Rmu_sc0005654.1_g000009

14 kDa zinc-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005654.1
OG0003817
Physical Location & Seq
Forward (+)
41694 .. 45942
4249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005654.1_g000009.1.cds

Sequence Viewer

Length: 561 bp
ctgagctggccatcttgggcacctccagatgcttcaactctctcaggctcgattgtaagcccaatcaatctctctctctctctctctcccttcaccgatggctacttctccttgtacccctgcaagctcgaagaggtcaacatcgagtgcgacgagaagggcgaagaggtaaaaggcaaagattgcgggaggatagcggaggagagggaggcggtggtggccgtgctccacggcgcgtcgattatgctccggaactgtgcactccaacctgtgagaacttttgtgactgtgaaagcctccaaccccaatttcaaaactttccttcttcctcttcactctcgcagatttgacaagatcatagcaaaggaaatcccatcgaccattgtatacgaggatgataaggtcctggcatttcgggatatcaacccacaggctcctgtgcatgttgttatcatcccaaagaatagggatgggttaacagagctgggaaaggctgaatctaggcatgtggaaatattgggtatacccaacaacaacttgacgaggtccgcattgaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HIT

Protein Analysis

186

Amino Acids

20.56

Weight (kDa)

6.49

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 19
AccI GTMKAC 2 cut(s) 387, 523
AccII CGCG 1 cut(s) 236
AccIII TCCGGA 1 cut(s) 249
AciI CCGC 4 cut(s) 186, 197, 212, 549
AcoI YGGCCR 2 cut(s) 8, 219
AfaI GTAC 1 cut(s) 116
AgsI TTSAA 3 cut(s) 36, 313, 556
AjnI CCWGG 1 cut(s) 405
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 3 cut(s) 6, 127, 484
AluI AGCT 3 cut(s) 6, 127, 484
Alw21I GWGCWC 2 cut(s) 228, 262
Alw44I GTGCAC 1 cut(s) 258
Aor13HI TCCGGA 1 cut(s) 249
AoxI GGCC 2 cut(s) 8, 219
ApaLI GTGCAC 1 cut(s) 258
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 2 cut(s) 403, 546
AsuHPI GGTGA 1 cut(s) 85
AvaII GGWCC 2 cut(s) 403, 546
BaeGI GKGCMC 2 cut(s) 22, 262
BalI TGGCCA 1 cut(s) 10
BanI GGYRCC 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 228, 262
BccI CCATC 4 cut(s) 19, 92, 382, 464
BceAI ACGGC 2 cut(s) 206, 247
BciT130I CCWGG 1 cut(s) 407
BfaI CTAG 1 cut(s) 501
Bme1390I CCNGG 1 cut(s) 407
Bme18I GGWCC 2 cut(s) 403, 546
BmgT120I GGNCC 2 cut(s) 403, 546
BmiI GGNNCC 2 cut(s) 21, 435
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 1 cut(s) 19
BpmI CTGGAG 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 229
BsaWI WCCGGW 1 cut(s) 249
BseAI TCCGGA 1 cut(s) 249
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 3 cut(s) 400, 453, 475
BseMII CTCAG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 215
BseSI GKGCMC 2 cut(s) 22, 262
BseYI CCCAGC 1 cut(s) 484
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 2 cut(s) 10, 221
BshNI GGYRCC 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 228, 262
BsiSI CCGG 1 cut(s) 250
BsnI GGCC 2 cut(s) 10, 221
Bsp1286I GDGCHC 3 cut(s) 22, 228, 262
Bsp13I TCCGGA 1 cut(s) 249
Bsp143I GATC 1 cut(s) 354
BspACI CCGC 4 cut(s) 186, 197, 212, 549
BspANI GGCC 2 cut(s) 10, 221
BspCNI CTCAG 1 cut(s) 56
BspEI TCCGGA 1 cut(s) 249
BspFNI CGCG 1 cut(s) 236
BspLI GGNNCC 2 cut(s) 21, 435
BspT107I GGYRCC 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 1 cut(s) 354
BssNAI GTATAC 2 cut(s) 388, 524
Bst1107I GTATAC 2 cut(s) 388, 524
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 2 cut(s) 257, 289
Bst6I CTCTTC 3 cut(s) 126, 159, 336
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 8, 125
BstDEI CTNAG 2 cut(s) 2, 43
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 3 cut(s) 400, 453, 475
BstFNI CGCG 1 cut(s) 236
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 1 cut(s) 357
BstMBI GATC 1 cut(s) 354
BstMWI GCNNNNNNNGC 2 cut(s) 183, 218
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 2 cut(s) 446, 509
BstSCI CCNGG 1 cut(s) 405
BstSLI GKGCMC 2 cut(s) 22, 262
BstUI CGCG 1 cut(s) 236
BstZ17I GTATAC 2 cut(s) 388, 524
BsuRI GGCC 2 cut(s) 10, 221
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 3 cut(s) 400, 453, 475
Cac8I GCNNGC 2 cut(s) 8, 125
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 2 cut(s) 403, 546
CseI GACGC 1 cut(s) 225
Csp6I GTAC 1 cut(s) 115
CviAII CATG 2 cut(s) 443, 506
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 2 cut(s) 2, 43
DpnI GATC 1 cut(s) 356
DpnII GATC 1 cut(s) 354
EaeI YGGCCR 2 cut(s) 8, 219
Eam1104I CTCTTC 3 cut(s) 126, 159, 336
EarI CTCTTC 3 cut(s) 126, 159, 336
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 2 cut(s) 403, 546
EcoO109I RGGNCCY 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 405
EcoRV GATATC 1 cut(s) 421
FaeI CATG 2 cut(s) 446, 509
FaiI YATR 6 cut(s) 245, 359, 388, 444, 507, 524
FatI CATG 2 cut(s) 442, 505
FauI CCCGC 1 cut(s) 179
FblI GTMKAC 2 cut(s) 387, 523
FokI GGATG 3 cut(s) 407, 440, 482
FspBI CTAG 1 cut(s) 501
GlaI GCGC 1 cut(s) 235
GsaI CCCAGC 1 cut(s) 488
GsuI CTGGAG 1 cut(s) 9
HaeIII GGCC 2 cut(s) 10, 221
HapII CCGG 1 cut(s) 250
HgaI GACGC 1 cut(s) 225
HhaI GCGC 1 cut(s) 236
Hin1II CATG 2 cut(s) 446, 509
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HincII GTYRAC 2 cut(s) 139, 477
HindII GTYRAC 2 cut(s) 139, 477
HinfI GANTC 1 cut(s) 497
HpaI GTTAAC 1 cut(s) 477
HpaII CCGG 1 cut(s) 250
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 5 cut(s) 139, 260, 388, 477, 524
Hpy188III TCNNGA 3 cut(s) 26, 250, 416
Hpy8I GTNNAC 5 cut(s) 139, 260, 388, 477, 524
Hpy99I CGWCG 2 cut(s) 155, 241
HpyAV CCTTC 3 cut(s) 100, 151, 332
HpyCH4III ACNGT 2 cut(s) 257, 289
HpyCH4V TGCA 3 cut(s) 123, 260, 442
HpyF10VI GCNNNNNNNGC 2 cut(s) 183, 218
HpyF3I CTNAG 2 cut(s) 2, 43
Hsp92II CATG 2 cut(s) 446, 509
HspAI GCGC 1 cut(s) 234
Kpn2I TCCGGA 1 cut(s) 249
KspAI GTTAAC 1 cut(s) 477
Kzo9I GATC 1 cut(s) 354
LmnI GCTCC 3 cut(s) 231, 252, 439
LweI GCATC 1 cut(s) 19
MaeI CTAG 1 cut(s) 501
MaeIII GTNAC 1 cut(s) 283
MalI GATC 1 cut(s) 356
MboI GATC 1 cut(s) 354
MboII GAAGA 4 cut(s) 143, 176, 317, 323
MhlI GDGCHC 3 cut(s) 22, 228, 262
MlsI TGGCCA 1 cut(s) 10
MluCI AATT 1 cut(s) 307
MluNI TGGCCA 1 cut(s) 10
MmeI TCCRAC 2 cut(s) 289, 324
Mox20I TGGCCA 1 cut(s) 10
MroI TCCGGA 1 cut(s) 249
MscI TGGCCA 1 cut(s) 10
MseI TTAA 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 10
MspI CCGG 1 cut(s) 250
MspR9I CCNGG 1 cut(s) 407
MvaI CCWGG 1 cut(s) 407
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 2 cut(s) 183, 218
NdeII GATC 1 cut(s) 354
NlaIII CATG 2 cut(s) 446, 509
NlaIV GGNNCC 2 cut(s) 21, 435
NmuCI GTSAC 1 cut(s) 283
NspI RCATGY 2 cut(s) 446, 509
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 159
PfeI GAWTC 1 cut(s) 497
PflFI GACNNNGTC 1 cut(s) 544
PpuMI RGGWCCY 1 cut(s) 403
Psp5II RGGWCCY 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 405
PspFI CCCAGC 1 cut(s) 484
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 2 cut(s) 21, 435
PspPI GGNCC 2 cut(s) 403, 546
PspPPI RGGWCCY 1 cut(s) 403
PsyI GACNNNGTC 1 cut(s) 544
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SaqAI TTAA 1 cut(s) 476
Sau3AI GATC 1 cut(s) 354
Sau96I GGNCC 2 cut(s) 403, 546
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 3 cut(s) 22, 228, 262
SetI ASST 9 cut(s) 8, 25, 129, 138, 171, 271, 405, 486, 548
SfaNI GCATC 1 cut(s) 19
SinI GGWCC 2 cut(s) 403, 546
Sse9I AATT 1 cut(s) 307
SsiI CCGC 4 cut(s) 186, 197, 212, 549
SspI AATATT 1 cut(s) 516
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 405
TaaI ACNGT 2 cut(s) 257, 289
TaqI TCGA 5 cut(s) 50, 129, 144, 239, 377
TasI AATT 1 cut(s) 307
TfiI GAWTC 1 cut(s) 497
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
Tth111I GACNNNGTC 1 cut(s) 544
VneI GTGCAC 1 cut(s) 258
VpaK11BI GGWCC 2 cut(s) 403, 546
XceI RCATGY 2 cut(s) 446, 509
XmiI GTMKAC 2 cut(s) 387, 523
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.