RchiOBHm_Chr6g0290161

Belongs to the BI1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006198
Physical Location & Seq
Forward (+)
53294740 .. 53296354
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26032

Sequence Viewer

Length: 726 bp
ATGTGGAGCCAACCCTACCGGAAAGATGACTTGGAGTCCGGATCCCGGCCGCTCTACCCCATGATGCTCGAGAGCCCCGAGCTCCGCTGGTCCTTCATCCGCAAAATCTACTCCATCATCGCCGTCCAACTGCTCGTCACCATCGCCGTCGCCGCCACCGTCGTCTCTTACCACCCGATTGCCAACTTCTTCGTCAGTACCGGCGCCGGCTTGGCCCTCTACATAGTCCTCATCATCATTCCCTTCATCGTGTTGTGCCCGCTGTACTATTACCACCAGAGGCATCCTGTGAATTACCTCCTGCTTGGGATTTTCACCATCTCTCTTGCCTTTGTGGTGGGATTGACTTGCGCTTTTACTAGCGGGAAGGTTATTTTGGAGTCTGTGATACTCACCGTTGTGGTTGTAATTGCTCTCACCCTGTACACATTTTGGGCTGCAAGGAGAGGTCATGATTTCAATTTTCTTGGTCCTTTCTTGACCGGAGCGTTGCTCGTTCTCATTGTGTTTTCTCTGATTCAGGTTTTCTTCCCTCTGGGTAAGCTCTCTGTGATGATATACGGGTGCTTGGCATCGATCATCTTTTGTGGATACATTGTGTATGACACAGACAATCTCATCAAGCGCTACTCTTACGACGAGTACATTTGGGCTGCGGTCTCGTTGTATTTGGATATCATCAACCTGTTCCTTGCATTGCTGACTATTTTCAGAAGTGCTGAATAG

Protein Analysis

241

Amino Acids

27.27

Weight (kDa)

7.65

Isoelectric Point (pI)

25.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bax1-I PF01027 31 - 237 2.7e-49 Inhibitor of apoptosis-promoting Bax1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 203
AccBSI CCGCTC 1 cut(s) 52
AccIII TCCGGA 1 cut(s) 38
AciI CCGC 7 cut(s) 50, 85, 100, 153, 260, 363, 656
AclWI GGATC 2 cut(s) 36, 49
AcoI YGGCCR 1 cut(s) 47
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 4 cut(s) 199, 266, 425, 644
AfeI AGCGCT 1 cut(s) 626
AfiI CCNNNNNNNGG 1 cut(s) 45
AgsI TTSAA 1 cut(s) 460
AhdI GACNNNNNGTC 1 cut(s) 34
AjuI GAANNNNNNNTTGG 4 cut(s) 14, 46, 359, 391
AleI CACNNNNGTG 1 cut(s) 398
AluBI AGCT 2 cut(s) 82, 544
AluI AGCT 2 cut(s) 82, 544
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 2 cut(s) 169, 664
AlwI GGATC 2 cut(s) 36, 49
Ama87I CYCGRG 2 cut(s) 68, 77
Aor13HI TCCGGA 1 cut(s) 38
Aor51HI AGCGCT 1 cut(s) 626
AoxI GGCC 2 cut(s) 47, 213
ApeKI GCWGC 2 cut(s) 437, 653
AspLEI GCGC 3 cut(s) 206, 353, 627
AspS9I GGNCC 3 cut(s) 90, 214, 470
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 4 cut(s) 130, 307, 385, 409
AvaI CYCGRG 2 cut(s) 68, 77
AvaII GGWCC 2 cut(s) 90, 470
BaeGI GKGCMC 1 cut(s) 260
BamHI GGATCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 2 cut(s) 77, 84
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 424, 640
BccI CCATC 3 cut(s) 122, 149, 326
BceAI ACGGC 2 cut(s) 107, 131
BciVI GTATCC 1 cut(s) 584
BcnI CCSGG 1 cut(s) 46
BcoDI GTCTC 2 cut(s) 169, 664
BfaI CTAG 1 cut(s) 360
BfoI RGCGCY 2 cut(s) 207, 628
BfuI GTATCC 1 cut(s) 584
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 4 cut(s) 50, 153, 438, 654
BlsI GCNGC 4 cut(s) 51, 154, 439, 655
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 2 cut(s) 90, 470
BmeRI GACNNNNNGTC 1 cut(s) 34
BmeT110I CYCGRG 2 cut(s) 68, 77
BmgT120I GGNCC 3 cut(s) 90, 214, 470
BmiI GGNNCC 3 cut(s) 8, 43, 205
BmrFI CCNGG 1 cut(s) 46
BmsI GCATC 3 cut(s) 54, 292, 581
BplI GAGNNNNNCTC 2 cut(s) 477, 509
BpuMI CCSGG 1 cut(s) 46
Bsa29I ATCGAT 1 cut(s) 575
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 664
BsaWI WCCGGW 3 cut(s) 18, 38, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse118I RCCGGY 2 cut(s) 200, 206
Bse3DI GCAATG 1 cut(s) 695
BseAI TCCGGA 1 cut(s) 38
BseCI ATCGAT 1 cut(s) 575
BseGI GGATG 2 cut(s) 96, 283
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMI GCAATG 1 cut(s) 695
BseSI GKGCMC 1 cut(s) 260
BseX3I CGGCCG 1 cut(s) 47
BseXI GCAGC 2 cut(s) 424, 640
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 2 cut(s) 49, 215
BshNI GGYRCC 1 cut(s) 203
BshVI ATCGAT 1 cut(s) 575
BsiEI CGRYCG 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 84
BsiHKCI CYCGRG 2 cut(s) 68, 77
BsiSI CCGG 6 cut(s) 19, 39, 46, 201, 207, 483
BslI CCNNNNNNNGG 1 cut(s) 45
BsmAI GTCTC 2 cut(s) 169, 664
BsmBI CGTCTC 1 cut(s) 169
BsnI GGCC 2 cut(s) 49, 215
Bso31I GGTCTC 1 cut(s) 664
BsoBI CYCGRG 2 cut(s) 68, 77
Bsp1286I GDGCHC 3 cut(s) 77, 84, 260
Bsp13I TCCGGA 1 cut(s) 38
Bsp1407I TGTACA 1 cut(s) 423
Bsp143I GATC 2 cut(s) 41, 576
BspACI CCGC 7 cut(s) 50, 85, 100, 153, 260, 363, 656
BspANI GGCC 2 cut(s) 49, 215
BspDI ATCGAT 1 cut(s) 575
BspEI TCCGGA 1 cut(s) 38
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 3 cut(s) 8, 43, 205
BspPI GGATC 2 cut(s) 36, 49
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 664
BsrBI CCGCTC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 695
BsrFI RCCGGY 2 cut(s) 200, 206
BsrGI TGTACA 1 cut(s) 423
BssAI RCCGGY 2 cut(s) 200, 206
BssMI GATC 2 cut(s) 41, 576
BssNI GRCGYC 1 cut(s) 204
Bst4CI ACNGT 2 cut(s) 160, 397
BstACI GRCGYC 1 cut(s) 204
BstAUI TGTACA 1 cut(s) 423
BstC8I GCNNGC 2 cut(s) 208, 260
BstF5I GGATG 2 cut(s) 96, 283
BstH2I RGCGCY 2 cut(s) 207, 628
BstHHI GCGC 3 cut(s) 206, 353, 627
BstKTI GATC 2 cut(s) 44, 579
BstMAI GTCTC 2 cut(s) 169, 664
BstMBI GATC 2 cut(s) 41, 576
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 2 cut(s) 152, 212
BstSCI CCNGG 1 cut(s) 44
BstSLI GKGCMC 1 cut(s) 260
BstV1I GCAGC 2 cut(s) 424, 640
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BstZI CGGCCG 1 cut(s) 47
Bsu15I ATCGAT 1 cut(s) 575
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 2 cut(s) 49, 215
BsuTUI ATCGAT 1 cut(s) 575
BtgZI GCGATG 2 cut(s) 103, 127
BtsCI GGATG 2 cut(s) 96, 283
Cac8I GCNNGC 2 cut(s) 208, 260
CciI TCATGA 1 cut(s) 451
CfoI GCGC 3 cut(s) 206, 353, 627
Cfr10I RCCGGY 2 cut(s) 200, 206
Cfr13I GGNCC 3 cut(s) 90, 214, 470
ClaI ATCGAT 1 cut(s) 575
Csp6I GTAC 4 cut(s) 198, 265, 424, 643
CviAII CATG 2 cut(s) 61, 452
CviJI RGCY 9 cut(s) 9, 49, 75, 82, 210, 215, 437, 544, 653
CviKI_1 RGCY 9 cut(s) 9, 49, 75, 82, 210, 215, 437, 544, 653
CviQI GTAC 4 cut(s) 198, 265, 424, 643
DinI GGCGCC 1 cut(s) 205
DpnI GATC 2 cut(s) 43, 578
DpnII GATC 2 cut(s) 41, 576
DriI GACNNNNNGTC 1 cut(s) 34
EaeI YGGCCR 1 cut(s) 47
EagI CGGCCG 1 cut(s) 47
Eam1105I GACNNNNNGTC 1 cut(s) 34
Ecl136II GAGCTC 1 cut(s) 82
EclXI CGGCCG 1 cut(s) 47
Eco24I GRGCYC 2 cut(s) 77, 84
Eco31I GGTCTC 1 cut(s) 664
Eco32I GATATC 1 cut(s) 676
Eco47I GGWCC 2 cut(s) 90, 470
Eco47III AGCGCT 1 cut(s) 626
Eco52I CGGCCG 1 cut(s) 47
Eco53kI GAGCTC 1 cut(s) 82
Eco88I CYCGRG 2 cut(s) 68, 77
EcoICRI GAGCTC 1 cut(s) 82
EcoRV GATATC 1 cut(s) 676
EcoT38I GRGCYC 2 cut(s) 77, 84
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 2 cut(s) 64, 455
FaiI YATR 5 cut(s) 62, 224, 453, 559, 603
FatI CATG 2 cut(s) 60, 451
FauI CCCGC 2 cut(s) 267, 356
Fnu4HI GCNGC 4 cut(s) 50, 153, 438, 654
FokI GGATG 2 cut(s) 83, 270
FriOI GRGCYC 2 cut(s) 77, 84
Fsp4HI GCNGC 4 cut(s) 50, 153, 438, 654
FspBI CTAG 1 cut(s) 360
GlaI GCGC 3 cut(s) 205, 352, 626
GluI GCNGC 4 cut(s) 50, 153, 438, 654
HaeII RGCGCY 2 cut(s) 207, 628
HaeIII GGCC 2 cut(s) 49, 215
HapII CCGG 6 cut(s) 19, 39, 46, 201, 207, 483
HhaI GCGC 3 cut(s) 206, 353, 627
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 2 cut(s) 64, 455
Hin6I GCGC 3 cut(s) 204, 351, 625
HinP1I GCGC 3 cut(s) 204, 351, 625
HinfI GANTC 3 cut(s) 35, 380, 517
HpaII CCGG 6 cut(s) 19, 39, 46, 201, 207, 483
HphI GGTGA 4 cut(s) 130, 307, 385, 409
Hpy166II GTNNAC 1 cut(s) 426
Hpy188I TCNGA 2 cut(s) 516, 713
Hpy188III TCNNGA 4 cut(s) 39, 70, 452, 478
Hpy8I GTNNAC 1 cut(s) 426
Hpy99I CGWCG 3 cut(s) 152, 164, 641
HpyAV CCTTC 3 cut(s) 103, 253, 361
HpyCH4III ACNGT 2 cut(s) 160, 397
HpyCH4V TGCA 2 cut(s) 440, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 212
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 2 cut(s) 64, 455
HspAI GCGC 3 cut(s) 204, 351, 625
KasI GGCGCC 1 cut(s) 203
Kpn2I TCCGGA 1 cut(s) 38
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 2 cut(s) 41, 576
LmnI GCTCC 3 cut(s) 6, 87, 485
Lsp1109I GCAGC 2 cut(s) 424, 640
LweI GCATC 3 cut(s) 54, 292, 581
MaeI CTAG 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 136
MalI GATC 2 cut(s) 43, 578
MbiI CCGCTC 1 cut(s) 52
MboI GATC 2 cut(s) 41, 576
MboII GAAGA 2 cut(s) 181, 520
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 3 cut(s) 77, 84, 260
MluCI AATT 3 cut(s) 292, 408, 460
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 2 cut(s) 44, 389
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 6 cut(s) 227, 239, 273, 308, 440, 543
MroI TCCGGA 1 cut(s) 38
MroNI GCCGGC 1 cut(s) 206
MslI CAYNNNNRTG 1 cut(s) 398
MspA1I CMGCKG 2 cut(s) 87, 262
MspI CCGG 6 cut(s) 19, 39, 46, 201, 207, 483
MspR9I CCNGG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 152, 212
NaeI GCCGGC 1 cut(s) 208
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 1 cut(s) 46
NdeII GATC 2 cut(s) 41, 576
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 2 cut(s) 64, 455
NlaIV GGNNCC 3 cut(s) 8, 43, 205
NmuCI GTSAC 1 cut(s) 136
OliI CACNNNNGTG 1 cut(s) 398
PaeR7I CTCGAG 1 cut(s) 68
PagI TCATGA 1 cut(s) 451
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 156
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 517
PkrI GCNGC 4 cut(s) 51, 154, 439, 655
PleI GAGTC 2 cut(s) 43, 388
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 2 cut(s) 43, 388
Psp124BI GAGCTC 1 cut(s) 84
PspN4I GGNNCC 3 cut(s) 8, 43, 205
PspPI GGNCC 3 cut(s) 90, 214, 470
PsuI RGATCY 1 cut(s) 41
RsaI GTAC 4 cut(s) 199, 266, 425, 644
RsaNI GTAC 4 cut(s) 198, 265, 424, 643
RseI CAYNNNNRTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 84
SatI GCNGC 4 cut(s) 50, 153, 438, 654
Sau3AI GATC 2 cut(s) 41, 576
Sau96I GGNCC 3 cut(s) 90, 214, 470
SchI GAGTC 2 cut(s) 44, 389
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 3 cut(s) 77, 84, 260
SetI ASST 7 cut(s) 84, 300, 372, 451, 525, 546, 687
SfaNI GCATC 3 cut(s) 54, 292, 581
SfoI GGCGCC 1 cut(s) 205
Sfr274I CTCGAG 1 cut(s) 68
SinI GGWCC 2 cut(s) 90, 470
SlaI CTCGAG 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 3 cut(s) 292, 408, 460
SsiI CCGC 7 cut(s) 50, 85, 100, 153, 260, 363, 656
SspDI GGCGCC 1 cut(s) 203
SspMI CTAG 1 cut(s) 360
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 44
TaaI ACNGT 2 cut(s) 160, 397
TaqI TCGA 2 cut(s) 69, 575
TasI AATT 3 cut(s) 292, 408, 460
TatI WGTACW 3 cut(s) 264, 423, 642
TauI GCSGC 2 cut(s) 52, 155
TfiI GAWTC 1 cut(s) 517
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 2 cut(s) 437, 653
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 2 cut(s) 85, 235
VpaK11BI GGWCC 2 cut(s) 90, 470
XhoI CTCGAG 1 cut(s) 68
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.