Rw2G005800

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008176
Physical Location & Seq
Forward (+)
5528052 .. 5533549
5498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005800.1

Sequence Viewer

Length: 1860 bp
ATGGATAAAGCTTTCCTTCTTAAGCTCATATGTCATACAACCGTACAATTCAAGCAGAAGGTTTCTATGCTTGATAACTCTGGGAAGCCAGTCCTTGCATCAACTGACAGTTCAAACCCCTGGTGCGCCCTGCTAGAAGAAGTGGTCAGTAAAGCTTACAGAACACTTGGTAAGCCTGAGATTTGTCCTGCTTCAACAGATGCTCACTATTTCCAGCTACAAGGCTTGCCAGCAATTGGATTCTCTCCTATGGCCAACACTCATTTTCTGCTCCATGACCATAACGAGGTCGTGGCTGTTGGAAGATTCCAAAATGGTGGTGCATTCAATGTTATTCCAGATTCTGCTGAACAAAGGGTTGAAGAGGTTGAACATGTGGTAAGGGAAGAGAAGTTGAGGGAAGCATATGAGCTTATTGAGATATATTGCGAACTTATTGCAGCACGATTGCCAATGTTTGAGTCGCAGAAGAACTGCCTAATTGATCTGGAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATGGATATCCACAAGCATTTCACAGCAAAATATGGAAAGGAGTTCGTCTCTGCAGCTGTTGAACTGCGCCCAGATAGTGTTCAACCTCCGATTGCTCAAGTTCCACGCAGAAATTATGAGTATCATGATGGACCTATGAATTCTAATGAGCAGAATGCAAGATCATCACCTCGCTTGGAGGATTCTGCATCTACAAATGTTTCTGCCAACAAAGCAACCACATCTGCCAAATTTCATCCAGAAACGACATCTTCAGGTACATCAATTTGTCACATAAAGATTGTGAAACCAGAAGCAGTCGAATGGAAATGCATTTATTTCCGGGGGACTTCCTCCCCATCTAATTCTAGTGTTGGTAGCGATCATCAGGAAGACGTTTTGAAAGAGGATGATCATATTGATTACTTTGCAGATTCTGGTTCAGACGATGATAATTACAAGGGGCAAGAATCAAGTTTGTTTTTTTCTTCTCCTGCTAGAAATTCATTTCCTTCAGCTAGTTTAGATCCTTGGAGTTTTGAGCAAAAGAGAGATGAGGTACCATTCAAGTCCGTTTCACCATTACGTTCTTCATCAGTGCAGCACTCCCTTCCCATCTTTTCTGAAGGTTTGACAGGTTCTACAGTCCCTTCGGAACCAAATGACTTCGTGCCTGTTACTTTTGATGCTTCAGATGGCCCAAGTTCAGACAGTGAGGAGGAGTTGGACACGTCTATGCTTGTCCTGAGCAAAGATTCTAAATTTTCTCATGAGAAGACTGTTCAATCCATAAATTCGGGAAAAAGCCAGAGTGCAAGCCACAGGTCATTAGCATCTTCATCCTCTGATGAGAGGATGAACTCTCACATGAAACTCTTAGCAATAGTCGCCAAGACCTGTCCCATCATAAATCGGTATCCCGAGGAAAGAGAAGCTCAACGTCTGCATTTTTCTTTAATTCAGAGGAGAGTGAGGCTGAGGAAGATCTTCCTAGAAAGATTTCCACCACCATTGCTCGTCCAAGTCAAAAATTTTCTAGAAGGACACAACCCTCTTCTTCAAATTCTGTTAGAACTTAGAAGTTCTAGCTTAAGGACTACAGTTGTTCCTGATGCACCAAGGACTTCAGACTATGGAAAATCCTCTTCAAGAAGTTCCAACGACACTGAAACTCTACCAGAGCCCTCAGGAAGTCAGGATTGGCAGAGACCTATAGAACAAACAAGTTCTTATTCAATTCCAGAATCCAAGAAGACTAGGACATCAGTCAATGGAAATTCCTCCTCAAGAAGTTCTTATGCCACTGAAACTCTACCAAGTGCCTCATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

619

Amino Acids

68.69

Weight (kDa)

5.58

Isoelectric Point (pI)

58.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 116 - 215 2e-30 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1087
AccB1I GGYRCC 1 cut(s) 1087
AccB7I CCANNNNNTGG 1 cut(s) 236
AciI CCGC 1 cut(s) 533
AclWI GGATC 1 cut(s) 1049
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 8 cut(s) 688, 779, 1030, 1289, 1321, 1558, 1590, 1804
AcuI CTGAAG 5 cut(s) 786, 1026, 1173, 1203, 1638
AfaI GTAC 4 cut(s) 45, 540, 808, 1089
AfiI CCNNNNNNNGG 2 cut(s) 236, 286
AflII CTTAAG 2 cut(s) 20, 1618
AflIII ACRYGT 2 cut(s) 373, 1257
AjiI CACGTC 1 cut(s) 1260
AjnI CCWGG 1 cut(s) 119
AjuI GAANNNNNNNTTGG 2 cut(s) 1711, 1743
AluBI AGCT 9 cut(s) 11, 25, 155, 217, 412, 605, 1046, 1463, 1617
AluI AGCT 9 cut(s) 11, 25, 155, 217, 412, 605, 1046, 1463, 1617
Alw26I GTCTC 2 cut(s) 601, 1729
AlwI GGATC 1 cut(s) 1049
AlwNI CAGNNNCTG 2 cut(s) 344, 965
Ama87I CYCGRG 1 cut(s) 1448
AoxI GGCC 2 cut(s) 252, 1225
ApeKI GCWGC 3 cut(s) 440, 602, 1129
ApoI RAATTY 8 cut(s) 688, 779, 1030, 1289, 1321, 1558, 1590, 1804
Asp700I GAANNNNTTC 2 cut(s) 1514, 1527
Asp718I GGTACC 1 cut(s) 1087
AspLEI GCGC 2 cut(s) 128, 618
AspS9I GGNCC 2 cut(s) 680, 1226
AsuC2I CCSGG 1 cut(s) 872
AsuHPI GGTGA 2 cut(s) 708, 1098
AvaI CYCGRG 1 cut(s) 1448
AvaII GGWCC 1 cut(s) 680
AxyI CCTNAGG 1 cut(s) 1714
BalI TGGCCA 1 cut(s) 254
BanI GGYRCC 1 cut(s) 1087
BanII GRGCYC 1 cut(s) 1713
BbsI GAAGAC 3 cut(s) 927, 1310, 1787
BbvCI CCTCAGC 1 cut(s) 1505
BbvI GCAGC 3 cut(s) 452, 614, 1141
BccI CCATC 5 cut(s) 671, 895, 1151, 1217, 1439
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 1 cut(s) 121
BciVI GTATCC 1 cut(s) 1455
BclI TGATCA 1 cut(s) 940
BcnI CCSGG 1 cut(s) 872
BcoDI GTCTC 2 cut(s) 601, 1729
BfaI CTAG 9 cut(s) 134, 525, 897, 1026, 1047, 1520, 1565, 1614, 1785
BfmI CTRYAG 4 cut(s) 600, 1170, 1626, 1740
BfrI CTTAAG 2 cut(s) 20, 1618
BfuI GTATCC 1 cut(s) 1455
BglII AGATCT 1 cut(s) 1512
BisI GCNGC 3 cut(s) 441, 603, 1130
BlsI GCNGC 3 cut(s) 442, 604, 1131
Bme1390I CCNGG 2 cut(s) 121, 872
Bme18I GGWCC 1 cut(s) 680
BmeT110I CYCGRG 1 cut(s) 1448
BmgBI CACGTC 1 cut(s) 1260
BmgT120I GGNCC 2 cut(s) 680, 1226
BmiI GGNNCC 2 cut(s) 1089, 1185
BmrFI CCNGG 2 cut(s) 121, 872
BmsI GCATC 7 cut(s) 107, 190, 527, 746, 1204, 1370, 1630
BoxI GACNNNNGTC 1 cut(s) 1793
BpiI GAAGAC 3 cut(s) 927, 1310, 1787
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 1 cut(s) 509
Bpu10I CCTNAGC 2 cut(s) 1274, 1505
BpuEI CTTGAG 2 cut(s) 630, 1798
BpuMI CCSGG 1 cut(s) 872
BsaI GGTCTC 1 cut(s) 1729
BsaJI CCNNGG 5 cut(s) 119, 871, 1058, 1449, 1646
Bsc4I CCNNNNNNNGG 2 cut(s) 236, 286
Bse1I ACTGG 1 cut(s) 89
Bse21I CCTNAGG 1 cut(s) 1714
Bse3DI GCAATG 1 cut(s) 1538
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 5 cut(s) 119, 871, 1058, 1449, 1646
BseGI GGATG 4 cut(s) 784, 943, 1367, 1389
BseLI CCNNNNNNNGG 2 cut(s) 236, 286
BseMI GCAATG 1 cut(s) 1538
BseMII CTCAG 4 cut(s) 168, 1265, 1496, 1728
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 4 cut(s) 1259, 1262, 1507, 1801
BseXI GCAGC 3 cut(s) 452, 614, 1141
BsgI GTGCAG 1 cut(s) 1148
BshFI GGCC 2 cut(s) 254, 1227
BshNI GGYRCC 1 cut(s) 1087
BsiHKCI CYCGRG 1 cut(s) 1448
BsiSI CCGG 1 cut(s) 871
BslFI GGGAC 3 cut(s) 889, 1160, 1413
BslI CCNNNNNNNGG 2 cut(s) 236, 286
BsmAI GTCTC 2 cut(s) 601, 1729
BsmBI CGTCTC 1 cut(s) 601
BsmFI GGGAC 3 cut(s) 889, 1160, 1413
BsmI GAATGC 2 cut(s) 323, 709
BsnI GGCC 2 cut(s) 254, 1227
Bso31I GGTCTC 1 cut(s) 1729
BsoBI CYCGRG 1 cut(s) 1448
Bsp1286I GDGCHC 1 cut(s) 1713
Bsp143I GATC 6 cut(s) 484, 710, 910, 940, 1054, 1512
BspACI CCGC 1 cut(s) 533
BspANI GGCC 2 cut(s) 254, 1227
BspCNI CTCAG 4 cut(s) 169, 1266, 1497, 1727
BspHI TCATGA 2 cut(s) 673, 1297
BspLI GGNNCC 2 cut(s) 1089, 1185
BspMAI CTGCAG 1 cut(s) 604
BspPI GGATC 1 cut(s) 1049
BspT107I GGYRCC 1 cut(s) 1087
BspTI CTTAAG 2 cut(s) 20, 1618
BspTNI GGTCTC 1 cut(s) 1729
BsrDI GCAATG 1 cut(s) 1538
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 5 cut(s) 119, 871, 1058, 1449, 1646
BssMI GATC 6 cut(s) 484, 710, 910, 940, 1054, 1512
BssT1I CCWWGG 2 cut(s) 1058, 1646
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 6 cut(s) 43, 110, 1174, 1241, 1309, 1630
Bst6I CTCTTC 4 cut(s) 357, 381, 1587, 1678
BstAFI CTTAAG 2 cut(s) 20, 1618
BstC8I GCNNGC 3 cut(s) 227, 231, 1345
BstDEI CTNAG 6 cut(s) 177, 1274, 1405, 1505, 1604, 1714
BstF5I GGATG 4 cut(s) 784, 943, 1367, 1389
BstHHI GCGC 2 cut(s) 128, 618
BstKTI GATC 6 cut(s) 487, 713, 913, 943, 1057, 1515
BstMAI GTCTC 2 cut(s) 601, 1729
BstMBI GATC 6 cut(s) 484, 710, 910, 940, 1054, 1512
BstMWI GCNNNNNNNGC 2 cut(s) 761, 1415
BstNI CCWGG 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 377
BstPAI GACNNNNGTC 1 cut(s) 1793
BstSCI CCNGG 2 cut(s) 119, 870
BstSFI CTRYAG 4 cut(s) 600, 1170, 1626, 1740
BstV1I GCAGC 3 cut(s) 452, 614, 1141
BstV2I GAAGAC 3 cut(s) 927, 1310, 1787
BstX2I RGATCY 2 cut(s) 1054, 1512
BstXI CCANNNNNNTGG 1 cut(s) 317
BstYI RGATCY 2 cut(s) 1054, 1512
Bsu36I CCTNAGG 1 cut(s) 1714
BsuI GTATCC 1 cut(s) 1455
BsuRI GGCC 2 cut(s) 254, 1227
BtrI CACGTC 1 cut(s) 1260
BtsCI GGATG 4 cut(s) 784, 943, 1367, 1389
BtsIMutI CAGTG 4 cut(s) 1131, 1246, 1692, 1830
Cac8I GCNNGC 3 cut(s) 227, 231, 1345
CaiI CAGNNNCTG 2 cut(s) 344, 965
CciI TCATGA 2 cut(s) 673, 1297
CfoI GCGC 2 cut(s) 128, 618
Cfr13I GGNCC 2 cut(s) 680, 1226
Csp6I GTAC 4 cut(s) 44, 539, 807, 1088
CspCI CAANNNNNGTGG 2 cut(s) 1521, 1556
CviAII CATG 6 cut(s) 275, 374, 550, 674, 1298, 1396
CviQI GTAC 4 cut(s) 44, 539, 807, 1088
DdeI CTNAG 6 cut(s) 177, 1274, 1405, 1505, 1604, 1714
DpnI GATC 6 cut(s) 486, 712, 912, 942, 1056, 1514
DpnII GATC 6 cut(s) 484, 710, 910, 940, 1054, 1512
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 4 cut(s) 357, 381, 1587, 1678
EarI CTCTTC 4 cut(s) 357, 381, 1587, 1678
Eco130I CCWWGG 2 cut(s) 1058, 1646
Eco24I GRGCYC 1 cut(s) 1713
Eco31I GGTCTC 1 cut(s) 1729
Eco32I GATATC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 680
Eco57I CTGAAG 5 cut(s) 786, 1026, 1173, 1203, 1638
Eco81I CCTNAGG 1 cut(s) 1714
Eco88I CYCGRG 1 cut(s) 1448
EcoRI GAATTC 1 cut(s) 688
EcoRII CCWGG 1 cut(s) 119
EcoRV GATATC 1 cut(s) 556
EcoT14I CCWWGG 2 cut(s) 1058, 1646
EcoT22I ATGCAT 1 cut(s) 863
EcoT38I GRGCYC 1 cut(s) 1713
ErhI CCWWGG 2 cut(s) 1058, 1646
Esp3I CGTCTC 1 cut(s) 601
FaeI CATG 6 cut(s) 278, 377, 553, 677, 1301, 1399
FalI AAGNNNNNCTT 3 cut(s) 32, 1807, 1839
FaqI GGGAC 3 cut(s) 889, 1160, 1413
FatI CATG 6 cut(s) 274, 373, 549, 673, 1297, 1395
FauNDI CATATG 2 cut(s) 29, 406
FbaI TGATCA 1 cut(s) 940
Fnu4HI GCNGC 3 cut(s) 441, 603, 1130
FokI GGATG 4 cut(s) 771, 950, 1354, 1396
FriOI GRGCYC 1 cut(s) 1713
Fsp4HI GCNGC 3 cut(s) 441, 603, 1130
FspBI CTAG 9 cut(s) 134, 525, 897, 1026, 1047, 1520, 1565, 1614, 1785
GlaI GCGC 2 cut(s) 127, 617
GluI GCNGC 3 cut(s) 441, 603, 1130
GsuI CTGGAG 1 cut(s) 509
HaeIII GGCC 2 cut(s) 254, 1227
HapII CCGG 1 cut(s) 871
HhaI GCGC 2 cut(s) 128, 618
Hin1II CATG 6 cut(s) 278, 377, 553, 677, 1301, 1399
Hin6I GCGC 2 cut(s) 126, 616
HinP1I GCGC 2 cut(s) 126, 616
HindIII AAGCTT 2 cut(s) 9, 153
HinfI GANTC 9 cut(s) 240, 306, 341, 461, 731, 962, 998, 1283, 1772
HpaII CCGG 1 cut(s) 871
HphI GGTGA 2 cut(s) 708, 1098
Hpy188I TCNGA 9 cut(s) 639, 973, 1153, 1183, 1222, 1237, 1375, 1491, 1657
HpyAV CCTTC 7 cut(s) 26, 52, 1050, 1148, 1148, 1188, 1562
HpyCH4III ACNGT 6 cut(s) 43, 110, 1174, 1241, 1309, 1630
HpyCH4IV ACGT 5 cut(s) 537, 924, 1114, 1259, 1468
HpyF10VI GCNNNNNNNGC 2 cut(s) 761, 1415
HpyF3I CTNAG 6 cut(s) 177, 1274, 1405, 1505, 1604, 1714
HpySE526I ACGT 5 cut(s) 537, 924, 1114, 1259, 1468
Hsp92II CATG 6 cut(s) 278, 377, 553, 677, 1301, 1399
HspAI GCGC 2 cut(s) 126, 616
KpnI GGTACC 1 cut(s) 1091
Ksp22I TGATCA 1 cut(s) 940
Kzo9I GATC 6 cut(s) 484, 710, 910, 940, 1054, 1512
LmnI GCTCC 1 cut(s) 276
Lsp1109I GCAGC 3 cut(s) 452, 614, 1141
LweI GCATC 7 cut(s) 107, 190, 527, 746, 1204, 1370, 1630
MaeI CTAG 9 cut(s) 134, 525, 897, 1026, 1047, 1520, 1565, 1614, 1785
MaeII ACGT 5 cut(s) 537, 924, 1114, 1259, 1468
MaeIII GTNAC 2 cut(s) 818, 1204
MalI GATC 6 cut(s) 486, 712, 912, 942, 1056, 1514
MboI GATC 6 cut(s) 484, 710, 910, 940, 1054, 1512
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 2 cut(s) 1054, 1512
MhlI GDGCHC 1 cut(s) 1713
MlsI TGGCCA 1 cut(s) 254
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 3 cut(s) 280, 1233, 1710
Mox20I TGGCCA 1 cut(s) 254
Mph1103I ATGCAT 1 cut(s) 863
MroXI GAANNNNTTC 2 cut(s) 1514, 1527
MscI TGGCCA 1 cut(s) 254
MseI TTAA 3 cut(s) 21, 1484, 1619
MslI CAYNNNNRTG 1 cut(s) 1262
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 605
MspCI CTTAAG 2 cut(s) 20, 1618
MspI CCGG 1 cut(s) 871
MspR9I CCNGG 2 cut(s) 121, 872
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 2 cut(s) 323, 709
MvaI CCWGG 1 cut(s) 121
MwoI GCNNNNNNNGC 2 cut(s) 761, 1415
NciI CCSGG 1 cut(s) 872
NdeI CATATG 2 cut(s) 29, 406
NdeII GATC 6 cut(s) 484, 710, 910, 940, 1054, 1512
NlaIII CATG 6 cut(s) 278, 377, 553, 677, 1301, 1399
NlaIV GGNNCC 2 cut(s) 1089, 1185
NmuCI GTSAC 1 cut(s) 818
NsiI ATGCAT 1 cut(s) 863
NspI RCATGY 1 cut(s) 377
PagI TCATGA 2 cut(s) 673, 1297
PciI ACATGT 1 cut(s) 373
PctI GAATGC 2 cut(s) 323, 709
PdmI GAANNNNTTC 2 cut(s) 1514, 1527
PfeI GAWTC 8 cut(s) 240, 306, 341, 731, 962, 998, 1283, 1772
PflMI CCANNNNNTGG 1 cut(s) 236
PkrI GCNGC 3 cut(s) 442, 604, 1131
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PscI ACATGT 1 cut(s) 373
PshAI GACNNNNGTC 1 cut(s) 1793
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 1089, 1185
PspPI GGNCC 2 cut(s) 680, 1226
PstI CTGCAG 1 cut(s) 604
PstNI CAGNNNCTG 2 cut(s) 344, 965
PsuI RGATCY 2 cut(s) 1054, 1512
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 4 cut(s) 45, 540, 808, 1089
RsaNI GTAC 4 cut(s) 44, 539, 807, 1088
RseI CAYNNNNRTG 1 cut(s) 1262
SaqAI TTAA 3 cut(s) 21, 1484, 1619
SatI GCNGC 3 cut(s) 441, 603, 1130
Sau3AI GATC 6 cut(s) 484, 710, 910, 940, 1054, 1512
Sau96I GGNCC 2 cut(s) 680, 1226
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 121, 872
SduI GDGCHC 1 cut(s) 1713
SfaNI GCATC 7 cut(s) 107, 190, 527, 746, 1204, 1370, 1630
SfcI CTRYAG 4 cut(s) 600, 1170, 1626, 1740
SinI GGWCC 1 cut(s) 680
SmiMI CAYNNNNRTG 1 cut(s) 1262
SmlI CTYRAG 4 cut(s) 20, 645, 1618, 1813
SmoI CTYRAG 4 cut(s) 20, 645, 1618, 1813
SsiI CCGC 1 cut(s) 533
SspMI CTAG 9 cut(s) 134, 525, 897, 1026, 1047, 1520, 1565, 1614, 1785
StyD4I CCNGG 2 cut(s) 119, 870
StyI CCWWGG 2 cut(s) 1058, 1646
TaaI ACNGT 6 cut(s) 43, 110, 1174, 1241, 1309, 1630
TaiI ACGT 5 cut(s) 540, 927, 1117, 1262, 1471
TaqI TCGA 1 cut(s) 849
TfiI GAWTC 8 cut(s) 240, 306, 341, 731, 962, 998, 1283, 1772
Tru1I TTAA 3 cut(s) 21, 1484, 1619
Tru9I TTAA 3 cut(s) 21, 1484, 1619
TscAI CASTG 4 cut(s) 1131, 1246, 1699, 1837
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 3 cut(s) 440, 602, 1129
Tsp45I GTSAC 1 cut(s) 818
TspDTI ATGAA 7 cut(s) 701, 773, 1023, 1110, 1356, 1400, 1412
TspGWI ACGGA 1 cut(s) 1090
TspRI CASTG 4 cut(s) 1131, 1246, 1699, 1837
Van91I CCANNNNNTGG 1 cut(s) 236
Vha464I CTTAAG 2 cut(s) 20, 1618
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 8 cut(s) 688, 779, 1030, 1289, 1321, 1558, 1590, 1804
XbaI TCTAGA 1 cut(s) 1564
XceI RCATGY 1 cut(s) 377
XmnI GAANNNNTTC 2 cut(s) 1514, 1527
XspI CTAG 9 cut(s) 134, 525, 897, 1026, 1047, 1520, 1565, 1614, 1785
Zsp2I ATGCAT 1 cut(s) 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.