RchiOBHm_Chr1g0355831
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003990
Physical Location & Seq
Forward (+)
48405097 .. 48409173
4077 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGTGTGGTAACATTCAGATCCTTACTGCAGCTGGTCTCGATAGATATGGAGCGAATGTATGGCTTCTGTCTCCTCCTCGCCAACCCTATACTAGACAAGGTCTCCAAAAGCAATCTGGTGATGCTCAGGCATTTTCATTTCTCCAGATTCTTGAGTTGATACCACACACCTCGCAACCTCCAATAATGTTATTTGTGGAAAATGTTGTTGGATTTGAGACATCTGATACACATGGAAAAATGATAGAGATATTGAGAAGAAGTGATTTTGTTACACAGGAGTTCATATTGAGTCCCTTGCAGTTTGGTGTACCAATAGAAAGCTTTCTTGAATTCAAGAATTGCCGTGATCAACCGAACTTTGTTGACAACACCACTGTTTATACAGATACCATTGGAGTATTGGAGAAAGATGAAGAGAATGGGTGTTGTACTAGCACCTTGGATCAGTACTCTGTTCCCTCAAGCTTGATAGAGAGATGGGGAAGTGCCATGGATATTGTCTACCCGGATTCAAAGCGATGCTGTTGTTTTACAAAAAGTTATTATCGATATGTGAAGGGTACTGGCTCCCTTTTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

194

Amino Acids

21.86

Weight (kDa)

4.92

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 504
AclWI GGATC 2 cut(s) 13, 453
AcsI RAATTY 1 cut(s) 332
AfaI GTAC 4 cut(s) 312, 433, 452, 565
AgsI TTSAA 3 cut(s) 332, 337, 516
AjuI GAANNNNNNNTTGG 2 cut(s) 192, 224
AluBI AGCT 3 cut(s) 32, 324, 468
AluI AGCT 3 cut(s) 32, 324, 468
Alw26I GTCTC 4 cut(s) 41, 75, 107, 212
AlwI GGATC 2 cut(s) 13, 453
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 332
Asp700I GAANNNNTTC 1 cut(s) 324
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 1 cut(s) 131
BbvI GCAGC 1 cut(s) 41
BccI CCATC 1 cut(s) 474
BceAI ACGGC 1 cut(s) 330
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 4 cut(s) 41, 75, 107, 212
BfaI CTAG 2 cut(s) 93, 435
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 1 cut(s) 509
BmiI GGNNCC 1 cut(s) 571
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 2 cut(s) 112, 512
BpmI CTGGAG 1 cut(s) 128
Bpu10I CCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 173, 448
BpuMI CCSGG 1 cut(s) 509
Bsa29I ATCGAT 1 cut(s) 550
BsaI GGTCTC 2 cut(s) 41, 107
BsaJI CCNNGG 2 cut(s) 441, 492
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 87, 117
Bse1I ACTGG 1 cut(s) 571
BseCI ATCGAT 1 cut(s) 550
BseDI CCNNGG 2 cut(s) 441, 492
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 571
BseRI GAGGAG 2 cut(s) 63, 66
BseXI GCAGC 1 cut(s) 41
BshVI ATCGAT 1 cut(s) 550
BsiSI CCGG 1 cut(s) 509
BslFI GGGAC 1 cut(s) 279
BsmAI GTCTC 4 cut(s) 41, 75, 107, 212
BsmFI GGGAC 1 cut(s) 279
Bso31I GGTCTC 2 cut(s) 41, 107
Bsp143I GATC 3 cut(s) 18, 349, 445
Bsp19I CCATGG 1 cut(s) 492
BspCNI CTCAG 1 cut(s) 139
BspDI ATCGAT 1 cut(s) 550
BspLI GGNNCC 1 cut(s) 571
BspMAI CTGCAG 1 cut(s) 31
BspPI GGATC 2 cut(s) 13, 453
BspTNI GGTCTC 2 cut(s) 41, 107
BsrI ACTGG 1 cut(s) 571
BssECI CCNNGG 2 cut(s) 441, 492
BssMI GATC 3 cut(s) 18, 349, 445
BssT1I CCWWGG 2 cut(s) 441, 492
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 1 cut(s) 411
BstDEI CTNAG 1 cut(s) 126
BstDSI CCRYGG 1 cut(s) 492
BstKTI GATC 3 cut(s) 21, 352, 448
BstMAI GTCTC 4 cut(s) 41, 75, 107, 212
BstMBI GATC 3 cut(s) 18, 349, 445
BstSCI CCNGG 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
Bsu15I ATCGAT 1 cut(s) 550
BsuTUI ATCGAT 1 cut(s) 550
BtgI CCRYGG 1 cut(s) 492
BtgZI GCGATG 1 cut(s) 535
BtsIMutI CAGTG 1 cut(s) 375
ClaI ATCGAT 1 cut(s) 550
Csp6I GTAC 4 cut(s) 311, 432, 451, 564
CviAII CATG 3 cut(s) 233, 493, 582
CviJI RGCY 5 cut(s) 32, 64, 324, 468, 570
CviKI_1 RGCY 5 cut(s) 32, 64, 324, 468, 570
CviQI GTAC 4 cut(s) 311, 432, 451, 564
DdeI CTNAG 1 cut(s) 126
DpnI GATC 3 cut(s) 20, 351, 447
DpnII GATC 3 cut(s) 18, 349, 445
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco130I CCWWGG 2 cut(s) 441, 492
Eco31I GGTCTC 2 cut(s) 41, 107
EcoRI GAATTC 1 cut(s) 332
EcoT14I CCWWGG 2 cut(s) 441, 492
ErhI CCWWGG 2 cut(s) 441, 492
FaeI CATG 3 cut(s) 236, 496, 585
FaiI YATR 9 cut(s) 48, 61, 90, 234, 287, 384, 494, 555, 583
FaqI GGGAC 1 cut(s) 279
FatI CATG 3 cut(s) 232, 492, 581
FbaI TGATCA 1 cut(s) 349
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 2 cut(s) 93, 435
GluI GCNGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 128
HapII CCGG 1 cut(s) 509
Hin1II CATG 3 cut(s) 236, 496, 585
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HindIII AAGCTT 2 cut(s) 322, 466
HinfI GANTC 3 cut(s) 148, 292, 512
HpaII CCGG 1 cut(s) 509
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 3 cut(s) 311, 367, 505
Hpy188I TCNGA 2 cut(s) 18, 226
Hpy188III TCNNGA 5 cut(s) 38, 145, 152, 329, 337
Hpy8I GTNNAC 3 cut(s) 311, 367, 505
HpyAV CCTTC 1 cut(s) 553
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4V TGCA 2 cut(s) 29, 301
HpyF3I CTNAG 1 cut(s) 126
Hsp92II CATG 3 cut(s) 236, 496, 585
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 3 cut(s) 18, 349, 445
LmnI GCTCC 2 cut(s) 50, 575
LpnPI CCDG 7 cut(s) 18, 102, 113, 158, 263, 522, 552
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 2 cut(s) 112, 512
MaeI CTAG 2 cut(s) 93, 435
MaeIII GTNAC 2 cut(s) 8, 271
MalI GATC 3 cut(s) 20, 351, 447
MboI GATC 3 cut(s) 18, 349, 445
MboII GAAGA 2 cut(s) 270, 428
MflI RGATCY 1 cut(s) 18
MluCI AATT 2 cut(s) 332, 340
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 5 cut(s) 84, 87, 181, 189, 472
MroXI GAANNNNTTC 1 cut(s) 324
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 1 cut(s) 509
NciI CCSGG 1 cut(s) 509
NcoI CCATGG 1 cut(s) 492
NdeII GATC 3 cut(s) 18, 349, 445
NlaIII CATG 3 cut(s) 236, 496, 585
NlaIV GGNNCC 1 cut(s) 571
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 2 cut(s) 148, 512
PflFI GACNNNGTC 1 cut(s) 99
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PspN4I GGNNCC 1 cut(s) 571
PstI CTGCAG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 18
PsyI GACNNNGTC 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 4 cut(s) 312, 433, 452, 565
RsaNI GTAC 4 cut(s) 311, 432, 451, 564
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 3 cut(s) 18, 349, 445
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 509
SetI ASST 7 cut(s) 34, 103, 173, 181, 326, 443, 470
SfaNI GCATC 2 cut(s) 112, 512
SfcI CTRYAG 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 152, 463
SmoI CTYRAG 2 cut(s) 152, 463
Sse9I AATT 2 cut(s) 332, 340
SspMI CTAG 2 cut(s) 93, 435
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 2 cut(s) 441, 492
TaaI ACNGT 1 cut(s) 379
TaqI TCGA 2 cut(s) 39, 550
TasI AATT 2 cut(s) 332, 340
TatI WGTACW 2 cut(s) 431, 450
TfiI GAWTC 2 cut(s) 148, 512
TscAI CASTG 1 cut(s) 382
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 126, 274, 429
TspRI CASTG 1 cut(s) 382
Tth111I GACNNNGTC 1 cut(s) 99
XapI RAATTY 1 cut(s) 332
XcmI CCANNNNNNNNNTGG 2 cut(s) 113, 400
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 2 cut(s) 93, 435
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.