Rorug06G0229200

Belongs to the BI1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001603
Physical Location & Seq
Reverse (-)
36811025 .. 36818365
7341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0229200.1

Sequence Viewer

Length: 3390 bp
ATGGGAAGCAAGCAGCATGACAGTGTTTTTAAAGACGATGAACCTACTACTGTAGACCATGATAATAGTTCTTTGATCCAGGTCGAGTCTTCAACTGATTCAAATGTGAATAATGAATTTAAAAAGTTGAGTGAGGTTGTCGGTGGCATGAATAATACTCCATACATTTATAGACAAGATGTTGTGAGGAGCAAGAATGGTATGATTGGGGTTGTGACTGAAGTTGCTGGTGATTTGGATTCTGATAGTGGCATGTCTGATGATGAGGATGAGGATGAGGATGAAGAAGATGACGACGATGACGACGACCACAACAGTGAGAATCATGAAAATGACAAAGATGGTGGTGATGACTGTAATACTAATGAAAATGGTGACAAGAACCAGAATGGTGGTAATGATAAGACTGGTCCTCTTCCTGCGGACCAGGTTCGAGTGCTTTGGATAGATGAAACTGAGTCAACCCAGAATATCATTGATTTAACAGTTGTTGATCGAGGATTCTTGCATGGAGATTTTGTTGCTGCTGCTTCTGAGCCAACCGGGCAAGTTGGTGTCGTGGTGGATGTCAATATCTCTGTTGATCTGAAAGCTCCTGATGGGTCCATCATAAAAGATGTGTCATCCAATGACTTAAAACGTGTGAGGGAGTTCACTGTTGGTGATTATGTGGTCCTTGGTACCTGGCTGGGTAGAATTGAGGATGTTTTTGATAATGTGACGGTGATGATTGATGATGGTTCCCTATGTAGAATTTTGAGAGCAGATCCGATGGATCTTAAACCACTTTCCAAAAATATGCTTGAAGATGTGCATTTCCCTTATTACCCAGGCCAGCGTGTGAAGGCAAGGTCATCATCGGTTTTCAAGAATTCTTTGTGGCTATCCGGCTCATGGAAGGCTAATCGTCTGGAAGGTACTGTTACTAAAGTTACAGTGGGATCAGTGTTTATTTATTGGATAGCTTCTGCTGGCTGTGGCCCAGATTCTTCTACTGCCCCTGCTAAAGAGCAGGTCCCCAGGAAGTTGAAACTATTGTCTTGTTTCACACATGCAAATTGGCAGTTGGGCGACTGGTGTCTCTTTCCATCATCAGGGTCGTCATCTTCCACTGCCGTAGATAAGGGTTCAAAATTAGAACTTCATCAATTAGATTCTACTCAAATGGGTAATGTGTGTGATTTGGAAGAGAGTTCACTAGAAGAACCAAATGGAAATAGTGCATCCATGGATACTGACCCGGTGTCTGTATTAGATGGAAACAATGGAAATGCTGGAAGTAATACTTCTATTGAATCTACTTCCAGCGGTAGTTCATTGTCAGTCATAAAGGTGCCTGTTTCTCTTCATCGCAAAAAGCTGCGTAAACCTGTGGTTAAAAGAGACAAGAAGGCACGAAAGGAGGAAAATTTTGAAAGATCTTTTTTGATTGCCAATACCAGGACAACAGTTGATGTGGCATGGCAGGATGGTTCAACAGAACGGAAGCTGGCTTCAACCAATTTAATTCCTCTTGATAGTCCTGGTGATCATGAATTTGTTGCTGAACAATATGTGGTGGAGAAGGCATCTGATGATGGTGACAATGCTTTTGAAGCTAGGCGTGTTGGACTCGTGAAAAGCGTCAATGCCAAGGAGCGAACAGCTTGTGTACAGTGGTTGAAGGCAGTTTCCAGAGCTGAAGATCCTAGAGAGTTTGACAAGGAGGAAGTTGTTAGTGTATATGAGCTTGAGGGTCACCCAGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTGTTGCCAGTTTCTGCACAAACTGCTTCGGTATCGGACTTAATTGAGGAGCCAAAGCAGCAAAATCAACCAAGTGAGCTTACATCAGAAGTTTTTGATGAAGCTTCTTCTGTTGATAAATCTTGTATGGATTTCTCAGATCTTTCTTGGGTTGGGAATATAACTGGTCTTAAGAATGGTGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTACGGTTGGACCTCAAGCAATTTATGTTGTTGGTCGAGCTGATGATGATGACTCAATTGCTGGGAGCGAGGTTAGTGATGCTGCTAGTTGGGAAACAGTTGATGATGAGGAGGTGCATGCCCTATTTCCCCTCGAGAGTATAACAGAGGAAGTGGAGATACAGAGTGCCTTTAACATGAATTCTGAAGCAGAGGTGGGTGGAGAAGATAATTCTGGAAGGAACCCTGCATTGTCTGTTCCCTTGGCTGCACTTCGCTTTGTTACCAGACTGGCAAGTGGAATATTTGCTCGGGGACAAAAGAACTTGGATCCAATTTTAGATACTAAAGGTGAAGGTGATTTTGAGCCTCGGGAAGTTGAAATTTTTCAGGGAGATCATTGTGAAGATTCCAGCTCTCAGAAATCTATAGTTGTTGATAGTAGTGTGGAGACCAATCATAAAAATGAAGAGCATGTTTCCTCGGAGACAACAAAAATATTGGATGCAGCAGAAATCTTGTGCAACTTAAGGGCTGAAGGATTGGATGCTAAAGAATGTAGCAAGGGTGATGTTTGCAGTTTCAAACGCTTTGATATAGTCAAAGATCCTTTGGACCATCATTATCTTGGTGCGGCAGGGCAGAATAGCAGTGGTAAGAAGTGGTTCAAGAAGATTCAGCAAGATTGGGGCATCCTTCAAAATAACCTGCCTGATGGCATCTACGTACGCGTTTATGAAGATCGAATGGATCTCTTGAGGGCAGTTATAGTTGGGGCTTATGGGACACCTTACCAAGATGGCCTCTTCTTTTTCGATTTTCATCTCCCTCCGGAGTACCCTGATGTCCCTCCGTCAGCATATTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATACGAAGAAGGCAAAGTCTGTCTTAGTCTGTTAAATACTTGGACTGGCAGAGGAAATGAAGTTTGGGATCCAAAGTCTTCTAGCATCCTTCAAGTTCTAGTTTCACTCCAGGGATTGGTGCTAAATTCTAAGCCCTATTTCAACGAAGCTGGCTATGATAAGCAAATTGGAACAGCTGAAGGGGAGAAAAATTCGTTGTCCTACAATGAGAATACTTTCTTGCTGAATTGCAAGACGATAATGTCTCTTATGCGGAGGCCTCCCAAGGACTTTGAAGAGCTTGTTCAAGACCATTTCAGGAGGCGTGGTTATTATATCCTCAAGGCTTGCGATGCATATATGAAAGGCTACTTGATAGGTTCGCTCACTAAAGATGCCTCTGTTAGTGATAGCAGTGATGCAAATTCGACCTCTGTTGGTTTCAAGTTGATGTTAGCTAAGATTGTGCCAAAGCTGTTCTTGGCTTTGAGTGAGGTTGGAGCCGATTGTCATGAATTTAAGCATCTGCAACAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bax1-I

Protein Analysis

1129

Amino Acids

124.34

Weight (kDa)

4.56

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3-A_UBE2O PF23048 56 - 163 9.1e-06 UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O PF23046 220 - 351 1.7e-42 UBE2O, tandem tSH3-B domain
SH3-B_UBE2O PF23043 510 - 582 3.6e-20 UBE2O, SH3-B domain
SH3-C_UBE2O PF23044 641 - 681 1.8e-06 UBE2O, ubiquitin binding SH3-C domain
UQ_con PF00179 883 - 1015 8.4e-20 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3112
Acc36I ACCTGC 2 cut(s) 1003, 2688
Acc65I GGTACC 1 cut(s) 680
AccB1I GGYRCC 2 cut(s) 680, 1333
AccB7I CCANNNNNTGG 1 cut(s) 2986
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 2703
AccIII TCCGGA 1 cut(s) 2803
AciI CCGC 5 cut(s) 422, 1308, 2606, 2854, 3124
AcuI CTGAAG 5 cut(s) 240, 1701, 2199, 2529, 3069
AfaI GTAC 5 cut(s) 682, 919, 1653, 2700, 2810
AfiI CCNNNNNNNGG 4 cut(s) 894, 1094, 1440, 2986
AflII CTTAAG 2 cut(s) 1949, 2500
AflIII ACRYGT 2 cut(s) 640, 2701
AhdI GACNNNNNGTC 1 cut(s) 1243
AjnI CCWGG 8 cut(s) 78, 426, 683, 829, 1019, 1439, 1522, 2979
AjuI GAANNNNNNNTTGG 2 cut(s) 2917, 2949
AleI CACNNNNGTG 1 cut(s) 315
Alw26I GTCTC 5 cut(s) 1085, 1377, 2417, 2453, 3120
AlwNI CAGNNNCTG 1 cut(s) 1793
Ama87I CYCGRG 3 cut(s) 2126, 2283, 2343
Aor13HI TCCGGA 1 cut(s) 2803
AoxI GGCC 4 cut(s) 832, 979, 2773, 3128
ApeKI GCWGC 8 cut(s) 13, 524, 527, 1360, 1837, 2075, 2240, 2480
ArsI GACNNNNNNTTYG 2 cut(s) 1574, 1606
Asp700I GAANNNNTTC 3 cut(s) 2358, 2636, 3086
Asp718I GGTACC 1 cut(s) 680
AspS9I GGNCC 8 cut(s) 410, 424, 603, 673, 980, 1015, 2003, 2587
AsuC2I CCSGG 2 cut(s) 544, 1241
AvaI CYCGRG 3 cut(s) 2126, 2283, 2343
AvaII GGWCC 7 cut(s) 410, 424, 603, 673, 1015, 2003, 2587
BamHI GGATCC 2 cut(s) 2302, 2938
BanI GGYRCC 2 cut(s) 680, 1333
BarI GAAGNNNNNNTAC 2 cut(s) 2136, 2168
BauI CACGAG 1 cut(s) 1613
BbsI GAAGAC 2 cut(s) 81, 2940
BbvI GCAGC 8 cut(s) 25, 511, 514, 1347, 1849, 2062, 2227, 2492
BceAI ACGGC 1 cut(s) 1100
BciT130I CCWGG 8 cut(s) 80, 428, 685, 831, 1021, 1441, 1524, 2981
BciVI GTATCC 1 cut(s) 1225
BclI TGATCA 1 cut(s) 1528
BcnI CCSGG 2 cut(s) 544, 1241
BcoDI GTCTC 5 cut(s) 1085, 1377, 2417, 2453, 3120
BfaI CTAG 6 cut(s) 1199, 1599, 1689, 2079, 2952, 2969
BfmI CTRYAG 2 cut(s) 51, 2400
BfrI CTTAAG 2 cut(s) 1949, 2500
BfuAI ACCTGC 2 cut(s) 1003, 2688
BfuI GTATCC 1 cut(s) 1225
BglI GCCNNNNNGGC 1 cut(s) 544
BglII AGATCT 2 cut(s) 1418, 1918
BisI GCNGC 9 cut(s) 14, 525, 528, 1361, 1838, 2076, 2241, 2481, 2607
BlsI GCNGC 9 cut(s) 15, 526, 529, 1362, 1839, 2077, 2242, 2482, 2608
Bme18I GGWCC 7 cut(s) 410, 424, 603, 673, 1015, 2003, 2587
BmeRI GACNNNNNGTC 1 cut(s) 1243
BmeT110I CYCGRG 3 cut(s) 2126, 2283, 2343
BmgT120I GGNCC 8 cut(s) 410, 424, 603, 673, 980, 1015, 2003, 2587
BoxI GACNNNNGTC 1 cut(s) 1077
BpiI GAAGAC 2 cut(s) 81, 2940
BpmI CTGGAG 1 cut(s) 2963
BpuEI CTTGAG 4 cut(s) 1751, 1992, 2749, 3176
BpuMI CCSGG 2 cut(s) 544, 1241
BsaAI YACGTR 1 cut(s) 2698
BsaBI GATNNNNATC 1 cut(s) 2793
BsaI GGTCTC 1 cut(s) 2417
BsaWI WCCGGW 1 cut(s) 2803
BsaXI ACNNNNNCTCC 4 cut(s) 504, 534, 3163, 3193
Bsc4I CCNNNNNNNGG 4 cut(s) 894, 1094, 1440, 2986
Bse1I ACTGG 6 cut(s) 412, 1079, 1787, 1948, 2268, 2920
Bse8I GATNNNNATC 1 cut(s) 2793
BseAI TCCGGA 1 cut(s) 2803
BseBI CCWGG 8 cut(s) 80, 428, 685, 831, 1021, 1441, 1524, 2981
BseJI GATNNNNATC 1 cut(s) 2793
BseLI CCNNNNNNNGG 4 cut(s) 894, 1094, 1440, 2986
BseMII CTCAG 4 cut(s) 447, 525, 1929, 2405
BseNI ACTGG 6 cut(s) 412, 1079, 1787, 1948, 2268, 2920
BseRI GAGGAG 3 cut(s) 202, 1841, 2117
BseXI GCAGC 8 cut(s) 25, 511, 514, 1347, 1849, 2062, 2227, 2492
BseYI CCCAGC 2 cut(s) 688, 2054
BsgI GTGCAG 2 cut(s) 1779, 2226
Bsh1236I CGCG 1 cut(s) 2703
BshFI GGCC 4 cut(s) 834, 981, 2775, 3130
BshNI GGYRCC 2 cut(s) 680, 1333
BsiHKCI CYCGRG 3 cut(s) 2126, 2283, 2343
BsiSI CCGG 4 cut(s) 543, 888, 1241, 2804
BsiWI CGTACG 1 cut(s) 2698
BslFI GGGAC 4 cut(s) 1001, 2301, 2770, 2804
BslI CCNNNNNNNGG 4 cut(s) 894, 1094, 1440, 2986
BsmAI GTCTC 5 cut(s) 1085, 1377, 2417, 2453, 3120
BsmFI GGGAC 4 cut(s) 1001, 2301, 2770, 2804
BsnI GGCC 4 cut(s) 834, 981, 2775, 3130
Bso31I GGTCTC 1 cut(s) 2417
BsoBI CYCGRG 3 cut(s) 2126, 2283, 2343
Bsp13I TCCGGA 1 cut(s) 2803
Bsp1407I TGTACA 1 cut(s) 1651
Bsp19I CCATGG 1 cut(s) 1227
BspACI CCGC 5 cut(s) 422, 1308, 2606, 2854, 3124
BspANI GGCC 4 cut(s) 834, 981, 2775, 3130
BspCNI CTCAG 4 cut(s) 448, 526, 1928, 2404
BspEI TCCGGA 1 cut(s) 2803
BspFNI CGCG 1 cut(s) 2703
BspHI TCATGA 3 cut(s) 325, 1531, 3361
BspMI ACCTGC 2 cut(s) 1003, 2688
BspQI GCTCTTC 2 cut(s) 2436, 3141
BspT107I GGYRCC 2 cut(s) 680, 1333
BspTI CTTAAG 2 cut(s) 1949, 2500
BspTNI GGTCTC 1 cut(s) 2417
BsrGI TGTACA 1 cut(s) 1651
BsrI ACTGG 6 cut(s) 412, 1079, 1787, 1948, 2268, 2920
BssSI CACGAG 1 cut(s) 1613
BssT1I CCWWGG 5 cut(s) 676, 1227, 1632, 2235, 3135
Bst2BI CACGAG 1 cut(s) 1613
Bst2UI CCWGG 8 cut(s) 80, 428, 685, 831, 1021, 1441, 1524, 2981
Bst6I CTCTTC 6 cut(s) 420, 1182, 1350, 2436, 2783, 3141
BstAFI CTTAAG 2 cut(s) 1949, 2500
BstAPI GCANNNNNTGC 1 cut(s) 1802
BstAUI TGTACA 1 cut(s) 1651
BstBAI YACGTR 1 cut(s) 2698
BstC8I GCNNGC 8 cut(s) 11, 836, 973, 1491, 2112, 2852, 3022, 3199
BstDEI CTNAG 7 cut(s) 456, 534, 1915, 2391, 2894, 3000, 3309
BstDSI CCRYGG 1 cut(s) 1227
BstEII GGTNACC 1 cut(s) 1736
BstFNI CGCG 1 cut(s) 2703
BstMAI GTCTC 5 cut(s) 1085, 1377, 2417, 2453, 3120
BstMWI GCNNNNNNNGC 5 cut(s) 544, 1595, 1802, 1837, 3203
BstNI CCWGG 8 cut(s) 80, 428, 685, 831, 1021, 1441, 1524, 2981
BstNSI RCATGY 4 cut(s) 256, 1055, 2114, 2450
BstPAI GACNNNNGTC 1 cut(s) 1077
BstPI GGTNACC 1 cut(s) 1736
BstSFI CTRYAG 2 cut(s) 51, 2400
BstSNI TACGTA 1 cut(s) 2698
BstUI CGCG 1 cut(s) 2703
BstV1I GCAGC 8 cut(s) 25, 511, 514, 1347, 1849, 2062, 2227, 2492
BstV2I GAAGAC 2 cut(s) 81, 2940
BstX2I RGATCY 9 cut(s) 766, 775, 1418, 1684, 1918, 2302, 2578, 2722, 2938
BstXI CCANNNNNNTGG 1 cut(s) 392
BstYI RGATCY 9 cut(s) 766, 775, 1418, 1684, 1918, 2302, 2578, 2722, 2938
BsuI GTATCC 1 cut(s) 1225
BsuRI GGCC 4 cut(s) 834, 981, 2775, 3130
BtgI CCRYGG 1 cut(s) 1227
BtgZI GCGATG 2 cut(s) 1334, 3216
BtsI GCAGTG 3 cut(s) 1110, 2629, 3271
BtsIMutI CAGTG 9 cut(s) 28, 322, 654, 942, 951, 1110, 1661, 2629, 3271
BveI ACCTGC 2 cut(s) 1003, 2688
Cac8I GCNNGC 8 cut(s) 11, 836, 973, 1491, 2112, 2852, 3022, 3199
CaiI CAGNNNCTG 1 cut(s) 1793
CciI TCATGA 3 cut(s) 325, 1531, 3361
Cfr13I GGNCC 8 cut(s) 410, 424, 603, 673, 980, 1015, 2003, 2587
CseI GACGC 1 cut(s) 1612
CsiI ACCWGGT 1 cut(s) 426
Csp6I GTAC 5 cut(s) 681, 918, 1652, 2699, 2809
CspCI CAANNNNNGTGG 2 cut(s) 325, 360
CviQI GTAC 5 cut(s) 681, 918, 1652, 2699, 2809
DdeI CTNAG 7 cut(s) 456, 534, 1915, 2391, 2894, 3000, 3309
DraI TTTAAA 2 cut(s) 31, 121
DrdI GACNNNNNNGTC 1 cut(s) 3112
DriI GACNNNNNGTC 1 cut(s) 1243
DseDI GACNNNNNNGTC 1 cut(s) 3112
Eam1104I CTCTTC 6 cut(s) 420, 1182, 1350, 2436, 2783, 3141
Eam1105I GACNNNNNGTC 1 cut(s) 1243
EarI CTCTTC 6 cut(s) 420, 1182, 1350, 2436, 2783, 3141
EciI GGCGGA 1 cut(s) 2869
Eco105I TACGTA 1 cut(s) 2698
Eco130I CCWWGG 5 cut(s) 676, 1227, 1632, 2235, 3135
Eco147I AGGCCT 1 cut(s) 3130
Eco31I GGTCTC 1 cut(s) 2417
Eco47I GGWCC 7 cut(s) 410, 424, 603, 673, 1015, 2003, 2587
Eco57I CTGAAG 5 cut(s) 240, 1701, 2199, 2529, 3069
Eco88I CYCGRG 3 cut(s) 2126, 2283, 2343
Eco91I GGTNACC 1 cut(s) 1736
EcoO109I RGGNCCY 1 cut(s) 1015
EcoO65I GGTNACC 1 cut(s) 1736
EcoRI GAATTC 2 cut(s) 871, 2173
EcoRII CCWGG 8 cut(s) 78, 426, 683, 829, 1019, 1439, 1522, 2979
EcoT14I CCWWGG 5 cut(s) 676, 1227, 1632, 2235, 3135
EcoT22I ATGCAT 1 cut(s) 3208
ErhI CCWWGG 5 cut(s) 676, 1227, 1632, 2235, 3135
FalI AAGNNNNNCTT 6 cut(s) 1270, 1302, 2877, 2909, 3314, 3346
FaqI GGGAC 4 cut(s) 1001, 2301, 2770, 2804
FbaI TGATCA 1 cut(s) 1528
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 9 cut(s) 14, 525, 528, 1361, 1838, 2076, 2241, 2481, 2607
Fsp4HI GCNGC 9 cut(s) 14, 525, 528, 1361, 1838, 2076, 2241, 2481, 2607
FspBI CTAG 6 cut(s) 1199, 1599, 1689, 2079, 2952, 2969
GluI GCNGC 9 cut(s) 14, 525, 528, 1361, 1838, 2076, 2241, 2481, 2607
GsaI CCCAGC 2 cut(s) 692, 2058
GsuI CTGGAG 1 cut(s) 2963
HaeIII GGCC 4 cut(s) 834, 981, 2775, 3130
HapII CCGG 4 cut(s) 543, 888, 1241, 2804
HgaI GACGC 1 cut(s) 1612
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HindIII AAGCTT 1 cut(s) 1881
HpaII CCGG 4 cut(s) 543, 888, 1241, 2804
Hpy166II GTNNAC 6 cut(s) 55, 462, 654, 1196, 1367, 1652
Hpy8I GTNNAC 6 cut(s) 55, 462, 654, 1196, 1367, 1652
Hpy99I CGWCG 2 cut(s) 299, 308
HpyCH4IV ACGT 2 cut(s) 640, 2697
HpyF10VI GCNNNNNNNGC 5 cut(s) 544, 1595, 1802, 1837, 3203
HpyF3I CTNAG 7 cut(s) 456, 534, 1915, 2391, 2894, 3000, 3309
HpySE526I ACGT 2 cut(s) 640, 2697
Kpn2I TCCGGA 1 cut(s) 2803
KpnI GGTACC 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 1528
LguI GCTCTTC 2 cut(s) 2436, 3141
LmnI GCTCC 6 cut(s) 189, 598, 1636, 1828, 2058, 3350
Lsp1109I GCAGC 8 cut(s) 25, 511, 514, 1347, 1849, 2062, 2227, 2492
MabI ACCWGGT 1 cut(s) 426
MaeI CTAG 6 cut(s) 1199, 1599, 1689, 2079, 2952, 2969
MaeII ACGT 2 cut(s) 640, 2697
MaeIII GTNAC 9 cut(s) 214, 374, 718, 922, 931, 1580, 1736, 1969, 2254
MfeI CAATTG 1 cut(s) 2049
MflI RGATCY 9 cut(s) 766, 775, 1418, 1684, 1918, 2302, 2578, 2722, 2938
MluI ACGCGT 1 cut(s) 2701
MlyI GAGTC 4 cut(s) 95, 467, 1605, 2039
MmeI TCCRAC 3 cut(s) 1588, 1981, 3328
Mph1103I ATGCAT 1 cut(s) 3208
MroI TCCGGA 1 cut(s) 2803
MroXI GAANNNNTTC 3 cut(s) 2358, 2636, 3086
MslI CAYNNNNRTG 6 cut(s) 21, 315, 330, 1331, 2436, 2844
MspA1I CMGCKG 2 cut(s) 1308, 3047
MspCI CTTAAG 2 cut(s) 1949, 2500
MspI CCGG 4 cut(s) 543, 888, 1241, 2804
MunI CAATTG 1 cut(s) 2049
MvaI CCWGG 8 cut(s) 80, 428, 685, 831, 1021, 1441, 1524, 2981
MvnI CGCG 1 cut(s) 2703
MwoI GCNNNNNNNGC 5 cut(s) 544, 1595, 1802, 1837, 3203
NciI CCSGG 2 cut(s) 544, 1241
NcoI CCATGG 1 cut(s) 1227
NmuCI GTSAC 5 cut(s) 214, 374, 718, 1580, 1736
NsiI ATGCAT 1 cut(s) 3208
NspI RCATGY 4 cut(s) 256, 1055, 2114, 2450
OliI CACNNNNGTG 1 cut(s) 315
PaeI GCATGC 1 cut(s) 2114
PaeR7I CTCGAG 1 cut(s) 2126
PagI TCATGA 3 cut(s) 325, 1531, 3361
PceI AGGCCT 1 cut(s) 3130
PciSI GCTCTTC 2 cut(s) 2436, 3141
PcsI WCGNNNNNNNCGW 3 cut(s) 300, 303, 1620
PdmI GAANNNNTTC 3 cut(s) 2358, 2636, 3086
PfeI GAWTC 9 cut(s) 98, 239, 322, 501, 986, 1154, 1295, 2381, 2647
Pfl23II CGTACG 1 cut(s) 2698
PflMI CCANNNNNTGG 1 cut(s) 2986
PkrI GCNGC 9 cut(s) 15, 526, 529, 1362, 1839, 2077, 2242, 2482, 2608
PleI GAGTC 4 cut(s) 94, 466, 1605, 2039
PpsI GAGTC 4 cut(s) 94, 466, 1605, 2039
Ppu21I YACGTR 1 cut(s) 2698
PpuMI RGGWCCY 1 cut(s) 1015
PshAI GACNNNNGTC 1 cut(s) 1077
Psp5II RGGWCCY 1 cut(s) 1015
Psp6I CCWGG 8 cut(s) 78, 426, 683, 829, 1019, 1439, 1522, 2979
PspEI GGTNACC 1 cut(s) 1736
PspFI CCCAGC 2 cut(s) 688, 2054
PspGI CCWGG 8 cut(s) 78, 426, 683, 829, 1019, 1439, 1522, 2979
PspLI CGTACG 1 cut(s) 2698
PspPI GGNCC 8 cut(s) 410, 424, 603, 673, 980, 1015, 2003, 2587
PspPPI RGGWCCY 1 cut(s) 1015
PstNI CAGNNNCTG 1 cut(s) 1793
PsuI RGATCY 9 cut(s) 766, 775, 1418, 1684, 1918, 2302, 2578, 2722, 2938
PvuII CAGCTG 1 cut(s) 3047
RsaI GTAC 5 cut(s) 682, 919, 1653, 2700, 2810
RsaNI GTAC 5 cut(s) 681, 918, 1652, 2699, 2809
RseI CAYNNNNRTG 6 cut(s) 21, 315, 330, 1331, 2436, 2844
SapI GCTCTTC 2 cut(s) 2436, 3141
SatI GCNGC 9 cut(s) 14, 525, 528, 1361, 1838, 2076, 2241, 2481, 2607
Sau96I GGNCC 8 cut(s) 410, 424, 603, 673, 980, 1015, 2003, 2587
SchI GAGTC 4 cut(s) 95, 467, 1605, 2039
SexAI ACCWGGT 1 cut(s) 426
SfcI CTRYAG 2 cut(s) 51, 2400
Sfr274I CTCGAG 1 cut(s) 2126
SinI GGWCC 7 cut(s) 410, 424, 603, 673, 1015, 2003, 2587
SlaI CTCGAG 1 cut(s) 2126
SmiMI CAYNNNNRTG 6 cut(s) 21, 315, 330, 1331, 2436, 2844
SmlI CTYRAG 7 cut(s) 1730, 1949, 2007, 2126, 2500, 2728, 3191
SmoI CTYRAG 7 cut(s) 1730, 1949, 2007, 2126, 2500, 2728, 3191
SnaBI TACGTA 1 cut(s) 2698
SphI GCATGC 1 cut(s) 2114
SseBI AGGCCT 1 cut(s) 3130
SsiI CCGC 5 cut(s) 422, 1308, 2606, 2854, 3124
SspI AATATT 2 cut(s) 2277, 2472
SspMI CTAG 6 cut(s) 1199, 1599, 1689, 2079, 2952, 2969
StuI AGGCCT 1 cut(s) 3130
StyI CCWWGG 5 cut(s) 676, 1227, 1632, 2235, 3135
TaiI ACGT 2 cut(s) 643, 2700
TaqI TCGA 8 cut(s) 84, 433, 496, 2029, 2127, 2716, 2787, 3278
TatI WGTACW 1 cut(s) 1651
TauI GCSGC 1 cut(s) 2609
TfiI GAWTC 9 cut(s) 98, 239, 322, 501, 986, 1154, 1295, 2381, 2647
TscAI CASTG 9 cut(s) 28, 322, 661, 942, 951, 1117, 1661, 2629, 3271
TseFI GTSAC 5 cut(s) 214, 374, 718, 1580, 1736
TseI GCWGC 8 cut(s) 13, 524, 527, 1360, 1837, 2075, 2240, 2480
Tsp45I GTSAC 5 cut(s) 214, 374, 718, 1580, 1736
TspGWI ACGGA 2 cut(s) 1498, 2814
TspRI CASTG 9 cut(s) 28, 322, 661, 942, 951, 1117, 1661, 2629, 3271
Van91I CCANNNNNTGG 1 cut(s) 2986
Vha464I CTTAAG 2 cut(s) 1949, 2500
VpaK11BI GGWCC 7 cut(s) 410, 424, 603, 673, 1015, 2003, 2587
XceI RCATGY 4 cut(s) 256, 1055, 2114, 2450
XcmI CCANNNNNNNNNTGG 1 cut(s) 2597
XhoI CTCGAG 1 cut(s) 2126
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 3 cut(s) 2358, 2636, 3086
XspI CTAG 6 cut(s) 1199, 1599, 1689, 2079, 2952, 2969
Zsp2I ATGCAT 1 cut(s) 3208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.