RchiOBHm_Chr0c38g0503051

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_chinensis
Unknown
OG0004217
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 867 bp
ATGAAGGGAGTGAGAAAGTTAAGTCGAAAGATCTTTAAAAAGATTTGCAAACGTGATGGAGATCTTTGGAGATATTACGAGGACGCTTGCAAGAATGAAGAAGATTTCCTAACTGAACTGGGGAAGTTCCTAGAGTCGTATGAAAAATGTTTGATTCAAATTACGAAGGTGGGAAACAACGACGGAGGAATTGATGATGGTTACATGAGGATTTTGGAGAAATTGAAGAATGTCAAGGGGGCCTCAGGTAGAGTCCATGATCAGCATTTTGCGGAACAAATGTTGCTCGAAAATGTTAAATGTTTGATGAAGCGGCGAGAAGATTTGCATGAAGAATTGATTAGCCAAAAAGGAAAGCCTCGCAAGAAGTATGATTGTACTCGTGCTTGGAGACGCTGTTTGAGGATGGTCACTAATATATTGTTCATTGGTGCTGCAGCTGCTGAGTTTGCCTGCACAGTTGTGGCAGCAGTTATGGCTGCTCCACTTGTTGCACTGGCCGTTGCTGCTACTATTATCCCAACACTAGCCGGACAGCATTGGACTCTGTCTTGGATTGAGGATTGTGAAATTCCTTTCGAGTATCCGGATGATGTAATTCGCTTAATGCAATCCGGAACTGTGCTTTCCATTAAGGAATTGGGAGAAATTCAGACGCGTCTCGATCGTGTTGTAAATGACATAAAATCACTCTTATCCCATCTCAACTCTGTAATTGAGGATGAAGTAAATCTCAATATGAAAAGTATTGAGCGTCTTCTCAAGAACATTAATGAATTGATGGAAAGAGCAAGGAATTCCAAGTCCCAGATATTAGAGGCAAGAGATGAGGTTTCTACGAGCATCAAGATCACGAAGGGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

288

Amino Acids

32.9

Weight (kDa)

8.52

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 59 - 278 4.6e-18 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 658
AccIII TCCGGA 2 cut(s) 586, 614
AciI CCGC 2 cut(s) 272, 313
AcoI YGGCCR 1 cut(s) 498
AcsI RAATTY 3 cut(s) 570, 648, 796
AfaI GTAC 1 cut(s) 379
AflIII ACRYGT 1 cut(s) 656
AgsI TTSAA 2 cut(s) 158, 226
AleI CACNNNNGTG 1 cut(s) 461
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw26I GTCTC 2 cut(s) 385, 665
AlwNI CAGNNNCTG 1 cut(s) 443
Aor13HI TCCGGA 2 cut(s) 586, 614
AoxI GGCC 2 cut(s) 240, 498
ApeKI GCWGC 6 cut(s) 434, 437, 440, 467, 479, 506
ApoI RAATTY 3 cut(s) 570, 648, 796
AseI ATTAAT 1 cut(s) 771
AspS9I GGNCC 1 cut(s) 240
AxyI CCTNAGG 1 cut(s) 244
BauI CACGAG 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 749
BbvI GCAGC 6 cut(s) 421, 427, 449, 466, 479, 493
BccI CCATC 5 cut(s) 50, 191, 400, 708, 775
BceAI ACGGC 1 cut(s) 485
BciVI GTATCC 1 cut(s) 594
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 2 cut(s) 385, 665
BfaI CTAG 2 cut(s) 131, 527
BfmI CTRYAG 1 cut(s) 435
BfuI GTATCC 1 cut(s) 594
BglII AGATCT 2 cut(s) 30, 61
BisI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
BlsI GCNGC 7 cut(s) 315, 436, 439, 442, 469, 481, 508
BmgT120I GGNCC 1 cut(s) 240
BmiI GGNNCC 1 cut(s) 241
BmrI ACTGGG 1 cut(s) 128
BmsI GCATC 1 cut(s) 852
BmuI ACTGGG 1 cut(s) 128
BpiI GAAGAC 1 cut(s) 749
BpuEI CTTGAG 1 cut(s) 746
BsaBI GATNNNNATC 1 cut(s) 60
BsaWI WCCGGW 2 cut(s) 586, 614
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bse1I ACTGG 2 cut(s) 123, 501
Bse21I CCTNAGG 1 cut(s) 244
Bse8I GATNNNNATC 1 cut(s) 60
BseAI TCCGGA 2 cut(s) 586, 614
BseGI GGATG 3 cut(s) 411, 595, 727
BseJI GATNNNNATC 1 cut(s) 60
BseMII CTCAG 2 cut(s) 258, 435
BseNI ACTGG 2 cut(s) 123, 501
BseXI GCAGC 6 cut(s) 421, 427, 449, 466, 479, 493
BsgI GTGCAG 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 658
Bsh1285I CGRYCG 1 cut(s) 667
BshFI GGCC 2 cut(s) 242, 500
BsiEI CGRYCG 1 cut(s) 667
BsiSI CCGG 3 cut(s) 531, 587, 615
BslFI GGGAC 1 cut(s) 790
BsmAI GTCTC 2 cut(s) 385, 665
BsmBI CGTCTC 2 cut(s) 385, 665
BsmFI GGGAC 1 cut(s) 790
BsnI GGCC 2 cut(s) 242, 500
Bsp13I TCCGGA 2 cut(s) 586, 614
Bsp143I GATC 5 cut(s) 30, 61, 259, 664, 849
BspACI CCGC 2 cut(s) 272, 313
BspANI GGCC 2 cut(s) 242, 500
BspCNI CTCAG 2 cut(s) 257, 436
BspEI TCCGGA 2 cut(s) 586, 614
BspFNI CGCG 1 cut(s) 658
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 439
BsrI ACTGG 2 cut(s) 123, 501
BssMI GATC 5 cut(s) 30, 61, 259, 664, 849
BssSI CACGAG 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 381
Bst4CI ACNGT 2 cut(s) 460, 622
BstC8I GCNNGC 2 cut(s) 88, 454
BstDEI CTNAG 2 cut(s) 244, 444
BstF5I GGATG 3 cut(s) 411, 595, 727
BstFNI CGCG 1 cut(s) 658
BstKTI GATC 5 cut(s) 33, 64, 262, 667, 852
BstMAI GTCTC 2 cut(s) 385, 665
BstMBI GATC 5 cut(s) 30, 61, 259, 664, 849
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 4 cut(s) 440, 449, 476, 506
BstSFI CTRYAG 1 cut(s) 435
BstUI CGCG 1 cut(s) 658
BstV1I GCAGC 6 cut(s) 421, 427, 449, 466, 479, 493
BstV2I GAAGAC 1 cut(s) 749
BstX2I RGATCY 2 cut(s) 30, 61
BstYI RGATCY 2 cut(s) 30, 61
Bsu36I CCTNAGG 1 cut(s) 244
BsuI GTATCC 1 cut(s) 594
BsuRI GGCC 2 cut(s) 242, 500
BtsCI GGATG 3 cut(s) 411, 595, 727
BtsIMutI CAGTG 1 cut(s) 494
Cac8I GCNNGC 2 cut(s) 88, 454
CaiI CAGNNNCTG 1 cut(s) 443
Cfr13I GGNCC 1 cut(s) 240
CseI GACGC 5 cut(s) 92, 402, 647, 664, 743
Csp6I GTAC 1 cut(s) 378
CviAII CATG 3 cut(s) 205, 257, 329
CviJI RGCY 7 cut(s) 242, 345, 358, 440, 479, 500, 530
CviKI_1 RGCY 7 cut(s) 242, 345, 358, 440, 479, 500, 530
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 2 cut(s) 244, 444
DpnI GATC 5 cut(s) 32, 63, 261, 666, 851
DpnII GATC 5 cut(s) 30, 61, 259, 664, 849
DraI TTTAAA 1 cut(s) 37
EaeI YGGCCR 1 cut(s) 498
Eco81I CCTNAGG 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 240
EcoRI GAATTC 1 cut(s) 796
Esp3I CGTCTC 2 cut(s) 385, 665
FaeI CATG 3 cut(s) 208, 260, 332
FaqI GGGAC 1 cut(s) 790
FatI CATG 3 cut(s) 204, 256, 328
FbaI TGATCA 1 cut(s) 259
Fnu4HI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
FokI GGATG 3 cut(s) 418, 602, 734
Fsp4HI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
FspBI CTAG 2 cut(s) 131, 527
GluI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
HaeIII GGCC 2 cut(s) 242, 500
HapII CCGG 3 cut(s) 531, 587, 615
HgaI GACGC 5 cut(s) 92, 402, 647, 664, 743
Hin1II CATG 3 cut(s) 208, 260, 332
HinfI GANTC 4 cut(s) 134, 154, 252, 544
HpaII CCGG 3 cut(s) 531, 587, 615
Hpy188I TCNGA 1 cut(s) 654
Hpy188III TCNNGA 6 cut(s) 587, 615, 662, 763, 847, 853
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 160, 850
HpyCH4III ACNGT 2 cut(s) 460, 622
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 7 cut(s) 48, 90, 328, 437, 456, 494, 610
HpyF10VI GCNNNNNNNGC 4 cut(s) 440, 449, 476, 506
HpyF3I CTNAG 2 cut(s) 244, 444
HpySE526I ACGT 1 cut(s) 52
Hsp92II CATG 3 cut(s) 208, 260, 332
Kpn2I TCCGGA 2 cut(s) 586, 614
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 5 cut(s) 30, 61, 259, 664, 849
LmnI GCTCC 1 cut(s) 487
LpnPI CCDG 8 cut(s) 104, 231, 466, 482, 544, 600, 628, 821
Lsp1109I GCAGC 6 cut(s) 421, 427, 449, 466, 479, 493
LweI GCATC 1 cut(s) 852
MaeI CTAG 2 cut(s) 131, 527
MaeII ACGT 1 cut(s) 52
MaeIII GTNAC 2 cut(s) 200, 409
MalI GATC 5 cut(s) 32, 63, 261, 666, 851
MboI GATC 5 cut(s) 30, 61, 259, 664, 849
MboII GAAGA 6 cut(s) 110, 113, 238, 332, 344, 749
MflI RGATCY 2 cut(s) 30, 61
MluI ACGCGT 1 cut(s) 656
MlyI GAGTC 3 cut(s) 143, 261, 538
MroI TCCGGA 2 cut(s) 586, 614
MseI TTAA 6 cut(s) 20, 36, 297, 605, 633, 771
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 3 cut(s) 531, 587, 615
MvnI CGCG 1 cut(s) 658
MwoI GCNNNNNNNGC 4 cut(s) 440, 449, 476, 506
NdeII GATC 5 cut(s) 30, 61, 259, 664, 849
NlaIII CATG 3 cut(s) 208, 260, 332
NlaIV GGNNCC 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 409
OliI CACNNNNGTG 1 cut(s) 461
PfeI GAWTC 1 cut(s) 154
PflFI GACNNNGTC 1 cut(s) 547
PkrI GCNGC 7 cut(s) 315, 436, 439, 442, 469, 481, 508
Ple19I CGATCG 1 cut(s) 667
PleI GAGTC 3 cut(s) 142, 260, 538
PpsI GAGTC 3 cut(s) 142, 260, 538
PshBI ATTAAT 1 cut(s) 771
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 1 cut(s) 240
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 2 cut(s) 30, 61
PsyI GACNNNGTC 1 cut(s) 547
PvuI CGATCG 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 6 cut(s) 20, 36, 297, 605, 633, 771
SatI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
Sau3AI GATC 5 cut(s) 30, 61, 259, 664, 849
Sau96I GGNCC 1 cut(s) 240
SchI GAGTC 3 cut(s) 143, 261, 538
SetI ASST 5 cut(s) 55, 171, 250, 442, 834
SfaNI GCATC 1 cut(s) 852
SfcI CTRYAG 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
SsiI CCGC 2 cut(s) 272, 313
SspMI CTAG 2 cut(s) 131, 527
TaaI ACNGT 2 cut(s) 460, 622
TaiI ACGT 1 cut(s) 55
TaqI TCGA 4 cut(s) 25, 288, 579, 663
TatI WGTACW 1 cut(s) 377
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 6 cut(s) 20, 36, 297, 605, 633, 771
Tru9I TTAA 6 cut(s) 20, 36, 297, 605, 633, 771
TscAI CASTG 1 cut(s) 501
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 6 cut(s) 434, 437, 440, 467, 479, 506
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 9 cut(s) 17, 111, 156, 323, 345, 415, 738, 755, 789
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 1 cut(s) 501
Tth111I GACNNNGTC 1 cut(s) 547
VspI ATTAAT 1 cut(s) 771
XapI RAATTY 3 cut(s) 570, 648, 796
XcmI CCANNNNNNNNNTGG 1 cut(s) 637
XspI CTAG 2 cut(s) 131, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.