Rh1DG304500

Thioredoxin-like 3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0004408
Physical Location & Seq
Forward (+)
53198590 .. 53201752
3163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG304500.1

Sequence Viewer

Length: 591 bp
ATGTCCGTAGTTCCGACGTATCCTACTCTCAGGTACCTGAGAACCAGGACGGCACGTGACCCGTCACGTGACTCCCCAACCTTCACTTCTTCGCTCAACAAAGTTTCAATCTTGGGATTCAGGGCGCCAAATTCGCTGGTGTTGTCCCGTAAATTCGATGAAATCCGGGGGCCGATGATAAGGGCTTTGAGCCCAGAAGGGTCGCTGCGAGAGCTCGATGATTCGCCGGTCTCCGTGGAGCTCGCGCCCATTTGCAGCGAGGCCCAGTTCGATCGGGTCGTCGCCGAGGCCCAACAGCTCCAGCAATCGTTCGTCGTCGTTTGGATGGCAAGCTGGTGCAGGAAGTGCATATATTTGAAACCAAAGTTGGAGAAGTTGGCAGCCGAGTACTATCCAAGATTGCTGTTCTACAGTGTTGATGTCAATTCAGTTCCACACAAGCTTGTTGCTCGTGCTGAAGTCACTAAAATGCCTACTATACAGCTCTGGAAAGATGGCAAGAAACAAGCTGAGGTAATCGGTGGTCATGGCAAAGCATATTTAGTGATTAATGAAGTTCGTGCAATGATTGAAACAGATGATAGCACATAA

Protein Analysis

196

Amino Acids

22.13

Weight (kDa)

9.13

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 86 - 181 3e-16 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 33
AccB1I GGYRCC 2 cut(s) 33, 124
AccII CGCG 1 cut(s) 245
AcsI RAATTY 2 cut(s) 130, 152
AcuI CTGAAG 1 cut(s) 477
AcvI CACGTG 2 cut(s) 56, 68
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 2 cut(s) 35, 389
AgsI TTSAA 3 cut(s) 108, 358, 572
AjnI CCWGG 1 cut(s) 44
AjuI GAANNNNNNNTTGG 4 cut(s) 70, 102, 350, 382
AluBI AGCT 7 cut(s) 214, 241, 298, 333, 442, 484, 509
AluI AGCT 7 cut(s) 214, 241, 298, 333, 442, 484, 509
Alw21I GWGCWC 2 cut(s) 216, 243
Alw26I GTCTC 1 cut(s) 235
AoxI GGCC 3 cut(s) 170, 261, 288
ApeKI GCWGC 3 cut(s) 205, 255, 380
ApoI RAATTY 2 cut(s) 130, 152
AseI ATTAAT 1 cut(s) 549
Asp718I GGTACC 1 cut(s) 33
AspLEI GCGC 2 cut(s) 127, 247
AspS9I GGNCC 3 cut(s) 170, 262, 289
AsuC2I CCSGG 1 cut(s) 167
BanI GGYRCC 2 cut(s) 33, 124
BanII GRGCYC 3 cut(s) 194, 216, 243
BauI CACGAG 1 cut(s) 450
BbrPI CACGTG 2 cut(s) 56, 68
Bbv12I GWGCWC 2 cut(s) 216, 243
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 3 cut(s) 192, 267, 392
BccI CCATC 2 cut(s) 319, 488
BceAI ACGGC 1 cut(s) 66
BciT130I CCWGG 1 cut(s) 46
BciVI GTATCC 1 cut(s) 30
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 235
BfmI CTRYAG 1 cut(s) 409
BfoI RGCGCY 1 cut(s) 128
BfuI GTATCC 1 cut(s) 30
BisI GCNGC 3 cut(s) 206, 256, 381
BlsI GCNGC 3 cut(s) 207, 257, 382
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 2 cut(s) 46, 167
BmgT120I GGNCC 3 cut(s) 170, 262, 289
BmiI GGNNCC 3 cut(s) 35, 126, 171
BmrFI CCNGG 2 cut(s) 46, 167
BmrI ACTGGG 1 cut(s) 259
BmuI ACTGGG 1 cut(s) 259
BpmI CTGGAG 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 167
BsaAI YACGTR 2 cut(s) 56, 68
BsaHI GRCGYC 1 cut(s) 125
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 3 cut(s) 166, 234, 285
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 1 cut(s) 46
BseDI CCNNGG 3 cut(s) 166, 234, 285
BseGI GGATG 1 cut(s) 330
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 3 cut(s) 29, 43, 501
BseNI ACTGG 1 cut(s) 265
BseXI GCAGC 3 cut(s) 192, 267, 392
BsgI GTGCAG 1 cut(s) 358
Bsh1236I CGCG 1 cut(s) 245
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 3 cut(s) 172, 263, 290
BshNI GGYRCC 2 cut(s) 33, 124
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 216, 243
BsiSI CCGG 2 cut(s) 166, 227
BslFI GGGAC 1 cut(s) 130
BsmAI GTCTC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 130
BsnI GGCC 3 cut(s) 172, 263, 290
Bso31I GGTCTC 1 cut(s) 235
Bsp1286I GDGCHC 3 cut(s) 194, 216, 243
Bsp143I GATC 1 cut(s) 271
BspANI GGCC 3 cut(s) 172, 263, 290
BspCNI CTCAG 3 cut(s) 30, 42, 502
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 3 cut(s) 35, 126, 171
BspT107I GGYRCC 2 cut(s) 33, 124
BspTNI GGTCTC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 570
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 3 cut(s) 166, 234, 285
BssMI GATC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 1 cut(s) 46
Bst4CI ACNGT 1 cut(s) 413
BstACI GRCGYC 1 cut(s) 125
BstAPI GCANNNNNTGC 1 cut(s) 345
BstBAI YACGTR 2 cut(s) 56, 68
BstC8I GCNNGC 2 cut(s) 243, 331
BstDEI CTNAG 3 cut(s) 29, 38, 510
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 1 cut(s) 330
BstFNI CGCG 1 cut(s) 245
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 2 cut(s) 127, 247
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 1 cut(s) 271
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 3 cut(s) 133, 211, 345
BstNI CCWGG 1 cut(s) 46
BstSCI CCNGG 2 cut(s) 44, 165
BstSFI CTRYAG 1 cut(s) 409
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 3 cut(s) 192, 267, 392
BsuI GTATCC 1 cut(s) 30
BsuRI GGCC 3 cut(s) 172, 263, 290
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 418
Cac8I GCNNGC 2 cut(s) 243, 331
CfoI GCGC 2 cut(s) 127, 247
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 3 cut(s) 170, 262, 289
Csp6I GTAC 2 cut(s) 34, 388
CviAII CATG 1 cut(s) 527
CviQI GTAC 2 cut(s) 34, 388
DdeI CTNAG 3 cut(s) 29, 38, 510
DinI GGCGCC 1 cut(s) 126
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
Ecl136II GAGCTC 2 cut(s) 214, 241
Eco24I GRGCYC 3 cut(s) 194, 216, 243
Eco31I GGTCTC 1 cut(s) 235
Eco53kI GAGCTC 2 cut(s) 214, 241
Eco57I CTGAAG 1 cut(s) 477
Eco72I CACGTG 2 cut(s) 56, 68
EcoICRI GAGCTC 2 cut(s) 214, 241
EcoRII CCWGG 1 cut(s) 44
EcoT38I GRGCYC 3 cut(s) 194, 216, 243
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
FaeI CATG 1 cut(s) 530
FaiI YATR 6 cut(s) 350, 352, 479, 528, 538, 589
FaqI GGGAC 1 cut(s) 130
FatI CATG 1 cut(s) 526
Fnu4HI GCNGC 3 cut(s) 206, 256, 381
FokI GGATG 1 cut(s) 337
FriOI GRGCYC 3 cut(s) 194, 216, 243
Fsp4HI GCNGC 3 cut(s) 206, 256, 381
GlaI GCGC 2 cut(s) 126, 246
GluI GCNGC 3 cut(s) 206, 256, 381
GsuI CTGGAG 1 cut(s) 284
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 3 cut(s) 172, 263, 290
HapII CCGG 2 cut(s) 166, 227
HhaI GCGC 2 cut(s) 127, 247
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 1 cut(s) 530
Hin6I GCGC 2 cut(s) 125, 245
HinP1I GCGC 2 cut(s) 125, 245
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 3 cut(s) 71, 117, 221
HpaII CCGG 2 cut(s) 166, 227
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 1 cut(s) 487
Hpy99I CGWCG 4 cut(s) 19, 284, 317, 320
HpyAV CCTTC 2 cut(s) 91, 191
HpyCH4III ACNGT 1 cut(s) 413
HpyCH4IV ACGT 3 cut(s) 17, 55, 67
HpyCH4V TGCA 4 cut(s) 255, 339, 348, 563
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 211, 345
HpyF3I CTNAG 3 cut(s) 29, 38, 510
HpySE526I ACGT 3 cut(s) 17, 55, 67
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 1 cut(s) 530
HspAI GCGC 2 cut(s) 125, 245
KasI GGCGCC 1 cut(s) 124
KpnI GGTACC 1 cut(s) 37
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 2 cut(s) 238, 303
Lsp1109I GCAGC 3 cut(s) 192, 267, 392
MaeII ACGT 3 cut(s) 17, 55, 67
MaeIII GTNAC 4 cut(s) 56, 63, 68, 460
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 1 cut(s) 81
MhlI GDGCHC 3 cut(s) 194, 216, 243
MluCI AATT 3 cut(s) 130, 152, 424
Mly113I GGCGCC 1 cut(s) 125
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 2 cut(s) 38, 348
MnlI CCTC 3 cut(s) 253, 280, 505
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 467
MspI CCGG 2 cut(s) 166, 227
MspR9I CCNGG 2 cut(s) 46, 167
MvaI CCWGG 1 cut(s) 46
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 3 cut(s) 133, 211, 345
NarI GGCGCC 1 cut(s) 125
NciI CCSGG 1 cut(s) 167
NdeII GATC 1 cut(s) 271
NlaIII CATG 1 cut(s) 530
NlaIV GGNNCC 3 cut(s) 35, 126, 171
NmeAIII GCCGAG 2 cut(s) 310, 409
NmuCI GTSAC 4 cut(s) 56, 63, 68, 460
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PfeI GAWTC 2 cut(s) 117, 221
PkrI GCNGC 3 cut(s) 207, 257, 382
Ple19I CGATCG 1 cut(s) 274
PleI GAGTC 1 cut(s) 65
PluTI GGCGCC 1 cut(s) 128
PmaCI CACGTG 2 cut(s) 56, 68
PmlI CACGTG 2 cut(s) 56, 68
PpsI GAGTC 1 cut(s) 65
Ppu21I YACGTR 2 cut(s) 56, 68
PshBI ATTAAT 1 cut(s) 549
Psp124BI GAGCTC 2 cut(s) 216, 243
Psp6I CCWGG 1 cut(s) 44
PspCI CACGTG 2 cut(s) 56, 68
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 3 cut(s) 35, 126, 171
PspPI GGNCC 3 cut(s) 170, 262, 289
PvuI CGATCG 1 cut(s) 274
RsaI GTAC 2 cut(s) 35, 389
RsaNI GTAC 2 cut(s) 34, 388
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 2 cut(s) 216, 243
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 3 cut(s) 206, 256, 381
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 3 cut(s) 170, 262, 289
ScaI AGTACT 1 cut(s) 389
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 2 cut(s) 46, 167
SduI GDGCHC 3 cut(s) 194, 216, 243
SfcI CTRYAG 1 cut(s) 409
SfoI GGCGCC 1 cut(s) 126
SmiMI CAYNNNNRTG 1 cut(s) 467
Sse9I AATT 3 cut(s) 130, 152, 424
SspDI GGCGCC 1 cut(s) 124
SstI GAGCTC 2 cut(s) 216, 243
StyD4I CCNGG 2 cut(s) 44, 165
TaaI ACNGT 1 cut(s) 413
TaiI ACGT 3 cut(s) 20, 58, 70
TaqI TCGA 3 cut(s) 156, 216, 270
TasI AATT 3 cut(s) 130, 152, 424
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 2 cut(s) 117, 221
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 4 cut(s) 56, 63, 68, 460
TseI GCWGC 3 cut(s) 205, 255, 380
Tsp45I GTSAC 4 cut(s) 56, 63, 68, 460
TspDTI ATGAA 2 cut(s) 174, 567
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 1 cut(s) 418
VspI ATTAAT 1 cut(s) 549
XapI RAATTY 2 cut(s) 130, 152
ZrmI AGTACT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.