RchiOBHm_Chr5g0058071

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006036
Physical Location & Seq
Forward (+)
62181867 .. 62183352
1486 bp
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UTR
Exon/CDS
Intron
PRQ33478

Sequence Viewer

Length: 732 bp
ATGAACAGAGCTGGTGAGGTGGAAAAGGGTATAGATTTCTTGTATAGATTGTTATATTCGGGCACTGGGGTCTCTGTGTATTGTTTGACAGCTGTGGTGGAGGGGATGTGTAGGAATGGAGAGATTAGAAGGAGTAGAGAGTTGGTGCAGGAAATGGCTAGTAGAGGAATTAAACCCAATATCATCACATTCAATATAATGGTGGAAGCTTGTGCTAAGAGATGGAACTTTTCGGAGTTGGATTTGATTTTGCCTCTGATGGAAAAGGAAGGAGTTGCATTTGATGTCAAGACATATGAATTCTTGATCGATGGCTTCACGAGCTCCGGGAAGGTAGAAGAAGCCGAAAGATTGGCTGGGGAGATGCATGACAAGGGATGGAAAGTAAAAACTCATGTATACAATTTGATAATAAATAGCTATTGTAGAAAAGGTTTTCTGGAAGGTGCTCTTTCTTTGTTTTCCAAGATGGCTGAGAGAAGTATATTCCAGAATGCTGATACATACTGGTTTCTAATAAATGGTATTTGCAAGGTTGGAGAGATGGGGCTGGCAATGCAATATGTTAATGAGATGCAGAACAAGGGATTTGAATTAGACAATCATGACTGCAACATCTTGGCTGATGGTTTTCGCAGCAAAGGGATGACCAACGAGCTTTTTGAGTTACATGCGTTGATGGAGAAAAAAAGTTCTAAAGCTAATGGCTTTAAACAGAAGAAAAATGGATAG

Protein Analysis

243

Amino Acids

27.6

Weight (kDa)

6.91

Isoelectric Point (pI)

30.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 24 - 74 7.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 25 - 73 5.7e-11 PPR repeat family
PPR_2 PF13041 131 - 178 1.7e-12 PPR repeat family
PPR_1 PF12854 133 - 157 2.1e-06 PPR repeat
PPR PF01535 133 - 161 1e-07 PPR repeat
PPR_2 PF13041 172 - 211 4.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 399
AcsI RAATTY 1 cut(s) 299
AgsI TTSAA 2 cut(s) 193, 593
AluBI AGCT 7 cut(s) 11, 92, 209, 324, 420, 658, 701
AluI AGCT 7 cut(s) 11, 92, 209, 324, 420, 658, 701
Alw21I GWGCWC 2 cut(s) 326, 451
Alw26I GTCTC 1 cut(s) 76
ApeKI GCWGC 1 cut(s) 636
ApoI RAATTY 1 cut(s) 299
AsuC2I CCSGG 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 326
BauI CACGAG 1 cut(s) 319
Bbv12I GWGCWC 2 cut(s) 326, 451
BbvI GCAGC 1 cut(s) 648
BccI CCATC 8 cut(s) 216, 253, 305, 372, 463, 538, 620, 673
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 1 cut(s) 159
BisI GCNGC 1 cut(s) 637
BlsI GCNGC 1 cut(s) 638
Bme1390I CCNGG 1 cut(s) 328
BmrFI CCNGG 1 cut(s) 328
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 2 cut(s) 354, 564
BmuI ACTGGG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 328
Bsa29I ATCGAT 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bse1I ACTGG 2 cut(s) 70, 512
Bse3DI GCAATG 1 cut(s) 561
BseCI ATCGAT 1 cut(s) 309
BseGI GGATG 3 cut(s) 111, 383, 651
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 2 cut(s) 70, 512
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 1 cut(s) 648
BseYI CCCAGC 1 cut(s) 356
BsgI GTGCAG 1 cut(s) 167
BshVI ATCGAT 1 cut(s) 309
BsiHKAI GWGCWC 2 cut(s) 326, 451
BsiSI CCGG 1 cut(s) 327
BsmAI GTCTC 1 cut(s) 76
BsmI GAATGC 1 cut(s) 499
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 3 cut(s) 65, 326, 451
Bsp143I GATC 1 cut(s) 306
BspCNI CTCAG 1 cut(s) 466
BspDI ATCGAT 1 cut(s) 309
BspHI TCATGA 1 cut(s) 604
BspTNI GGTCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 561
BsrI ACTGG 2 cut(s) 70, 512
BssMI GATC 1 cut(s) 306
BssNAI GTATAC 1 cut(s) 400
BssSI CACGAG 1 cut(s) 319
Bst1107I GTATAC 1 cut(s) 400
Bst2BI CACGAG 1 cut(s) 319
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 2 cut(s) 216, 474
BstF5I GGATG 3 cut(s) 111, 383, 651
BstKTI GATC 1 cut(s) 309
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 1 cut(s) 306
BstMWI GCNNNNNNNGC 2 cut(s) 321, 556
BstNSI RCATGY 1 cut(s) 674
BstSCI CCNGG 1 cut(s) 326
BstSLI GKGCMC 1 cut(s) 65
BstV1I GCAGC 1 cut(s) 648
BstZ17I GTATAC 1 cut(s) 400
Bsu15I ATCGAT 1 cut(s) 309
BsuTUI ATCGAT 1 cut(s) 309
BtsCI GGATG 3 cut(s) 111, 383, 651
BtsIMutI CAGTG 1 cut(s) 63
Cac8I GCNNGC 1 cut(s) 552
CciI TCATGA 1 cut(s) 604
ClaI ATCGAT 1 cut(s) 309
CviAII CATG 4 cut(s) 368, 395, 605, 671
DdeI CTNAG 2 cut(s) 216, 474
DpnI GATC 1 cut(s) 308
DpnII GATC 1 cut(s) 306
DraI TTTAAA 1 cut(s) 712
Ecl136II GAGCTC 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 76
Eco53kI GAGCTC 1 cut(s) 324
EcoICRI GAGCTC 1 cut(s) 324
EcoRI GAATTC 1 cut(s) 299
EcoT22I ATGCAT 1 cut(s) 369
EcoT38I GRGCYC 1 cut(s) 326
FaeI CATG 4 cut(s) 371, 398, 608, 674
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FatI CATG 4 cut(s) 367, 394, 604, 670
FauNDI CATATG 1 cut(s) 295
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 1 cut(s) 637
FokI GGATG 3 cut(s) 118, 390, 658
FriOI GRGCYC 1 cut(s) 326
Fsp4HI GCNGC 1 cut(s) 637
FspBI CTAG 1 cut(s) 159
GluI GCNGC 1 cut(s) 637
GsaI CCCAGC 1 cut(s) 360
HapII CCGG 1 cut(s) 327
Hin1II CATG 4 cut(s) 371, 398, 608, 674
HindIII AAGCTT 1 cut(s) 207
HpaII CCGG 1 cut(s) 327
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 2 cut(s) 235, 258
Hpy188III TCNNGA 6 cut(s) 289, 304, 319, 440, 490, 605
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 4 cut(s) 123, 263, 325, 437
HpyCH4V TGCA 7 cut(s) 148, 278, 367, 531, 559, 577, 612
HpyF10VI GCNNNNNNNGC 2 cut(s) 321, 556
HpyF3I CTNAG 2 cut(s) 216, 474
Hsp92II CATG 4 cut(s) 371, 398, 608, 674
Kzo9I GATC 1 cut(s) 306
LmnI GCTCC 1 cut(s) 329
LpnPI CCDG 8 cut(s) 51, 134, 340, 342, 425, 493, 503, 536
Lsp1109I GCAGC 1 cut(s) 648
LweI GCATC 2 cut(s) 354, 564
MaeI CTAG 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 666
MalI GATC 1 cut(s) 308
MboI GATC 1 cut(s) 306
MboII GAAGA 2 cut(s) 350, 730
MhlI GDGCHC 3 cut(s) 65, 326, 451
MluCI AATT 4 cut(s) 168, 299, 403, 593
MmeI TCCRAC 2 cut(s) 219, 517
MnlI CCTC 4 cut(s) 10, 94, 158, 264
Mph1103I ATGCAT 1 cut(s) 369
MseI TTAA 3 cut(s) 171, 567, 711
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 328
Mva1269I GAATGC 1 cut(s) 499
MwoI GCNNNNNNNGC 2 cut(s) 321, 556
NciI CCSGG 1 cut(s) 328
NdeI CATATG 1 cut(s) 295
NdeII GATC 1 cut(s) 306
NlaIII CATG 4 cut(s) 371, 398, 608, 674
NsiI ATGCAT 1 cut(s) 369
NspI RCATGY 1 cut(s) 674
PagI TCATGA 1 cut(s) 604
PctI GAATGC 1 cut(s) 499
PfoI TCCNGGA 1 cut(s) 326
PkrI GCNGC 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 326
PspFI CCCAGC 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 92
SacI GAGCTC 1 cut(s) 326
SaqAI TTAA 3 cut(s) 171, 567, 711
SatI GCNGC 1 cut(s) 637
Sau3AI GATC 1 cut(s) 306
ScrFI CCNGG 1 cut(s) 328
SduI GDGCHC 3 cut(s) 65, 326, 451
SfaNI GCATC 2 cut(s) 354, 564
Sse9I AATT 4 cut(s) 168, 299, 403, 593
SspMI CTAG 1 cut(s) 159
SstI GAGCTC 1 cut(s) 326
StyD4I CCNGG 1 cut(s) 326
TaqI TCGA 1 cut(s) 309
TasI AATT 4 cut(s) 168, 299, 403, 593
Tru1I TTAA 3 cut(s) 171, 567, 711
Tru9I TTAA 3 cut(s) 171, 567, 711
TscAI CASTG 1 cut(s) 70
TseI GCWGC 1 cut(s) 636
TspDTI ATGAA 2 cut(s) 17, 312
TspRI CASTG 1 cut(s) 70
XapI RAATTY 1 cut(s) 299
XceI RCATGY 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 1 cut(s) 159
Zsp2I ATGCAT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.