RchiOBHm_Chr6g0275781

14 kDa zinc-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003817
Physical Location & Seq
Reverse (-)
37679118 .. 37681894
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24742

Sequence Viewer

Length: 564 bp
ATGGCTGCAGTCACCTCCTCTTCTCTCTTGAGGAACTGTGCAACAACAACTGTGAGAGCTTTTGTGACTGTGAAAGCCTCCAACCCCAATTTCAGAACTTTCCTTCTTCCTCTTCACTCTCGTAGATTTGACAAGATCATAGCAAAGGAAATCCCATCGACCATTGTATACGAGGATGATAAGGTCCTGGCATTTCGGGATATCAACCCACAGGCTCCTGTGCATGTTGTTATCATCCCAAAGAATAGGGATGGCTTAACAGAGCTCGGAAAGGCTGAATCTAGGCATGTGGAAATATTGGGTCAACTTCTATATGCTGGAAAAAAAGTTGCTGAGAAAGAAGGTATTCTCCACGGGTTTCGTGTGGTTATCAACAATGGTCCAGAGGGATGTAAGTCATCATTTTATAATGTTTGTCTGTGCCTTCCTTTCACATCTGTGCAATGCTTAGCTTTGTTGACTTCAATCATCAATGTACTCTCTTCATCCAGGTCAATCTGTTTATCATCTCCACTTGCATCTCCTTGGTCTGATTTTTTTTTCCTTGTGGGTATTAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HIT

Protein Analysis

187

Amino Acids

20.52

Weight (kDa)

9.13

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DcpS_C PF11969 43 - 125 4.2e-15 Scavenger mRNA decapping enzyme C-term binding
HIT PF01230 49 - 133 1.4e-17 HIT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 408
AccI GTMKAC 1 cut(s) 168
AfaI GTAC 1 cut(s) 477
AgsI TTSAA 1 cut(s) 465
AjnI CCWGG 2 cut(s) 186, 488
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AleI CACNNNNGTG 1 cut(s) 437
AluBI AGCT 3 cut(s) 59, 265, 452
AluI AGCT 3 cut(s) 59, 265, 452
Alw21I GWGCWC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 5
AseI ATTAAT 1 cut(s) 555
Asp700I GAANNNNTTC 1 cut(s) 345
AspS9I GGNCC 2 cut(s) 184, 380
AsuHPI GGTGA 1 cut(s) 4
AvaII GGWCC 2 cut(s) 184, 380
BanII GRGCYC 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 267
BccI CCATC 2 cut(s) 163, 245
BciT130I CCWGG 2 cut(s) 188, 490
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 448
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 188, 490
Bme18I GGWCC 2 cut(s) 184, 380
BmgT120I GGNCC 2 cut(s) 184, 380
BmiI GGNNCC 1 cut(s) 216
BmrFI CCNGG 2 cut(s) 188, 490
BmsI GCATC 1 cut(s) 527
Bpu1102I GCTNAGC 1 cut(s) 448
BpuEI CTTGAG 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 352, 524
Bse3DI GCAATG 1 cut(s) 449
BseBI CCWGG 2 cut(s) 188, 490
BseDI CCNNGG 2 cut(s) 352, 524
BseGI GGATG 5 cut(s) 181, 234, 256, 395, 485
BseMI GCAATG 1 cut(s) 449
BseMII CTCAG 1 cut(s) 324
BseRI GAGGAG 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 1 cut(s) 135
Bsp1720I GCTNAGC 1 cut(s) 448
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 216
BspMAI CTGCAG 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 352, 524
BssMI GATC 1 cut(s) 135
BssNAI GTATAC 1 cut(s) 169
BssT1I CCWWGG 1 cut(s) 524
Bst1107I GTATAC 1 cut(s) 169
Bst2UI CCWGG 2 cut(s) 188, 490
Bst4CI ACNGT 3 cut(s) 38, 52, 70
Bst6I CTCTTC 3 cut(s) 25, 117, 487
BstDEI CTNAG 2 cut(s) 333, 448
BstDSI CCRYGG 1 cut(s) 352
BstF5I GGATG 5 cut(s) 181, 234, 256, 395, 485
BstKTI GATC 1 cut(s) 138
BstMBI GATC 1 cut(s) 135
BstNI CCWGG 2 cut(s) 188, 490
BstNSI RCATGY 2 cut(s) 227, 290
BstSCI CCNGG 2 cut(s) 186, 488
BstSFI CTRYAG 1 cut(s) 6
BstZ17I GTATAC 1 cut(s) 169
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 5 cut(s) 181, 234, 256, 395, 485
Cfr13I GGNCC 2 cut(s) 184, 380
Csp6I GTAC 1 cut(s) 476
CviAII CATG 2 cut(s) 224, 287
CviJI RGCY 8 cut(s) 5, 59, 77, 215, 255, 265, 275, 452
CviKI_1 RGCY 8 cut(s) 5, 59, 77, 215, 255, 265, 275, 452
CviQI GTAC 1 cut(s) 476
DdeI CTNAG 2 cut(s) 333, 448
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
Eam1104I CTCTTC 3 cut(s) 25, 117, 487
EarI CTCTTC 3 cut(s) 25, 117, 487
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 524
Eco24I GRGCYC 1 cut(s) 267
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 184, 380
Eco53kI GAGCTC 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 184
EcoRII CCWGG 2 cut(s) 186, 488
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 524
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 524
FaeI CATG 2 cut(s) 227, 290
FaiI YATR 7 cut(s) 140, 169, 225, 288, 313, 315, 408
FatI CATG 2 cut(s) 223, 286
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 5 cut(s) 188, 221, 263, 402, 472
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 282
GluI GCNGC 1 cut(s) 6
Hin1II CATG 2 cut(s) 227, 290
HincII GTYRAC 2 cut(s) 305, 459
HindII GTYRAC 2 cut(s) 305, 459
HinfI GANTC 1 cut(s) 278
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 3 cut(s) 169, 305, 459
Hpy188I TCNGA 3 cut(s) 95, 269, 532
Hpy188III TCNNGA 3 cut(s) 28, 197, 383
Hpy8I GTNNAC 3 cut(s) 169, 305, 459
HpyAV CCTTC 3 cut(s) 113, 335, 434
HpyCH4III ACNGT 3 cut(s) 38, 52, 70
HpyCH4V TGCA 5 cut(s) 8, 41, 223, 442, 518
HpyF3I CTNAG 2 cut(s) 333, 448
Hsp92II CATG 2 cut(s) 227, 290
Kzo9I GATC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 8 cut(s) 173, 197, 200, 231, 303, 396, 475, 502
LweI GCATC 1 cut(s) 527
MaeI CTAG 1 cut(s) 282
MaeIII GTNAC 2 cut(s) 10, 64
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MboII GAAGA 4 cut(s) 12, 98, 104, 474
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 7 cut(s) 24, 25, 28, 88, 120, 166, 379
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 2 cut(s) 257, 555
MslI CAYNNNNRTG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 188, 490
MvaI CCWGG 2 cut(s) 188, 490
NdeII GATC 1 cut(s) 135
NlaIII CATG 2 cut(s) 227, 290
NlaIV GGNNCC 1 cut(s) 216
NmuCI GTSAC 2 cut(s) 10, 64
NspI RCATGY 2 cut(s) 227, 290
OliI CACNNNNGTG 1 cut(s) 437
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 1 cut(s) 278
PkrI GCNGC 1 cut(s) 7
PpuMI RGGWCCY 1 cut(s) 184
PshBI ATTAAT 1 cut(s) 555
PsiI TTATAA 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 186, 488
PspGI CCWGG 2 cut(s) 186, 488
PspN4I GGNNCC 1 cut(s) 216
PspPI GGNCC 2 cut(s) 184, 380
PspPPI RGGWCCY 1 cut(s) 184
PstI CTGCAG 1 cut(s) 10
RsaI GTAC 1 cut(s) 477
RsaNI GTAC 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 437
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 2 cut(s) 257, 555
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 135
Sau96I GGNCC 2 cut(s) 184, 380
ScrFI CCNGG 2 cut(s) 188, 490
SduI GDGCHC 1 cut(s) 267
SetI ASST 8 cut(s) 17, 61, 186, 267, 346, 454, 494, 563
SfaNI GCATC 1 cut(s) 527
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 2 cut(s) 184, 380
SmiMI CAYNNNNRTG 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 1 cut(s) 88
SspI AATATT 1 cut(s) 297
SspMI CTAG 1 cut(s) 282
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 2 cut(s) 186, 488
StyI CCWWGG 1 cut(s) 524
TaaI ACNGT 3 cut(s) 38, 52, 70
TaqI TCGA 1 cut(s) 158
TasI AATT 1 cut(s) 88
TatI WGTACW 1 cut(s) 475
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 2 cut(s) 257, 555
Tru9I TTAA 2 cut(s) 257, 555
TseFI GTSAC 2 cut(s) 10, 64
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 10, 64
TspDTI ATGAA 1 cut(s) 474
VpaK11BI GGWCC 2 cut(s) 184, 380
VspI ATTAAT 1 cut(s) 555
XceI RCATGY 2 cut(s) 227, 290
XmiI GTMKAC 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.