RchiOBHm_Chr1g0354081

tRNA-specific adenosine deaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005289
Physical Location & Seq
Forward (+)
47221657 .. 47225933
4277 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1314 bp
ATGACGTCGCCTCGCTGCTTCGAAACGCCGTCGTTTCGTCCTTCTTCTCCAGACTCAGACTGGGGACACAAAGTCTCAGACAAGGTGCTCTCAGTCTACAACTCGCTCCCTAAGAAAGGGAAGCCCCAGGGCCGCGAAGTCACCGTCTTGGCCGCCTTCCTCCTCTCCTCTCCTCTTCAAGGTCCTCTCTTTTCCTTTTTCTCTTCCGCTTTTATTGAAACCAAACACTCTCTCTGCATCATTGATTTTGAATTGCTGATCAGAATTGCAAGTTGTGGCATTGGGAACCGGAACCAAATGCGTCGGCCGTTCACTCCTCAGCCCCCACGGCGATATCGTCAACGATTCCCACGCCGAGGTCATCGCTCGCCGCGCTTTAATCAGATACAATGTCTTTCTCAAATTTATAGTAGGCAAAGAGATAACAATGGAAGCAAACAGTTGCGAAGCGATGATGTGAAAAAGTTTATTTTTGAATTGGACCCTGATGATGGGCACGGAAAATACACAATGAGGAAGGGTTGGAAATTGCACATGTATATATCGCAACTACCTTGTGGGATTGCTTCACCAAGTTCATTGATGTCTCCCCCGAAAAATATCTCACCTAGCGGAGGGGTTCATTACTGGAGGAGCTTAATGAGTGATGCTTCACAACTAATTGGCACGGTTCAAAGAAAGCCTGGTCGTGGTGATACTACATTATCAGTTAGCTGCTCTGACAAGATAGCCCGTTGGAATGTTGTTGGAGTTCAAGGAGCTTTGCTTTCCTTCTTTCTTCAACCTGTATACCTTTCTTCCATTACTGTTGGACAATCCCCTATCAACTCCGAAAAGGTTCTTGTGGCTGATTGTCTGAAACAAGCTTTGCATGATCGTGTCCTACCATTATCAAGCGAGCTGATCAGTCCCTTTCAGCCACTACTCTGGGAAGCTCCAATGCCACCAAAGGAATTTCAACATTCTGAGACCGCTTTGATCACTATAACATGCGGGTATTCAATATGTTGGAATAAGTCTGGTTTGCATGAAGTCATTCTTGGAACCATAGGAAGAAAGCAAGGCGCTGCAAAAGGAGCACGCAGTCCGTCTACTGAATCCCCTATATGCAAAAAACGGCTTTTGGAGATTTTCTTGTCCCTAAGGAATGAATGCCCCACCAAAATCCATGTCAATGAGATGTCGTATCGAGAAATTAAGGAAAGGGCACATGAATACAATGCAATGTCAAAAGTTCTAAAAAGAAGACATACCTTTAGTAATTGGCTATTGAAGCCCCCAGATTTGGAGGCATTCTCTGCTATGGGAGAGTGA

Protein Analysis

437

Amino Acids

49.28

Weight (kDa)

9.74

Isoelectric Point (pI)

62.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_deamin PF02137 152 - 426 7.5e-54 Adenosine-deaminase (editase) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccI GTMKAC 3 cut(s) 96, 789, 1091
AccII CGCG 2 cut(s) 135, 373
AciI CCGC 7 cut(s) 133, 153, 207, 371, 612, 972, 993
AcoI YGGCCR 2 cut(s) 150, 305
AcsI RAATTY 2 cut(s) 402, 953
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 7 cut(s) 116, 179, 356, 491, 614, 689, 1285
AflIII ACRYGT 1 cut(s) 534
AhdI GACNNNNNGTC 1 cut(s) 71
AjnI CCWGG 2 cut(s) 126, 682
AjuI GAANNNNNNNTTGG 2 cut(s) 1023, 1055
AluBI AGCT 6 cut(s) 636, 714, 761, 866, 901, 935
AluI AGCT 6 cut(s) 636, 714, 761, 866, 901, 935
Alw21I GWGCWC 2 cut(s) 90, 1081
Alw26I GTCTC 3 cut(s) 79, 591, 962
AoxI GGCC 3 cut(s) 130, 150, 305
ApeKI GCWGC 3 cut(s) 15, 714, 1067
ApoI RAATTY 2 cut(s) 402, 953
ArsI GACNNNNNNTTYG 4 cut(s) 129, 161, 1155, 1187
Asp700I GAANNNNTTC 2 cut(s) 837, 1035
AspLEI GCGC 2 cut(s) 375, 1067
AspS9I GGNCC 3 cut(s) 130, 182, 481
AsuHPI GGTGA 4 cut(s) 133, 561, 597, 704
AsuII TTCGAA 1 cut(s) 21
AvaII GGWCC 2 cut(s) 182, 481
AxyI CCTNAGG 1 cut(s) 1142
BaeGI GKGCMC 2 cut(s) 498, 1210
BbsI GAAGAC 1 cut(s) 1252
Bbv12I GWGCWC 2 cut(s) 90, 1081
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 3 cut(s) 2, 701, 1054
BccI CCATC 1 cut(s) 485
BceAI ACGGC 4 cut(s) 13, 292, 344, 1133
BciT130I CCWGG 2 cut(s) 128, 684
BclI TGATCA 3 cut(s) 258, 903, 978
BcoDI GTCTC 3 cut(s) 79, 591, 962
BfaI CTAG 1 cut(s) 609
BfoI RGCGCY 1 cut(s) 1068
BglI GCCNNNNNGGC 1 cut(s) 328
BisI GCNGC 6 cut(s) 16, 133, 153, 371, 715, 1068
BlsI GCNGC 6 cut(s) 17, 134, 154, 372, 716, 1069
Bme1390I CCNGG 2 cut(s) 128, 684
Bme18I GGWCC 2 cut(s) 182, 481
BmeRI GACNNNNNGTC 1 cut(s) 71
BmgT120I GGNCC 3 cut(s) 130, 182, 481
BmiI GGNNCC 4 cut(s) 287, 293, 483, 1045
BmrFI CCNGG 2 cut(s) 128, 684
BmrI ACTGGG 1 cut(s) 70
BmsI GCATC 2 cut(s) 246, 637
BmuI ACTGGG 1 cut(s) 70
BpiI GAAGAC 1 cut(s) 1252
BplI GAGNNNNNCTC 2 cut(s) 1280, 1312
BpmI CTGGAG 2 cut(s) 33, 649
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 962
BsaJI CCNNGG 4 cut(s) 126, 127, 326, 355
BsaWI WCCGGW 1 cut(s) 288
Bsc4I CCNNNNNNNGG 7 cut(s) 116, 179, 356, 491, 614, 689, 1285
Bse1I ACTGG 2 cut(s) 65, 632
Bse21I CCTNAGG 1 cut(s) 1142
Bse3DI GCAATG 1 cut(s) 1230
BseBI CCWGG 2 cut(s) 128, 684
BseDI CCNNGG 4 cut(s) 126, 127, 326, 355
BseLI CCNNNNNNNGG 7 cut(s) 116, 179, 356, 491, 614, 689, 1285
BseMI GCAATG 1 cut(s) 1230
BseMII CTCAG 5 cut(s) 69, 90, 105, 332, 957
BseNI ACTGG 2 cut(s) 65, 632
BseRI GAGGAG 5 cut(s) 152, 157, 162, 306, 646
BseSI GKGCMC 2 cut(s) 498, 1210
BseX3I CGGCCG 1 cut(s) 305
BseXI GCAGC 3 cut(s) 2, 701, 1054
Bsh1236I CGCG 2 cut(s) 135, 373
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 3 cut(s) 132, 152, 307
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 2 cut(s) 90, 1081
BsiSI CCGG 1 cut(s) 289
BslFI GGGAC 3 cut(s) 78, 894, 1123
BslI CCNNNNNNNGG 7 cut(s) 116, 179, 356, 491, 614, 689, 1285
BsmAI GTCTC 3 cut(s) 79, 591, 962
BsmFI GGGAC 3 cut(s) 78, 894, 1123
BsmI GAATGC 2 cut(s) 1157, 1292
BsnI GGCC 3 cut(s) 132, 152, 307
Bso31I GGTCTC 1 cut(s) 962
Bsp119I TTCGAA 1 cut(s) 21
Bsp1286I GDGCHC 4 cut(s) 90, 498, 1081, 1210
Bsp143I GATC 4 cut(s) 258, 874, 903, 978
BspACI CCGC 7 cut(s) 133, 153, 207, 371, 612, 972, 993
BspANI GGCC 3 cut(s) 132, 152, 307
BspCNI CTCAG 5 cut(s) 68, 89, 104, 331, 958
BspFNI CGCG 2 cut(s) 135, 373
BspLI GGNNCC 4 cut(s) 287, 293, 483, 1045
BspT104I TTCGAA 1 cut(s) 21
BspTNI GGTCTC 1 cut(s) 962
BsrDI GCAATG 1 cut(s) 1230
BsrI ACTGG 2 cut(s) 65, 632
BssECI CCNNGG 4 cut(s) 126, 127, 326, 355
BssMI GATC 4 cut(s) 258, 874, 903, 978
BssNAI GTATAC 1 cut(s) 790
BssNI GRCGYC 1 cut(s) 5
Bst1107I GTATAC 1 cut(s) 790
Bst2UI CCWGG 2 cut(s) 128, 684
Bst4CI ACNGT 4 cut(s) 145, 441, 670, 808
Bst6I CTCTTC 2 cut(s) 180, 208
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1298
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 3 cut(s) 368, 899, 1081
BstDEI CTNAG 7 cut(s) 55, 76, 91, 111, 318, 966, 1142
BstDSI CCRYGG 1 cut(s) 326
BstENI CCTNNNNNAGG 3 cut(s) 114, 177, 612
BstFNI CGCG 2 cut(s) 135, 373
BstH2I RGCGCY 1 cut(s) 1068
BstHHI GCGC 2 cut(s) 375, 1067
BstKTI GATC 4 cut(s) 261, 877, 906, 981
BstMAI GTCTC 3 cut(s) 79, 591, 962
BstMBI GATC 4 cut(s) 258, 874, 903, 978
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 5 cut(s) 328, 372, 1076, 1273, 1298
BstNI CCWGG 2 cut(s) 128, 684
BstNSI RCATGY 2 cut(s) 538, 993
BstSCI CCNGG 2 cut(s) 126, 682
BstSLI GKGCMC 2 cut(s) 498, 1210
BstUI CGCG 2 cut(s) 135, 373
BstV1I GCAGC 3 cut(s) 2, 701, 1054
BstV2I GAAGAC 1 cut(s) 1252
BstXI CCANNNNNNTGG 1 cut(s) 927
BstZ17I GTATAC 1 cut(s) 790
BstZI CGGCCG 1 cut(s) 305
Bsu36I CCTNAGG 1 cut(s) 1142
BsuRI GGCC 3 cut(s) 132, 152, 307
BtgI CCRYGG 1 cut(s) 326
BtgZI GCGATG 2 cut(s) 347, 465
Cac8I GCNNGC 3 cut(s) 368, 899, 1081
CfoI GCGC 2 cut(s) 375, 1067
Cfr13I GGNCC 3 cut(s) 130, 182, 481
CseI GACGC 1 cut(s) 290
CviAII CATG 6 cut(s) 535, 872, 990, 1028, 1169, 1211
DdeI CTNAG 7 cut(s) 55, 76, 91, 111, 318, 966, 1142
DpnI GATC 4 cut(s) 260, 876, 905, 980
DpnII GATC 4 cut(s) 258, 874, 903, 978
DriI GACNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 2 cut(s) 150, 305
EagI CGGCCG 1 cut(s) 305
Eam1104I CTCTTC 2 cut(s) 180, 208
Eam1105I GACNNNNNGTC 1 cut(s) 71
EarI CTCTTC 2 cut(s) 180, 208
EclXI CGGCCG 1 cut(s) 305
Eco31I GGTCTC 1 cut(s) 962
Eco32I GATATC 1 cut(s) 335
Eco47I GGWCC 2 cut(s) 182, 481
Eco52I CGGCCG 1 cut(s) 305
Eco81I CCTNAGG 1 cut(s) 1142
EcoNI CCTNNNNNAGG 3 cut(s) 114, 177, 612
EcoO109I RGGNCCY 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 126, 682
EcoRV GATATC 1 cut(s) 335
FaeI CATG 6 cut(s) 538, 875, 993, 1031, 1172, 1214
FalI AAGNNNNNCTT 2 cut(s) 1023, 1055
FaqI GGGAC 3 cut(s) 78, 894, 1123
FatI CATG 6 cut(s) 534, 871, 989, 1027, 1168, 1210
FauI CCCGC 1 cut(s) 986
FbaI TGATCA 3 cut(s) 258, 903, 978
FblI GTMKAC 3 cut(s) 96, 789, 1091
Fnu4HI GCNGC 6 cut(s) 16, 133, 153, 371, 715, 1068
Fsp4HI GCNGC 6 cut(s) 16, 133, 153, 371, 715, 1068
FspBI CTAG 1 cut(s) 609
GlaI GCGC 2 cut(s) 374, 1066
GluI GCNGC 6 cut(s) 16, 133, 153, 371, 715, 1068
GsuI CTGGAG 2 cut(s) 33, 649
HaeII RGCGCY 1 cut(s) 1068
HaeIII GGCC 3 cut(s) 132, 152, 307
HapII CCGG 1 cut(s) 289
HgaI GACGC 1 cut(s) 290
HhaI GCGC 2 cut(s) 375, 1067
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 538, 875, 993, 1031, 1172, 1214
Hin6I GCGC 2 cut(s) 373, 1065
HinP1I GCGC 2 cut(s) 373, 1065
HincII GTYRAC 1 cut(s) 341
HindII GTYRAC 1 cut(s) 341
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 3 cut(s) 53, 345, 1097
HpaII CCGG 1 cut(s) 289
HphI GGTGA 4 cut(s) 133, 561, 597, 704
Hpy166II GTNNAC 5 cut(s) 97, 312, 341, 790, 1092
Hpy188I TCNGA 8 cut(s) 58, 79, 263, 384, 721, 832, 858, 967
Hpy188III TCNNGA 2 cut(s) 50, 1190
Hpy8I GTNNAC 5 cut(s) 97, 312, 341, 790, 1092
Hpy99I CGWCG 3 cut(s) 10, 34, 306
HpyAV CCTTC 4 cut(s) 51, 166, 511, 781
HpyCH4III ACNGT 4 cut(s) 145, 441, 670, 808
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 8 cut(s) 237, 269, 532, 871, 1027, 1070, 1110, 1223
HpyF10VI GCNNNNNNNGC 5 cut(s) 328, 372, 1076, 1273, 1298
HpyF3I CTNAG 7 cut(s) 55, 76, 91, 111, 318, 966, 1142
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 538, 875, 993, 1031, 1172, 1214
HspAI GCGC 2 cut(s) 373, 1065
Ksp22I TGATCA 3 cut(s) 258, 903, 978
Kzo9I GATC 4 cut(s) 258, 874, 903, 978
LmnI GCTCC 5 cut(s) 111, 633, 758, 940, 1076
Lsp1109I GCAGC 3 cut(s) 2, 701, 1054
LweI GCATC 2 cut(s) 246, 637
MaeI CTAG 1 cut(s) 609
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 139
MalI GATC 4 cut(s) 260, 876, 905, 980
MboI GATC 4 cut(s) 258, 874, 903, 978
MboII GAAGA 7 cut(s) 36, 167, 195, 770, 789, 1065, 1257
MhlI GDGCHC 4 cut(s) 90, 498, 1081, 1210
MluCI AATT 9 cut(s) 251, 264, 402, 476, 527, 660, 953, 1194, 1261
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 5 cut(s) 503, 716, 727, 790, 989
MroXI GAANNNNTTC 2 cut(s) 837, 1035
MseI TTAA 3 cut(s) 378, 638, 1197
MslI CAYNNNNRTG 2 cut(s) 876, 1173
MspI CCGG 1 cut(s) 289
MspR9I CCNGG 2 cut(s) 128, 684
Mva1269I GAATGC 2 cut(s) 1157, 1292
MvaI CCWGG 2 cut(s) 128, 684
MvnI CGCG 2 cut(s) 135, 373
MwoI GCNNNNNNNGC 5 cut(s) 328, 372, 1076, 1273, 1298
NdeII GATC 4 cut(s) 258, 874, 903, 978
NlaIII CATG 6 cut(s) 538, 875, 993, 1031, 1172, 1214
NlaIV GGNNCC 4 cut(s) 287, 293, 483, 1045
NmeAIII GCCGAG 1 cut(s) 380
NmuCI GTSAC 1 cut(s) 139
NspI RCATGY 2 cut(s) 538, 993
NspV TTCGAA 1 cut(s) 21
PasI CCCWGGG 1 cut(s) 127
PciI ACATGT 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 334
PctI GAATGC 2 cut(s) 1157, 1292
PdmI GAANNNNTTC 2 cut(s) 837, 1035
PfeI GAWTC 2 cut(s) 345, 1097
PkrI GCNGC 6 cut(s) 17, 134, 154, 372, 716, 1069
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PpuMI RGGWCCY 1 cut(s) 182
PscI ACATGT 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 182
Psp6I CCWGG 2 cut(s) 126, 682
PspGI CCWGG 2 cut(s) 126, 682
PspN4I GGNNCC 4 cut(s) 287, 293, 483, 1045
PspPI GGNCC 3 cut(s) 130, 182, 481
PspPPI RGGWCCY 1 cut(s) 182
RseI CAYNNNNRTG 2 cut(s) 876, 1173
SaqAI TTAA 3 cut(s) 378, 638, 1197
SatI GCNGC 6 cut(s) 16, 133, 153, 371, 715, 1068
Sau3AI GATC 4 cut(s) 258, 874, 903, 978
Sau96I GGNCC 3 cut(s) 130, 182, 481
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 128, 684
SduI GDGCHC 4 cut(s) 90, 498, 1081, 1210
SfaNI GCATC 2 cut(s) 246, 637
SfuI TTCGAA 1 cut(s) 21
SinI GGWCC 2 cut(s) 182, 481
SmiMI CAYNNNNRTG 2 cut(s) 876, 1173
Sse9I AATT 9 cut(s) 251, 264, 402, 476, 527, 660, 953, 1194, 1261
SsiI CCGC 7 cut(s) 133, 153, 207, 371, 612, 972, 993
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 2 cut(s) 126, 682
TaaI ACNGT 4 cut(s) 145, 441, 670, 808
TaiI ACGT 1 cut(s) 8
TaqI TCGA 2 cut(s) 21, 1189
TasI AATT 9 cut(s) 251, 264, 402, 476, 527, 660, 953, 1194, 1261
TauI GCSGC 3 cut(s) 135, 155, 373
TfiI GAWTC 2 cut(s) 345, 1097
Tru1I TTAA 3 cut(s) 378, 638, 1197
Tru9I TTAA 3 cut(s) 378, 638, 1197
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 3 cut(s) 15, 714, 1067
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 5 cut(s) 567, 611, 1044, 1164, 1227
TspGWI ACGGA 2 cut(s) 513, 1077
VpaK11BI GGWCC 2 cut(s) 182, 481
XagI CCTNNNNNAGG 3 cut(s) 114, 177, 612
XapI RAATTY 2 cut(s) 402, 953
XceI RCATGY 2 cut(s) 538, 993
XcmI CCANNNNNNNNNTGG 1 cut(s) 57
XmiI GTMKAC 3 cut(s) 96, 789, 1091
XmnI GAANNNNTTC 2 cut(s) 837, 1035
XspI CTAG 1 cut(s) 609
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.