RchiOBHm_Chr2g0153611

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005002
Physical Location & Seq
Reverse (-)
70932372 .. 70933333
962 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 387 bp
ATGTGTATCTTCACTTCTTCAGTTGTGAAGAGTTTAAAGTATGACAAGTCGCATGAGTGGGCGAGACACTTGGGTGGTATTATTATCCATGTTGAACTGCCTGATGTCGGTGAAGATGTAGCACAAGGCACCAGATGTGGTACTGTTGAGTCTGTCAAAGCCAGCAAGGGCCTCCACTCTACTCTCTCTGGCAATGTAGTTGAAGTAAATCAAAAGCTTCGTACCTCTCCTGCTTTGGTGAATCGAAGCCCGTATGAAGATGGATGGATCATGAAGGTAGAAATGAGCAACAAAGTGGAGCTGAATAATCTGATGGACTATGATGGGTACGCGAGCTATGTGAAAACCAAGTTGCCAAACATTGATGAACCAGTTGAAAACACTTAG

Protein Analysis

128

Amino Acids

14.21

Weight (kDa)

5.77

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 21 - 116 8.3e-29 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 128
AccII CGCG 1 cut(s) 332
AclWI GGATC 1 cut(s) 275
AfaI GTAC 3 cut(s) 142, 223, 329
AfiI CCNNNNNNNGG 1 cut(s) 107
AgsI TTSAA 3 cut(s) 95, 203, 377
AleI CACNNNNGTG 1 cut(s) 72
AluBI AGCT 3 cut(s) 217, 301, 336
AluI AGCT 3 cut(s) 217, 301, 336
Alw26I GTCTC 1 cut(s) 58
AlwI GGATC 1 cut(s) 275
AoxI GGCC 1 cut(s) 169
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 2 cut(s) 122, 250
BanI GGYRCC 1 cut(s) 128
BarI GAAGNNNNNNTAC 1 cut(s) 30
BccI CCATC 4 cut(s) 254, 258, 307, 317
BcoDI GTCTC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 1 cut(s) 130
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 371
Bse3DI GCAATG 1 cut(s) 199
BseGI GGATG 1 cut(s) 269
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMI GCAATG 1 cut(s) 199
BseNI ACTGG 1 cut(s) 371
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 1 cut(s) 171
BshNI GGYRCC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 107
BsmAI GTCTC 1 cut(s) 58
BsnI GGCC 1 cut(s) 171
Bsp143I GATC 1 cut(s) 267
BspANI GGCC 1 cut(s) 171
BspFNI CGCG 1 cut(s) 332
BspHI TCATGA 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 130
BspPI GGATC 1 cut(s) 275
BspT107I GGYRCC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 199
BsrI ACTGG 1 cut(s) 371
BssMI GATC 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 163, 334
BstDEI CTNAG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 269
BstFNI CGCG 1 cut(s) 332
BstKTI GATC 1 cut(s) 270
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 1 cut(s) 267
BstUI CGCG 1 cut(s) 332
BsuRI GGCC 1 cut(s) 171
BtsCI GGATG 1 cut(s) 269
Cac8I GCNNGC 2 cut(s) 163, 334
CciI TCATGA 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 169
Csp6I GTAC 3 cut(s) 141, 222, 328
CviAII CATG 3 cut(s) 53, 89, 271
CviJI RGCY 6 cut(s) 161, 171, 217, 249, 301, 336
CviKI_1 RGCY 6 cut(s) 161, 171, 217, 249, 301, 336
CviQI GTAC 3 cut(s) 141, 222, 328
DdeI CTNAG 1 cut(s) 384
DpnI GATC 1 cut(s) 269
DpnII GATC 1 cut(s) 267
DraI TTTAAA 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 169
FaeI CATG 3 cut(s) 56, 92, 274
FaiI YATR 7 cut(s) 42, 54, 90, 255, 272, 321, 339
FatI CATG 3 cut(s) 52, 88, 270
FokI GGATG 1 cut(s) 276
HaeIII GGCC 1 cut(s) 171
Hin1II CATG 3 cut(s) 56, 92, 274
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 2 cut(s) 149, 241
HphI GGTGA 2 cut(s) 122, 250
Hpy188I TCNGA 1 cut(s) 312
Hpy188III TCNNGA 1 cut(s) 271
HpyAV CCTTC 1 cut(s) 268
HpyCH4III ACNGT 1 cut(s) 145
HpyF3I CTNAG 1 cut(s) 384
Hsp92II CATG 3 cut(s) 56, 92, 274
Kzo9I GATC 1 cut(s) 267
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 5 cut(s) 114, 145, 174, 175, 243
MalI GATC 1 cut(s) 269
MboI GATC 1 cut(s) 267
MboII GAAGA 4 cut(s) 9, 40, 125, 269
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 2 cut(s) 182, 235
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 72
MvnI CGCG 1 cut(s) 332
NdeII GATC 1 cut(s) 267
NlaIII CATG 3 cut(s) 56, 92, 274
NlaIV GGNNCC 1 cut(s) 130
OliI CACNNNNGTG 1 cut(s) 72
PagI TCATGA 1 cut(s) 270
PfeI GAWTC 1 cut(s) 241
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 1 cut(s) 169
RsaI GTAC 3 cut(s) 142, 223, 329
RsaNI GTAC 3 cut(s) 141, 222, 328
RseI CAYNNNNRTG 1 cut(s) 72
SaqAI TTAA 1 cut(s) 35
Sau3AI GATC 1 cut(s) 267
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 1 cut(s) 158
SetI ASST 5 cut(s) 219, 227, 279, 303, 338
SmiMI CAYNNNNRTG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 145
TaqI TCGA 1 cut(s) 244
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TspDTI ATGAA 3 cut(s) 270, 287, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.