RchiOBHm_Chr2g0111411

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003589
Physical Location & Seq
Reverse (-)
23049227 .. 23049921
695 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 390 bp
ATGGTCACCAGCAGCTCTCGCGGTGGCACTTATACCACCATCAAGGAGCACATCACCTTCGACAAGGAGATCAAGAAGAGCAAATTCATTGTCCTCGCCGCTTCCGTCTCCGATGAACAATCCGCTCTCTCCATCCTCTCTCAGGTGGGAGATCAGTACCGGTCTAATGATGATGGTGAGCCATCTGGAACGACCGGGAAACCCATTCATTCTGCTATTGAGTCTTCCGGAATTGATAGAGTAATGGTACTGGTCATCCGGGAATATGGAGGAGTAACATCGGAATGCTTGAGAGATGCCCCAACTCATCCCGTAAAATCGAAGGTACTAACTTTCTTGCTCTGCTTTCGTGCTTTCATTTCAACTTGGGTGCCCAAAAATCTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

129

Amino Acids

14.09

Weight (kDa)

7.79

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 47 - 92 1.1e-10 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 370
AccBSI CCGCTC 1 cut(s) 125
AccII CGCG 1 cut(s) 21
AccIII TCCGGA 1 cut(s) 227
AciI CCGC 3 cut(s) 21, 99, 123
AcsI RAATTY 1 cut(s) 83
AfaI GTAC 3 cut(s) 158, 249, 327
AfiI CCNNNNNNNGG 1 cut(s) 142
AgeI ACCGGT 1 cut(s) 159
AgsI TTSAA 1 cut(s) 363
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 112
Aor13HI TCCGGA 1 cut(s) 227
ApeKI GCWGC 1 cut(s) 12
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
AsiGI ACCGGT 1 cut(s) 159
AsuC2I CCSGG 2 cut(s) 196, 260
AsuHPI GGTGA 2 cut(s) 46, 188
BaeGI GKGCMC 1 cut(s) 375
BanI GGYRCC 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 24
BccI CCATC 4 cut(s) 47, 140, 167, 190
BcnI CCSGG 2 cut(s) 196, 260
BcoDI GTCTC 1 cut(s) 112
BisI GCNGC 2 cut(s) 13, 99
BlsI GCNGC 2 cut(s) 14, 100
Bme1390I CCNGG 2 cut(s) 196, 260
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 2 cut(s) 196, 260
BmsI GCATC 1 cut(s) 286
BpiI GAAGAC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 310
BpuMI CCSGG 2 cut(s) 196, 260
BsaWI WCCGGW 2 cut(s) 159, 227
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse118I RCCGGY 1 cut(s) 159
Bse1I ACTGG 1 cut(s) 255
BseAI TCCGGA 1 cut(s) 227
BseGI GGATG 3 cut(s) 132, 255, 307
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 1 cut(s) 255
BseRI GAGGAG 1 cut(s) 285
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 1 cut(s) 24
Bsh1236I CGCG 1 cut(s) 21
Bsh1285I CGRYCG 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 370
BshTI ACCGGT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 4 cut(s) 160, 195, 228, 259
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 112
BsmBI CGTCTC 1 cut(s) 112
BsmI GAATGC 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 51, 375
Bsp13I TCCGGA 1 cut(s) 227
Bsp143I GATC 2 cut(s) 69, 151
BspACI CCGC 3 cut(s) 21, 99, 123
BspCNI CTCAG 1 cut(s) 154
BspEI TCCGGA 1 cut(s) 227
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 372
BspQI GCTCTTC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 370
BsrBI CCGCTC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 159
BsrI ACTGG 1 cut(s) 255
BssAI RCCGGY 1 cut(s) 159
BssMI GATC 2 cut(s) 69, 151
Bst6I CTCTTC 1 cut(s) 71
BstDEI CTNAG 1 cut(s) 141
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 3 cut(s) 132, 255, 307
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 2 cut(s) 72, 154
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 2 cut(s) 69, 151
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 1 cut(s) 18
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 2 cut(s) 194, 258
BstSLI GKGCMC 1 cut(s) 375
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 24
BstV2I GAAGAC 1 cut(s) 216
BtsCI GGATG 3 cut(s) 132, 255, 307
Cfr10I RCCGGY 1 cut(s) 159
Csp6I GTAC 3 cut(s) 157, 248, 326
CspAI ACCGGT 1 cut(s) 159
CviJI RGCY 2 cut(s) 15, 181
CviKI_1 RGCY 2 cut(s) 15, 181
CviQI GTAC 3 cut(s) 157, 248, 326
DdeI CTNAG 1 cut(s) 141
DpnI GATC 2 cut(s) 71, 153
DpnII GATC 2 cut(s) 69, 151
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco91I GGTNACC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 4
Esp3I CGTCTC 1 cut(s) 112
FaiI YATR 3 cut(s) 33, 267, 388
Fnu4HI GCNGC 2 cut(s) 13, 99
FokI GGATG 3 cut(s) 119, 242, 294
Fsp4HI GCNGC 2 cut(s) 13, 99
GluI GCNGC 2 cut(s) 13, 99
HapII CCGG 4 cut(s) 160, 195, 228, 259
HinfI GANTC 1 cut(s) 221
HpaII CCGG 4 cut(s) 160, 195, 228, 259
HphI GGTGA 2 cut(s) 46, 188
Hpy188I TCNGA 2 cut(s) 112, 283
Hpy188III TCNNGA 3 cut(s) 73, 186, 228
HpyAV CCTTC 2 cut(s) 67, 316
HpyF10VI GCNNNNNNNGC 1 cut(s) 18
HpyF3I CTNAG 1 cut(s) 141
Kpn2I TCCGGA 1 cut(s) 227
Kzo9I GATC 2 cut(s) 69, 151
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 8 cut(s) 22, 128, 171, 173, 208, 236, 241, 272
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 1 cut(s) 286
MaeIII GTNAC 2 cut(s) 4, 274
MalI GATC 2 cut(s) 71, 153
MbiI CCGCTC 1 cut(s) 125
MboI GATC 2 cut(s) 69, 151
MboII GAAGA 3 cut(s) 88, 216, 374
MhlI GDGCHC 2 cut(s) 51, 375
MluCI AATT 2 cut(s) 83, 231
MlyI GAGTC 1 cut(s) 230
MnlI CCTC 3 cut(s) 104, 146, 263
MroI TCCGGA 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 283
MspI CCGG 4 cut(s) 160, 195, 228, 259
MspR9I CCNGG 2 cut(s) 196, 260
Mva1269I GAATGC 1 cut(s) 290
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 1 cut(s) 18
NciI CCSGG 2 cut(s) 196, 260
NdeII GATC 2 cut(s) 69, 151
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 4
PciSI GCTCTTC 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 258
PinAI ACCGGT 1 cut(s) 159
PkrI GCNGC 2 cut(s) 14, 100
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PspEI GGTNACC 1 cut(s) 4
PspN4I GGNNCC 1 cut(s) 372
RsaI GTAC 3 cut(s) 158, 249, 327
RsaNI GTAC 3 cut(s) 157, 248, 326
RseI CAYNNNNRTG 1 cut(s) 283
SapI GCTCTTC 1 cut(s) 71
SatI GCNGC 2 cut(s) 13, 99
Sau3AI GATC 2 cut(s) 69, 151
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 196, 260
SduI GDGCHC 2 cut(s) 51, 375
SetI ASST 4 cut(s) 17, 59, 147, 327
SfaNI GCATC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
Sse9I AATT 2 cut(s) 83, 231
SsiI CCGC 3 cut(s) 21, 99, 123
StyD4I CCNGG 2 cut(s) 194, 258
TaqI TCGA 2 cut(s) 60, 320
TasI AATT 2 cut(s) 83, 231
TauI GCSGC 1 cut(s) 101
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 12
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 4 cut(s) 76, 129, 197, 346
TspGWI ACGGA 1 cut(s) 94
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.