Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Reverse (-)

15731375 .. 15736691

Gene Structure 5317 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGACATCACCTCAGATACAAAACGACATCACCTCAGATACTCCTGGTCATGTCAATTTCTCTGCTGAAATGACTGCTTCACTTAGGCTCGCTGATGGTGCTGTCCTCATTGTTGATGCTGCAGAAGGGATGATGGTAAATACTGAGAGGGCTATACGCCTTGCAATCCAAGAGCGCCTACCAATTGTGGTTGTAATCAACAAGGTTGACAGGTTGATAACAGAACTTAAATTGCCCCCAAGGGATGCTTACTTCAAGCTTAGGCATACTCTGGAAGTGATTAATAACCACATAACGGCTGCCTCTTCAACTACTGGTCATGTACAAGTTATTGATCCTGTTGCTGGAAGTATGCTTTGCAAGCGCTACTGCAGGATGCTTCATGGGATTGCTTTTGACGCGGATCAGTTTGCTTCTCGTCTATGGGGAGATATGTACTATCATCCTGAGGACCTGGCTTTCAGGAGAAAACCCCCTGCAAGTGGAGGAGAAAGGTCGTTTGTCCAGTTTGTGCTTGAACCCCTCTATAAAATTTATAGCCAGGTGATTGGAGAACATAAAAAGAGTGTTGAGGCAACTCTTGCTGAACTTGGTGTCACCCTTAGCAATGCAGCTTACAAATTGAATGTTAGGCCTCTACTGAGGTTGGCTTGTAGCTCGGTTTTTGGATCTGCCTCGGGCTTCACTGATATGCTTGTGCAACATATTCCTTCTCCTAAAGATGCTGCAACAAGGAAAGTCGACCACACATATACTGGACCAAAAGACTCTTTGATTTACAAAGCCATGAAAACTTGTGATCCCAATGGCCCGTTGATGGTAAATGTTACCAAGCTGTATCCCAAGTCCGATTGTAGCGTGTTGGATGCATTTGGTAGGGTATACAGTGGCAAGATTCAGACAGGACAGTCAGTGCGAGTATTGGGAGAAGGGTATTCACCTGACGATGAGGAGGACATGACAGTCAAAGAAGTAATGAAGCTATGGCTTTACCAAGCGCGAGATAGGATACCTATAGCTGAAGCTCCTCCTGGTACTTGGGTTCTCATCGAAGGTGTGGATGCTTCCATAATGAAAACTGCCACTCTGTGTAATGAGAGTTATGACGAGGATTTATACATATTCCGTCCTCTTCAGTTCAATACACTGCCAGTGGTGAAAACGGCAACTGAGCCTCTAAATCCCAGTGAGCTGCCAAAAATGGTGGAGGGTCTTAGAAAGACCAGCATGAGTTATCCTCTTGCCATAACCAAAGTTGAGGAGTCTGGGGAGCACACAATCTTAGGTACAGGAGAACTGTACTTGGATTCCATCATGATGGACCTCAGAGAGCTCTACTCTGAAGTAGAAGTCAAGGTTGCAGATCCTGTTGTTTCATTCTGCGAAACAGTTGTGGAATCTTCATCAATGAAGTGTTTTGCTGAAACACCAAACAAGAAGAATAAAACTATGATTGCAGAGCCATTAGAGAGAGGACTTGCGGAGGACATTGAGAAAGGTGTTGTAAGCATTGATTGGTCTAGGAGAGATATAGGTAATTTCTTCCAGACTAGGTATGAGTGCGATGTTCTTGCTGCACGGTCCATATGGGCATTCGGTCCCGGTCCATATGGGCATTCGGGACCCAACATACTACTTGATGACACACTTTCAAGCGAAGTTGACAAGAGCATGCTGAATGCTGTGAAAGACTCCATTCTGCAAGGAAATGTTAAGTTCAAGATTGTTGTTGCAAGGATTGCACCTGAGCCGTTGCATCGTGGATCTGGTCAAATCATTCCCACATCTCGATGTGTGGCCTATTCGTCTTTCCTAATGGCAACCCCACGGCTCATGGAGCCAGTATGTTATGTGGAGATACAAATGCCAATCGACTGTATATCTGCAATCTACACTGTTTTATCTCGCAGGCGTGGACATGTTACAGCCGATGTTCCTCAACCCGGGACCCCAGCTTACATCGTTAAGGCATTTTTACCCGTCATAGAATCGTTTGGTTTTGAGACGGACTTGAGGTACCATACTCAAGGACAGGCTTTTTGCCTCTCAGTCTTTGATCATTGGGCTATAGTTCCTGGGGATCCTCTTGACAAGAGTATTGTTTTGCCACCACTTGAACCCGCACCGATTCAGCACCTGGCTCGTGAATTTATGGTCAAGACAAGGCGTAGAAAGGGAATGAGCGAGGATGTGAGCATCAGCAAATTTTTTGATGAGGCCATGATGGTGGAACTGGCTCAGCAGGAAGCTGATATTCACCAGCAAATGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

757

Amino Acids

84.22

Weight (kDa)

5.63

Isoelectric Point (pI)

40.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	13 - 114	1.6e-23	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	290 - 364	3e-09	Elongation factor Tu domain 2
EFG_C	PF00679	610 - 698	1.4e-21	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	907, 962
Acc65I	GGTACC	1 cut(s)	2016
AccB1I	GGYRCC	1 cut(s)	2016
AccI	GTMKAC	2 cut(s)	741, 882
AccII	CGCG	2 cut(s)	401, 1000
AciI	CCGC	3 cut(s)	401, 1481, 2121
AclWI	GGATC	8 cut(s)	329, 411, 676, 794, 1358, 1771, 2075, 2088
AcsI	RAATTY	3 cut(s)	531, 2147, 2204
AcuI	CTGAAG	3 cut(s)	1041, 1118, 1362
AdeI	CACNNNGTG	1 cut(s)	1089
AfaI	GTAC	6 cut(s)	324, 437, 1036, 1288, 1301, 2018
AfeI	AGCGCT	1 cut(s)	365
AfiI	CCNNNNNNNGG	5 cut(s)	295, 344, 482, 817, 1943
AflIII	ACRYGT	1 cut(s)	1918
AgsI	TTSAA	8 cut(s)	256, 309, 518, 625, 1141, 1653, 1720, 2117
AjnI	CCWGG	6 cut(s)	43, 453, 540, 1030, 2074, 2136
Alw21I	GWGCWC	2 cut(s)	1275, 1335
Alw26I	GTCTC	1 cut(s)	1997
AlwI	GGATC	8 cut(s)	329, 411, 676, 794, 1358, 1771, 2075, 2088
AlwNI	CAGNNNCTG	2 cut(s)	1367, 2137
Ama87I	CYCGRG	2 cut(s)	676, 1943
Aor51HI	AGCGCT	1 cut(s)	365
AoxI	GGCC	4 cut(s)	632, 808, 1797, 2217
ApeKI	GCWGC	6 cut(s)	119, 299, 611, 725, 1192, 1574
ApoI	RAATTY	3 cut(s)	531, 2147, 2204
ArsI	GACNNNNNNTTYG	2 cut(s)	2022, 2054
AseI	ATTAAT	1 cut(s)	282
Asp718I	GGTACC	1 cut(s)	2016
AspLEI	GCGC	3 cut(s)	177, 366, 1000
AspS9I	GGNCC	9 cut(s)	451, 758, 809, 1321, 1581, 1598, 1604, 1622, 1947
AsuC2I	CCSGG	3 cut(s)	1602, 1944, 1945
AsuHPI	GGTGA	6 cut(s)	22, 556, 589, 930, 1168, 2249
AvaI	CYCGRG	2 cut(s)	676, 1943
AvaII	GGWCC	8 cut(s)	451, 758, 1321, 1581, 1598, 1604, 1622, 1947
AxyI	CCTNAGG	1 cut(s)	447
BaeI	ACNNNNGTAYC	2 cut(s)	1026, 1059
BamHI	GGATCC	1 cut(s)	2080
BanI	GGYRCC	1 cut(s)	2016
BanII	GRGCYC	1 cut(s)	1335
BauI	CACGAG	1 cut(s)	2142
Bbv12I	GWGCWC	2 cut(s)	1275, 1335
BbvI	GCAGC	6 cut(s)	106, 286, 623, 712, 1179, 1561
BccI	CCATC	6 cut(s)	89, 127, 811, 1312, 1319, 2218
BceAI	ACGGC	4 cut(s)	312, 1179, 1735, 1844
BcgI	CGANNNNNNTGC	4 cut(s)	1553, 1587, 2123, 2157
BciT130I	CCWGG	6 cut(s)	45, 455, 542, 1032, 2076, 2138
BciVI	GTATCC	2 cut(s)	849, 1002
BclI	TGATCA	1 cut(s)	2056
BcnI	CCSGG	3 cut(s)	1602, 1944, 1945
BcoDI	GTCTC	1 cut(s)	1997
BfaI	CTAG	2 cut(s)	1521, 1551
BfmI	CTRYAG	4 cut(s)	120, 370, 1014, 2067
BfoI	RGCGCY	2 cut(s)	178, 367
BfuI	GTATCC	2 cut(s)	849, 1002
BisI	GCNGC	6 cut(s)	120, 300, 612, 726, 1193, 1575
BlpI	GCTNAGC	1 cut(s)	2238
BlsI	GCNGC	6 cut(s)	121, 301, 613, 727, 1194, 1576
Bme1390I	CCNGG	9 cut(s)	45, 455, 542, 1032, 1602, 1944, 1945, 2076, 2138
Bme18I	GGWCC	8 cut(s)	451, 758, 1321, 1581, 1598, 1604, 1622, 1947
BmeT110I	CYCGRG	2 cut(s)	676, 1943
BmgT120I	GGNCC	9 cut(s)	451, 758, 809, 1321, 1581, 1598, 1604, 1622, 1947
BmiI	GGNNCC	8 cut(s)	1600, 1623, 1624, 1839, 1948, 1949, 2018, 2082
BmrFI	CCNGG	9 cut(s)	45, 455, 542, 1032, 1602, 1944, 1945, 2076, 2138
BmrI	ACTGGG	1 cut(s)	1179
BmsI	GCATC	8 cut(s)	106, 235, 366, 712, 856, 1051, 1765, 2205
BmuI	ACTGGG	1 cut(s)	1179
BplI	GAGNNNNNCTC	4 cut(s)	1222, 1254, 1322, 1354
Bpu10I	CCTNAGC	3 cut(s)	260, 602, 1746
Bpu1102I	GCTNAGC	1 cut(s)	2238
BpuEI	CTTGAG	2 cut(s)	2010, 2032
BpuMI	CCSGG	3 cut(s)	1602, 1944, 1945
BsaJI	CCNNGG	5 cut(s)	239, 675, 1826, 1943, 2075
Bsc4I	CCNNNNNNNGG	5 cut(s)	295, 344, 482, 817, 1943
Bse1I	ACTGG	7 cut(s)	319, 505, 760, 1151, 1185, 1841, 2238
Bse21I	CCTNAGG	1 cut(s)	447
Bse3DI	GCAATG	1 cut(s)	613
BseBI	CCWGG	6 cut(s)	45, 455, 542, 1032, 2076, 2138
BseDI	CCNNGG	5 cut(s)	239, 675, 1826, 1943, 2075
BseGI	GGATG	7 cut(s)	135, 250, 381, 442, 871, 1066, 2194
BseLI	CCNNNNNNNGG	5 cut(s)	295, 344, 482, 817, 1943
BseMI	GCAATG	1 cut(s)	613
BseNI	ACTGG	7 cut(s)	319, 505, 760, 1151, 1185, 1841, 2238
BseRI	GAGGAG	4 cut(s)	501, 965, 1017, 1274
BseXI	GCAGC	6 cut(s)	106, 286, 623, 712, 1179, 1561
BseYI	CCCAGC	1 cut(s)	1951
BsgI	GTGCAG	1 cut(s)	1560
Bsh1236I	CGCG	2 cut(s)	401, 1000
BshFI	GGCC	4 cut(s)	634, 810, 1799, 2219
BshNI	GGYRCC	1 cut(s)	2016
BsiHKAI	GWGCWC	2 cut(s)	1275, 1335
BsiHKCI	CYCGRG	2 cut(s)	676, 1943
BsiSI	CCGG	2 cut(s)	1602, 1944
BslFI	GGGAC	3 cut(s)	1584, 1635, 1960
BslI	CCNNNNNNNGG	5 cut(s)	295, 344, 482, 817, 1943
BsmAI	GTCTC	1 cut(s)	1997
BsmBI	CGTCTC	1 cut(s)	1997
BsmFI	GGGAC	3 cut(s)	1584, 1635, 1960
BsmI	GAATGC	3 cut(s)	1592, 1615, 1684
BsnI	GGCC	4 cut(s)	634, 810, 1799, 2219
BsoBI	CYCGRG	2 cut(s)	676, 1943
Bsp1286I	GDGCHC	2 cut(s)	1275, 1335
Bsp1407I	TGTACA	1 cut(s)	322
Bsp143I	GATC	8 cut(s)	334, 403, 668, 799, 1363, 1763, 2056, 2080
Bsp1720I	GCTNAGC	1 cut(s)	2238
BspACI	CCGC	3 cut(s)	401, 1481, 2121
BspANI	GGCC	4 cut(s)	634, 810, 1799, 2219
BspFNI	CGCG	2 cut(s)	401, 1000
BspHI	TCATGA	1 cut(s)	1314
BspLI	GGNNCC	8 cut(s)	1600, 1623, 1624, 1839, 1948, 1949, 2018, 2082
BspMAI	CTGCAG	2 cut(s)	124, 374
BspPI	GGATC	8 cut(s)	329, 411, 676, 794, 1358, 1771, 2075, 2088
BspT107I	GGYRCC	1 cut(s)	2016
BsrDI	GCAATG	1 cut(s)	613
BsrGI	TGTACA	1 cut(s)	322
BsrI	ACTGG	7 cut(s)	319, 505, 760, 1151, 1185, 1841, 2238
BssECI	CCNNGG	5 cut(s)	239, 675, 1826, 1943, 2075
BssMI	GATC	8 cut(s)	334, 403, 668, 799, 1363, 1763, 2056, 2080
BssNAI	GTATAC	1 cut(s)	883
BssSI	CACGAG	1 cut(s)	2142
BssT1I	CCWWGG	1 cut(s)	239
Bst1107I	GTATAC	1 cut(s)	883
Bst2BI	CACGAG	1 cut(s)	2142
Bst2UI	CCWGG	6 cut(s)	45, 455, 542, 1032, 2076, 2138
Bst4CI	ACNGT	8 cut(s)	887, 909, 964, 1299, 1390, 1581, 1877, 1897
Bst6I	CTCTTC	2 cut(s)	310, 1137
BstAPI	GCANNNNNTGC	2 cut(s)	581, 1739
BstAUI	TGTACA	1 cut(s)	322
BstC8I	GCNNGC	4 cut(s)	90, 362, 1673, 1910
BstDSI	CCRYGG	1 cut(s)	1826
BstF5I	GGATG	7 cut(s)	135, 250, 381, 442, 871, 1066, 2194
BstFNI	CGCG	2 cut(s)	401, 1000
BstH2I	RGCGCY	2 cut(s)	178, 367
BstHHI	GCGC	3 cut(s)	177, 366, 1000
BstKTI	GATC	8 cut(s)	337, 406, 671, 802, 1366, 1766, 2059, 2083
BstMAI	GTCTC	1 cut(s)	1997
BstMBI	GATC	8 cut(s)	334, 403, 668, 799, 1363, 1763, 2056, 2080
BstMWI	GCNNNNNNNGC	6 cut(s)	98, 361, 398, 581, 1739, 1837
BstNI	CCWGG	6 cut(s)	45, 455, 542, 1032, 2076, 2138
BstNSI	RCATGY	2 cut(s)	1675, 1922
BstSCI	CCNGG	9 cut(s)	43, 453, 540, 1030, 1600, 1942, 1943, 2074, 2136
BstSFI	CTRYAG	4 cut(s)	120, 370, 1014, 2067
BstUI	CGCG	2 cut(s)	401, 1000
BstV1I	GCAGC	6 cut(s)	106, 286, 623, 712, 1179, 1561
BstX2I	RGATCY	4 cut(s)	668, 1363, 1763, 2080
BstXI	CCANNNNNNTGG	3 cut(s)	548, 1318, 2227
BstYI	RGATCY	4 cut(s)	668, 1363, 1763, 2080
BstZ17I	GTATAC	1 cut(s)	883
Bsu36I	CCTNAGG	1 cut(s)	447
BsuI	GTATCC	2 cut(s)	849, 1002
BsuRI	GGCC	4 cut(s)	634, 810, 1799, 2219
BtgI	CCRYGG	1 cut(s)	1826
BtgZI	GCGATG	1 cut(s)	1578
BtsCI	GGATG	7 cut(s)	135, 250, 381, 442, 871, 1066, 2194
BtsI	GCAGTG	1 cut(s)	1145
BtsIMutI	CAGTG	7 cut(s)	684, 892, 918, 1145, 1158, 1192, 1893
Cac8I	GCNNGC	4 cut(s)	90, 362, 1673, 1910
CaiI	CAGNNNCTG	2 cut(s)	1367, 2137
CciI	TCATGA	1 cut(s)	1314
CfoI	GCGC	3 cut(s)	177, 366, 1000
Cfr13I	GGNCC	9 cut(s)	451, 758, 809, 1321, 1581, 1598, 1604, 1622, 1947
Cfr9I	CCCGGG	1 cut(s)	1943
CseI	GACGC	1 cut(s)	407
Csp6I	GTAC	6 cut(s)	323, 436, 1035, 1287, 1300, 2017
CspCI	CAANNNNNGTGG	2 cut(s)	1185, 1220
CviQI	GTAC	6 cut(s)	323, 436, 1035, 1287, 1300, 2017
DpnI	GATC	8 cut(s)	336, 405, 670, 801, 1365, 1765, 2058, 2082
DpnII	GATC	8 cut(s)	334, 403, 668, 799, 1363, 1763, 2056, 2080
DraIII	CACNNNGTG	1 cut(s)	1089
DrdI	GACNNNNNNGTC	2 cut(s)	907, 962
DseDI	GACNNNNNNGTC	2 cut(s)	907, 962
Eam1104I	CTCTTC	2 cut(s)	310, 1137
EarI	CTCTTC	2 cut(s)	310, 1137
Ecl136II	GAGCTC	1 cut(s)	1333
Eco130I	CCWWGG	1 cut(s)	239
Eco147I	AGGCCT	1 cut(s)	634
Eco24I	GRGCYC	1 cut(s)	1335
Eco47I	GGWCC	8 cut(s)	451, 758, 1321, 1581, 1598, 1604, 1622, 1947
Eco47III	AGCGCT	1 cut(s)	365
Eco53kI	GAGCTC	1 cut(s)	1333
Eco57I	CTGAAG	3 cut(s)	1041, 1118, 1362
Eco81I	CCTNAGG	1 cut(s)	447
Eco88I	CYCGRG	2 cut(s)	676, 1943
EcoICRI	GAGCTC	1 cut(s)	1333
EcoO109I	RGGNCCY	3 cut(s)	451, 1622, 1947
EcoRII	CCWGG	6 cut(s)	43, 453, 540, 1030, 2074, 2136
EcoT14I	CCWWGG	1 cut(s)	239
EcoT22I	ATGCAT	1 cut(s)	871
EcoT38I	GRGCYC	1 cut(s)	1335
ErhI	CCWWGG	1 cut(s)	239
Esp3I	CGTCTC	1 cut(s)	1997
FalI	AAGNNNNNCTT	2 cut(s)	232, 264
FaqI	GGGAC	3 cut(s)	1584, 1635, 1960
FauI	CCCGC	1 cut(s)	2128
FauNDI	CATATG	2 cut(s)	1586, 1609
FbaI	TGATCA	1 cut(s)	2056
FblI	GTMKAC	2 cut(s)	741, 882
Fnu4HI	GCNGC	6 cut(s)	120, 300, 612, 726, 1193, 1575
FokI	GGATG	7 cut(s)	142, 257, 388, 429, 878, 1073, 2201
FriOI	GRGCYC	1 cut(s)	1335
Fsp4HI	GCNGC	6 cut(s)	120, 300, 612, 726, 1193, 1575
FspBI	CTAG	2 cut(s)	1521, 1551
GlaI	GCGC	3 cut(s)	176, 365, 999
GluI	GCNGC	6 cut(s)	120, 300, 612, 726, 1193, 1575
GsaI	CCCAGC	1 cut(s)	1955
HaeII	RGCGCY	2 cut(s)	178, 367
HaeIII	GGCC	4 cut(s)	634, 810, 1799, 2219
HapII	CCGG	2 cut(s)	1602, 1944
HgaI	GACGC	1 cut(s)	407
HhaI	GCGC	3 cut(s)	177, 366, 1000
Hin6I	GCGC	3 cut(s)	175, 364, 998
HinP1I	GCGC	3 cut(s)	175, 364, 998
HincII	GTYRAC	3 cut(s)	208, 742, 1663
HindII	GTYRAC	3 cut(s)	208, 742, 1663
HindIII	AAGCTT	1 cut(s)	257
HinfI	GANTC	8 cut(s)	767, 895, 1262, 1307, 1397, 1691, 1988, 2128
HpaII	CCGG	2 cut(s)	1602, 1944
HphI	GGTGA	6 cut(s)	22, 556, 589, 930, 1168, 2249
Hpy166II	GTNNAC	5 cut(s)	208, 742, 883, 1663, 1916
Hpy188I	TCNGA	6 cut(s)	15, 37, 850, 900, 1328, 1342
Hpy8I	GTNNAC	5 cut(s)	208, 742, 883, 1663, 1916
HpyAV	CCTTC	4 cut(s)	119, 720, 923, 1046
HpyCH4III	ACNGT	8 cut(s)	887, 909, 964, 1299, 1390, 1581, 1877, 1897
HpyF10VI	GCNNNNNNNGC	6 cut(s)	98, 361, 398, 581, 1739, 1837
HspAI	GCGC	3 cut(s)	175, 364, 998
KflI	GGGWCCC	2 cut(s)	1622, 1947
KpnI	GGTACC	1 cut(s)	2020
Ksp22I	TGATCA	1 cut(s)	2056
Kzo9I	GATC	8 cut(s)	334, 403, 668, 799, 1363, 1763, 2056, 2080
LmnI	GCTCC	3 cut(s)	1030, 1270, 1837
Lsp1109I	GCAGC	6 cut(s)	106, 286, 623, 712, 1179, 1561
LweI	GCATC	8 cut(s)	106, 235, 366, 712, 856, 1051, 1765, 2205
MaeI	CTAG	2 cut(s)	1521, 1551
MaeIII	GTNAC	3 cut(s)	595, 826, 1921
MalI	GATC	8 cut(s)	336, 405, 670, 801, 1365, 1765, 2058, 2082
MboI	GATC	8 cut(s)	334, 403, 668, 799, 1363, 1763, 2056, 2080
MboII	GAAGA	5 cut(s)	297, 1124, 1392, 1450, 1534
MfeI	CAATTG	1 cut(s)	183
MflI	RGATCY	4 cut(s)	668, 1363, 1763, 2080
MhlI	GDGCHC	2 cut(s)	1275, 1335
MluCI	AATT	8 cut(s)	55, 183, 230, 531, 620, 1537, 2147, 2204
MlyI	GAGTC	3 cut(s)	761, 1271, 1685
MmeI	TCCRAC	1 cut(s)	843
Mph1103I	ATGCAT	1 cut(s)	871
MseI	TTAA	4 cut(s)	228, 282, 1713, 1965
MslI	CAYNNNNRTG	4 cut(s)	689, 1316, 1789, 2225
MspI	CCGG	2 cut(s)	1602, 1944
MspR9I	CCNGG	9 cut(s)	45, 455, 542, 1032, 1602, 1944, 1945, 2076, 2138
MunI	CAATTG	1 cut(s)	183
Mva1269I	GAATGC	3 cut(s)	1592, 1615, 1684
MvaI	CCWGG	6 cut(s)	45, 455, 542, 1032, 2076, 2138
MvnI	CGCG	2 cut(s)	401, 1000
MwoI	GCNNNNNNNGC	6 cut(s)	98, 361, 398, 581, 1739, 1837
NciI	CCSGG	3 cut(s)	1602, 1944, 1945
NdeI	CATATG	2 cut(s)	1586, 1609
NdeII	GATC	8 cut(s)	334, 403, 668, 799, 1363, 1763, 2056, 2080
NlaIV	GGNNCC	8 cut(s)	1600, 1623, 1624, 1839, 1948, 1949, 2018, 2082
NmuCI	GTSAC	1 cut(s)	595
NsiI	ATGCAT	1 cut(s)	871
NspI	RCATGY	2 cut(s)	1675, 1922
PaeI	GCATGC	1 cut(s)	1675
PagI	TCATGA	1 cut(s)	1314
PceI	AGGCCT	1 cut(s)	634
PciI	ACATGT	1 cut(s)	1918
PctI	GAATGC	3 cut(s)	1592, 1615, 1684
PfeI	GAWTC	5 cut(s)	895, 1307, 1397, 1988, 2128
PkrI	GCNGC	6 cut(s)	121, 301, 613, 727, 1194, 1576
PleI	GAGTC	3 cut(s)	761, 1270, 1685
PpsI	GAGTC	3 cut(s)	761, 1270, 1685
PpuMI	RGGWCCY	3 cut(s)	451, 1622, 1947
PscI	ACATGT	1 cut(s)	1918
PshBI	ATTAAT	1 cut(s)	282
Psp124BI	GAGCTC	1 cut(s)	1335
Psp5II	RGGWCCY	3 cut(s)	451, 1622, 1947
Psp6I	CCWGG	6 cut(s)	43, 453, 540, 1030, 2074, 2136
PspFI	CCCAGC	1 cut(s)	1951
PspGI	CCWGG	6 cut(s)	43, 453, 540, 1030, 2074, 2136
PspN4I	GGNNCC	8 cut(s)	1600, 1623, 1624, 1839, 1948, 1949, 2018, 2082
PspPI	GGNCC	9 cut(s)	451, 758, 809, 1321, 1581, 1598, 1604, 1622, 1947
PspPPI	RGGWCCY	3 cut(s)	451, 1622, 1947
PstI	CTGCAG	2 cut(s)	124, 374
PstNI	CAGNNNCTG	2 cut(s)	1367, 2137
PsuI	RGATCY	4 cut(s)	668, 1363, 1763, 2080
RsaI	GTAC	6 cut(s)	324, 437, 1036, 1288, 1301, 2018
RsaNI	GTAC	6 cut(s)	323, 436, 1035, 1287, 1300, 2017
RseI	CAYNNNNRTG	4 cut(s)	689, 1316, 1789, 2225
SacI	GAGCTC	1 cut(s)	1335
SalI	GTCGAC	1 cut(s)	740
SaqAI	TTAA	4 cut(s)	228, 282, 1713, 1965
SatI	GCNGC	6 cut(s)	120, 300, 612, 726, 1193, 1575
Sau3AI	GATC	8 cut(s)	334, 403, 668, 799, 1363, 1763, 2056, 2080
Sau96I	GGNCC	9 cut(s)	451, 758, 809, 1321, 1581, 1598, 1604, 1622, 1947
SchI	GAGTC	3 cut(s)	761, 1271, 1685
ScrFI	CCNGG	9 cut(s)	45, 455, 542, 1032, 1602, 1944, 1945, 2076, 2138
SduI	GDGCHC	2 cut(s)	1275, 1335
SfaNI	GCATC	8 cut(s)	106, 235, 366, 712, 856, 1051, 1765, 2205
SfcI	CTRYAG	4 cut(s)	120, 370, 1014, 2067
SinI	GGWCC	8 cut(s)	451, 758, 1321, 1581, 1598, 1604, 1622, 1947
SmaI	CCCGGG	1 cut(s)	1945
SmiMI	CAYNNNNRTG	4 cut(s)	689, 1316, 1789, 2225
SmlI	CTYRAG	2 cut(s)	2011, 2025
SmoI	CTYRAG	2 cut(s)	2011, 2025
SphI	GCATGC	1 cut(s)	1675
Sse9I	AATT	8 cut(s)	55, 183, 230, 531, 620, 1537, 2147, 2204
SseBI	AGGCCT	1 cut(s)	634
SsiI	CCGC	3 cut(s)	401, 1481, 2121
SspMI	CTAG	2 cut(s)	1521, 1551
SstI	GAGCTC	1 cut(s)	1335
StuI	AGGCCT	1 cut(s)	634
StyD4I	CCNGG	9 cut(s)	43, 453, 540, 1030, 1600, 1942, 1943, 2074, 2136
StyI	CCWWGG	1 cut(s)	239
TaaI	ACNGT	8 cut(s)	887, 909, 964, 1299, 1390, 1581, 1877, 1897
TaqI	TCGA	4 cut(s)	741, 1050, 1789, 1872
TaqII	GACCGA	1 cut(s)	1586
TasI	AATT	8 cut(s)	55, 183, 230, 531, 620, 1537, 2147, 2204
TatI	WGTACW	3 cut(s)	322, 435, 1299
TfiI	GAWTC	5 cut(s)	895, 1307, 1397, 1988, 2128
Tru1I	TTAA	4 cut(s)	228, 282, 1713, 1965
Tru9I	TTAA	4 cut(s)	228, 282, 1713, 1965
TscAI	CASTG	7 cut(s)	691, 892, 918, 1152, 1158, 1192, 1900
TseFI	GTSAC	1 cut(s)	595
TseI	GCWGC	6 cut(s)	119, 299, 611, 725, 1192, 1574
Tsp45I	GTSAC	1 cut(s)	595
TspDTI	ATGAA	7 cut(s)	371, 803, 992, 1088, 1365, 1392, 1424
TspGWI	ACGGA	2 cut(s)	1115, 2021
TspMI	CCCGGG	1 cut(s)	1943
TspRI	CASTG	7 cut(s)	691, 892, 918, 1152, 1158, 1192, 1900
VpaK11BI	GGWCC	8 cut(s)	451, 758, 1321, 1581, 1598, 1604, 1622, 1947
VspI	ATTAAT	1 cut(s)	282
XapI	RAATTY	3 cut(s)	531, 2147, 2204
XceI	RCATGY	2 cut(s)	1675, 1922
XcmI	CCANNNNNNNNNTGG	1 cut(s)	752
XmaI	CCCGGG	1 cut(s)	1943
XmiI	GTMKAC	2 cut(s)	741, 882
XspI	CTAG	2 cut(s)	1521, 1551
Zsp2I	ATGCAT	1 cut(s)	871

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.