Rorug06G0000400

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004217
Physical Location & Seq
Reverse (-)
167251 .. 167793
543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0000400.1

Sequence Viewer

Length: 543 bp
ATGGACTATCTTCAGTCCTTTGGTGGTGGTGAAGCTGATAGGGGATTTTGTGAAGGGGGCAGTGAGGATGAGGATTATGATTATGATCCTCAATTTGATGATGAGGATTATGACCACTATGGAACTGATGATGATGAATGGCTAGAAGAAGCAGTGATGGAGGTATCTCCAAAAGGCAAAACTGCAAGGGTGGGATCAAAGGGTCAAGAGTCAAATGTAGAGCAGGAAGTGGGGCACTTTTTTGATGATGAGGATATGTTTAGGGAGGATGGTTCAGATGATGAGAGGCTGCAATATGCCATTAATTCTGATGGAGAATTAGAGAATGTGAGGATTGAATTCAACCCTATTACTGATATGAAAAAACCCAAGTTTTGCTTGGGAATGAAGTTTGCTAATGTGAAGGTATTGAGGGATGCCTTAAGGGAGATGGCAATTCAGGGTGGATGGGAATATGTCTATATCAAGAATGATAAGACAAGGTTAAGGGTGGTGTGCAAGGAGGATAATTGCCTATTTTTTCTGTTTGACTCCAAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

180

Amino Acids

20.85

Weight (kDa)

4.24

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBD_Tnp_Mut PF03108 126 - 172 6.6e-09 MuDR family transposase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 80, 202
AcsI RAATTY 1 cut(s) 338
AflII CTTAAG 1 cut(s) 421
AgsI TTSAA 2 cut(s) 338, 343
AluBI AGCT 1 cut(s) 35
AluI AGCT 1 cut(s) 35
AlwI GGATC 2 cut(s) 80, 202
ApeKI GCWGC 1 cut(s) 289
ApoI RAATTY 1 cut(s) 338
AseI ATTAAT 1 cut(s) 303
AsuHPI GGTGA 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 276
BccI CCATC 5 cut(s) 151, 263, 305, 424, 441
BfaI CTAG 1 cut(s) 143
BfrI CTTAAG 1 cut(s) 421
BisI GCNGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 291
BmsI GCATC 1 cut(s) 406
BsaBI GATNNNNATC 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 84
BseGI GGATG 4 cut(s) 73, 274, 421, 452
BseJI GATNNNNATC 1 cut(s) 84
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 2 cut(s) 85, 194
BspPI GGATC 2 cut(s) 80, 202
BspTI CTTAAG 1 cut(s) 421
BssMI GATC 2 cut(s) 85, 194
BstAFI CTTAAG 1 cut(s) 421
BstF5I GGATG 4 cut(s) 73, 274, 421, 452
BstKTI GATC 2 cut(s) 88, 197
BstMBI GATC 2 cut(s) 85, 194
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 276
BtsCI GGATG 4 cut(s) 73, 274, 421, 452
BtsI GCAGTG 2 cut(s) 67, 159
BtsIMutI CAGTG 2 cut(s) 67, 159
CviJI RGCY 3 cut(s) 35, 142, 289
CviKI_1 RGCY 3 cut(s) 35, 142, 289
DpnI GATC 2 cut(s) 87, 196
DpnII GATC 2 cut(s) 85, 194
EcoRI GAATTC 1 cut(s) 338
FaiI YATR 9 cut(s) 78, 84, 111, 120, 257, 297, 359, 456, 462
FalI AAGNNNNNCTT 2 cut(s) 362, 394
Fnu4HI GCNGC 1 cut(s) 290
FokI GGATG 4 cut(s) 80, 281, 428, 459
Fsp4HI GCNGC 1 cut(s) 290
FspBI CTAG 1 cut(s) 143
GluI GCNGC 1 cut(s) 290
HinfI GANTC 2 cut(s) 209, 530
HphI GGTGA 1 cut(s) 41
Hpy188I TCNGA 2 cut(s) 277, 310
Hpy188III TCNNGA 2 cut(s) 206, 466
HpyAV CCTTC 2 cut(s) 47, 397
HpyCH4V TGCA 3 cut(s) 185, 292, 498
Kzo9I GATC 2 cut(s) 85, 194
LpnPI CCDG 2 cut(s) 209, 425
Lsp1109I GCAGC 1 cut(s) 276
LweI GCATC 1 cut(s) 406
MaeI CTAG 1 cut(s) 143
MalI GATC 2 cut(s) 87, 196
MboI GATC 2 cut(s) 85, 194
MboII GAAGA 1 cut(s) 158
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 6 cut(s) 92, 304, 317, 338, 435, 508
MlyI GAGTC 2 cut(s) 218, 524
MseI TTAA 3 cut(s) 303, 422, 485
MspCI CTTAAG 1 cut(s) 421
NdeII GATC 2 cut(s) 85, 194
PkrI GCNGC 1 cut(s) 291
PleI GAGTC 2 cut(s) 217, 524
PpsI GAGTC 2 cut(s) 217, 524
PshBI ATTAAT 1 cut(s) 303
SaqAI TTAA 3 cut(s) 303, 422, 485
SatI GCNGC 1 cut(s) 290
Sau3AI GATC 2 cut(s) 85, 194
SchI GAGTC 2 cut(s) 218, 524
SduI GDGCHC 1 cut(s) 237
SetI ASST 4 cut(s) 37, 165, 408, 485
SfaNI GCATC 1 cut(s) 406
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 6 cut(s) 92, 304, 317, 338, 435, 508
SspMI CTAG 1 cut(s) 143
TasI AATT 6 cut(s) 92, 304, 317, 338, 435, 508
Tru1I TTAA 3 cut(s) 303, 422, 485
Tru9I TTAA 3 cut(s) 303, 422, 485
TscAI CASTG 2 cut(s) 67, 159
TseI GCWGC 1 cut(s) 289
TspDTI ATGAA 3 cut(s) 150, 374, 401
TspRI CASTG 2 cut(s) 67, 159
Vha464I CTTAAG 1 cut(s) 421
VspI ATTAAT 1 cut(s) 303
XapI RAATTY 1 cut(s) 338
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XspI CTAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.