Rh6DG321200

Belongs to the BI1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0006198
Physical Location & Seq
Forward (+)
52676304 .. 52678796
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG321200.1

Sequence Viewer

Length: 825 bp
ATGTGGAGCCAACCCTACCGGAAAGATGACGTGGAGTCCGGATCCCGGCCGCTCTACCCCATGATGCTCGAGAGCCCCGAGCTCCGCTGGTCCTTCATCCGCAAAATCTACTCCATCATCGCCGTCCAACTGCTCGCCACCATCGCCGTCGCCGCCACCGTCGTCTCCTACCACCCGATTGCCAACTTCTTCGTCAGCACCGGCGCCGGCTTGGCCCTCTACATAGTCCTCATCATCACTCCCTTCATCGGTACCTTCTTAATTCTACCTAGTCGATCCCCCTTTTGGTTTTTTTGTGAGCTTTGTTGTGAAATTAGGGTTTCTGATTTTGGTTTTGGTTTGGACGCAGTGTTGTGCCCGCTGTACTATTACCACCAGAGGCATCCTGTGAATTACCTCCTGCTTGGGATTTTCACCATCTCTCTTGCCTTTGTGGTGGGATTGACTTGCGCTTTTACTAGCGGGAAGGTTATTTTGGAGTCTGTGATACTCACCGTTGTGGTTGTAATTGCTCTCACCCTGTACACATTTTGGGCTGCAAGGAGAGGCCAAGATTTCAACTTTCTTGGTCCCTTCTTGGCCGGAGCATTGCTCGTTCTCATTGTGTTTTCTCTGATTCAGGTTTTCTTCCCCCTGGGTAAGCTCTCAGTGATGATATACGGGTGCTTGGCATCGATCATCTTTTGTGGATACATTGTGTATGACACGGACAATCTCATCAAGCGCTACTCTTACGACGAGTACATTTGGGCTGCGGTCTCGTTGTATTTGGATATCATCAACCTGTTCCTTGCATTGCTGACTATTTTCAGAAGTGCTGAATAG

Protein Analysis

274

Amino Acids

30.94

Weight (kDa)

6.29

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bax1-I PF01027 120 - 270 9.4e-40 Inhibitor of apoptosis-promoting Bax1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 251
AccB1I GGYRCC 2 cut(s) 203, 251
AccBSI CCGCTC 1 cut(s) 52
AccIII TCCGGA 1 cut(s) 38
AciI CCGC 7 cut(s) 50, 85, 100, 153, 359, 462, 755
AclWI GGATC 3 cut(s) 36, 49, 270
AcoI YGGCCR 2 cut(s) 47, 579
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 4 cut(s) 253, 365, 524, 743
AfeI AGCGCT 1 cut(s) 725
AfiI CCNNNNNNNGG 3 cut(s) 45, 248, 285
AgsI TTSAA 1 cut(s) 559
AhdI GACNNNNNGTC 1 cut(s) 34
AjiI CACGTC 1 cut(s) 31
AjnI CCWGG 1 cut(s) 633
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AleI CACNNNNGTG 1 cut(s) 497
AluBI AGCT 3 cut(s) 82, 301, 643
AluI AGCT 3 cut(s) 82, 301, 643
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 2 cut(s) 169, 763
AlwI GGATC 3 cut(s) 36, 49, 270
Ama87I CYCGRG 2 cut(s) 68, 77
Aor13HI TCCGGA 1 cut(s) 38
Aor51HI AGCGCT 1 cut(s) 725
AoxI GGCC 4 cut(s) 47, 213, 547, 579
ApeKI GCWGC 2 cut(s) 536, 752
Asp718I GGTACC 1 cut(s) 251
AspLEI GCGC 3 cut(s) 206, 452, 726
AspS9I GGNCC 3 cut(s) 90, 214, 569
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 3 cut(s) 406, 484, 508
AvaI CYCGRG 2 cut(s) 68, 77
AvaII GGWCC 2 cut(s) 90, 569
BaeGI GKGCMC 1 cut(s) 359
BamHI GGATCC 1 cut(s) 41
BanI GGYRCC 2 cut(s) 203, 251
BanII GRGCYC 2 cut(s) 77, 84
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 523, 739
BccI CCATC 3 cut(s) 122, 149, 425
BceAI ACGGC 2 cut(s) 107, 131
BciT130I CCWGG 1 cut(s) 635
BciVI GTATCC 1 cut(s) 683
BcnI CCSGG 1 cut(s) 46
BcoDI GTCTC 2 cut(s) 169, 763
BfaI CTAG 2 cut(s) 270, 459
BfoI RGCGCY 2 cut(s) 207, 727
BfuI GTATCC 1 cut(s) 683
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 4 cut(s) 50, 153, 537, 753
BlsI GCNGC 4 cut(s) 51, 154, 538, 754
Bme1390I CCNGG 2 cut(s) 46, 635
Bme18I GGWCC 2 cut(s) 90, 569
BmeRI GACNNNNNGTC 1 cut(s) 34
BmeT110I CYCGRG 2 cut(s) 68, 77
BmgBI CACGTC 1 cut(s) 31
BmgT120I GGNCC 3 cut(s) 90, 214, 569
BmiI GGNNCC 5 cut(s) 8, 43, 205, 253, 571
BmrFI CCNGG 2 cut(s) 46, 635
BmsI GCATC 3 cut(s) 54, 391, 680
BplI GAGNNNNNCTC 2 cut(s) 576, 608
BpuMI CCSGG 1 cut(s) 46
Bsa29I ATCGAT 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 763
BsaJI CCNNGG 2 cut(s) 633, 634
BsaWI WCCGGW 2 cut(s) 18, 38
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 248, 285
Bse118I RCCGGY 2 cut(s) 200, 206
Bse3DI GCAATG 2 cut(s) 587, 794
BseAI TCCGGA 1 cut(s) 38
BseBI CCWGG 1 cut(s) 635
BseCI ATCGAT 1 cut(s) 674
BseDI CCNNGG 2 cut(s) 633, 634
BseGI GGATG 2 cut(s) 96, 382
BseLI CCNNNNNNNGG 3 cut(s) 45, 248, 285
BseMI GCAATG 2 cut(s) 587, 794
BseMII CTCAG 1 cut(s) 660
BseSI GKGCMC 1 cut(s) 359
BseX3I CGGCCG 1 cut(s) 47
BseXI GCAGC 2 cut(s) 523, 739
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 4 cut(s) 49, 215, 549, 581
BshNI GGYRCC 2 cut(s) 203, 251
BshVI ATCGAT 1 cut(s) 674
BsiEI CGRYCG 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 84
BsiHKCI CYCGRG 2 cut(s) 68, 77
BsiSI CCGG 6 cut(s) 19, 39, 46, 201, 207, 582
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 3 cut(s) 45, 248, 285
BsmAI GTCTC 2 cut(s) 169, 763
BsmBI CGTCTC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 555
BsnI GGCC 4 cut(s) 49, 215, 549, 581
Bso31I GGTCTC 1 cut(s) 763
BsoBI CYCGRG 2 cut(s) 68, 77
Bsp1286I GDGCHC 3 cut(s) 77, 84, 359
Bsp13I TCCGGA 1 cut(s) 38
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 3 cut(s) 41, 275, 675
BspACI CCGC 7 cut(s) 50, 85, 100, 153, 359, 462, 755
BspANI GGCC 4 cut(s) 49, 215, 549, 581
BspCNI CTCAG 1 cut(s) 659
BspDI ATCGAT 1 cut(s) 674
BspEI TCCGGA 1 cut(s) 38
BspLI GGNNCC 5 cut(s) 8, 43, 205, 253, 571
BspPI GGATC 3 cut(s) 36, 49, 270
BspT107I GGYRCC 2 cut(s) 203, 251
BspTNI GGTCTC 1 cut(s) 763
BsrBI CCGCTC 1 cut(s) 52
BsrDI GCAATG 2 cut(s) 587, 794
BsrFI RCCGGY 2 cut(s) 200, 206
BsrGI TGTACA 1 cut(s) 522
BssAI RCCGGY 2 cut(s) 200, 206
BssECI CCNNGG 2 cut(s) 633, 634
BssMI GATC 3 cut(s) 41, 275, 675
BssNI GRCGYC 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 635
Bst4CI ACNGT 2 cut(s) 160, 496
BstACI GRCGYC 1 cut(s) 204
BstAUI TGTACA 1 cut(s) 522
BstC8I GCNNGC 3 cut(s) 135, 208, 359
BstDEI CTNAG 1 cut(s) 646
BstF5I GGATG 2 cut(s) 96, 382
BstH2I RGCGCY 2 cut(s) 207, 727
BstHHI GCGC 3 cut(s) 206, 452, 726
BstKTI GATC 3 cut(s) 44, 278, 678
BstMAI GTCTC 2 cut(s) 169, 763
BstMBI GATC 3 cut(s) 41, 275, 675
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 212
BstNI CCWGG 1 cut(s) 635
BstSCI CCNGG 2 cut(s) 44, 633
BstSLI GKGCMC 1 cut(s) 359
BstV1I GCAGC 2 cut(s) 523, 739
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BstZI CGGCCG 1 cut(s) 47
Bsu15I ATCGAT 1 cut(s) 674
BsuI GTATCC 1 cut(s) 683
BsuRI GGCC 4 cut(s) 49, 215, 549, 581
BsuTUI ATCGAT 1 cut(s) 674
BtgZI GCGATG 2 cut(s) 103, 127
BtrI CACGTC 1 cut(s) 31
BtsCI GGATG 2 cut(s) 96, 382
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 2 cut(s) 354, 654
Cac8I GCNNGC 3 cut(s) 135, 208, 359
CfoI GCGC 3 cut(s) 206, 452, 726
Cfr10I RCCGGY 2 cut(s) 200, 206
Cfr13I GGNCC 3 cut(s) 90, 214, 569
ClaI ATCGAT 1 cut(s) 674
CseI GACGC 1 cut(s) 353
Csp6I GTAC 4 cut(s) 252, 364, 523, 742
CviAII CATG 1 cut(s) 61
CviQI GTAC 4 cut(s) 252, 364, 523, 742
DdeI CTNAG 1 cut(s) 646
DinI GGCGCC 1 cut(s) 205
DpnI GATC 3 cut(s) 43, 277, 677
DpnII GATC 3 cut(s) 41, 275, 675
DriI GACNNNNNGTC 1 cut(s) 34
EaeI YGGCCR 2 cut(s) 47, 579
EagI CGGCCG 1 cut(s) 47
Eam1105I GACNNNNNGTC 1 cut(s) 34
Ecl136II GAGCTC 1 cut(s) 82
EclXI CGGCCG 1 cut(s) 47
Eco24I GRGCYC 2 cut(s) 77, 84
Eco31I GGTCTC 1 cut(s) 763
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 2 cut(s) 90, 569
Eco47III AGCGCT 1 cut(s) 725
Eco52I CGGCCG 1 cut(s) 47
Eco53kI GAGCTC 1 cut(s) 82
Eco88I CYCGRG 2 cut(s) 68, 77
EcoICRI GAGCTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 633
EcoRV GATATC 1 cut(s) 775
EcoT38I GRGCYC 2 cut(s) 77, 84
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 1 cut(s) 64
FaiI YATR 4 cut(s) 62, 224, 658, 702
FaqI GGGAC 1 cut(s) 555
FatI CATG 1 cut(s) 60
FauI CCCGC 2 cut(s) 366, 455
Fnu4HI GCNGC 4 cut(s) 50, 153, 537, 753
FokI GGATG 2 cut(s) 83, 369
FriOI GRGCYC 2 cut(s) 77, 84
Fsp4HI GCNGC 4 cut(s) 50, 153, 537, 753
FspBI CTAG 2 cut(s) 270, 459
GlaI GCGC 3 cut(s) 205, 451, 725
GluI GCNGC 4 cut(s) 50, 153, 537, 753
HaeII RGCGCY 2 cut(s) 207, 727
HaeIII GGCC 4 cut(s) 49, 215, 549, 581
HapII CCGG 6 cut(s) 19, 39, 46, 201, 207, 582
HgaI GACGC 1 cut(s) 353
HhaI GCGC 3 cut(s) 206, 452, 726
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 1 cut(s) 64
Hin6I GCGC 3 cut(s) 204, 450, 724
HinP1I GCGC 3 cut(s) 204, 450, 724
HinfI GANTC 3 cut(s) 35, 479, 616
HpaII CCGG 6 cut(s) 19, 39, 46, 201, 207, 582
HphI GGTGA 3 cut(s) 406, 484, 508
Hpy166II GTNNAC 1 cut(s) 525
Hpy188I TCNGA 3 cut(s) 325, 615, 812
Hpy188III TCNNGA 2 cut(s) 39, 70
Hpy8I GTNNAC 1 cut(s) 525
Hpy99I CGWCG 3 cut(s) 152, 164, 740
HpyAV CCTTC 5 cut(s) 103, 253, 265, 460, 583
HpyCH4III ACNGT 2 cut(s) 160, 496
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 2 cut(s) 539, 794
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 212
HpyF3I CTNAG 1 cut(s) 646
HpySE526I ACGT 1 cut(s) 30
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 1 cut(s) 64
HspAI GCGC 3 cut(s) 204, 450, 724
KasI GGCGCC 1 cut(s) 203
Kpn2I TCCGGA 1 cut(s) 38
KpnI GGTACC 1 cut(s) 255
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 3 cut(s) 41, 275, 675
LmnI GCTCC 3 cut(s) 6, 87, 584
Lsp1109I GCAGC 2 cut(s) 523, 739
LweI GCATC 3 cut(s) 54, 391, 680
MaeI CTAG 2 cut(s) 270, 459
MaeII ACGT 1 cut(s) 30
MalI GATC 3 cut(s) 43, 277, 677
MbiI CCGCTC 1 cut(s) 52
MboI GATC 3 cut(s) 41, 275, 675
MboII GAAGA 2 cut(s) 181, 619
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 3 cut(s) 77, 84, 359
MluCI AATT 4 cut(s) 261, 312, 391, 507
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 2 cut(s) 44, 488
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 5 cut(s) 227, 239, 372, 407, 539
MroI TCCGGA 1 cut(s) 38
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 2 cut(s) 87, 361
MspI CCGG 6 cut(s) 19, 39, 46, 201, 207, 582
MspR9I CCNGG 2 cut(s) 46, 635
MvaI CCWGG 1 cut(s) 635
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 212
NaeI GCCGGC 1 cut(s) 208
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 1 cut(s) 46
NdeII GATC 3 cut(s) 41, 275, 675
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 1 cut(s) 64
NlaIV GGNNCC 5 cut(s) 8, 43, 205, 253, 571
OliI CACNNNNGTG 1 cut(s) 497
PaeR7I CTCGAG 1 cut(s) 68
PasI CCCWGGG 1 cut(s) 634
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 156
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 616
PkrI GCNGC 4 cut(s) 51, 154, 538, 754
PleI GAGTC 2 cut(s) 43, 487
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 2 cut(s) 43, 487
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 633
PspGI CCWGG 1 cut(s) 633
PspN4I GGNNCC 5 cut(s) 8, 43, 205, 253, 571
PspPI GGNCC 3 cut(s) 90, 214, 569
PsuI RGATCY 1 cut(s) 41
RsaI GTAC 4 cut(s) 253, 365, 524, 743
RsaNI GTAC 4 cut(s) 252, 364, 523, 742
RseI CAYNNNNRTG 1 cut(s) 497
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 4 cut(s) 50, 153, 537, 753
Sau3AI GATC 3 cut(s) 41, 275, 675
Sau96I GGNCC 3 cut(s) 90, 214, 569
SchI GAGTC 2 cut(s) 44, 488
ScrFI CCNGG 2 cut(s) 46, 635
SduI GDGCHC 3 cut(s) 77, 84, 359
SfaNI GCATC 3 cut(s) 54, 391, 680
SfoI GGCGCC 1 cut(s) 205
Sfr274I CTCGAG 1 cut(s) 68
SgrAI CRCCGGYG 1 cut(s) 200
SinI GGWCC 2 cut(s) 90, 569
SlaI CTCGAG 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 4 cut(s) 261, 312, 391, 507
SsiI CCGC 7 cut(s) 50, 85, 100, 153, 359, 462, 755
SspDI GGCGCC 1 cut(s) 203
SspMI CTAG 2 cut(s) 270, 459
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 44, 633
TaaI ACNGT 2 cut(s) 160, 496
TaiI ACGT 1 cut(s) 33
TaqI TCGA 3 cut(s) 69, 274, 674
TasI AATT 4 cut(s) 261, 312, 391, 507
TatI WGTACW 3 cut(s) 363, 522, 741
TauI GCSGC 2 cut(s) 52, 155
TfiI GAWTC 1 cut(s) 616
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TscAI CASTG 2 cut(s) 354, 654
TseI GCWGC 2 cut(s) 536, 752
TspDTI ATGAA 2 cut(s) 85, 235
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 2 cut(s) 354, 654
VpaK11BI GGWCC 2 cut(s) 90, 569
XhoI CTCGAG 1 cut(s) 68
XspI CTAG 2 cut(s) 270, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.