RchiOBHm_Chr1g0362831

Thioredoxin-like 3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004408
Physical Location & Seq
Forward (+)
54440010 .. 54442665
2656 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58761

Sequence Viewer

Length: 579 bp
ATGTCCGTAGTTCCGACGTATCCTACTCTCAGGTACCTGAGAACCAGGACCGCACGTGACTCCCCAACCTTCAGTTCTTCGCTCAACAAAGTTTCAATCTTGGGATTCAGGGCGCCAAATTCGCTGGTGCTGTCCCGTAAATTCGATGAAATCCGGGGGCCGATGATAAGGGCTTTGAGCCCAGAAGGGTCGTTGCGAGAGCTCGATGATTCGCCGGTCTCCGTGGAGCACGCGCCCATTTGCAGCGAGGCCCAGTTCGATCGGGTCGTCGCCGAGGCCCAACAGCTCCAGCAATCGTTCGTCGTCGTTTGGATGGCAAGCTGGTGCAGGAAGTGCATATATTTGAAACCAAAGTTGGAGAAGTTGGCAGCCGAGTACTATCCAAGATTGCTGTTCTACAGTGTTGATGTCAATTCAGTTCCACACAAGCTTGTTGCTCGCGCTGAAGTCACTAAAATGCCTACTATACAGCTCTGGAAAGATGGCAAGAAATCAGCTGAGGTAATTGGTGGTCATGGCAAAGCATATTTAGTGATTAATGAAGTTCGTGCAATGATTGAAACAGACGATAGCACATAA

Protein Analysis

192

Amino Acids

21.65

Weight (kDa)

9.13

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 82 - 177 3.5e-16 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 33
AccB1I GGYRCC 2 cut(s) 33, 112
AccII CGCG 2 cut(s) 233, 441
AciI CCGC 1 cut(s) 51
AcsI RAATTY 2 cut(s) 118, 140
AcuI CTGAAG 2 cut(s) 55, 465
AcvI CACGTG 1 cut(s) 56
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 2 cut(s) 35, 377
AgsI TTSAA 3 cut(s) 96, 346, 560
AjnI CCWGG 1 cut(s) 44
AjuI GAANNNNNNNTTGG 4 cut(s) 58, 90, 338, 370
AluBI AGCT 6 cut(s) 202, 286, 321, 430, 472, 497
AluI AGCT 6 cut(s) 202, 286, 321, 430, 472, 497
Alw21I GWGCWC 2 cut(s) 204, 231
Alw26I GTCTC 1 cut(s) 223
AoxI GGCC 3 cut(s) 158, 249, 276
ApeKI GCWGC 2 cut(s) 243, 368
ApoI RAATTY 2 cut(s) 118, 140
AseI ATTAAT 1 cut(s) 537
Asp718I GGTACC 1 cut(s) 33
AspLEI GCGC 3 cut(s) 115, 235, 443
AspS9I GGNCC 4 cut(s) 48, 158, 250, 277
AsuC2I CCSGG 1 cut(s) 155
AvaII GGWCC 1 cut(s) 48
BanI GGYRCC 2 cut(s) 33, 112
BanII GRGCYC 2 cut(s) 182, 204
BbrPI CACGTG 1 cut(s) 56
Bbv12I GWGCWC 2 cut(s) 204, 231
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 2 cut(s) 255, 380
BccI CCATC 2 cut(s) 307, 476
BciT130I CCWGG 1 cut(s) 46
BciVI GTATCC 1 cut(s) 30
BcnI CCSGG 1 cut(s) 155
BcoDI GTCTC 1 cut(s) 223
BfmI CTRYAG 1 cut(s) 397
BfoI RGCGCY 1 cut(s) 116
BfuI GTATCC 1 cut(s) 30
BisI GCNGC 2 cut(s) 244, 369
BlsI GCNGC 2 cut(s) 245, 370
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 2 cut(s) 46, 155
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 4 cut(s) 48, 158, 250, 277
BmiI GGNNCC 3 cut(s) 35, 114, 159
BmrFI CCNGG 2 cut(s) 46, 155
BmrI ACTGGG 1 cut(s) 247
BmuI ACTGGG 1 cut(s) 247
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 498
BpuMI CCSGG 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 56
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 3 cut(s) 154, 222, 273
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 1 cut(s) 253
Bse3DI GCAATG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 46
BseDI CCNNGG 3 cut(s) 154, 222, 273
BseGI GGATG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 3 cut(s) 29, 43, 489
BseNI ACTGG 1 cut(s) 253
BseXI GCAGC 2 cut(s) 255, 380
BsgI GTGCAG 1 cut(s) 346
Bsh1236I CGCG 2 cut(s) 233, 441
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 3 cut(s) 160, 251, 278
BshNI GGYRCC 2 cut(s) 33, 112
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 2 cut(s) 204, 231
BsiSI CCGG 2 cut(s) 154, 215
BslFI GGGAC 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 3 cut(s) 160, 251, 278
Bso31I GGTCTC 1 cut(s) 223
Bsp1286I GDGCHC 3 cut(s) 182, 204, 231
Bsp143I GATC 1 cut(s) 259
BspACI CCGC 1 cut(s) 51
BspANI GGCC 3 cut(s) 160, 251, 278
BspCNI CTCAG 3 cut(s) 30, 42, 490
BspFNI CGCG 2 cut(s) 233, 441
BspLI GGNNCC 3 cut(s) 35, 114, 159
BspT107I GGYRCC 2 cut(s) 33, 112
BspTNI GGTCTC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 1 cut(s) 253
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 3 cut(s) 154, 222, 273
BssMI GATC 1 cut(s) 259
BssNI GRCGYC 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 46
Bst4CI ACNGT 1 cut(s) 401
BstACI GRCGYC 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 333
BstBAI YACGTR 1 cut(s) 56
BstC8I GCNNGC 3 cut(s) 231, 319, 439
BstDEI CTNAG 3 cut(s) 29, 38, 498
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 318
BstFNI CGCG 2 cut(s) 233, 441
BstH2I RGCGCY 1 cut(s) 116
BstHHI GCGC 3 cut(s) 115, 235, 443
BstKTI GATC 1 cut(s) 262
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 1 cut(s) 259
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 2 cut(s) 121, 333
BstNI CCWGG 1 cut(s) 46
BstSCI CCNGG 2 cut(s) 44, 153
BstSFI CTRYAG 1 cut(s) 397
BstUI CGCG 2 cut(s) 233, 441
BstV1I GCAGC 2 cut(s) 255, 380
BsuI GTATCC 1 cut(s) 30
BsuRI GGCC 3 cut(s) 160, 251, 278
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 3 cut(s) 231, 319, 439
CfoI GCGC 3 cut(s) 115, 235, 443
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 4 cut(s) 48, 158, 250, 277
Csp6I GTAC 2 cut(s) 34, 376
CviAII CATG 1 cut(s) 515
CviQI GTAC 2 cut(s) 34, 376
DdeI CTNAG 3 cut(s) 29, 38, 498
DinI GGCGCC 1 cut(s) 114
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
Ecl136II GAGCTC 1 cut(s) 202
Eco24I GRGCYC 2 cut(s) 182, 204
Eco31I GGTCTC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 2 cut(s) 55, 465
Eco72I CACGTG 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 44
EcoT38I GRGCYC 2 cut(s) 182, 204
EgeI GGCGCC 1 cut(s) 114
EheI GGCGCC 1 cut(s) 114
FaeI CATG 1 cut(s) 518
FaiI YATR 6 cut(s) 338, 340, 467, 516, 526, 577
FaqI GGGAC 1 cut(s) 118
FatI CATG 1 cut(s) 514
Fnu4HI GCNGC 2 cut(s) 244, 369
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 2 cut(s) 182, 204
Fsp4HI GCNGC 2 cut(s) 244, 369
GlaI GCGC 3 cut(s) 114, 234, 442
GluI GCNGC 2 cut(s) 244, 369
GsuI CTGGAG 1 cut(s) 272
HaeII RGCGCY 1 cut(s) 116
HaeIII GGCC 3 cut(s) 160, 251, 278
HapII CCGG 2 cut(s) 154, 215
HhaI GCGC 3 cut(s) 115, 235, 443
Hin1I GRCGYC 1 cut(s) 113
Hin1II CATG 1 cut(s) 518
Hin6I GCGC 3 cut(s) 113, 233, 441
HinP1I GCGC 3 cut(s) 113, 233, 441
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 3 cut(s) 59, 105, 209
HpaII CCGG 2 cut(s) 154, 215
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 1 cut(s) 475
Hpy99I CGWCG 4 cut(s) 19, 272, 305, 308
HpyAV CCTTC 2 cut(s) 79, 179
HpyCH4III ACNGT 1 cut(s) 401
HpyCH4IV ACGT 2 cut(s) 17, 55
HpyCH4V TGCA 4 cut(s) 243, 327, 336, 551
HpyF10VI GCNNNNNNNGC 2 cut(s) 121, 333
HpyF3I CTNAG 3 cut(s) 29, 38, 498
HpySE526I ACGT 2 cut(s) 17, 55
Hsp92I GRCGYC 1 cut(s) 113
Hsp92II CATG 1 cut(s) 518
HspAI GCGC 3 cut(s) 113, 233, 441
KasI GGCGCC 1 cut(s) 112
KpnI GGTACC 1 cut(s) 37
Kzo9I GATC 1 cut(s) 259
LmnI GCTCC 2 cut(s) 226, 291
Lsp1109I GCAGC 2 cut(s) 255, 380
MaeII ACGT 2 cut(s) 17, 55
MaeIII GTNAC 2 cut(s) 56, 448
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MboII GAAGA 1 cut(s) 69
MhlI GDGCHC 3 cut(s) 182, 204, 231
MluCI AATT 4 cut(s) 118, 140, 412, 504
Mly113I GGCGCC 1 cut(s) 113
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 2 cut(s) 38, 336
MnlI CCTC 3 cut(s) 241, 268, 493
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 2 cut(s) 154, 215
MspR9I CCNGG 2 cut(s) 46, 155
MvaI CCWGG 1 cut(s) 46
MvnI CGCG 2 cut(s) 233, 441
MwoI GCNNNNNNNGC 2 cut(s) 121, 333
NarI GGCGCC 1 cut(s) 113
NciI CCSGG 1 cut(s) 155
NdeII GATC 1 cut(s) 259
NlaIII CATG 1 cut(s) 518
NlaIV GGNNCC 3 cut(s) 35, 114, 159
NmeAIII GCCGAG 2 cut(s) 298, 397
NmuCI GTSAC 2 cut(s) 56, 448
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PfeI GAWTC 2 cut(s) 105, 209
PkrI GCNGC 2 cut(s) 245, 370
Ple19I CGATCG 1 cut(s) 262
PleI GAGTC 1 cut(s) 53
PluTI GGCGCC 1 cut(s) 116
PmaCI CACGTG 1 cut(s) 56
PmlI CACGTG 1 cut(s) 56
PpsI GAGTC 1 cut(s) 53
Ppu21I YACGTR 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 44
PspCI CACGTG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 3 cut(s) 35, 114, 159
PspPI GGNCC 4 cut(s) 48, 158, 250, 277
PvuI CGATCG 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 2 cut(s) 35, 377
RsaNI GTAC 2 cut(s) 34, 376
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 2 cut(s) 244, 369
Sau3AI GATC 1 cut(s) 259
Sau96I GGNCC 4 cut(s) 48, 158, 250, 277
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 2 cut(s) 46, 155
SduI GDGCHC 3 cut(s) 182, 204, 231
SfcI CTRYAG 1 cut(s) 397
SfoI GGCGCC 1 cut(s) 114
SinI GGWCC 1 cut(s) 48
SmiMI CAYNNNNRTG 1 cut(s) 455
Sse9I AATT 4 cut(s) 118, 140, 412, 504
SsiI CCGC 1 cut(s) 51
SspDI GGCGCC 1 cut(s) 112
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 44, 153
TaaI ACNGT 1 cut(s) 401
TaiI ACGT 2 cut(s) 20, 58
TaqI TCGA 3 cut(s) 144, 204, 258
TasI AATT 4 cut(s) 118, 140, 412, 504
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 2 cut(s) 105, 209
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 2 cut(s) 56, 448
TseI GCWGC 2 cut(s) 243, 368
Tsp45I GTSAC 2 cut(s) 56, 448
TspDTI ATGAA 2 cut(s) 162, 555
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 48
VspI ATTAAT 1 cut(s) 537
XapI RAATTY 2 cut(s) 118, 140
ZrmI AGTACT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.