Rorug06G0000500

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004217
Physical Location & Seq
Reverse (-)
185619 .. 188090
2472 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0000500.1

Sequence Viewer

Length: 564 bp
ATGTTCACCAAAAGAATATGCTTGCTTGCTCAATCACAAGAGATTCAACAATTGAAAGAGATGGTTGGAGCTATTTTGGCACGGACAGAGAAAGGGGGGAATAACCATGGAAGTACCAGTGGTCAGCATTCTGGTAATCAGCTTTCACAACAAAAAGATAAGGAAAATACAAGTGTGAGCGTGCGAAATTCTGAAACTGAGAGACCACAAAGCAGGCACAGTAAACAAAAGAACTCAAGTGTTCAACATTCTGTTGATGAGGGGTTGAAGAGCATAGAAAAGGAAGGTGCAAGGACCAAGGGTATTAAGAATGATCATAATAGAATTGAGTTATTAAATTGGTTTCAGATGGGAGAAGAGGTTGTTGCAAGTGCAAAACTTGAGTCAAAGGATCCAAGTGCAATGGTCCATCACGTGCCTCTTGGCCACGAATGTTGGAAAGTTTGGGTTCTTGATGTTTTTGAGGATATAGCTTTGTATCGACCAACCAGTGAGTTTGGAAAACTGAGTATGGCTCAAGGAAGTACAATTGCATGGCCTATCAAATACATCAAATTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

187

Amino Acids

21.01

Weight (kDa)

8.83

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 118 - 186 1.1e-07 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 386, 399
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 1 cut(s) 187
AcvI CACGTG 1 cut(s) 415
AfaI GTAC 2 cut(s) 115, 526
AgsI TTSAA 4 cut(s) 47, 55, 245, 268
AluBI AGCT 3 cut(s) 71, 142, 473
AluI AGCT 3 cut(s) 71, 142, 473
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 2 cut(s) 386, 399
AoxI GGCC 2 cut(s) 424, 536
ApoI RAATTY 1 cut(s) 187
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AspS9I GGNCC 2 cut(s) 294, 406
AvaII GGWCC 2 cut(s) 294, 406
BalI TGGCCA 1 cut(s) 426
BamHI GGATCC 1 cut(s) 391
BbrPI CACGTG 1 cut(s) 415
BccI CCATC 3 cut(s) 55, 343, 417
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 196
Bme18I GGWCC 2 cut(s) 294, 406
BmgT120I GGNCC 2 cut(s) 294, 406
BmiI GGNNCC 1 cut(s) 393
BplI GAGNNNNNCTC 2 cut(s) 499, 531
BpuEI CTTGAG 3 cut(s) 220, 401, 501
BsaAI YACGTR 1 cut(s) 415
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 106, 297
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bse1I ACTGG 2 cut(s) 117, 489
Bse3DI GCAATG 1 cut(s) 408
BseDI CCNNGG 2 cut(s) 106, 297
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 2 cut(s) 189, 497
BseNI ACTGG 2 cut(s) 117, 489
BshFI GGCC 2 cut(s) 426, 538
BsmAI GTCTC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 127
BsnI GGCC 2 cut(s) 426, 538
Bso31I GGTCTC 1 cut(s) 196
Bsp143I GATC 2 cut(s) 313, 391
Bsp19I CCATGG 1 cut(s) 106
BspANI GGCC 2 cut(s) 426, 538
BspCNI CTCAG 2 cut(s) 190, 498
BspLI GGNNCC 1 cut(s) 393
BspPI GGATC 2 cut(s) 386, 399
BspQI GCTCTTC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 2 cut(s) 117, 489
BssECI CCNNGG 2 cut(s) 106, 297
BssMI GATC 2 cut(s) 313, 391
BssT1I CCWWGG 2 cut(s) 106, 297
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 2 cut(s) 263, 351
BstBAI YACGTR 1 cut(s) 415
BstC8I GCNNGC 4 cut(s) 23, 27, 182, 215
BstDEI CTNAG 2 cut(s) 198, 506
BstDSI CCRYGG 1 cut(s) 106
BstKTI GATC 2 cut(s) 316, 394
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 2 cut(s) 313, 391
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 2 cut(s) 426, 538
BtgI CCRYGG 1 cut(s) 106
BtsIMutI CAGTG 2 cut(s) 124, 496
Cac8I GCNNGC 4 cut(s) 23, 27, 182, 215
Cfr13I GGNCC 2 cut(s) 294, 406
Csp6I GTAC 2 cut(s) 114, 525
CspCI CAANNNNNGTGG 2 cut(s) 416, 451
CviAII CATG 2 cut(s) 107, 534
CviJI RGCY 6 cut(s) 71, 142, 426, 473, 515, 538
CviKI_1 RGCY 6 cut(s) 71, 142, 426, 473, 515, 538
CviQI GTAC 2 cut(s) 114, 525
DdeI CTNAG 2 cut(s) 198, 506
DpnI GATC 2 cut(s) 315, 393
DpnII GATC 2 cut(s) 313, 391
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 2 cut(s) 263, 351
EarI CTCTTC 2 cut(s) 263, 351
Eco130I CCWWGG 2 cut(s) 106, 297
Eco31I GGTCTC 1 cut(s) 196
Eco47I GGWCC 2 cut(s) 294, 406
Eco72I CACGTG 1 cut(s) 415
EcoT14I CCWWGG 2 cut(s) 106, 297
ErhI CCWWGG 2 cut(s) 106, 297
FaeI CATG 2 cut(s) 110, 537
FaiI YATR 7 cut(s) 19, 108, 275, 318, 470, 512, 535
FatI CATG 2 cut(s) 106, 533
FbaI TGATCA 1 cut(s) 313
HaeIII GGCC 2 cut(s) 426, 538
Hin1II CATG 2 cut(s) 110, 537
HinfI GANTC 2 cut(s) 43, 383
Hpy166II GTNNAC 2 cut(s) 6, 224
Hpy188I TCNGA 2 cut(s) 193, 348
Hpy188III TCNNGA 1 cut(s) 452
Hpy8I GTNNAC 2 cut(s) 6, 224
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 5 cut(s) 290, 368, 374, 401, 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 2 cut(s) 198, 506
HpySE526I ACGT 1 cut(s) 414
Hsp92II CATG 2 cut(s) 110, 537
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 2 cut(s) 313, 391
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 4 cut(s) 117, 130, 199, 502
MaeII ACGT 1 cut(s) 414
MalI GATC 2 cut(s) 315, 393
MboI GATC 2 cut(s) 313, 391
MboII GAAGA 2 cut(s) 280, 368
MfeI CAATTG 2 cut(s) 50, 528
MflI RGATCY 1 cut(s) 391
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 6 cut(s) 50, 187, 324, 337, 528, 554
MluNI TGGCCA 1 cut(s) 426
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 2 cut(s) 46, 416
MnlI CCTC 4 cut(s) 253, 352, 429, 457
Mox20I TGGCCA 1 cut(s) 426
MscI TGGCCA 1 cut(s) 426
MseI TTAA 2 cut(s) 306, 335
Msp20I TGGCCA 1 cut(s) 426
MunI CAATTG 2 cut(s) 50, 528
Mva1269I GAATGC 1 cut(s) 127
MwoI GCNNNNNNNGC 1 cut(s) 77
NcoI CCATGG 1 cut(s) 106
NdeII GATC 2 cut(s) 313, 391
NlaIII CATG 2 cut(s) 110, 537
NlaIV GGNNCC 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 263
PctI GAATGC 1 cut(s) 127
PfeI GAWTC 1 cut(s) 43
PleI GAGTC 1 cut(s) 391
PmaCI CACGTG 1 cut(s) 415
PmlI CACGTG 1 cut(s) 415
PpsI GAGTC 1 cut(s) 391
Ppu21I YACGTR 1 cut(s) 415
PspCI CACGTG 1 cut(s) 415
PspN4I GGNNCC 1 cut(s) 393
PspPI GGNCC 2 cut(s) 294, 406
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 2 cut(s) 115, 526
RsaNI GTAC 2 cut(s) 114, 525
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 2 cut(s) 306, 335
Sau3AI GATC 2 cut(s) 313, 391
Sau96I GGNCC 2 cut(s) 294, 406
SchI GAGTC 1 cut(s) 392
SetI ASST 6 cut(s) 73, 144, 289, 363, 417, 475
SinI GGWCC 2 cut(s) 294, 406
SmlI CTYRAG 3 cut(s) 235, 380, 516
SmoI CTYRAG 3 cut(s) 235, 380, 516
Sse9I AATT 6 cut(s) 50, 187, 324, 337, 528, 554
StyI CCWWGG 2 cut(s) 106, 297
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 1 cut(s) 417
TaqI TCGA 1 cut(s) 481
TasI AATT 6 cut(s) 50, 187, 324, 337, 528, 554
TatI WGTACW 1 cut(s) 524
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 2 cut(s) 306, 335
Tru9I TTAA 2 cut(s) 306, 335
TscAI CASTG 2 cut(s) 124, 496
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 2 cut(s) 124, 496
VpaK11BI GGWCC 2 cut(s) 294, 406
XapI RAATTY 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.