RchiOBHm_Chr6g0262601

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004217
Physical Location & Seq
Forward (+)
17404668 .. 17406199
1532 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23550

Sequence Viewer

Length: 528 bp
ATGAAGGGAGTGAGAAAGTTAAGTCGAAAGATCTTTAAAAAGATTTGCAAACGTGATGGAGATCTTTGGAGATATTACGAGGACGCTTGCAAGAATGAAGAAGATTTCCTAACTGAACTGGGGAAGTTCCTAGAGTCGTATGAAAAATGTTTGATTCAAATTACGAAGGTGGGAAACAACGACGGAGGAATTGATGATGGTTACATGAGGATTTTGGAGAAATTGAAGAATGTCAAGGGGGCCTCAGGTAGAGTCCATGATCAGCATTTTGCGGAACAAATGTTGCTCGAAAATGTTAAATGTTTGATGAAGCGGCGAGAAGATTTGCATGAAGAATTGATTAGCCAAAAAGGAAAGCCTCGCAAGAAGTATGATTGTACTCGTGCTTGGAGACGCTGTTTGAGGATGGTCACTAATATATTGTTCATTGGTGCTGCAGCTGCTGAGTTTGCCTGCACAGTTGTGGCAGCAGTTATGGCTGCTCCACTTGTTGCACTGGCCGTTGCTGCTACTATTATCCAACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

175

Amino Acids

20.01

Weight (kDa)

9.24

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 59 - 170 2.7e-08 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 272, 313
AcoI YGGCCR 1 cut(s) 498
AfaI GTAC 1 cut(s) 379
AgsI TTSAA 2 cut(s) 158, 226
AleI CACNNNNGTG 1 cut(s) 461
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw26I GTCTC 1 cut(s) 385
AlwNI CAGNNNCTG 1 cut(s) 443
AoxI GGCC 2 cut(s) 240, 498
ApeKI GCWGC 6 cut(s) 434, 437, 440, 467, 479, 506
AspS9I GGNCC 1 cut(s) 240
AxyI CCTNAGG 1 cut(s) 244
BauI CACGAG 1 cut(s) 381
BbvI GCAGC 6 cut(s) 421, 427, 449, 466, 479, 493
BccI CCATC 3 cut(s) 50, 191, 400
BceAI ACGGC 1 cut(s) 485
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 385
BfaI CTAG 2 cut(s) 131, 526
BfmI CTRYAG 1 cut(s) 435
BglII AGATCT 2 cut(s) 30, 61
BisI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
BlsI GCNGC 7 cut(s) 315, 436, 439, 442, 469, 481, 508
BmgT120I GGNCC 1 cut(s) 240
BmiI GGNNCC 1 cut(s) 241
BmrI ACTGGG 1 cut(s) 128
BmuI ACTGGG 1 cut(s) 128
BsaBI GATNNNNATC 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bse1I ACTGG 2 cut(s) 123, 501
Bse21I CCTNAGG 1 cut(s) 244
Bse8I GATNNNNATC 1 cut(s) 60
BseGI GGATG 1 cut(s) 411
BseJI GATNNNNATC 1 cut(s) 60
BseMII CTCAG 2 cut(s) 258, 435
BseNI ACTGG 2 cut(s) 123, 501
BseXI GCAGC 6 cut(s) 421, 427, 449, 466, 479, 493
BsgI GTGCAG 1 cut(s) 439
BshFI GGCC 2 cut(s) 242, 500
BsmAI GTCTC 1 cut(s) 385
BsmBI CGTCTC 1 cut(s) 385
BsnI GGCC 2 cut(s) 242, 500
Bsp143I GATC 3 cut(s) 30, 61, 259
BspACI CCGC 2 cut(s) 272, 313
BspANI GGCC 2 cut(s) 242, 500
BspCNI CTCAG 2 cut(s) 257, 436
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 439
BsrI ACTGG 2 cut(s) 123, 501
BssMI GATC 3 cut(s) 30, 61, 259
BssSI CACGAG 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 88, 454
BstDEI CTNAG 2 cut(s) 244, 444
BstF5I GGATG 1 cut(s) 411
BstKTI GATC 3 cut(s) 33, 64, 262
BstMAI GTCTC 1 cut(s) 385
BstMBI GATC 3 cut(s) 30, 61, 259
BstMWI GCNNNNNNNGC 4 cut(s) 440, 449, 476, 506
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 6 cut(s) 421, 427, 449, 466, 479, 493
BstX2I RGATCY 2 cut(s) 30, 61
BstYI RGATCY 2 cut(s) 30, 61
Bsu36I CCTNAGG 1 cut(s) 244
BsuRI GGCC 2 cut(s) 242, 500
BtsCI GGATG 1 cut(s) 411
BtsIMutI CAGTG 1 cut(s) 494
Cac8I GCNNGC 2 cut(s) 88, 454
CaiI CAGNNNCTG 1 cut(s) 443
Cfr13I GGNCC 1 cut(s) 240
CseI GACGC 2 cut(s) 92, 402
Csp6I GTAC 1 cut(s) 378
CviAII CATG 3 cut(s) 205, 257, 329
CviJI RGCY 6 cut(s) 242, 345, 358, 440, 479, 500
CviKI_1 RGCY 6 cut(s) 242, 345, 358, 440, 479, 500
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 2 cut(s) 244, 444
DpnI GATC 3 cut(s) 32, 63, 261
DpnII GATC 3 cut(s) 30, 61, 259
DraI TTTAAA 1 cut(s) 37
EaeI YGGCCR 1 cut(s) 498
Eco81I CCTNAGG 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 385
FaeI CATG 3 cut(s) 208, 260, 332
FaiI YATR 7 cut(s) 141, 206, 258, 330, 372, 419, 476
FatI CATG 3 cut(s) 204, 256, 328
FbaI TGATCA 1 cut(s) 259
Fnu4HI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
FokI GGATG 1 cut(s) 418
Fsp4HI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
FspBI CTAG 2 cut(s) 131, 526
GluI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
HaeIII GGCC 2 cut(s) 242, 500
HgaI GACGC 2 cut(s) 92, 402
Hin1II CATG 3 cut(s) 208, 260, 332
HinfI GANTC 3 cut(s) 134, 154, 252
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 6 cut(s) 48, 90, 328, 437, 456, 494
HpyF10VI GCNNNNNNNGC 4 cut(s) 440, 449, 476, 506
HpyF3I CTNAG 2 cut(s) 244, 444
HpySE526I ACGT 1 cut(s) 52
Hsp92II CATG 3 cut(s) 208, 260, 332
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 3 cut(s) 30, 61, 259
LmnI GCTCC 1 cut(s) 487
LpnPI CCDG 4 cut(s) 104, 231, 466, 482
Lsp1109I GCAGC 6 cut(s) 421, 427, 449, 466, 479, 493
MaeI CTAG 2 cut(s) 131, 526
MaeII ACGT 1 cut(s) 52
MaeIII GTNAC 2 cut(s) 200, 409
MalI GATC 3 cut(s) 32, 63, 261
MboI GATC 3 cut(s) 30, 61, 259
MboII GAAGA 5 cut(s) 110, 113, 238, 332, 344
MflI RGATCY 2 cut(s) 30, 61
MluCI AATT 4 cut(s) 159, 189, 221, 335
MlyI GAGTC 2 cut(s) 143, 261
MnlI CCTC 6 cut(s) 73, 179, 201, 253, 369, 396
MseI TTAA 3 cut(s) 20, 36, 297
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 440
MwoI GCNNNNNNNGC 4 cut(s) 440, 449, 476, 506
NdeII GATC 3 cut(s) 30, 61, 259
NlaIII CATG 3 cut(s) 208, 260, 332
NlaIV GGNNCC 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 409
OliI CACNNNNGTG 1 cut(s) 461
PfeI GAWTC 1 cut(s) 154
PkrI GCNGC 7 cut(s) 315, 436, 439, 442, 469, 481, 508
PleI GAGTC 2 cut(s) 142, 260
PpsI GAGTC 2 cut(s) 142, 260
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 1 cut(s) 240
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 2 cut(s) 30, 61
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 3 cut(s) 20, 36, 297
SatI GCNGC 7 cut(s) 314, 435, 438, 441, 468, 480, 507
Sau3AI GATC 3 cut(s) 30, 61, 259
Sau96I GGNCC 1 cut(s) 240
SchI GAGTC 2 cut(s) 143, 261
SetI ASST 4 cut(s) 55, 171, 250, 442
SfcI CTRYAG 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 461
Sse9I AATT 4 cut(s) 159, 189, 221, 335
SsiI CCGC 2 cut(s) 272, 313
SspMI CTAG 2 cut(s) 131, 526
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 1 cut(s) 55
TaqI TCGA 2 cut(s) 25, 288
TasI AATT 4 cut(s) 159, 189, 221, 335
TatI WGTACW 1 cut(s) 377
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 3 cut(s) 20, 36, 297
Tru9I TTAA 3 cut(s) 20, 36, 297
TscAI CASTG 1 cut(s) 501
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 6 cut(s) 434, 437, 440, 467, 479, 506
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 6 cut(s) 17, 111, 156, 323, 345, 415
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 1 cut(s) 501
XspI CTAG 2 cut(s) 131, 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.