Rroxscaffold_177G00434280

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000177
OG0004217
Physical Location & Seq
Reverse (-)
247609 .. 249362
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_177G00434280.1

Sequence Viewer

Length: 660 bp
ATGCAGAGCTGTTGGTTACCGGAGCAAGAGTCGTATGAAAAATGTTTGATTCAAATTACGAAGGTGGGAAACAACGACGGAGGAATTGATGATGGTTACATGAGGATTTTGGAGAAATTGAAGAATGTCAAGGGGCCCTCAGGTAGAGTCCATGATCAGCATTTTGCAGAACAAATGTTGCTCGAAAAGGTTAAATGTTTGATGACGCGGCGAGAAGATTTGCATGAAGAATTGATTAGCCAAAAAGGAAAGCCTCGCAAGAAGTATGATTGTACTCGTGCTTGGAGACGCTGTTTAAGGATGGTCACTCATATATTGTTCATTGGTCCTGCAGCTGTTGAGTTTGCCTGCACAGTTGTGGCAGCAGTTATGGCTGCTCCACTTGTTGCACTGGCCATTGCTGCTGCTATTATCCCAACACTAGCCGGACAGCATTGGACTCTGTCTTGGATTGAAGATTGTGAAATTCCTTTCGAGTATCCGGACGATGTAATTTGCTTGATGCAATCCGGAACTGTGATTTCCATTAAGGAATTGGGAGAAATTCAGACGTGTCTCTATCGTGTTGTAAATGACATAAAATCACTCTTATCCCATCTCAACTCTGTAATTGAGGGTGAAGTAAATCTCAATATGAAAAGTATTGTTGGAAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

219

Amino Acids

24.61

Weight (kDa)

6.31

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 25 - 209 1.5e-12 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 208
AccIII TCCGGA 2 cut(s) 481, 509
AciI CCGC 1 cut(s) 208
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 2 cut(s) 465, 543
AfaI GTAC 1 cut(s) 274
AflIII ACRYGT 1 cut(s) 551
AgsI TTSAA 3 cut(s) 53, 121, 455
AjiI CACGTC 1 cut(s) 552
AleI CACNNNNGTG 1 cut(s) 356
AluBI AGCT 3 cut(s) 9, 335, 657
AluI AGCT 3 cut(s) 9, 335, 657
Alw26I GTCTC 2 cut(s) 280, 560
Aor13HI TCCGGA 2 cut(s) 481, 509
AoxI GGCC 2 cut(s) 134, 393
ApaI GGGCCC 1 cut(s) 138
ApeKI GCWGC 5 cut(s) 332, 362, 374, 401, 404
ApoI RAATTY 2 cut(s) 465, 543
AspS9I GGNCC 3 cut(s) 134, 135, 326
AsuHPI GGTGA 1 cut(s) 629
AvaII GGWCC 1 cut(s) 326
AxyI CCTNAGG 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 138
BalI TGGCCA 1 cut(s) 395
BanII GRGCYC 1 cut(s) 138
BauI CACGAG 1 cut(s) 276
BbvI GCAGC 5 cut(s) 344, 361, 374, 388, 391
BccI CCATC 3 cut(s) 86, 295, 603
BciVI GTATCC 1 cut(s) 489
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 280, 560
BfaI CTAG 1 cut(s) 422
BfmI CTRYAG 1 cut(s) 330
BfuI GTATCC 1 cut(s) 489
BisI GCNGC 6 cut(s) 209, 333, 363, 375, 402, 405
BlsI GCNGC 6 cut(s) 210, 334, 364, 376, 403, 406
Bme18I GGWCC 1 cut(s) 326
BmgBI CACGTC 1 cut(s) 552
BmgT120I GGNCC 3 cut(s) 134, 135, 326
BmiI GGNNCC 2 cut(s) 135, 136
BmsI GCATC 1 cut(s) 492
BsaWI WCCGGW 3 cut(s) 19, 481, 509
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 277, 307
Bse1I ACTGG 1 cut(s) 396
Bse21I CCTNAGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 396
BseAI TCCGGA 2 cut(s) 481, 509
BseGI GGATG 1 cut(s) 306
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 396
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 5 cut(s) 344, 361, 374, 388, 391
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 1 cut(s) 208
BshFI GGCC 2 cut(s) 136, 395
BsiSI CCGG 4 cut(s) 20, 426, 482, 510
BsmAI GTCTC 2 cut(s) 280, 560
BsmBI CGTCTC 1 cut(s) 280
BsnI GGCC 2 cut(s) 136, 395
Bsp120I GGGCCC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 138
Bsp13I TCCGGA 2 cut(s) 481, 509
Bsp143I GATC 1 cut(s) 154
BspACI CCGC 1 cut(s) 208
BspANI GGCC 2 cut(s) 136, 395
BspCNI CTCAG 1 cut(s) 152
BspEI TCCGGA 2 cut(s) 481, 509
BspFNI CGCG 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 135, 136
BspMAI CTGCAG 1 cut(s) 334
BsrDI GCAATG 1 cut(s) 396
BsrI ACTGG 1 cut(s) 396
BssMI GATC 1 cut(s) 154
BssSI CACGAG 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 276
Bst4CI ACNGT 2 cut(s) 355, 517
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 1 cut(s) 139
BstEII GGTNACC 1 cut(s) 15
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 208
BstKTI GATC 1 cut(s) 157
BstMAI GTCTC 2 cut(s) 280, 560
BstMBI GATC 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 371, 401
BstPI GGTNACC 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 330
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 1 cut(s) 208
BstV1I GCAGC 5 cut(s) 344, 361, 374, 388, 391
Bsu36I CCTNAGG 1 cut(s) 139
BsuI GTATCC 1 cut(s) 489
BsuRI GGCC 2 cut(s) 136, 395
BtrI CACGTC 1 cut(s) 552
BtsCI GGATG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 389
Cac8I GCNNGC 1 cut(s) 349
Cfr13I GGNCC 3 cut(s) 134, 135, 326
CseI GACGC 2 cut(s) 214, 297
Csp6I GTAC 1 cut(s) 273
CviAII CATG 3 cut(s) 100, 152, 224
CviJI RGCY 9 cut(s) 9, 136, 240, 253, 335, 374, 395, 425, 657
CviKI_1 RGCY 9 cut(s) 9, 136, 240, 253, 335, 374, 395, 425, 657
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 1 cut(s) 139
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 326
Eco81I CCTNAGG 1 cut(s) 139
Eco91I GGTNACC 1 cut(s) 15
EcoO109I RGGNCCY 2 cut(s) 134, 135
EcoO65I GGTNACC 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 3 cut(s) 103, 155, 227
FatI CATG 3 cut(s) 99, 151, 223
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 6 cut(s) 209, 333, 363, 375, 402, 405
FokI GGATG 1 cut(s) 313
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 6 cut(s) 209, 333, 363, 375, 402, 405
FspBI CTAG 1 cut(s) 422
GluI GCNGC 6 cut(s) 209, 333, 363, 375, 402, 405
HaeIII GGCC 2 cut(s) 136, 395
HapII CCGG 4 cut(s) 20, 426, 482, 510
HgaI GACGC 2 cut(s) 214, 297
Hin1II CATG 3 cut(s) 103, 155, 227
HinfI GANTC 4 cut(s) 29, 49, 147, 439
HpaII CCGG 4 cut(s) 20, 426, 482, 510
HphI GGTGA 1 cut(s) 629
Hpy188I TCNGA 1 cut(s) 549
Hpy188III TCNNGA 2 cut(s) 482, 510
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 2 cut(s) 355, 517
HpyCH4IV ACGT 1 cut(s) 551
HpyCH4V TGCA 7 cut(s) 4, 167, 223, 332, 351, 389, 505
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 401
HpyF3I CTNAG 1 cut(s) 139
HpySE526I ACGT 1 cut(s) 551
Hsp92II CATG 3 cut(s) 103, 155, 227
Kpn2I TCCGGA 2 cut(s) 481, 509
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 2 cut(s) 22, 382
LpnPI CCDG 8 cut(s) 33, 126, 342, 361, 377, 439, 495, 523
Lsp1109I GCAGC 5 cut(s) 344, 361, 374, 388, 391
LweI GCATC 1 cut(s) 492
MaeI CTAG 1 cut(s) 422
MaeII ACGT 1 cut(s) 551
MaeIII GTNAC 3 cut(s) 15, 95, 304
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 4 cut(s) 133, 227, 239, 467
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 9 cut(s) 54, 84, 116, 230, 465, 492, 533, 543, 609
MluNI TGGCCA 1 cut(s) 395
MlyI GAGTC 3 cut(s) 38, 156, 433
MmeI TCCRAC 1 cut(s) 628
MnlI CCTC 5 cut(s) 74, 96, 148, 264, 607
Mox20I TGGCCA 1 cut(s) 395
MroI TCCGGA 2 cut(s) 481, 509
MscI TGGCCA 1 cut(s) 395
MseI TTAA 3 cut(s) 192, 296, 528
MslI CAYNNNNRTG 1 cut(s) 356
Msp20I TGGCCA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 4 cut(s) 20, 426, 482, 510
MvnI CGCG 1 cut(s) 208
MwoI GCNNNNNNNGC 2 cut(s) 371, 401
NdeII GATC 1 cut(s) 154
NlaIII CATG 3 cut(s) 103, 155, 227
NlaIV GGNNCC 2 cut(s) 135, 136
NmuCI GTSAC 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 356
PfeI GAWTC 1 cut(s) 49
PflFI GACNNNGTC 1 cut(s) 442
PkrI GCNGC 6 cut(s) 210, 334, 364, 376, 403, 406
PleI GAGTC 3 cut(s) 37, 155, 433
PpsI GAGTC 3 cut(s) 37, 155, 433
PspEI GGTNACC 1 cut(s) 15
PspN4I GGNNCC 2 cut(s) 135, 136
PspOMI GGGCCC 1 cut(s) 134
PspPI GGNCC 3 cut(s) 134, 135, 326
PstI CTGCAG 1 cut(s) 334
PsyI GACNNNGTC 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 356
SaqAI TTAA 3 cut(s) 192, 296, 528
SatI GCNGC 6 cut(s) 209, 333, 363, 375, 402, 405
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 3 cut(s) 134, 135, 326
SchI GAGTC 3 cut(s) 38, 156, 433
SduI GDGCHC 1 cut(s) 138
SetI ASST 7 cut(s) 11, 66, 145, 192, 337, 554, 659
SfaNI GCATC 1 cut(s) 492
SfcI CTRYAG 1 cut(s) 330
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 356
Sse9I AATT 9 cut(s) 54, 84, 116, 230, 465, 492, 533, 543, 609
SsiI CCGC 1 cut(s) 208
SspMI CTAG 1 cut(s) 422
TaaI ACNGT 2 cut(s) 355, 517
TaiI ACGT 1 cut(s) 554
TaqI TCGA 2 cut(s) 183, 474
TasI AATT 9 cut(s) 54, 84, 116, 230, 465, 492, 533, 543, 609
TatI WGTACW 1 cut(s) 272
TauI GCSGC 1 cut(s) 211
TfiI GAWTC 1 cut(s) 49
Tru1I TTAA 3 cut(s) 192, 296, 528
Tru9I TTAA 3 cut(s) 192, 296, 528
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 5 cut(s) 332, 362, 374, 401, 404
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 4 cut(s) 51, 240, 310, 650
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 1 cut(s) 396
Tth111I GACNNNGTC 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 326
XapI RAATTY 2 cut(s) 465, 543
XcmI CCANNNNNNNNNTGG 1 cut(s) 532
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.