RLG00000027619

Thioredoxin-like 3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004408
Physical Location & Seq
Reverse (-)
13203123 .. 13205250
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027619

Sequence Viewer

Length: 498 bp
ATGTCCGTAGTTCCGACGTATCCTACTCTCAGGTACCTGAGAACCAGGACCGCACGTGACCTGTCACGTGACTCCCCAACCTTTACTTCTTCGCTCAAAGTTTCAATCTTGGGATTCAGGGCGCCAAGTTCGCTGGTCATTTCCAGTAAATTCGATGGAATACGGGGGCCGATGATAAGGGCTTTGAGCCCAGAAGGGTCGTTGCGAGAGCTCGATGATTCGCCGGTCTCGGTGGAGCTCGCGCCCATTTGCAGCGAGGCCCAGTTCGATCGGGTCATCGCCGAGGCCCAACAGCTCCAGCAATCGTTCGTCGTCGTTTGTGTTGATGTCAATTCAGTTCCACACAAGCTTGTTGCTCGCGCTGAAGTCACTAAAATGCCTACTATACAGCTCTGGAAAGATGGCAAGAAACAAGCTGAGGTAATCGGTGGTCATGGCAAAGCATATTTAGTGATTAATGAAGTTCGTGCAATGATTGAAACAGACGATAGCACATAA

Protein Analysis

166

Amino Acids

18.13

Weight (kDa)

7.78

Isoelectric Point (pI)

57.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 96 - 150 5.7e-06 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 33
AccB1I GGYRCC 2 cut(s) 33, 121
AccII CGCG 2 cut(s) 242, 360
AciI CCGC 1 cut(s) 51
AcsI RAATTY 1 cut(s) 149
AcuI CTGAAG 1 cut(s) 384
AcvI CACGTG 2 cut(s) 56, 68
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 1 cut(s) 35
AgsI TTSAA 2 cut(s) 105, 479
AjnI CCWGG 1 cut(s) 44
AjuI GAANNNNNNNTTGG 2 cut(s) 70, 102
AluBI AGCT 6 cut(s) 211, 238, 295, 349, 391, 416
AluI AGCT 6 cut(s) 211, 238, 295, 349, 391, 416
Alw21I GWGCWC 2 cut(s) 213, 240
Alw26I GTCTC 1 cut(s) 232
AoxI GGCC 3 cut(s) 167, 258, 285
ApeKI GCWGC 1 cut(s) 252
ApoI RAATTY 1 cut(s) 149
AseI ATTAAT 1 cut(s) 456
Asp718I GGTACC 1 cut(s) 33
AspLEI GCGC 3 cut(s) 124, 244, 362
AspS9I GGNCC 4 cut(s) 48, 167, 259, 286
AvaII GGWCC 1 cut(s) 48
BanI GGYRCC 2 cut(s) 33, 121
BanII GRGCYC 3 cut(s) 191, 213, 240
BbrPI CACGTG 2 cut(s) 56, 68
Bbv12I GWGCWC 2 cut(s) 213, 240
BbvCI CCTCAGC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 264
BccI CCATC 2 cut(s) 149, 395
BciT130I CCWGG 1 cut(s) 46
BciVI GTATCC 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 232
BfoI RGCGCY 1 cut(s) 125
BfuI GTATCC 1 cut(s) 30
BisI GCNGC 1 cut(s) 253
BlsI GCNGC 1 cut(s) 254
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 4 cut(s) 48, 167, 259, 286
BmiI GGNNCC 3 cut(s) 35, 123, 168
BmrFI CCNGG 1 cut(s) 46
BmrI ACTGGG 1 cut(s) 256
BmuI ACTGGG 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 417
BsaAI YACGTR 2 cut(s) 56, 68
BsaHI GRCGYC 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 1 cut(s) 282
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 2 cut(s) 144, 262
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 46
BseDI CCNNGG 1 cut(s) 282
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 3 cut(s) 29, 43, 408
BseNI ACTGG 2 cut(s) 144, 262
BseXI GCAGC 1 cut(s) 264
Bsh1236I CGCG 2 cut(s) 242, 360
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 3 cut(s) 169, 260, 287
BshNI GGYRCC 2 cut(s) 33, 121
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 2 cut(s) 213, 240
BsiSI CCGG 1 cut(s) 224
BsmAI GTCTC 1 cut(s) 232
BsnI GGCC 3 cut(s) 169, 260, 287
Bso31I GGTCTC 1 cut(s) 232
Bsp1286I GDGCHC 3 cut(s) 191, 213, 240
Bsp143I GATC 1 cut(s) 268
BspACI CCGC 1 cut(s) 51
BspANI GGCC 3 cut(s) 169, 260, 287
BspCNI CTCAG 3 cut(s) 30, 42, 409
BspFNI CGCG 2 cut(s) 242, 360
BspLI GGNNCC 3 cut(s) 35, 123, 168
BspT107I GGYRCC 2 cut(s) 33, 121
BspTNI GGTCTC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 477
BsrFI RCCGGY 1 cut(s) 223
BsrI ACTGG 2 cut(s) 144, 262
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 1 cut(s) 268
BssNI GRCGYC 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 46
BstACI GRCGYC 1 cut(s) 122
BstBAI YACGTR 2 cut(s) 56, 68
BstC8I GCNNGC 2 cut(s) 240, 358
BstDEI CTNAG 3 cut(s) 29, 38, 417
BstFNI CGCG 2 cut(s) 242, 360
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 3 cut(s) 124, 244, 362
BstKTI GATC 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 1 cut(s) 268
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 44
BstUI CGCG 2 cut(s) 242, 360
BstV1I GCAGC 1 cut(s) 264
BsuI GTATCC 1 cut(s) 30
BsuRI GGCC 3 cut(s) 169, 260, 287
BtgZI GCGATG 1 cut(s) 262
Cac8I GCNNGC 2 cut(s) 240, 358
CfoI GCGC 3 cut(s) 124, 244, 362
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 4 cut(s) 48, 167, 259, 286
Csp6I GTAC 1 cut(s) 34
CviAII CATG 1 cut(s) 434
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 3 cut(s) 29, 38, 417
DinI GGCGCC 1 cut(s) 123
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
Ecl136II GAGCTC 2 cut(s) 211, 238
Eco24I GRGCYC 3 cut(s) 191, 213, 240
Eco31I GGTCTC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 48
Eco53kI GAGCTC 2 cut(s) 211, 238
Eco57I CTGAAG 1 cut(s) 384
Eco72I CACGTG 2 cut(s) 56, 68
EcoICRI GAGCTC 2 cut(s) 211, 238
EcoRII CCWGG 1 cut(s) 44
EcoT38I GRGCYC 3 cut(s) 191, 213, 240
EgeI GGCGCC 1 cut(s) 123
EheI GGCGCC 1 cut(s) 123
FaeI CATG 1 cut(s) 437
FaiI YATR 4 cut(s) 386, 435, 445, 496
FatI CATG 1 cut(s) 433
Fnu4HI GCNGC 1 cut(s) 253
FriOI GRGCYC 3 cut(s) 191, 213, 240
Fsp4HI GCNGC 1 cut(s) 253
GlaI GCGC 3 cut(s) 123, 243, 361
GluI GCNGC 1 cut(s) 253
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 3 cut(s) 169, 260, 287
HapII CCGG 1 cut(s) 224
HhaI GCGC 3 cut(s) 124, 244, 362
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 1 cut(s) 437
Hin6I GCGC 3 cut(s) 122, 242, 360
HinP1I GCGC 3 cut(s) 122, 242, 360
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 3 cut(s) 71, 114, 218
HpaII CCGG 1 cut(s) 224
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 1 cut(s) 394
Hpy99I CGWCG 3 cut(s) 19, 314, 317
HpyAV CCTTC 1 cut(s) 188
HpyCH4IV ACGT 3 cut(s) 17, 55, 67
HpyCH4V TGCA 2 cut(s) 252, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 3 cut(s) 29, 38, 417
HpySE526I ACGT 3 cut(s) 17, 55, 67
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 1 cut(s) 437
HspAI GCGC 3 cut(s) 122, 242, 360
KasI GGCGCC 1 cut(s) 121
KpnI GGTACC 1 cut(s) 37
Kzo9I GATC 1 cut(s) 268
LmnI GCTCC 2 cut(s) 235, 300
Lsp1109I GCAGC 1 cut(s) 264
MaeII ACGT 3 cut(s) 17, 55, 67
MaeIII GTNAC 4 cut(s) 56, 63, 68, 367
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MboII GAAGA 1 cut(s) 81
MhlI GDGCHC 3 cut(s) 191, 213, 240
MluCI AATT 2 cut(s) 149, 331
Mly113I GGCGCC 1 cut(s) 122
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 38
MnlI CCTC 3 cut(s) 250, 277, 412
MseI TTAA 1 cut(s) 456
MslI CAYNNNNRTG 1 cut(s) 374
MspI CCGG 1 cut(s) 224
MspR9I CCNGG 1 cut(s) 46
MvaI CCWGG 1 cut(s) 46
MvnI CGCG 2 cut(s) 242, 360
MwoI GCNNNNNNNGC 1 cut(s) 130
NarI GGCGCC 1 cut(s) 122
NdeII GATC 1 cut(s) 268
NlaIII CATG 1 cut(s) 437
NlaIV GGNNCC 3 cut(s) 35, 123, 168
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 4 cut(s) 56, 63, 68, 367
PfeI GAWTC 2 cut(s) 114, 218
PkrI GCNGC 1 cut(s) 254
Ple19I CGATCG 1 cut(s) 271
PleI GAGTC 1 cut(s) 65
PluTI GGCGCC 1 cut(s) 125
PmaCI CACGTG 2 cut(s) 56, 68
PmlI CACGTG 2 cut(s) 56, 68
PpsI GAGTC 1 cut(s) 65
Ppu21I YACGTR 2 cut(s) 56, 68
PshBI ATTAAT 1 cut(s) 456
Psp124BI GAGCTC 2 cut(s) 213, 240
Psp6I CCWGG 1 cut(s) 44
PspCI CACGTG 2 cut(s) 56, 68
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 3 cut(s) 35, 123, 168
PspPI GGNCC 4 cut(s) 48, 167, 259, 286
PvuI CGATCG 1 cut(s) 271
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 2 cut(s) 213, 240
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 1 cut(s) 253
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 4 cut(s) 48, 167, 259, 286
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 3 cut(s) 191, 213, 240
SfoI GGCGCC 1 cut(s) 123
SinI GGWCC 1 cut(s) 48
SmiMI CAYNNNNRTG 1 cut(s) 374
Sse9I AATT 2 cut(s) 149, 331
SsiI CCGC 1 cut(s) 51
SspDI GGCGCC 1 cut(s) 121
SstI GAGCTC 2 cut(s) 213, 240
StyD4I CCNGG 1 cut(s) 44
TaiI ACGT 3 cut(s) 20, 58, 70
TaqI TCGA 3 cut(s) 153, 213, 267
TasI AATT 2 cut(s) 149, 331
TfiI GAWTC 2 cut(s) 114, 218
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TseFI GTSAC 4 cut(s) 56, 63, 68, 367
TseI GCWGC 1 cut(s) 252
Tsp45I GTSAC 4 cut(s) 56, 63, 68, 367
TspDTI ATGAA 1 cut(s) 474
VpaK11BI GGWCC 1 cut(s) 48
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.