, 554, 664
Hsp92II CATG 7 cut(s) 58, 139, 319, 409, 602, 646, 662
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 5 cut(s) 222, 250, 499, 673, 755
Lsp1109I GCAGC 2 cut(s) 52, 454
LweI GCATC 2 cut(s) 232, 293
MabI ACCWGGT 1 cut(s) 157
MaeI CTAG 2 cut(s) 210, 291
MaeII ACGT 3 cut(s) 303, 554, 664
MaeIII GTNAC 1 cut(s) 764
MalI GATC 5 cut(s) 224, 252, 501, 675, 757
MbiI CCGCTC 2 cut(s) 62, 92
MboI GATC 5 cut(s) 222, 250, 499, 673, 755
MboII GAAGA 4 cut(s) 18, 57, 527, 837
MfeI CAATTG 2 cut(s) 471, 684
MflI RGATCY 2 cut(s) 222, 499
MhlI GDGCHC 1 cut(s) 157
MlsI TGGCCA 2 cut(s) 566, 647
MluNI TGGCCA 2 cut(s) 566, 647
Mox20I TGGCCA 2 cut(s) 566, 647
MscI TGGCCA 2 cut(s) 566, 647
MseI TTAA 2 cut(s) 203, 410
MslI CAYNNNNRTG 1 cut(s) 771
Msp20I TGGCCA 2 cut(s) 566, 647
MspI CCGG 1 cut(s) 386
MspR9I CCNGG 2 cut(s) 159, 220
MunI CAATTG 2 cut(s) 471, 684
Mva1269I GAATGC 1 cut(s) 754
MvaI CCWGG 2 cut(s) 159, 220
MwoI GCNNNNNNNGC 7 cut(s) 40, 68, 95, 104, 473, 653, 806
NcoI CCATGG 1 cut(s) 135
NdeII GATC 5 cut(s) 222, 250, 499, 673, 755
NlaIII CATG 7 cut(s) 58, 139, 319, 409, 602, 646, 662
NlaIV GGNNCC 4 cut(s) 133, 224, 501, 612
NmuCI GTSAC 1 cut(s) 764
NspI RCATGY 1 cut(s) 409
PagI TCATGA 1 cut(s) 598
PctI GAATGC 1 cut(s) 754
PfeI GAWTC 3 cut(s) 75, 575, 776
PflFI GACNNNGTC 1 cut(s) 55
PkrI GCNGC 7 cut(s) 7, 36, 39, 42, 91, 404, 469
PpuMI RGGWCCY 2 cut(s) 532, 611
Psp5II RGGWCCY 2 cut(s) 532, 611
Psp6I CCWGG 2 cut(s) 157, 218
PspGI CCWGG 2 cut(s) 157, 218
PspN4I GGNNCC 4 cut(s) 133, 224, 501, 612
PspPI GGNCC 6 cut(s) 51, 131, 443, 532, 611, 725
PspPPI RGGWCCY 2 cut(s) 532, 611
PsuI RGATCY 2 cut(s) 222, 499
PsyI GACNNNGTC 1 cut(s) 55
RsaI GTAC 3 cut(s) 306, 663, 853
RsaNI GTAC 3 cut(s) 305, 662, 852
RseI CAYNNNNRTG 1 cut(s) 771
SaqAI TTAA 2 cut(s) 203, 410
SatI GCNGC 7 cut(s) 6, 35, 38, 41, 90, 403, 468
Sau3AI GATC 5 cut(s) 222, 250, 499, 673, 755
Sau96I GGNCC 6 cut(s) 51, 131, 443, 532, 611, 725
ScrFI CCNGG 2 cut(s) 159, 220
SduI GDGCHC 1 cut(s) 157
SexAI ACCWGGT 1 cut(s) 157
SfaNI GCATC 2 cut(s) 232, 293
SfcI CTRYAG 1 cut(s) 366
SinI GGWCC 5 cut(s) 51, 131, 532, 611, 725
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 690
SmoI CTYRAG 1 cut(s) 690
SpeI ACTAGT 1 cut(s) 209
SspMI CTAG 2 cut(s) 210, 291
StyD4I CCNGG 2 cut(s) 157, 218
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 896
TaiI ACGT 3 cut(s) 306, 557, 667
TaqI TCGA 1 cut(s) 66
TauI GCSGC 5 cut(s) 8, 37, 40, 92, 405
TfiI GAWTC 3 cut(s) 75, 575, 776
Tru1I TTAA 2 cut(s) 203, 410
Tru9I TTAA 2 cut(s) 203, 410
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 1 cut(s) 764
TseI GCWGC 2 cut(s) 40, 467
Tsp45I GTSAC 1 cut(s) 764
TspDTI ATGAA 3 cut(s) 470, 746, 818
TspRI CASTG 1 cut(s) 178
Tth111I GACNNNGTC 1 cut(s) 55
VneI GTGCAC 1 cut(s) 153
VpaK11BI GGWCC 5 cut(s) 51, 131, 532, 611, 725
XapI RAATTY 4 cut(s) 459, 733, 824, 887
XceI RCATGY 1 cut(s) 409
XspI CTAG 2 cut(s) 210, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.