Rh2AG075100

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008176
Physical Location & Seq
Reverse (-)
6284742 .. 6287827
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG075100.1

Sequence Viewer

Length: 1215 bp
ATGGTTTGTGACGATGGCTTTTGCCCTGACCCGGGCTATCAGGCCCTCAGCAAAGGCTTGGACATTATACAACAGCCATCTGAAGCCGAAGCCGGGCATCAGGAGTCTTTGGAGAAACGTATTGCTGAAGAATGGATGCCTTCTTCACGGAACATGACATATATGCTTGCGCAGTTCAAGCAGAAGGTTTCTATGCTTGATAACTCTGGGAAGCCAGTCCTTGCATCAACTGACAGTTCAAACCCCTGGTGCGCCCTGCTAGAAGAAGTGGTCAGTAAAGCTTACGGAACACTTGGTAAGCCTGAGATTTGTCCTGCTTCAACAGATGCTCACTATTTCCAGCTACAAGGCTTGCCAGCAATTGGATTCTCTCCTATGGCCAACACTCATTTTCTGCTCCATGACCATAACGAGGTCGTGGCTGTTGGAAGATTCCAAAATGGTGGTGCATTCAATGTTATTCCAGATTCTGCTGAACAAAGGGTTGAAGAGAGCTTGATTCGTGTTGGTTTGTTTTATTTTCTTTACAGGAACTGCCTAATTGATCTGGAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATGGATATCCACAAGCATTTCACAGCAAAATATGGAAAGGAGTTCGTCTCTGCAGCTGTTGAACTGCGCCCAGATAGTGGTGTAAGCCGCATGGTAATAGAAAAATTATCCACCAAATCACCTGATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTGCTGAGGACCTGCTGAACGGACCAAATACTTTTGGCATGGCTTCTGCTACACCATCACCTACTCATGATGTCAGGGGACCTACTAATGTCCAAGTTCCTCCTAATAGTAGCATGGCCAATAAAGCTCATGTGGAAGCTCCCCCTGGTCCGCTTCCACCTGATAAACCTCCTAATGCTGAAGTTCCACGCAGAAATTATGAGTTTCAGGATGGACCCGTGAACTCTAATGAGCAGATTGCAAGATCATCACCTCGCTTGGAGGATCCTGCTTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTACCACATTTCATCCAGAAACGATATCCTCAGGAAGTGATCGAATGGAAATGCATGGGGATGATAATTCTTCGGGCAGGCAGAACTGGAATATGCAATTTAAGGGTACTGCCTCTGCAGCACAGGCAGCTGCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

404

Amino Acids

44.05

Weight (kDa)

4.97

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 177 - 247 1.2e-20 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 171
Acc36I ACCTGC 1 cut(s) 789
AccB7I CCANNNNNTGG 2 cut(s) 362, 686
AciI CCGC 3 cut(s) 593, 697, 920
AclWI GGATC 4 cut(s) 740, 753, 1028, 1041
AcoI YGGCCR 2 cut(s) 378, 885
AcuI CTGAAG 3 cut(s) 102, 147, 969
AfaI GTAC 2 cut(s) 600, 1183
AfiI CCNNNNNNNGG 6 cut(s) 31, 32, 93, 362, 412, 686
AgsI TTSAA 7 cut(s) 178, 240, 321, 454, 488, 671, 761
AjnI CCWGG 2 cut(s) 245, 913
AluBI AGCT 7 cut(s) 281, 343, 495, 665, 896, 908, 1205
AluI AGCT 7 cut(s) 281, 343, 495, 665, 896, 908, 1205
Alw26I GTCTC 1 cut(s) 661
AlwI GGATC 4 cut(s) 740, 753, 1028, 1041
AlwNI CAGNNNCTG 3 cut(s) 470, 534, 1208
Ama87I CYCGRG 2 cut(s) 31, 749
AoxI GGCC 3 cut(s) 42, 378, 885
ApeKI GCWGC 4 cut(s) 662, 1193, 1202, 1205
AspLEI GCGC 3 cut(s) 172, 254, 678
AspS9I GGNCC 6 cut(s) 43, 778, 791, 848, 917, 983
AsuC2I CCSGG 3 cut(s) 32, 33, 94
AsuHPI GGTGA 3 cut(s) 720, 819, 1011
AvaI CYCGRG 2 cut(s) 31, 749
AvaII GGWCC 5 cut(s) 778, 791, 848, 917, 983
AxyI CCTNAGG 1 cut(s) 1105
BalI TGGCCA 2 cut(s) 380, 887
BamHI GGATCC 2 cut(s) 745, 1033
BbvCI CCTCAGC 2 cut(s) 47, 774
BbvI GCAGC 3 cut(s) 674, 1192, 1205
BccI CCATC 4 cut(s) 8, 85, 832, 974
BciT130I CCWGG 2 cut(s) 247, 915
BcnI CCSGG 3 cut(s) 32, 33, 94
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 2 cut(s) 260, 585
BfmI CTRYAG 2 cut(s) 660, 1191
BfuAI ACCTGC 1 cut(s) 789
BisI GCNGC 5 cut(s) 663, 697, 1194, 1203, 1206
BlsI GCNGC 5 cut(s) 664, 698, 1195, 1204, 1207
Bme1390I CCNGG 5 cut(s) 32, 33, 94, 247, 915
Bme18I GGWCC 5 cut(s) 778, 791, 848, 917, 983
BmeT110I CYCGRG 2 cut(s) 31, 749
BmgT120I GGNCC 6 cut(s) 43, 778, 791, 848, 917, 983
BmiI GGNNCC 4 cut(s) 747, 849, 985, 1035
BmrFI CCNGG 5 cut(s) 32, 33, 94, 247, 915
BmsI GCATC 5 cut(s) 106, 126, 233, 316, 587
BplI GAGNNNNNCTC 2 cut(s) 641, 673
BpmI CTGGAG 1 cut(s) 569
Bpu10I CCTNAGC 2 cut(s) 47, 774
BpuMI CCSGG 3 cut(s) 32, 33, 94
BsaJI CCNNGG 4 cut(s) 31, 245, 750, 913
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 32, 93, 362, 412, 686
Bse1I ACTGG 2 cut(s) 215, 1166
Bse21I CCTNAGG 1 cut(s) 1105
BseBI CCWGG 2 cut(s) 247, 915
BseDI CCNNGG 4 cut(s) 31, 245, 750, 913
BseGI GGATG 4 cut(s) 141, 985, 1087, 1141
BseLI CCNNNNNNNGG 6 cut(s) 31, 32, 93, 362, 412, 686
BseMII CTCAG 4 cut(s) 61, 294, 765, 1119
BseNI ACTGG 2 cut(s) 215, 1166
BseXI GCAGC 3 cut(s) 674, 1192, 1205
BshFI GGCC 3 cut(s) 44, 380, 887
BsiHKCI CYCGRG 2 cut(s) 31, 749
BsiSI CCGG 2 cut(s) 32, 93
BslFI GGGAC 1 cut(s) 861
BslI CCNNNNNNNGG 6 cut(s) 31, 32, 93, 362, 412, 686
BsmAI GTCTC 1 cut(s) 661
BsmBI CGTCTC 1 cut(s) 661
BsmFI GGGAC 1 cut(s) 861
BsmI GAATGC 1 cut(s) 449
BsnI GGCC 3 cut(s) 44, 380, 887
BsoBI CYCGRG 2 cut(s) 31, 749
Bsp143I GATC 5 cut(s) 544, 745, 1013, 1033, 1114
BspACI CCGC 3 cut(s) 593, 697, 920
BspANI GGCC 3 cut(s) 44, 380, 887
BspCNI CTCAG 4 cut(s) 60, 295, 766, 1118
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 4 cut(s) 747, 849, 985, 1035
BspMAI CTGCAG 2 cut(s) 664, 1195
BspMI ACCTGC 1 cut(s) 789
BspPI GGATC 4 cut(s) 740, 753, 1028, 1041
BsrI ACTGG 2 cut(s) 215, 1166
BssECI CCNNGG 4 cut(s) 31, 245, 750, 913
BssMI GATC 5 cut(s) 544, 745, 1013, 1033, 1114
Bst2UI CCWGG 2 cut(s) 247, 915
Bst4CI ACNGT 1 cut(s) 236
Bst6I CTCTTC 2 cut(s) 483, 756
BstC8I GCNNGC 5 cut(s) 168, 353, 357, 771, 1154
BstDEI CTNAG 4 cut(s) 47, 303, 774, 1105
BstF5I GGATG 4 cut(s) 141, 985, 1087, 1141
BstHHI GCGC 3 cut(s) 172, 254, 678
BstKTI GATC 5 cut(s) 547, 748, 1016, 1036, 1117
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 5 cut(s) 544, 745, 1013, 1033, 1114
BstMWI GCNNNNNNNGC 6 cut(s) 178, 893, 1064, 1193, 1199, 1202
BstNI CCWGG 2 cut(s) 247, 915
BstSCI CCNGG 5 cut(s) 30, 31, 92, 245, 913
BstSFI CTRYAG 2 cut(s) 660, 1191
BstV1I GCAGC 3 cut(s) 674, 1192, 1205
BstX2I RGATCY 2 cut(s) 745, 1033
BstXI CCANNNNNNTGG 1 cut(s) 443
BstYI RGATCY 2 cut(s) 745, 1033
Bsu36I CCTNAGG 1 cut(s) 1105
BsuRI GGCC 3 cut(s) 44, 380, 887
BtsCI GGATG 4 cut(s) 141, 985, 1087, 1141
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 5 cut(s) 168, 353, 357, 771, 1154
CaiI CAGNNNCTG 3 cut(s) 470, 534, 1208
CciI TCATGA 1 cut(s) 835
CfoI GCGC 3 cut(s) 172, 254, 678
Cfr13I GGNCC 6 cut(s) 43, 778, 791, 848, 917, 983
Cfr9I CCCGGG 1 cut(s) 31
Csp6I GTAC 2 cut(s) 599, 1182
CviAII CATG 9 cut(s) 154, 401, 610, 700, 808, 836, 883, 899, 1130
CviQI GTAC 2 cut(s) 599, 1182
DdeI CTNAG 4 cut(s) 47, 303, 774, 1105
DpnI GATC 5 cut(s) 546, 747, 1015, 1035, 1116
DpnII GATC 5 cut(s) 544, 745, 1013, 1033, 1114
EaeI YGGCCR 2 cut(s) 378, 885
Eam1104I CTCTTC 2 cut(s) 483, 756
EarI CTCTTC 2 cut(s) 483, 756
Eco32I GATATC 2 cut(s) 616, 1101
Eco47I GGWCC 5 cut(s) 778, 791, 848, 917, 983
Eco57I CTGAAG 3 cut(s) 102, 147, 969
Eco81I CCTNAGG 1 cut(s) 1105
Eco88I CYCGRG 2 cut(s) 31, 749
EcoO109I RGGNCCY 3 cut(s) 43, 778, 848
EcoRII CCWGG 2 cut(s) 245, 913
EcoRV GATATC 2 cut(s) 616, 1101
EcoT22I ATGCAT 1 cut(s) 1131
Esp3I CGTCTC 1 cut(s) 661
FaeI CATG 9 cut(s) 157, 404, 613, 703, 811, 839, 886, 902, 1133
FaqI GGGAC 1 cut(s) 861
FatI CATG 9 cut(s) 153, 400, 609, 699, 807, 835, 882, 898, 1129
Fnu4HI GCNGC 5 cut(s) 663, 697, 1194, 1203, 1206
FokI GGATG 4 cut(s) 148, 992, 1074, 1148
Fsp4HI GCNGC 5 cut(s) 663, 697, 1194, 1203, 1206
FspBI CTAG 2 cut(s) 260, 585
FspI TGCGCA 1 cut(s) 171
GlaI GCGC 3 cut(s) 171, 253, 677
GluI GCNGC 5 cut(s) 663, 697, 1194, 1203, 1206
GsuI CTGGAG 1 cut(s) 569
HaeIII GGCC 3 cut(s) 44, 380, 887
HapII CCGG 2 cut(s) 32, 93
HhaI GCGC 3 cut(s) 172, 254, 678
Hin1II CATG 9 cut(s) 157, 404, 613, 703, 811, 839, 886, 902, 1133
Hin6I GCGC 3 cut(s) 170, 252, 676
HinP1I GCGC 3 cut(s) 170, 252, 676
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 5 cut(s) 104, 366, 432, 467, 499
HpaII CCGG 2 cut(s) 32, 93
HphI GGTGA 3 cut(s) 720, 819, 1011
Hpy166II GTNNAC 1 cut(s) 991
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 8 cut(s) 101, 464, 548, 749, 836, 977, 1091, 1107
Hpy8I GTNNAC 1 cut(s) 991
HpyAV CCTTC 2 cut(s) 150, 178
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 2 cut(s) 118, 597
HpyCH4V TGCA 8 cut(s) 224, 449, 578, 662, 1010, 1129, 1171, 1193
HpyF10VI GCNNNNNNNGC 6 cut(s) 178, 893, 1064, 1193, 1199, 1202
HpyF3I CTNAG 4 cut(s) 47, 303, 774, 1105
HpySE526I ACGT 2 cut(s) 118, 597
Hsp92II CATG 9 cut(s) 157, 404, 613, 703, 811, 839, 886, 902, 1133
HspAI GCGC 3 cut(s) 170, 252, 676
Kzo9I GATC 5 cut(s) 544, 745, 1013, 1033, 1114
LmnI GCTCC 2 cut(s) 402, 913
Lsp1109I GCAGC 3 cut(s) 674, 1192, 1205
LweI GCATC 5 cut(s) 106, 126, 233, 316, 587
MaeI CTAG 2 cut(s) 260, 585
MaeII ACGT 2 cut(s) 118, 597
MaeIII GTNAC 1 cut(s) 8
MalI GATC 5 cut(s) 546, 747, 1015, 1035, 1116
MboI GATC 5 cut(s) 544, 745, 1013, 1033, 1114
MboII GAAGA 7 cut(s) 135, 140, 275, 441, 500, 773, 1137
MfeI CAATTG 1 cut(s) 360
MflI RGATCY 2 cut(s) 745, 1033
MlsI TGGCCA 2 cut(s) 380, 887
MluCI AATT 7 cut(s) 360, 540, 570, 713, 964, 1141, 1172
MluNI TGGCCA 2 cut(s) 380, 887
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 406
Mox20I TGGCCA 2 cut(s) 380, 887
Mph1103I ATGCAT 1 cut(s) 1131
MscI TGGCCA 2 cut(s) 380, 887
MseI TTAA 1 cut(s) 1176
MslI CAYNNNNRTG 1 cut(s) 1134
Msp20I TGGCCA 2 cut(s) 380, 887
MspA1I CMGCKG 2 cut(s) 665, 1205
MspI CCGG 2 cut(s) 32, 93
MspR9I CCNGG 5 cut(s) 32, 33, 94, 247, 915
MunI CAATTG 1 cut(s) 360
Mva1269I GAATGC 1 cut(s) 449
MvaI CCWGG 2 cut(s) 247, 915
MwoI GCNNNNNNNGC 6 cut(s) 178, 893, 1064, 1193, 1199, 1202
NciI CCSGG 3 cut(s) 32, 33, 94
NdeII GATC 5 cut(s) 544, 745, 1013, 1033, 1114
NlaIII CATG 9 cut(s) 157, 404, 613, 703, 811, 839, 886, 902, 1133
NlaIV GGNNCC 4 cut(s) 747, 849, 985, 1035
NmuCI GTSAC 1 cut(s) 8
NsbI TGCGCA 1 cut(s) 171
NsiI ATGCAT 1 cut(s) 1131
PagI TCATGA 1 cut(s) 835
PctI GAATGC 1 cut(s) 449
PfeI GAWTC 4 cut(s) 366, 432, 467, 499
PflMI CCANNNNNTGG 2 cut(s) 362, 686
PkrI GCNGC 5 cut(s) 664, 698, 1195, 1204, 1207
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PpuMI RGGWCCY 2 cut(s) 778, 848
Psp5II RGGWCCY 2 cut(s) 778, 848
Psp6I CCWGG 2 cut(s) 245, 913
PspGI CCWGG 2 cut(s) 245, 913
PspN4I GGNNCC 4 cut(s) 747, 849, 985, 1035
PspPI GGNCC 6 cut(s) 43, 778, 791, 848, 917, 983
PspPPI RGGWCCY 2 cut(s) 778, 848
PstI CTGCAG 2 cut(s) 664, 1195
PstNI CAGNNNCTG 3 cut(s) 470, 534, 1208
PsuI RGATCY 2 cut(s) 745, 1033
PvuII CAGCTG 2 cut(s) 665, 1205
RsaI GTAC 2 cut(s) 600, 1183
RsaNI GTAC 2 cut(s) 599, 1182
RseI CAYNNNNRTG 1 cut(s) 1134
SaqAI TTAA 1 cut(s) 1176
SatI GCNGC 5 cut(s) 663, 697, 1194, 1203, 1206
Sau3AI GATC 5 cut(s) 544, 745, 1013, 1033, 1114
Sau96I GGNCC 6 cut(s) 43, 778, 791, 848, 917, 983
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 5 cut(s) 32, 33, 94, 247, 915
SfaNI GCATC 5 cut(s) 106, 126, 233, 316, 587
SfcI CTRYAG 2 cut(s) 660, 1191
SinI GGWCC 5 cut(s) 778, 791, 848, 917, 983
SmaI CCCGGG 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 1134
Sse9I AATT 7 cut(s) 360, 540, 570, 713, 964, 1141, 1172
SsiI CCGC 3 cut(s) 593, 697, 920
SspMI CTAG 2 cut(s) 260, 585
StyD4I CCNGG 5 cut(s) 30, 31, 92, 245, 913
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 2 cut(s) 121, 600
TaqI TCGA 1 cut(s) 1117
TasI AATT 7 cut(s) 360, 540, 570, 713, 964, 1141, 1172
TauI GCSGC 1 cut(s) 699
TfiI GAWTC 4 cut(s) 366, 432, 467, 499
Tru1I TTAA 1 cut(s) 1176
Tru9I TTAA 1 cut(s) 1176
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 4 cut(s) 662, 1193, 1202, 1205
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 1 cut(s) 1076
TspGWI ACGGA 3 cut(s) 163, 300, 804
TspMI CCCGGG 1 cut(s) 31
Van91I CCANNNNNTGG 2 cut(s) 362, 686
VpaK11BI GGWCC 5 cut(s) 778, 791, 848, 917, 983
XmaI CCCGGG 1 cut(s) 31
XspI CTAG 2 cut(s) 260, 585
Zsp2I ATGCAT 1 cut(s) 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.