RLG00000014415

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004217
Physical Location & Seq
Reverse (-)
52918783 .. 52919667
885 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014415

Sequence Viewer

Length: 885 bp
ATGGGAATGAAGGGAGTGAGAAAGTTAAGTCGAAAGATCTTTAAAAAGATTTGCAAACGTGACGGAGATCTTTGGAGATCTTATGAGGACGCTTGCAAGAATGAAGAAGATTTCCTAACTGAACTGGGGAAGTTCCTAGAGTTGTATGAAAAATGTTTGATTCAAATTACGAAGGTGGGAAACAACGACGGAGGAATTGATGATGGTTACATGAGGATTTTGGAGAAATTGAAGAATGTCAAGGGGGCCTCAGGTAGAGTCCATGATCAGCATTTTGCGGTACAAATGTTGCTCGAAAATGTTAAATGTTTGATGAAGCGGCGAGAAGATTTGCATGACGAATTGATTAGCCAAAAAGGAAAGCCTCGCAAGAAGTATGATTGTACTCGTGCTTGGAGACGCTGTTTGAGGATGGTCACTAATATATTGTTCATTGGTGCTGCAGCTGCTGAGTTTGCCTGCACAGTTGTGGCAGCAGTTATGGCTGCTCCACTTGTTGCACTGGCCATTGCTGCTACTATTATCCCAACACTAGCCGGACAGCATTGGACTCTGTCTTGGATTGAGGATTGTGAAATTCCTTTCGAGTATCCTGATGATGTAATTGGCTTAATGCAATTCGGAACTGTGCTTTCCGTTAAGGAATTGGGAGAAATTCAGACGCGTCTCGATCGTGTTGTAAATGACATAAAATCACTCTTATCCCATCTGAACTCTGTAATTGAGGATGAAGTAAATCTCAATATGAAAAGTATTGAGCGTCTTCTGAAGAACATTAATGAATTGATGGAAAGAGCAAGGAATTCCAAGTCCCAGATATTAGAGGCAAGAGATGAGGTTTCTACGAGCATCAAGATCATGCACGAAGAGCATATAAAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

295

Amino Acids

33.52

Weight (kDa)

8.05

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 62 - 280 9.1e-17 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 664
AciI CCGC 2 cut(s) 278, 319
AcoI YGGCCR 1 cut(s) 504
AcsI RAATTY 3 cut(s) 576, 654, 802
AcuI CTGAAG 1 cut(s) 788
AfaI GTAC 2 cut(s) 282, 385
AflIII ACRYGT 1 cut(s) 662
AgsI TTSAA 2 cut(s) 164, 232
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 1 cut(s) 446
AluI AGCT 1 cut(s) 446
Alw26I GTCTC 2 cut(s) 391, 671
AlwNI CAGNNNCTG 1 cut(s) 449
AoxI GGCC 2 cut(s) 246, 504
ApeKI GCWGC 6 cut(s) 440, 443, 446, 473, 485, 512
ApoI RAATTY 3 cut(s) 576, 654, 802
AseI ATTAAT 1 cut(s) 777
AspS9I GGNCC 1 cut(s) 246
AxyI CCTNAGG 1 cut(s) 250
BaeI ACNNNNGTAYC 2 cut(s) 272, 305
BalI TGGCCA 1 cut(s) 506
BauI CACGAG 1 cut(s) 387
BbsI GAAGAC 1 cut(s) 755
BbvI GCAGC 6 cut(s) 427, 433, 455, 472, 485, 499
BccI CCATC 4 cut(s) 197, 406, 714, 781
BciVI GTATCC 1 cut(s) 600
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 391, 671
BfaI CTAG 2 cut(s) 137, 533
BfmI CTRYAG 1 cut(s) 441
BfuI GTATCC 1 cut(s) 600
BglII AGATCT 3 cut(s) 36, 67, 77
BisI GCNGC 7 cut(s) 320, 441, 444, 447, 474, 486, 513
BlsI GCNGC 7 cut(s) 321, 442, 445, 448, 475, 487, 514
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 247
BmrI ACTGGG 1 cut(s) 134
BmsI GCATC 1 cut(s) 858
BmuI ACTGGG 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 755
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
Bse1I ACTGG 2 cut(s) 129, 507
Bse21I CCTNAGG 1 cut(s) 250
Bse3DI GCAATG 1 cut(s) 507
BseGI GGATG 2 cut(s) 417, 733
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 2 cut(s) 264, 441
BseNI ACTGG 2 cut(s) 129, 507
BseXI GCAGC 6 cut(s) 427, 433, 455, 472, 485, 499
BsgI GTGCAG 1 cut(s) 445
Bsh1236I CGCG 1 cut(s) 664
Bsh1285I CGRYCG 1 cut(s) 673
BshFI GGCC 2 cut(s) 248, 506
BsiEI CGRYCG 1 cut(s) 673
BsiSI CCGG 1 cut(s) 537
BslFI GGGAC 1 cut(s) 796
BsmAI GTCTC 2 cut(s) 391, 671
BsmBI CGTCTC 2 cut(s) 391, 671
BsmFI GGGAC 1 cut(s) 796
BsnI GGCC 2 cut(s) 248, 506
Bsp143I GATC 6 cut(s) 36, 67, 77, 265, 670, 855
BspACI CCGC 2 cut(s) 278, 319
BspANI GGCC 2 cut(s) 248, 506
BspCNI CTCAG 2 cut(s) 263, 442
BspFNI CGCG 1 cut(s) 664
BspLI GGNNCC 1 cut(s) 247
BspMAI CTGCAG 1 cut(s) 445
BspQI GCTCTTC 1 cut(s) 861
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 2 cut(s) 129, 507
BssMI GATC 6 cut(s) 36, 67, 77, 265, 670, 855
BssSI CACGAG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 387
Bst4CI ACNGT 2 cut(s) 466, 628
Bst6I CTCTTC 1 cut(s) 861
BstC8I GCNNGC 2 cut(s) 94, 460
BstDEI CTNAG 2 cut(s) 250, 450
BstF5I GGATG 2 cut(s) 417, 733
BstFNI CGCG 1 cut(s) 664
BstKTI GATC 6 cut(s) 39, 70, 80, 268, 673, 858
BstMAI GTCTC 2 cut(s) 391, 671
BstMBI GATC 6 cut(s) 36, 67, 77, 265, 670, 855
BstMCI CGRYCG 1 cut(s) 673
BstMWI GCNNNNNNNGC 5 cut(s) 446, 455, 482, 512, 868
BstSFI CTRYAG 1 cut(s) 441
BstUI CGCG 1 cut(s) 664
BstV1I GCAGC 6 cut(s) 427, 433, 455, 472, 485, 499
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 3 cut(s) 36, 67, 77
BstYI RGATCY 3 cut(s) 36, 67, 77
Bsu36I CCTNAGG 1 cut(s) 250
BsuI GTATCC 1 cut(s) 600
BsuRI GGCC 2 cut(s) 248, 506
BtsCI GGATG 2 cut(s) 417, 733
BtsIMutI CAGTG 1 cut(s) 500
Cac8I GCNNGC 2 cut(s) 94, 460
CaiI CAGNNNCTG 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 246
CseI GACGC 5 cut(s) 98, 408, 653, 670, 749
Csp6I GTAC 2 cut(s) 281, 384
CviAII CATG 4 cut(s) 211, 263, 335, 859
CviJI RGCY 8 cut(s) 248, 351, 364, 446, 485, 506, 536, 609
CviKI_1 RGCY 8 cut(s) 248, 351, 364, 446, 485, 506, 536, 609
CviQI GTAC 2 cut(s) 281, 384
DdeI CTNAG 2 cut(s) 250, 450
DpnI GATC 6 cut(s) 38, 69, 79, 267, 672, 857
DpnII GATC 6 cut(s) 36, 67, 77, 265, 670, 855
DraI TTTAAA 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 504
Eam1104I CTCTTC 1 cut(s) 861
EarI CTCTTC 1 cut(s) 861
Eco57I CTGAAG 1 cut(s) 788
Eco81I CCTNAGG 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 246
EcoRI GAATTC 1 cut(s) 802
Esp3I CGTCTC 2 cut(s) 391, 671
FaeI CATG 4 cut(s) 214, 266, 338, 862
FaqI GGGAC 1 cut(s) 796
FatI CATG 4 cut(s) 210, 262, 334, 858
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 7 cut(s) 320, 441, 444, 447, 474, 486, 513
FokI GGATG 2 cut(s) 424, 740
Fsp4HI GCNGC 7 cut(s) 320, 441, 444, 447, 474, 486, 513
FspBI CTAG 2 cut(s) 137, 533
GluI GCNGC 7 cut(s) 320, 441, 444, 447, 474, 486, 513
HaeIII GGCC 2 cut(s) 248, 506
HapII CCGG 1 cut(s) 537
HgaI GACGC 5 cut(s) 98, 408, 653, 670, 749
Hin1II CATG 4 cut(s) 214, 266, 338, 862
HinfI GANTC 3 cut(s) 160, 258, 550
HpaII CCGG 1 cut(s) 537
Hpy188I TCNGA 4 cut(s) 623, 660, 711, 768
Hpy188III TCNNGA 3 cut(s) 593, 668, 853
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 4, 166
HpyCH4III ACNGT 2 cut(s) 466, 628
HpyCH4IV ACGT 1 cut(s) 58
HpyCH4V TGCA 8 cut(s) 54, 96, 334, 443, 462, 500, 616, 862
HpyF10VI GCNNNNNNNGC 5 cut(s) 446, 455, 482, 512, 868
HpyF3I CTNAG 2 cut(s) 250, 450
HpySE526I ACGT 1 cut(s) 58
Hsp92II CATG 4 cut(s) 214, 266, 338, 862
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 6 cut(s) 36, 67, 77, 265, 670, 855
LguI GCTCTTC 1 cut(s) 861
LmnI GCTCC 1 cut(s) 493
LpnPI CCDG 7 cut(s) 110, 237, 472, 488, 550, 606, 827
Lsp1109I GCAGC 6 cut(s) 427, 433, 455, 472, 485, 499
LweI GCATC 1 cut(s) 858
MaeI CTAG 2 cut(s) 137, 533
MaeII ACGT 1 cut(s) 58
MaeIII GTNAC 3 cut(s) 59, 206, 415
MalI GATC 6 cut(s) 38, 69, 79, 267, 672, 857
MboI GATC 6 cut(s) 36, 67, 77, 265, 670, 855
MboII GAAGA 7 cut(s) 116, 119, 244, 338, 755, 781, 878
MflI RGATCY 3 cut(s) 36, 67, 77
MlsI TGGCCA 1 cut(s) 506
MluI ACGCGT 1 cut(s) 662
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 2 cut(s) 267, 544
Mox20I TGGCCA 1 cut(s) 506
MscI TGGCCA 1 cut(s) 506
MseI TTAA 6 cut(s) 26, 42, 303, 611, 639, 777
MslI CAYNNNNRTG 1 cut(s) 467
Msp20I TGGCCA 1 cut(s) 506
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 1 cut(s) 537
MvnI CGCG 1 cut(s) 664
MwoI GCNNNNNNNGC 5 cut(s) 446, 455, 482, 512, 868
NdeII GATC 6 cut(s) 36, 67, 77, 265, 670, 855
NlaIII CATG 4 cut(s) 214, 266, 338, 862
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 2 cut(s) 59, 415
OliI CACNNNNGTG 1 cut(s) 467
PciSI GCTCTTC 1 cut(s) 861
PfeI GAWTC 1 cut(s) 160
PflFI GACNNNGTC 1 cut(s) 553
PkrI GCNGC 7 cut(s) 321, 442, 445, 448, 475, 487, 514
Ple19I CGATCG 1 cut(s) 673
PleI GAGTC 2 cut(s) 266, 544
PpsI GAGTC 2 cut(s) 266, 544
PshBI ATTAAT 1 cut(s) 777
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 246
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 3 cut(s) 36, 67, 77
PsyI GACNNNGTC 1 cut(s) 553
PvuI CGATCG 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 282, 385
RsaNI GTAC 2 cut(s) 281, 384
RseI CAYNNNNRTG 1 cut(s) 467
SapI GCTCTTC 1 cut(s) 861
SaqAI TTAA 6 cut(s) 26, 42, 303, 611, 639, 777
SatI GCNGC 7 cut(s) 320, 441, 444, 447, 474, 486, 513
Sau3AI GATC 6 cut(s) 36, 67, 77, 265, 670, 855
Sau96I GGNCC 1 cut(s) 246
SchI GAGTC 2 cut(s) 267, 544
SetI ASST 5 cut(s) 61, 177, 256, 448, 840
SfaNI GCATC 1 cut(s) 858
SfcI CTRYAG 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 467
SsiI CCGC 2 cut(s) 278, 319
SspMI CTAG 2 cut(s) 137, 533
TaaI ACNGT 2 cut(s) 466, 628
TaiI ACGT 1 cut(s) 61
TaqI TCGA 4 cut(s) 31, 294, 585, 669
TatI WGTACW 1 cut(s) 383
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 6 cut(s) 26, 42, 303, 611, 639, 777
Tru9I TTAA 6 cut(s) 26, 42, 303, 611, 639, 777
TscAI CASTG 1 cut(s) 507
TseFI GTSAC 2 cut(s) 59, 415
TseI GCWGC 6 cut(s) 440, 443, 446, 473, 485, 512
Tsp45I GTSAC 2 cut(s) 59, 415
TspDTI ATGAA 8 cut(s) 23, 117, 162, 329, 421, 744, 761, 795
TspGWI ACGGA 3 cut(s) 78, 204, 625
TspRI CASTG 1 cut(s) 507
Tth111I GACNNNGTC 1 cut(s) 553
VspI ATTAAT 1 cut(s) 777
XapI RAATTY 3 cut(s) 576, 654, 802
XspI CTAG 2 cut(s) 137, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.