RLG00000014408

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004217
Physical Location & Seq
Reverse (-)
52832951 .. 52834999
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014408

Sequence Viewer

Length: 858 bp
ATGGGAATGAAGGGAGTGAGAAAGTTAAGTCGAAAGATCCTCAAAAACGTTTGCAAACGTGAGGGAGATCTCTGGAGATCATACGAGGACGCTTGCAAGAATGAAAAGGATTTCCTAACTGAACTGGACAAGTTCCTAGAGTCGTATAAGAAATGTTTGATTCAAATTAATGACGACGGGGGAATTGATGATGGTTACATGAGGATTTTGGAGAAATTGAAAAACGTCAAGGGGGCCTCAGGAAGAGTTTATGATCAGCAATTTGCAAATCAAATTTTGCTCAAAAAGGTTGAATGTCTGGCGACGCAGCGAGAAGATTTGCGAGAAGATTTGCGAAAAGAATTGAATGACCGACTACAAAAGCCTCGCAAGAAGTATGATTGTAGTCGTGCTTGGAGACGCTGTTTGAGGACGGTCACTCATATATTGTTCATTGGTGCTGCAGCTGCTGAGTTTGCCTGCACAGTTGTGGCCGCAGTTATGGCTGCTCCACTTGTTGCACTGGCCATTGCTGCTGCTATTATCCCAACACTAGCCGGACAGCATTGGACTGTGTCTTGGATTGAAGATTCTGAAATTCCTGAGTATTCTGACTCTGTGATTCGCTTAATGCAATCCGGAACTGAGACTTCCATTGTGAATTTGCAACATATTCAGACGTATCTCTCTGCTGTTGTAAGGGACATAGAATCACTCTTATCCCATCTCAATTCTGTAATTGAGGAAGATGAAGATATCAATATAAAAATTGTTGAGCGTCTTCTGAAGGACATTAATGAGTTGAAGGAAAGAGCAGGGAATAGCAAGTCCGAGATATTGGAGGCAAGCGATAAGGTTTCTAAGGAGATAAAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

286

Amino Acids

32.44

Weight (kDa)

6.49

Isoelectric Point (pI)

50.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 60 - 278 1.6e-15 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 617
AciI CCGC 1 cut(s) 474
AclI AACGTT 1 cut(s) 48
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 2 cut(s) 471, 504
AcsI RAATTY 3 cut(s) 273, 576, 640
AcuI CTGAAG 1 cut(s) 785
AgsI TTSAA 6 cut(s) 164, 220, 293, 346, 566, 784
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 1 cut(s) 446
AluI AGCT 1 cut(s) 446
Alw26I GTCTC 2 cut(s) 391, 620
AlwI GGATC 1 cut(s) 31
AlwNI CAGNNNCTG 1 cut(s) 449
Aor13HI TCCGGA 1 cut(s) 617
AoxI GGCC 3 cut(s) 234, 471, 504
ApeKI GCWGC 7 cut(s) 307, 440, 443, 446, 485, 512, 515
ApoI RAATTY 3 cut(s) 273, 576, 640
AseI ATTAAT 2 cut(s) 168, 774
AspS9I GGNCC 1 cut(s) 234
AxyI CCTNAGG 1 cut(s) 238
BalI TGGCCA 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 752
BbvI GCAGC 7 cut(s) 319, 427, 433, 455, 472, 499, 502
BccI CCATC 2 cut(s) 185, 711
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 391, 620
BfaI CTAG 2 cut(s) 137, 533
BfmI CTRYAG 1 cut(s) 441
BglII AGATCT 1 cut(s) 67
BisI GCNGC 8 cut(s) 308, 441, 444, 447, 474, 486, 513, 516
BlsI GCNGC 8 cut(s) 309, 442, 445, 448, 475, 487, 514, 517
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 752
BpmI CTGGAG 1 cut(s) 94
BsaWI WCCGGW 1 cut(s) 617
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
Bse1I ACTGG 2 cut(s) 129, 507
Bse21I CCTNAGG 1 cut(s) 238
Bse3DI GCAATG 1 cut(s) 507
BseAI TCCGGA 1 cut(s) 617
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 4 cut(s) 252, 441, 573, 615
BseNI ACTGG 2 cut(s) 129, 507
BseXI GCAGC 7 cut(s) 319, 427, 433, 455, 472, 499, 502
BsgI GTGCAG 1 cut(s) 445
BshFI GGCC 3 cut(s) 236, 473, 506
BsiSI CCGG 2 cut(s) 537, 618
BslFI GGGAC 1 cut(s) 695
BsmAI GTCTC 2 cut(s) 391, 620
BsmBI CGTCTC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 695
BsnI GGCC 3 cut(s) 236, 473, 506
Bsp13I TCCGGA 1 cut(s) 617
Bsp143I GATC 4 cut(s) 36, 67, 77, 253
BspACI CCGC 1 cut(s) 474
BspANI GGCC 3 cut(s) 236, 473, 506
BspCNI CTCAG 4 cut(s) 251, 442, 574, 616
BspEI TCCGGA 1 cut(s) 617
BspLI GGNNCC 1 cut(s) 235
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 2 cut(s) 129, 507
BssMI GATC 4 cut(s) 36, 67, 77, 253
Bst4CI ACNGT 3 cut(s) 415, 466, 553
Bst6I CTCTTC 1 cut(s) 238
BstC8I GCNNGC 3 cut(s) 94, 460, 826
BstDEI CTNAG 5 cut(s) 238, 450, 582, 624, 840
BstKTI GATC 4 cut(s) 39, 70, 80, 256
BstMAI GTCTC 2 cut(s) 391, 620
BstMBI GATC 4 cut(s) 36, 67, 77, 253
BstMWI GCNNNNNNNGC 4 cut(s) 446, 455, 482, 512
BstSFI CTRYAG 1 cut(s) 441
BstV1I GCAGC 7 cut(s) 319, 427, 433, 455, 472, 499, 502
BstV2I GAAGAC 1 cut(s) 752
BstX2I RGATCY 2 cut(s) 36, 67
BstYI RGATCY 2 cut(s) 36, 67
Bsu36I CCTNAGG 1 cut(s) 238
BsuRI GGCC 3 cut(s) 236, 473, 506
BtsIMutI CAGTG 1 cut(s) 500
Cac8I GCNNGC 3 cut(s) 94, 460, 826
CaiI CAGNNNCTG 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 234
CseI GACGC 4 cut(s) 98, 313, 408, 746
CviAII CATG 1 cut(s) 199
CviJI RGCY 7 cut(s) 236, 364, 446, 473, 485, 506, 536
CviKI_1 RGCY 7 cut(s) 236, 364, 446, 473, 485, 506, 536
DdeI CTNAG 5 cut(s) 238, 450, 582, 624, 840
DpnI GATC 4 cut(s) 38, 69, 79, 255
DpnII GATC 4 cut(s) 36, 67, 77, 253
EaeI YGGCCR 2 cut(s) 471, 504
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
Eco32I GATATC 1 cut(s) 736
Eco57I CTGAAG 1 cut(s) 785
Eco81I CCTNAGG 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 234
EcoRV GATATC 1 cut(s) 736
Esp3I CGTCTC 1 cut(s) 391
FaeI CATG 1 cut(s) 202
FaqI GGGAC 1 cut(s) 695
FatI CATG 1 cut(s) 198
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 8 cut(s) 308, 441, 444, 447, 474, 486, 513, 516
Fsp4HI GCNGC 8 cut(s) 308, 441, 444, 447, 474, 486, 513, 516
FspBI CTAG 2 cut(s) 137, 533
GluI GCNGC 8 cut(s) 308, 441, 444, 447, 474, 486, 513, 516
GsuI CTGGAG 1 cut(s) 94
HaeIII GGCC 3 cut(s) 236, 473, 506
HapII CCGG 2 cut(s) 537, 618
HgaI GACGC 4 cut(s) 98, 313, 408, 746
Hin1II CATG 1 cut(s) 202
HinfI GANTC 6 cut(s) 140, 160, 569, 593, 601, 689
HpaII CCGG 2 cut(s) 537, 618
Hpy188I TCNGA 5 cut(s) 574, 592, 657, 765, 811
Hpy188III TCNNGA 4 cut(s) 73, 240, 581, 618
Hpy99I CGWCG 2 cut(s) 179, 307
HpyAV CCTTC 3 cut(s) 4, 760, 778
HpyCH4III ACNGT 3 cut(s) 415, 466, 553
HpyCH4IV ACGT 4 cut(s) 48, 58, 225, 659
HpyCH4V TGCA 8 cut(s) 54, 96, 266, 443, 462, 500, 613, 646
HpyF10VI GCNNNNNNNGC 4 cut(s) 446, 455, 482, 512
HpyF3I CTNAG 5 cut(s) 238, 450, 582, 624, 840
HpySE526I ACGT 4 cut(s) 48, 58, 225, 659
Hsp92II CATG 1 cut(s) 202
Kpn2I TCCGGA 1 cut(s) 617
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 4 cut(s) 36, 67, 77, 253
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 7 cut(s) 319, 427, 433, 455, 472, 499, 502
MaeI CTAG 2 cut(s) 137, 533
MaeII ACGT 4 cut(s) 48, 58, 225, 659
MaeIII GTNAC 2 cut(s) 194, 415
MalI GATC 4 cut(s) 38, 69, 79, 255
MboI GATC 4 cut(s) 36, 67, 77, 253
MboII GAAGA 7 cut(s) 255, 326, 338, 578, 737, 743, 752
MflI RGATCY 2 cut(s) 36, 67
MlsI TGGCCA 1 cut(s) 506
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 2 cut(s) 149, 587
MnlI CCTC 9 cut(s) 50, 55, 79, 195, 247, 375, 402, 715, 814
Mox20I TGGCCA 1 cut(s) 506
MroI TCCGGA 1 cut(s) 617
MscI TGGCCA 1 cut(s) 506
MseI TTAA 4 cut(s) 26, 168, 608, 774
MslI CAYNNNNRTG 1 cut(s) 467
Msp20I TGGCCA 1 cut(s) 506
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 2 cut(s) 537, 618
MwoI GCNNNNNNNGC 4 cut(s) 446, 455, 482, 512
NdeII GATC 4 cut(s) 36, 67, 77, 253
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 415
OliI CACNNNNGTG 1 cut(s) 467
PfeI GAWTC 4 cut(s) 160, 569, 601, 689
PflFI GACNNNGTC 1 cut(s) 553
PkrI GCNGC 8 cut(s) 309, 442, 445, 448, 475, 487, 514, 517
PleI GAGTC 2 cut(s) 148, 587
PpsI GAGTC 2 cut(s) 148, 587
PshBI ATTAAT 2 cut(s) 168, 774
Psp1406I AACGTT 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 234
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 2 cut(s) 36, 67
PsyI GACNNNGTC 1 cut(s) 553
PvuII CAGCTG 1 cut(s) 446
RseI CAYNNNNRTG 1 cut(s) 467
SaqAI TTAA 4 cut(s) 26, 168, 608, 774
SatI GCNGC 8 cut(s) 308, 441, 444, 447, 474, 486, 513, 516
Sau3AI GATC 4 cut(s) 36, 67, 77, 253
Sau96I GGNCC 1 cut(s) 234
SchI GAGTC 2 cut(s) 149, 587
SetI ASST 7 cut(s) 51, 61, 228, 291, 448, 662, 837
SfcI CTRYAG 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 467
SsiI CCGC 1 cut(s) 474
SspMI CTAG 2 cut(s) 137, 533
TaaI ACNGT 3 cut(s) 415, 466, 553
TaiI ACGT 4 cut(s) 51, 61, 228, 662
TaqI TCGA 1 cut(s) 31
TaqII GACCGA 1 cut(s) 366
TauI GCSGC 1 cut(s) 476
TfiI GAWTC 4 cut(s) 160, 569, 601, 689
Tru1I TTAA 4 cut(s) 26, 168, 608, 774
Tru9I TTAA 4 cut(s) 26, 168, 608, 774
TscAI CASTG 1 cut(s) 507
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 7 cut(s) 307, 440, 443, 446, 485, 512, 515
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 4 cut(s) 23, 117, 421, 744
TspRI CASTG 1 cut(s) 507
Tth111I GACNNNGTC 1 cut(s) 553
VspI ATTAAT 2 cut(s) 168, 774
XapI RAATTY 3 cut(s) 273, 576, 640
XspI CTAG 2 cut(s) 137, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.