Rh6CG112300

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004217
Physical Location & Seq
Forward (+)
12902077 .. 12902922
846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG112300.1

Sequence Viewer

Length: 846 bp
ATGGGAATGAAGGGAGTGAGAAAGTTAAGTCGAAAGATCCTCAAAAACGTTTGCAAACGTGAGGAAGATCTCTGGAGATCATACGAGGACGCTTGCAAGAATGAAAAGGATTTCCTAACTGAACTGGACAAGTTCCTAGAGTCGTATAAGAAATGTTTGATTCAAATTAATGACGACGGAGGAATTGATGATGGTTACGTGAGGATTTTGGAGAAATTGAAAAACGTCAAGGGGGCCTCAGGTAGAGTCTATGATCGGCAATTTGCAGATCAAATTTTGCTCAAAAAGGTTGAATGTTTGGTGACGCAGCGAGAACATTTGCAAAGAGAATTGAATGACCGACTACAAAAGCATCGCAAGAAGTATGATTGTACTCGTGCTTGGAGACGCTGTTTGAGGACGGTCACTCATATATTGTTCATTGGTGCTGCAGCTGCTGAGTTTGCCTGCACAGTTGTGGCCGCAGTTATGGCTGCTCCACTTGTTGCACTGGCCATTGCTGCTGCTATTATCCCAACACTAGCCGGACAGCATTGGACTCTGTCTTGGATTGAAGATTCTGAAATTCCAGAGTATTCTGACTCTGTAATTCGCTTAATGCAATCCAGAACGAACGAAGCCATTATGGATTTGCAAGTAATTCCGACGGATCTCCGTACTGTTGTAGGGGACATAGAATCACTCTTATCCCATCTCAATTCTGTAATTGAGGAAGATGAAGATATCGATATGAAAATTATTGAGGGTCTTCTGAAGGACAGTAATGAGTTGAAGGAAAGAGCAGGGAATACCAAGTCCAAGATATTGGAGGCAAGCGATGAGGTTTCTAAGAAGATAAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

281

Amino Acids

32.05

Weight (kDa)

6.28

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 60 - 262 2e-13 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 462
AclI AACGTT 1 cut(s) 48
AclWI GGATC 2 cut(s) 31, 657
AcoI YGGCCR 2 cut(s) 459, 492
AcsI RAATTY 2 cut(s) 273, 564
AcuI CTGAAG 1 cut(s) 773
AfaI GTAC 2 cut(s) 373, 658
AgsI TTSAA 6 cut(s) 164, 220, 293, 334, 554, 772
AleI CACNNNNGTG 1 cut(s) 455
AluBI AGCT 1 cut(s) 434
AluI AGCT 1 cut(s) 434
Alw26I GTCTC 1 cut(s) 379
AlwI GGATC 2 cut(s) 31, 657
AlwNI CAGNNNCTG 1 cut(s) 437
AoxI GGCC 3 cut(s) 234, 459, 492
ApeKI GCWGC 7 cut(s) 307, 428, 431, 434, 473, 500, 503
ApoI RAATTY 2 cut(s) 273, 564
AseI ATTAAT 1 cut(s) 168
AspS9I GGNCC 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 313
AxyI CCTNAGG 1 cut(s) 238
BalI TGGCCA 1 cut(s) 494
BauI CACGAG 1 cut(s) 375
BbsI GAAGAC 1 cut(s) 740
BbvI GCAGC 7 cut(s) 319, 415, 421, 443, 460, 487, 490
BccI CCATC 2 cut(s) 185, 699
BcoDI GTCTC 1 cut(s) 379
BfaI CTAG 2 cut(s) 137, 521
BfmI CTRYAG 1 cut(s) 429
BglII AGATCT 1 cut(s) 67
BisI GCNGC 8 cut(s) 308, 429, 432, 435, 462, 474, 501, 504
BlsI GCNGC 8 cut(s) 309, 430, 433, 436, 463, 475, 502, 505
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 1 cut(s) 235
BmsI GCATC 1 cut(s) 361
BpiI GAAGAC 1 cut(s) 740
BpmI CTGGAG 1 cut(s) 94
Bsa29I ATCGAT 1 cut(s) 726
BsaAI YACGTR 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 376, 406
Bse1I ACTGG 2 cut(s) 129, 495
Bse21I CCTNAGG 1 cut(s) 238
Bse3DI GCAATG 1 cut(s) 495
BseCI ATCGAT 1 cut(s) 726
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 2 cut(s) 252, 429
BseNI ACTGG 2 cut(s) 129, 495
BseXI GCAGC 7 cut(s) 319, 415, 421, 443, 460, 487, 490
BsgI GTGCAG 1 cut(s) 433
BshFI GGCC 3 cut(s) 236, 461, 494
BshVI ATCGAT 1 cut(s) 726
BsiSI CCGG 1 cut(s) 525
BslFI GGGAC 1 cut(s) 683
BsmAI GTCTC 1 cut(s) 379
BsmBI CGTCTC 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 683
BsnI GGCC 3 cut(s) 236, 461, 494
Bsp143I GATC 6 cut(s) 36, 67, 77, 253, 268, 649
BspACI CCGC 1 cut(s) 462
BspANI GGCC 3 cut(s) 236, 461, 494
BspCNI CTCAG 2 cut(s) 251, 430
BspDI ATCGAT 1 cut(s) 726
BspLI GGNNCC 1 cut(s) 235
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 2 cut(s) 31, 657
BsrDI GCAATG 1 cut(s) 495
BsrI ACTGG 2 cut(s) 129, 495
BssMI GATC 6 cut(s) 36, 67, 77, 253, 268, 649
BssSI CACGAG 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 375
Bst4CI ACNGT 4 cut(s) 403, 454, 661, 761
BstBAI YACGTR 1 cut(s) 199
BstC8I GCNNGC 3 cut(s) 94, 448, 814
BstDEI CTNAG 3 cut(s) 238, 438, 828
BstKTI GATC 6 cut(s) 39, 70, 80, 256, 271, 652
BstMAI GTCTC 1 cut(s) 379
BstMBI GATC 6 cut(s) 36, 67, 77, 253, 268, 649
BstMWI GCNNNNNNNGC 4 cut(s) 434, 443, 470, 500
BstSFI CTRYAG 1 cut(s) 429
BstV1I GCAGC 7 cut(s) 319, 415, 421, 443, 460, 487, 490
BstV2I GAAGAC 1 cut(s) 740
BstX2I RGATCY 3 cut(s) 36, 67, 649
BstXI CCANNNNNNTGG 1 cut(s) 805
BstYI RGATCY 3 cut(s) 36, 67, 649
Bsu15I ATCGAT 1 cut(s) 726
Bsu36I CCTNAGG 1 cut(s) 238
BsuRI GGCC 3 cut(s) 236, 461, 494
BsuTUI ATCGAT 1 cut(s) 726
BtgZI GCGATG 2 cut(s) 338, 831
BtsIMutI CAGTG 1 cut(s) 488
Cac8I GCNNGC 3 cut(s) 94, 448, 814
CaiI CAGNNNCTG 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 234
ClaI ATCGAT 1 cut(s) 726
CseI GACGC 3 cut(s) 98, 313, 396
Csp6I GTAC 2 cut(s) 372, 657
CviJI RGCY 7 cut(s) 236, 434, 461, 473, 494, 524, 620
CviKI_1 RGCY 7 cut(s) 236, 434, 461, 473, 494, 524, 620
CviQI GTAC 2 cut(s) 372, 657
DdeI CTNAG 3 cut(s) 238, 438, 828
DpnI GATC 6 cut(s) 38, 69, 79, 255, 270, 651
DpnII GATC 6 cut(s) 36, 67, 77, 253, 268, 649
EaeI YGGCCR 2 cut(s) 459, 492
Eco32I GATATC 1 cut(s) 724
Eco57I CTGAAG 1 cut(s) 773
Eco81I CCTNAGG 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 234
EcoRV GATATC 1 cut(s) 724
Esp3I CGTCTC 1 cut(s) 379
FaqI GGGAC 1 cut(s) 683
Fnu4HI GCNGC 8 cut(s) 308, 429, 432, 435, 462, 474, 501, 504
Fsp4HI GCNGC 8 cut(s) 308, 429, 432, 435, 462, 474, 501, 504
FspBI CTAG 2 cut(s) 137, 521
GluI GCNGC 8 cut(s) 308, 429, 432, 435, 462, 474, 501, 504
GsuI CTGGAG 1 cut(s) 94
HaeIII GGCC 3 cut(s) 236, 461, 494
HapII CCGG 1 cut(s) 525
HgaI GACGC 3 cut(s) 98, 313, 396
HinfI GANTC 7 cut(s) 140, 160, 246, 538, 557, 581, 677
HpaII CCGG 1 cut(s) 525
HphI GGTGA 1 cut(s) 313
Hpy188I TCNGA 4 cut(s) 562, 580, 645, 753
Hpy188III TCNNGA 3 cut(s) 73, 569, 606
Hpy99I CGWCG 2 cut(s) 179, 649
HpyAV CCTTC 3 cut(s) 4, 748, 766
HpyCH4III ACNGT 4 cut(s) 403, 454, 661, 761
HpyCH4IV ACGT 4 cut(s) 48, 58, 198, 225
HpyCH4V TGCA 9 cut(s) 54, 96, 266, 322, 431, 450, 488, 601, 634
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 443, 470, 500
HpyF3I CTNAG 3 cut(s) 238, 438, 828
HpySE526I ACGT 4 cut(s) 48, 58, 198, 225
Kzo9I GATC 6 cut(s) 36, 67, 77, 253, 268, 649
LmnI GCTCC 1 cut(s) 481
LpnPI CCDG 9 cut(s) 58, 110, 225, 460, 476, 538, 582, 619, 768
Lsp1109I GCAGC 7 cut(s) 319, 415, 421, 443, 460, 487, 490
LweI GCATC 1 cut(s) 361
MaeI CTAG 2 cut(s) 137, 521
MaeII ACGT 4 cut(s) 48, 58, 198, 225
MaeIII GTNAC 3 cut(s) 194, 301, 403
MalI GATC 6 cut(s) 38, 69, 79, 255, 270, 651
MboI GATC 6 cut(s) 36, 67, 77, 253, 268, 649
MboII GAAGA 6 cut(s) 77, 566, 725, 731, 740, 844
MflI RGATCY 3 cut(s) 36, 67, 649
MlsI TGGCCA 1 cut(s) 494
MluNI TGGCCA 1 cut(s) 494
MlyI GAGTC 4 cut(s) 149, 255, 532, 575
MmeI TCCRAC 1 cut(s) 668
Mox20I TGGCCA 1 cut(s) 494
MscI TGGCCA 1 cut(s) 494
MseI TTAA 3 cut(s) 26, 168, 596
MslI CAYNNNNRTG 1 cut(s) 455
Msp20I TGGCCA 1 cut(s) 494
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 1 cut(s) 525
MwoI GCNNNNNNNGC 4 cut(s) 434, 443, 470, 500
NdeII GATC 6 cut(s) 36, 67, 77, 253, 268, 649
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 2 cut(s) 301, 403
OliI CACNNNNGTG 1 cut(s) 455
PfeI GAWTC 3 cut(s) 160, 557, 677
PflFI GACNNNGTC 1 cut(s) 541
PkrI GCNGC 8 cut(s) 309, 430, 433, 436, 463, 475, 502, 505
PleI GAGTC 4 cut(s) 148, 254, 532, 575
PpsI GAGTC 4 cut(s) 148, 254, 532, 575
Ppu21I YACGTR 1 cut(s) 199
PshBI ATTAAT 1 cut(s) 168
Psp1406I AACGTT 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 234
PstI CTGCAG 1 cut(s) 433
PstNI CAGNNNCTG 1 cut(s) 437
PsuI RGATCY 3 cut(s) 36, 67, 649
PsyI GACNNNGTC 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 2 cut(s) 373, 658
RsaNI GTAC 2 cut(s) 372, 657
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 3 cut(s) 26, 168, 596
SatI GCNGC 8 cut(s) 308, 429, 432, 435, 462, 474, 501, 504
Sau3AI GATC 6 cut(s) 36, 67, 77, 253, 268, 649
Sau96I GGNCC 1 cut(s) 234
SchI GAGTC 4 cut(s) 149, 255, 532, 575
SetI ASST 8 cut(s) 51, 61, 201, 228, 244, 291, 436, 825
SfaNI GCATC 1 cut(s) 361
SfcI CTRYAG 1 cut(s) 429
SmiMI CAYNNNNRTG 1 cut(s) 455
SsiI CCGC 1 cut(s) 462
SspMI CTAG 2 cut(s) 137, 521
TaaI ACNGT 4 cut(s) 403, 454, 661, 761
TaiI ACGT 4 cut(s) 51, 61, 201, 228
TaqI TCGA 2 cut(s) 31, 726
TaqII GACCGA 1 cut(s) 354
TatI WGTACW 1 cut(s) 371
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 3 cut(s) 160, 557, 677
Tru1I TTAA 3 cut(s) 26, 168, 596
Tru9I TTAA 3 cut(s) 26, 168, 596
TscAI CASTG 1 cut(s) 495
TseFI GTSAC 2 cut(s) 301, 403
TseI GCWGC 7 cut(s) 307, 428, 431, 434, 473, 500, 503
Tsp45I GTSAC 2 cut(s) 301, 403
TspDTI ATGAA 5 cut(s) 23, 117, 409, 732, 746
TspGWI ACGGA 3 cut(s) 192, 644, 662
TspRI CASTG 1 cut(s) 495
Tth111I GACNNNGTC 1 cut(s) 541
VspI ATTAAT 1 cut(s) 168
XapI RAATTY 2 cut(s) 273, 564
XspI CTAG 2 cut(s) 137, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.