RLG00000035192

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006036
Physical Location & Seq
Forward (+)
62626448 .. 62627785
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035192

Sequence Viewer

Length: 1134 bp
ATGTCGATCCATAACGTCCTCCGAACCCTGAAAACTGTGAAAGTAAGAACCCAAGTACCCTTTCAAGAATTCTTTGAAATAATCTCTGCAATCCCAAGTCCCTCAGCTGCTCCTAAACCCTTGAATCCCACCACCATTTCCCTCCTCTCCAAGCTCAACCCCAGCTCTCTACACCTCATCCTCTCAGAACCCACACTCAGCGCCTCCAAATGCCTTGTCTTCTTCAACTTCCTCCTCAAAAACCAGTCCTTTATCTCATTCAAAGTTGACCTCCAAGCCCACTTGACTCTCATTTGCAGGCTTATCAGGGCAAGAACGATCACACATGCCGAGCAAGTTTTCAAGTCCATCTCGGTTGATCAAAATTCCAGGGCTGGTGAGGTGGAAAAGGGTATAGATTTCTTGTATAGATTGTTATATTCGGGCACTGGGGTCTCTGTGTATTCTTTGACAGCTGTGGTGGAGGGGATGTGTAGGAATGGAGAGATTAGAAGGAGTAGAGAGTTGGTGCAGGAAATGGCTAGTAGAGGAATTAAACCCAATATCATCACATTCAATATCATGGTGGAAGCTTGTGCTAAGAGATGGAACTTTTCGGAGTTGGATTTGATTTTGCCTTTGATGGAAAAGGAAGGAGTTGCATTTGATGTCAAGACATATGAATTCTTGATCGATGGCTTCACGAGCTCCGGGAAGGTACAAGAAGCCGAAAGATTGGTTGGGGAGATGCATGACAAGGGATTGAAAGTAAAAACTCATTTATACAATTTGATAATAAATAGCTATTGTAGAAAAGGTTTTCTGGAACGTGCTCTTTCTTTATTTTCCAAGATGGCTGAGAGAAGTATATTCCAGAATGCTGATACATACTGGTTTCTAATAAATGGTATTTGCAAGGTTGGAGAGATGGGGCTGGCAATGCAATATGTTAATGAGATGCAGAACAAGGGATTTGAATTAGACAATCTTGACTGCAACATCTTGGCTGATGGTTTTCGCAGCAAAGGGATGACCAACGAGCTTTTTGAGTTACAGGCGTTGATGGAGAAAAAAAGTTCTAAAGCTAATGGGTTTAAACAGAAGAAAAATGGATATGACTTGATGAGGAAGCAGCCAGCTGTACATAATAATTAA

Protein Analysis

378

Amino Acids

42.7

Weight (kDa)

9.16

Isoelectric Point (pI)

34.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 145 - 195 7.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 146 - 194 8.6e-11 PPR repeat family
PPR_3 PF13812 204 - 262 3.5e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 212 - 243 8.7e-06 PPR repeat
PPR_1 PF12854 247 - 278 4.4e-07 PPR repeat
PPR_2 PF13041 252 - 299 3.9e-12 PPR repeat family
PPR PF01535 255 - 282 2.3e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 68, 364, 662
AfaI GTAC 3 cut(s) 57, 699, 1122
AgsI TTSAA 9 cut(s) 65, 77, 124, 226, 262, 343, 556, 745, 956
AjnI CCWGG 1 cut(s) 368
AjuI GAANNNNNNNTTGG 4 cut(s) 45, 77, 702, 734
Alw21I GWGCWC 2 cut(s) 689, 814
Alw26I GTCTC 1 cut(s) 439
ApeKI GCWGC 3 cut(s) 107, 999, 1111
ApoI RAATTY 3 cut(s) 68, 364, 662
ArsI GACNNNNNNTTYG 2 cut(s) 201, 233
AspLEI GCGC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 691
AsuHPI GGTGA 1 cut(s) 389
BaeGI GKGCMC 1 cut(s) 428
BaeI ACNNNNGTAYC 2 cut(s) 39, 72
BanII GRGCYC 1 cut(s) 689
BauI CACGAG 1 cut(s) 682
BbsI GAAGAC 1 cut(s) 211
Bbv12I GWGCWC 2 cut(s) 689, 814
BbvCI CCTCAGC 1 cut(s) 103
BbvI GCAGC 3 cut(s) 94, 1011, 1123
BccI CCATC 8 cut(s) 356, 579, 616, 668, 826, 901, 983, 1036
BciT130I CCWGG 1 cut(s) 370
BclI TGATCA 1 cut(s) 358
BcnI CCSGG 1 cut(s) 691
BcoDI GTCTC 1 cut(s) 439
BfaI CTAG 1 cut(s) 522
BfoI RGCGCY 1 cut(s) 204
BisI GCNGC 3 cut(s) 108, 1000, 1112
BlsI GCNGC 3 cut(s) 109, 1001, 1113
Bme1390I CCNGG 2 cut(s) 370, 691
BmrFI CCNGG 2 cut(s) 370, 691
BmrI ACTGGG 1 cut(s) 438
BmsI GCATC 2 cut(s) 717, 927
BmuI ACTGGG 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 211
Bpu10I CCTNAGC 1 cut(s) 103
BpuMI CCSGG 1 cut(s) 691
Bsa29I ATCGAT 1 cut(s) 672
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bse1I ACTGG 3 cut(s) 244, 433, 875
Bse3DI GCAATG 1 cut(s) 924
BseBI CCWGG 1 cut(s) 370
BseCI ATCGAT 1 cut(s) 672
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 3 cut(s) 177, 474, 1014
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 4 cut(s) 117, 198, 211, 828
BseNI ACTGG 3 cut(s) 244, 433, 875
BseRI GAGGAG 2 cut(s) 134, 224
BseSI GKGCMC 1 cut(s) 428
BseXI GCAGC 3 cut(s) 94, 1011, 1123
BseYI CCCAGC 1 cut(s) 161
BsgI GTGCAG 1 cut(s) 530
BshVI ATCGAT 1 cut(s) 672
BsiHKAI GWGCWC 2 cut(s) 689, 814
BsiSI CCGG 1 cut(s) 690
BslFI GGGAC 1 cut(s) 84
BsmAI GTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 1 cut(s) 862
Bso31I GGTCTC 1 cut(s) 439
Bsp1286I GDGCHC 3 cut(s) 428, 689, 814
Bsp1407I TGTACA 1 cut(s) 1120
Bsp143I GATC 4 cut(s) 6, 318, 358, 669
BspCNI CTCAG 4 cut(s) 116, 197, 210, 829
BspDI ATCGAT 1 cut(s) 672
BspTNI GGTCTC 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 924
BsrGI TGTACA 1 cut(s) 1120
BsrI ACTGG 3 cut(s) 244, 433, 875
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 4 cut(s) 6, 318, 358, 669
BssSI CACGAG 1 cut(s) 682
Bst2BI CACGAG 1 cut(s) 682
Bst2UI CCWGG 1 cut(s) 370
Bst4CI ACNGT 1 cut(s) 37
BstAUI TGTACA 1 cut(s) 1120
BstC8I GCNNGC 3 cut(s) 299, 915, 1116
BstDEI CTNAG 5 cut(s) 103, 184, 197, 579, 837
BstF5I GGATG 3 cut(s) 177, 474, 1014
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 4 cut(s) 9, 321, 361, 672
BstMAI GTCTC 1 cut(s) 439
BstMBI GATC 4 cut(s) 6, 318, 358, 669
BstMWI GCNNNNNNNGC 2 cut(s) 684, 919
BstNI CCWGG 1 cut(s) 370
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 368, 689
BstSLI GKGCMC 1 cut(s) 428
BstV1I GCAGC 3 cut(s) 94, 1011, 1123
BstV2I GAAGAC 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 672
BsuTUI ATCGAT 1 cut(s) 672
BtsCI GGATG 3 cut(s) 177, 474, 1014
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 3 cut(s) 299, 915, 1116
CfoI GCGC 1 cut(s) 203
ClaI ATCGAT 1 cut(s) 672
Csp6I GTAC 3 cut(s) 56, 698, 1121
CviAII CATG 3 cut(s) 326, 562, 731
CviQI GTAC 3 cut(s) 56, 698, 1121
DdeI CTNAG 5 cut(s) 103, 184, 197, 579, 837
DpnI GATC 4 cut(s) 8, 320, 360, 671
DpnII GATC 4 cut(s) 6, 318, 358, 669
DraI TTTAAA 1 cut(s) 1075
Ecl136II GAGCTC 1 cut(s) 687
Eco24I GRGCYC 1 cut(s) 689
Eco31I GGTCTC 1 cut(s) 439
Eco53kI GAGCTC 1 cut(s) 687
EcoICRI GAGCTC 1 cut(s) 687
EcoRI GAATTC 2 cut(s) 68, 662
EcoRII CCWGG 1 cut(s) 368
EcoT22I ATGCAT 1 cut(s) 732
EcoT38I GRGCYC 1 cut(s) 689
FaeI CATG 3 cut(s) 329, 565, 734
FaqI GGGAC 1 cut(s) 84
FatI CATG 3 cut(s) 325, 561, 730
FauNDI CATATG 1 cut(s) 658
FbaI TGATCA 1 cut(s) 358
Fnu4HI GCNGC 3 cut(s) 108, 1000, 1112
FokI GGATG 3 cut(s) 164, 481, 1021
FriOI GRGCYC 1 cut(s) 689
Fsp4HI GCNGC 3 cut(s) 108, 1000, 1112
FspBI CTAG 1 cut(s) 522
GlaI GCGC 1 cut(s) 202
GluI GCNGC 3 cut(s) 108, 1000, 1112
GsaI CCCAGC 1 cut(s) 165
HaeII RGCGCY 1 cut(s) 204
HapII CCGG 1 cut(s) 690
HhaI GCGC 1 cut(s) 203
Hin1II CATG 3 cut(s) 329, 565, 734
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 570
HinfI GANTC 2 cut(s) 124, 286
HpaII CCGG 1 cut(s) 690
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 3 cut(s) 23, 187, 598
Hpy188III TCNNGA 7 cut(s) 65, 652, 667, 682, 803, 853, 968
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 3 cut(s) 486, 626, 688
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 2 cut(s) 15, 808
HpyCH4V TGCA 9 cut(s) 89, 297, 511, 641, 730, 894, 922, 940, 975
HpyF10VI GCNNNNNNNGC 2 cut(s) 684, 919
HpyF3I CTNAG 5 cut(s) 103, 184, 197, 579, 837
HpySE526I ACGT 2 cut(s) 15, 808
Hsp92II CATG 3 cut(s) 329, 565, 734
HspAI GCGC 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 4 cut(s) 6, 318, 358, 669
LmnI GCTCC 2 cut(s) 115, 692
Lsp1109I GCAGC 3 cut(s) 94, 1011, 1123
LweI GCATC 2 cut(s) 717, 927
MaeI CTAG 1 cut(s) 522
MaeII ACGT 2 cut(s) 15, 808
MaeIII GTNAC 1 cut(s) 1029
MalI GATC 4 cut(s) 8, 320, 360, 671
MboI GATC 4 cut(s) 6, 318, 358, 669
MboII GAAGA 3 cut(s) 211, 214, 1093
MhlI GDGCHC 3 cut(s) 428, 689, 814
MluCI AATT 7 cut(s) 68, 364, 531, 662, 766, 956, 1129
MlyI GAGTC 1 cut(s) 280
MmeI TCCRAC 2 cut(s) 582, 880
Mph1103I ATGCAT 1 cut(s) 732
MseI TTAA 4 cut(s) 534, 930, 1074, 1132
MspA1I CMGCKG 3 cut(s) 107, 455, 1118
MspI CCGG 1 cut(s) 690
MspR9I CCNGG 2 cut(s) 370, 691
MssI GTTTAAAC 1 cut(s) 1075
Mva1269I GAATGC 1 cut(s) 862
MvaI CCWGG 1 cut(s) 370
MwoI GCNNNNNNNGC 2 cut(s) 684, 919
NciI CCSGG 1 cut(s) 691
NdeI CATATG 1 cut(s) 658
NdeII GATC 4 cut(s) 6, 318, 358, 669
NlaIII CATG 3 cut(s) 329, 565, 734
NmeAIII GCCGAG 1 cut(s) 355
NsiI ATGCAT 1 cut(s) 732
NspI RCATGY 1 cut(s) 329
PctI GAATGC 1 cut(s) 862
PfeI GAWTC 1 cut(s) 124
PfoI TCCNGGA 1 cut(s) 689
PkrI GCNGC 3 cut(s) 109, 1001, 1113
PleI GAGTC 1 cut(s) 280
PmeI GTTTAAAC 1 cut(s) 1075
PpsI GAGTC 1 cut(s) 280
Psp124BI GAGCTC 1 cut(s) 689
Psp6I CCWGG 1 cut(s) 368
PspFI CCCAGC 1 cut(s) 161
PspGI CCWGG 1 cut(s) 368
PvuII CAGCTG 3 cut(s) 107, 455, 1118
RsaI GTAC 3 cut(s) 57, 699, 1122
RsaNI GTAC 3 cut(s) 56, 698, 1121
SacI GAGCTC 1 cut(s) 689
SaqAI TTAA 4 cut(s) 534, 930, 1074, 1132
SatI GCNGC 3 cut(s) 108, 1000, 1112
Sau3AI GATC 4 cut(s) 6, 318, 358, 669
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 2 cut(s) 370, 691
SduI GDGCHC 3 cut(s) 428, 689, 814
SfaNI GCATC 2 cut(s) 717, 927
Sse9I AATT 7 cut(s) 68, 364, 531, 662, 766, 956, 1129
SspMI CTAG 1 cut(s) 522
SstI GAGCTC 1 cut(s) 689
StyD4I CCNGG 2 cut(s) 368, 689
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 2 cut(s) 18, 811
TaqI TCGA 2 cut(s) 5, 672
TasI AATT 7 cut(s) 68, 364, 531, 662, 766, 956, 1129
TatI WGTACW 1 cut(s) 1120
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 4 cut(s) 534, 930, 1074, 1132
Tru9I TTAA 4 cut(s) 534, 930, 1074, 1132
TscAI CASTG 1 cut(s) 433
TseI GCWGC 3 cut(s) 107, 999, 1111
TspDTI ATGAA 1 cut(s) 675
TspRI CASTG 1 cut(s) 433
XapI RAATTY 3 cut(s) 68, 364, 662
XceI RCATGY 1 cut(s) 329
XspI CTAG 1 cut(s) 522
Zsp2I ATGCAT 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.