RLG00000002332

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006232
Physical Location & Seq
Reverse (-)
32288413 .. 32294351
5939 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002332

Sequence Viewer

Length: 3597 bp
ATGTCACTCGTACAGTTGAGTGGCTTACTATCCAAAATAAGCAATGTGAGTCAGTTGAGGCAGCTCCATGCCCACCTCATTCAAAATTCCCTCCACCACCAAAACCAATGGGTGTCATTGCTCATCAACCAATGCACGCGCCTCCGTGCACCGCCGCCCTATACCCGTCTCATCTTCGACTCTACGCCTCGCCCCAACATCCATGTCTTCACCTCCATGCTCAAATATTACGCCCCTTTGGGCAATGTTTGCTCTAATCAAGTTGTTTCACTATATCAACTGATGCAGCGTTGCGAGGACTTATGCCTTGGTACCTTTGTGTACCCAGTTTTGATCAAATCGTCGGGCAGTGCCGGCATTGCGTTCCACGCGCATGTGGTGAAGTTGGGTCTTGGTTCTGATCATTATGTTCGCAATGCCATCATGAGTGTGTATTCGAAATACGGGCCGGTTGAGCATGCCCGAGACGTGTTCGATGAAATGCCTGAGAGGAGTCTTGTGGATTGGAACAACATGATTTCTGGGTATTGGAAGTGGGGGAATAAAGTTGAAGCGTGTAAACTGTTTGATATGATGCCTGAGAAGAGTGTTGTTACTTGGACTGCTATGGTTACCGGGTACGCTAGGATGAAGGATTTGGAGAATGCAAGAAGATATTTTGATAATATTCCGGAGAAAAATGTGGTGTCGTGGAACGCAATGCTCTCGGCTTATGCTCAGAATGGATCTCCAGAGGAGGCTCTGAGACTGTTTGATGGCATGATGGATTCTGGAGATAAACCAAATGAAACTACATGGGCAATTGTCATTTCAGCATGTTCGTCATGTGGTAATAGTTCCATTGCAGATTCGTTTATCCAAAAGCTCAACCAGAAGCGGATGCATTTAAGTTATTTTGGCAAAACAGCGATGCTTGATATGTATGCAAAGCGCGGGAGCATCAAGAGTGCTAGAAAAGTTTTTGATGAATTGGGGGTGTATAAGAGTTCTGGGACATGGAATGCCATGATATCAGCATATGCAAGACTTGGGAATTTGAATTCAGCTAGAGAGCTCTTTGATAAGATGCCGGAAAGAGATGTTGTCTCATGGAACTCGATGATTTCTGGTTATGCTCAGAATGGACAGTCCGCTCTTGCAATTGATCTCTTTAAAGATATGACTGCTGCTGATAACCCCAAACCAAATGAAGTCACTATGGTCAGTGTCATCTCAGCATGTGGGCATCTTGGGGCATTGGAAATAGGTAATTGGGCCGTCAACTTTCTCACAAAACATCATACCAATCTACGTATATCAGTATACAATTCTTTGATATTCTTGTACTCTAAATGTGGAAGCATGGATGATGCGAAGAGAATATTCAACGAAATGAAAAAACGAGATGTGGTTTCGTACAATACATTGATTTCAGGGTTTGCGGCTCATGGTCATGGAATGGAAGCTGTCAAGCTAATGATGAAGATGAAAGAAGAATTTATTGAGCCAAACCGTGAAACGTATATTGGTATTCTGACAGCGTGCAGCCATGCTGGATTGTTAGAAGAAGGTTGGAAGATTTTTGGGTCGATCAAAGATCCTGATGTAGATCATTATGCATGTGTTGTTGACTTATTAGGTCGCGTGGGTAAAGTGGATGAGGCAAAAAAAATAGTTGATGGCATGCAGAAAGAGCCACATGCTGGAGTTTATGGATCTCTTTTAAATGCTAGCCGGATTCACAAAAGAATTGATCTTGGGGAGTTTGCTGCAAGCAAGCTCTCTGAGCTTGAACCACATAATTCAGGAAACTACATTTTGCTTTCGAACATGTATGCCTCTGCAGGCAGGTGGGATGATGTGGTGAGAGTTAGAGAAGCAATGAGAAATGCAGGTGTGAAGAAGGCAACTGGTTGGAGTTGGGTGGAATACAATGGTAAGATGCACAAGTTCATTGCGGGAGACAGATCACACGAGCTGTCAGATGAAATTTATAGGCGATTGGCAGAACTAGGGAGGAAGCTGAGGAATGCTGGCTACATTGCTGATAAGGCCTGTGTGCTAAGAGATGTTGATGAGGAAGAGAAAGAAGAGATGGTGGGTACTCATAGTGAAAAATTGGCGGTATGCTTCGCTCTTGTTGTTACAGAAGCAAAGTCAGTGATTAGAGTTGTGAAGAATTTGAGGGTGTGCAGGGATTGCCATACAGCTATTAAGATGATATCCAAGTTGGAAGGAAGGAAAATTATTTTGAGGGATAATAACAGGTTTCACCATTTCAGTGATGGCCAATGTTCTTGTGGAGACTATTGGGGCTCTAGATTTGCTGCAATGCGAGATGTAGGCTCTGCAATTGTTGCTTCTCTCGAAAGGACTTCAGTTTGGCCTTTTCCATCTACTTGCACCATGTCAAGCTTGGAGAAGCCACTTGTTTTTGATAGGATTCAGGAATTCTCTTCTAGTGTTTGCAGGTCAAGAAGAGCAGATGAAGTTGAAGAAGGAACAAGTTGTAGCACATCCAATAGCCGCGAAGAAGAGTATACAAGAGACTACTTCTCTTTCAGCAAAAAGACTTTGAGCTTTGGCAGGAATCACAAGGCTTTCAGAAATGGTTTTGAATCATGGTTCTTGCCAGATGATCAATATTACTCCAAGACCAAGTGCAATGGAAAAGATGCAACACGTAAGTTCCTGAACGGAATAGCATTACCAAAGTTTGTTAAGATAGTAGAAGTAGGTCCAAGAGACGGTCTACAAAATGAGAAGAAGATCGTATCTGCAGCTATAAAGATTGAGCTGATTCATAGGCTGATTTCTTCTGGGTTGTCTGTTGTTGAGGCTACAAGTTTTGTATCACCCAAATGGGTTCCTCAGCTAGCAGATGCAAAGGATGTAATGGAAGCGGTTCGCAATCTCGAGGGTATTGCTACATTGCCTGTTCTGACACCTAATTTGAAAGGATTTGAAGCAGCTGTTGCAGCTGGTGCTAAGGTCGTAGCAGTCTTTGCATCAGCTTCCGAGTCATTTTCAAAGGCGAACATCAATTGTAGCATAGAAGAAAGTCTTGTTCGTTACCGAGCTGTTACTCGCGCTGCAAAGGAGCTTTCCATTCCTGTTCGAGGGTATGTATCATGTGCCATTGGATGTCCAGAAGAGGGAGCAATTCCCCCATCAAAAGTGGCATATGTAGCAAAACAACTCTATGACATGGGCTGCTTTGAAATTTCTCTTGCCGACACGACCGGAGTTGGTGCCCCAACGACGGTTGTGCCAATGCTTGAAGCTGTGATGGCTGTTGTTCCTGTTGAGAAGCTAGCTGTTCATTTCCATGATACGTACGGGCATTCCCTTCCAAATATTCTAGCCTCTCTTCAAATGGGGATTGGCACAGTCGACTCCTCTGTTGCAGGCTTAGGTGGGTGTCCATATGCCAAGGGAGCTTCCGGTAACGTTGCCACTGAAGATGTTGTATACATGCTTAGTGAACTTGGCGTGGAAACCAATGTGGATTTGGCCAAGCTCCTGGTGGCTGGTGATTTCATCAGCAGGCATTTAGGTCGCCCGTCTGGTTCAAAGATCGCCATTGCCTCAAGTCCTGTTAAAGCTGATGACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

1199

Amino Acids

132.48

Weight (kDa)

8.4

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 226 - 274 9.8e-13 PPR repeat family
PPR PF01535 229 - 258 2e-08 PPR repeat
PPR PF01535 332 - 360 4.1e-08 PPR repeat
PPR_2 PF13041 359 - 408 2.3e-10 PPR repeat family
PPR PF01535 362 - 388 6e-07 PPR repeat
PPR_2 PF13041 433 - 463 3.5e-06 PPR repeat family
PPR PF01535 434 - 462 4.4e-07 PPR repeat
PPR_2 PF13041 461 - 509 1.5e-11 PPR repeat family
PPR_3 PF13812 461 - 508 4.1e-07 Pentatricopeptide repeat domain
PPR PF01535 464 - 492 1e-05 PPR repeat
E_motif PF20431 574 - 636 5.3e-22 E motif
DYW_deaminase PF14432 672 - 764 1.6e-39 DYW family of nucleic acid deaminases
HMGL-like PF00682 900 - 1173 1.1e-54 HMGL-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1818, 1862
Acc36I ACCTGC 3 cut(s) 1818, 1862, 2439
Acc65I GGTACC 1 cut(s) 311
AccB1I GGYRCC 2 cut(s) 311, 3230
AccB7I CCANNNNNTGG 1 cut(s) 1682
AccBSI CCGCTC 1 cut(s) 1133
AccI GTMKAC 5 cut(s) 1302, 2519, 2731, 3372, 3450
AccII CGCG 6 cut(s) 139, 371, 933, 1623, 2508, 3069
AccIII TCCGGA 1 cut(s) 670
AclI AACGTT 1 cut(s) 3429
AclWI GGATC 3 cut(s) 733, 1571, 1702
AcoI YGGCCR 2 cut(s) 2266, 3492
AcsI RAATTY 8 cut(s) 85, 1033, 1039, 1475, 1968, 2158, 2429, 3201
AcuI CTGAAG 2 cut(s) 2340, 3459
AfaI GTAC 8 cut(s) 12, 313, 323, 620, 1325, 1397, 2083, 3317
AfiI CCNNNNNNNGG 5 cut(s) 1682, 2726, 3098, 3134, 3241
AflIII ACRYGT 3 cut(s) 468, 1809, 2660
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 1 cut(s) 3501
AjuI GAANNNNNNNTTGG 2 cut(s) 1488, 1520
Alw21I GWGCWC 2 cut(s) 151, 1056
Alw26I GTCTC 8 cut(s) 173, 459, 739, 1090, 1935, 2277, 2520, 2718
Alw44I GTGCAC 1 cut(s) 147
AlwI GGATC 3 cut(s) 733, 1571, 1702
Ama87I CYCGRG 2 cut(s) 462, 2894
Aor13HI TCCGGA 1 cut(s) 670
AoxI GGCC 6 cut(s) 446, 1254, 2031, 2266, 2363, 3492
ApaLI GTGCAC 1 cut(s) 147
ApoI RAATTY 8 cut(s) 85, 1033, 1039, 1475, 1968, 2158, 2429, 3201
ArsI GACNNNNNNTTYG 2 cut(s) 2914, 2946
Asp700I GAANNNNTTC 1 cut(s) 2883
Asp718I GGTACC 1 cut(s) 311
AspLEI GCGC 4 cut(s) 141, 373, 933, 3071
AspS9I GGNCC 3 cut(s) 446, 1254, 2717
AsuC2I CCSGG 1 cut(s) 616
AsuHPI GGTGA 6 cut(s) 202, 391, 1855, 2243, 2826, 3524
AsuII TTCGAA 2 cut(s) 437, 1805
AsuNHI GCTAGC 3 cut(s) 1709, 2854, 3292
AvaI CYCGRG 2 cut(s) 462, 2894
AvaII GGWCC 1 cut(s) 2717
BaeGI GKGCMC 2 cut(s) 151, 3235
BaeI ACNNNNGTAYC 2 cut(s) 303, 336
BalI TGGCCA 2 cut(s) 2268, 3494
BanI GGYRCC 2 cut(s) 311, 3230
BanII GRGCYC 2 cut(s) 1056, 2297
BauI CACGAG 1 cut(s) 1952
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 2 cut(s) 151, 1056
BbvCI CCTCAGC 2 cut(s) 2003, 2850
BccI CCATC 9 cut(s) 428, 749, 757, 1652, 2068, 2258, 2380, 3157, 3262
BceAI ACGGC 1 cut(s) 1241
BcgI CGANNNNNNTGC 8 cut(s) 687, 721, 2885, 2919, 3229, 3263, 3518, 3552
BciT130I CCWGG 1 cut(s) 3503
BclI TGATCA 3 cut(s) 333, 400, 2617
BcnI CCSGG 1 cut(s) 616
BcoDI GTCTC 8 cut(s) 173, 459, 739, 1090, 1935, 2277, 2520, 2718
BfmI CTRYAG 2 cut(s) 1821, 2757
BfuAI ACCTGC 3 cut(s) 1818, 1862, 2439
Bme1390I CCNGG 2 cut(s) 616, 3503
Bme18I GGWCC 1 cut(s) 2717
BmeT110I CYCGRG 2 cut(s) 462, 2894
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 3 cut(s) 446, 1254, 2717
BmiI GGNNCC 3 cut(s) 313, 2847, 3232
BmrFI CCNGG 2 cut(s) 616, 3503
BmrI ACTGGG 1 cut(s) 320
BmtI GCTAGC 3 cut(s) 1713, 2858, 3296
BmuI ACTGGG 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 199
BpmI CTGGAG 3 cut(s) 714, 792, 1704
Bpu10I CCTNAGC 4 cut(s) 2003, 2850, 2967, 3391
Bpu14I TTCGAA 2 cut(s) 437, 1805
BpuEI CTTGAG 1 cut(s) 3553
BpuMI CCSGG 1 cut(s) 616
BsaAI YACGTR 3 cut(s) 1292, 2663, 3315
BsaBI GATNNNNATC 1 cut(s) 1587
BsaJI CCNNGG 2 cut(s) 307, 3411
BsaWI WCCGGW 3 cut(s) 670, 3221, 3422
Bsc4I CCNNNNNNNGG 5 cut(s) 1682, 2726, 3098, 3134, 3241
Bse118I RCCGGY 2 cut(s) 353, 448
Bse1I ACTGG 2 cut(s) 326, 1894
Bse8I GATNNNNATC 1 cut(s) 1587
BseAI TCCGGA 1 cut(s) 670
BseBI CCWGG 1 cut(s) 3503
BseDI CCNNGG 2 cut(s) 307, 3411
BseGI GGATG 9 cut(s) 198, 633, 885, 1351, 1642, 1840, 2495, 2875, 3128
BseJI GATNNNNATC 1 cut(s) 1587
BseLI CCNNNNNNNGG 5 cut(s) 1682, 2726, 3098, 3134, 3241
BseMII CTCAG 9 cut(s) 477, 570, 731, 734, 1130, 1227, 1755, 1994, 2864
BseNI ACTGG 2 cut(s) 326, 1894
BseRI GAGGAG 3 cut(s) 505, 749, 3367
BseSI GKGCMC 2 cut(s) 151, 3235
BsgI GTGCAG 2 cut(s) 1543, 2191
Bsh1236I CGCG 6 cut(s) 139, 371, 933, 1623, 2508, 3069
Bsh1285I CGRYCG 1 cut(s) 3222
BshFI GGCC 6 cut(s) 448, 1256, 2033, 2268, 2365, 3494
BshNI GGYRCC 2 cut(s) 311, 3230
BsiEI CGRYCG 1 cut(s) 3222
BsiHKAI GWGCWC 2 cut(s) 151, 1056
BsiHKCI CYCGRG 2 cut(s) 462, 2894
BsiSI CCGG 8 cut(s) 354, 449, 615, 671, 1070, 1714, 3222, 3423
BsiWI CGTACG 1 cut(s) 3315
BslFI GGGAC 1 cut(s) 1006
BslI CCNNNNNNNGG 5 cut(s) 1682, 2726, 3098, 3134, 3241
BsmAI GTCTC 8 cut(s) 173, 459, 739, 1090, 1935, 2277, 2520, 2718
BsmBI CGTCTC 3 cut(s) 173, 459, 2718
BsmFI GGGAC 1 cut(s) 1006
BsmI GAATGC 4 cut(s) 649, 1006, 2014, 3322
BsnI GGCC 6 cut(s) 448, 1256, 2033, 2268, 2365, 3494
BsoBI CYCGRG 2 cut(s) 462, 2894
Bsp119I TTCGAA 2 cut(s) 437, 1805
Bsp1286I GDGCHC 4 cut(s) 151, 1056, 2297, 3235
Bsp13I TCCGGA 1 cut(s) 670
BspANI GGCC 6 cut(s) 448, 1256, 2033, 2268, 2365, 3494
BspCNI CTCAG 9 cut(s) 478, 571, 730, 735, 1129, 1226, 1756, 1995, 2863
BspEI TCCGGA 1 cut(s) 670
BspFNI CGCG 6 cut(s) 139, 371, 933, 1623, 2508, 3069
BspHI TCATGA 1 cut(s) 423
BspLI GGNNCC 3 cut(s) 313, 2847, 3232
BspMAI CTGCAG 2 cut(s) 1825, 2761
BspMI ACCTGC 3 cut(s) 1818, 1862, 2439
BspOI GCTAGC 3 cut(s) 1713, 2858, 3296
BspPI GGATC 3 cut(s) 733, 1571, 1702
BspQI GCTCTTC 1 cut(s) 2452
BspT104I TTCGAA 2 cut(s) 437, 1805
BspT107I GGYRCC 2 cut(s) 311, 3230
BsrBI CCGCTC 1 cut(s) 1133
BsrFI RCCGGY 2 cut(s) 353, 448
BsrI ACTGG 2 cut(s) 326, 1894
BssAI RCCGGY 2 cut(s) 353, 448
BssECI CCNNGG 2 cut(s) 307, 3411
BssNAI GTATAC 3 cut(s) 1303, 2520, 3451
BssSI CACGAG 1 cut(s) 1952
BssT1I CCWWGG 2 cut(s) 307, 3411
Bst1107I GTATAC 3 cut(s) 1303, 2520, 3451
Bst2BI CACGAG 1 cut(s) 1952
Bst2UI CCWGG 1 cut(s) 3503
Bst4CI ACNGT 8 cut(s) 15, 564, 750, 1128, 1493, 2729, 3244, 3370
Bst6I CTCTTC 9 cut(s) 578, 1349, 2055, 2064, 2440, 2452, 2508, 3126, 3354
BstAPI GCANNNNNTGC 6 cut(s) 249, 2178, 2336, 2954, 2963, 2984
BstBAI YACGTR 3 cut(s) 1292, 2663, 3315
BstBI TTCGAA 2 cut(s) 437, 1805
BstEII GGTNACC 1 cut(s) 610
BstENI CCTNNNNNAGG 1 cut(s) 3096
BstF5I GGATG 9 cut(s) 198, 633, 885, 1351, 1642, 1840, 2495, 2875, 3128
BstFNI CGCG 6 cut(s) 139, 371, 933, 1623, 2508, 3069
BstHHI GCGC 4 cut(s) 141, 373, 933, 3071
BstMAI GTCTC 8 cut(s) 173, 459, 739, 1090, 1935, 2277, 2520, 2718
BstMCI CGRYCG 1 cut(s) 3222
BstNI CCWGG 1 cut(s) 3503
BstNSI RCATGY 9 cut(s) 377, 461, 819, 1221, 1602, 1666, 1682, 1813, 3457
BstPI GGTNACC 1 cut(s) 610
BstSCI CCNGG 2 cut(s) 614, 3501
BstSFI CTRYAG 2 cut(s) 1821, 2757
BstSLI GKGCMC 2 cut(s) 151, 3235
BstSNI TACGTA 2 cut(s) 1292, 3315
BstUI CGCG 6 cut(s) 139, 371, 933, 1623, 2508, 3069
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 3 cut(s) 725, 1576, 1694
BstXI CCANNNNNNTGG 1 cut(s) 3502
BstYI RGATCY 3 cut(s) 725, 1576, 1694
BstZ17I GTATAC 3 cut(s) 1303, 2520, 3451
BsuRI GGCC 6 cut(s) 448, 1256, 2033, 2268, 2365, 3494
BtgZI GCGATG 1 cut(s) 923
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 9 cut(s) 198, 633, 885, 1351, 1642, 1840, 2495, 2875, 3128
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 5 cut(s) 355, 1210, 2145, 2266, 3435
BveI ACCTGC 3 cut(s) 1818, 1862, 2439
CciI TCATGA 1 cut(s) 423
CfoI GCGC 4 cut(s) 141, 373, 933, 3071
Cfr10I RCCGGY 2 cut(s) 353, 448
Cfr13I GGNCC 3 cut(s) 446, 1254, 2717
Csp6I GTAC 8 cut(s) 11, 312, 322, 619, 1324, 1396, 2082, 3316
CviQI GTAC 8 cut(s) 11, 312, 322, 619, 1324, 1396, 2082, 3316
DraI TTTAAA 2 cut(s) 1153, 1704
EaeI YGGCCR 2 cut(s) 2266, 3492
Eam1104I CTCTTC 9 cut(s) 578, 1349, 2055, 2064, 2440, 2452, 2508, 3126, 3354
EarI CTCTTC 9 cut(s) 578, 1349, 2055, 2064, 2440, 2452, 2508, 3126, 3354
Ecl136II GAGCTC 1 cut(s) 1054
Eco105I TACGTA 2 cut(s) 1292, 3315
Eco130I CCWWGG 2 cut(s) 307, 3411
Eco147I AGGCCT 1 cut(s) 2033
Eco24I GRGCYC 2 cut(s) 1056, 2297
Eco32I GATATC 2 cut(s) 1011, 2202
Eco47I GGWCC 1 cut(s) 2717
Eco53kI GAGCTC 1 cut(s) 1054
Eco57I CTGAAG 2 cut(s) 2340, 3459
Eco88I CYCGRG 2 cut(s) 462, 2894
Eco91I GGTNACC 1 cut(s) 610
EcoICRI GAGCTC 1 cut(s) 1054
EcoNI CCTNNNNNAGG 1 cut(s) 3096
EcoO65I GGTNACC 1 cut(s) 610
EcoRI GAATTC 2 cut(s) 1039, 2429
EcoRII CCWGG 1 cut(s) 3501
EcoRV GATATC 2 cut(s) 1011, 2202
EcoT14I CCWWGG 2 cut(s) 307, 3411
EcoT22I ATGCAT 2 cut(s) 885, 1600
EcoT38I GRGCYC 2 cut(s) 1056, 2297
ErhI CCWWGG 2 cut(s) 307, 3411
Esp3I CGTCTC 3 cut(s) 173, 459, 2718
FalI AAGNNNNNCTT 2 cut(s) 3027, 3059
FaqI GGGAC 1 cut(s) 1006
FauI CCCGC 2 cut(s) 926, 1930
FauNDI CATATG 3 cut(s) 1018, 3163, 3406
FbaI TGATCA 3 cut(s) 333, 400, 2617
FblI GTMKAC 5 cut(s) 1302, 2519, 2731, 3372, 3450
FokI GGATG 9 cut(s) 185, 640, 892, 1358, 1649, 1847, 2482, 2882, 3135
FriOI GRGCYC 2 cut(s) 1056, 2297
GlaI GCGC 4 cut(s) 140, 372, 932, 3070
GsuI CTGGAG 3 cut(s) 714, 792, 1704
HaeIII GGCC 6 cut(s) 448, 1256, 2033, 2268, 2365, 3494
HapII CCGG 8 cut(s) 354, 449, 615, 671, 1070, 1714, 3222, 3423
HhaI GCGC 4 cut(s) 141, 373, 933, 3071
Hin6I GCGC 4 cut(s) 139, 371, 931, 3069
HinP1I GCGC 4 cut(s) 139, 371, 931, 3069
HincII GTYRAC 3 cut(s) 1261, 1609, 3373
HindII GTYRAC 3 cut(s) 1261, 1609, 3373
HindIII AAGCTT 1 cut(s) 2392
HpaII CCGG 8 cut(s) 354, 449, 615, 671, 1070, 1714, 3222, 3423
HphI GGTGA 6 cut(s) 202, 391, 1855, 2243, 2826, 3524
Hpy99I CGWCG 2 cut(s) 346, 3244
HpyAV CCTTC 7 cut(s) 625, 1541, 1876, 2207, 2211, 2471, 3338
HpyCH4III ACNGT 8 cut(s) 15, 564, 750, 1128, 1493, 2729, 3244, 3370
HpyCH4IV ACGT 6 cut(s) 468, 1291, 1499, 2662, 3314, 3429
HpySE526I ACGT 6 cut(s) 468, 1291, 1499, 2662, 3314, 3429
HspAI GCGC 4 cut(s) 139, 371, 931, 3069
Kpn2I TCCGGA 1 cut(s) 670
KpnI GGTACC 1 cut(s) 315
KroI GCCGGC 1 cut(s) 353
KroNI GCCGGC 1 cut(s) 355
Ksp22I TGATCA 3 cut(s) 333, 400, 2617
LguI GCTCTTC 1 cut(s) 2452
LmnI GCTCC 6 cut(s) 69, 936, 3079, 3137, 3416, 3504
MaeII ACGT 6 cut(s) 468, 1291, 1499, 2662, 3314, 3429
MaeIII GTNAC 8 cut(s) 3, 592, 610, 1192, 2122, 3050, 3061, 3425
MbiI CCGCTC 1 cut(s) 1133
MfeI CAATTG 4 cut(s) 801, 1140, 2331, 3022
MflI RGATCY 3 cut(s) 725, 1576, 1694
MhlI GDGCHC 4 cut(s) 151, 1056, 2297, 3235
MlsI TGGCCA 2 cut(s) 2268, 3494
MluNI TGGCCA 2 cut(s) 2268, 3494
MlyI GAGTC 6 cut(s) 58, 173, 502, 3008, 3368, 3584
MmeI TCCRAC 3 cut(s) 1532, 1874, 2190
Mox20I TGGCCA 2 cut(s) 2268, 3494
Mph1103I ATGCAT 2 cut(s) 885, 1600
MroI TCCGGA 1 cut(s) 670
MroNI GCCGGC 1 cut(s) 353
MroXI GAANNNNTTC 1 cut(s) 2883
MscI TGGCCA 2 cut(s) 2268, 3494
MseI TTAA 7 cut(s) 887, 1152, 1703, 2193, 2700, 3579, 3595
MslI CAYNNNNRTG 7 cut(s) 215, 372, 428, 1431, 2259, 2839, 3306
Msp20I TGGCCA 2 cut(s) 2268, 3494
MspA1I CMGCKG 2 cut(s) 2951, 2960
MspI CCGG 8 cut(s) 354, 449, 615, 671, 1070, 1714, 3222, 3423
MspR9I CCNGG 2 cut(s) 616, 3503
MunI CAATTG 4 cut(s) 801, 1140, 2331, 3022
Mva1269I GAATGC 4 cut(s) 649, 1006, 2014, 3322
MvaI CCWGG 1 cut(s) 3503
MvnI CGCG 6 cut(s) 139, 371, 933, 1623, 2508, 3069
NaeI GCCGGC 1 cut(s) 355
NciI CCSGG 1 cut(s) 616
NdeI CATATG 3 cut(s) 1018, 3163, 3406
NgoMIV GCCGGC 1 cut(s) 353
NheI GCTAGC 3 cut(s) 1709, 2854, 3292
NlaIV GGNNCC 3 cut(s) 313, 2847, 3232
NmeAIII GCCGAG 1 cut(s) 686
NmuCI GTSAC 2 cut(s) 3, 1192
NsiI ATGCAT 2 cut(s) 885, 1600
NspI RCATGY 9 cut(s) 377, 461, 819, 1221, 1602, 1666, 1682, 1813, 3457
NspV TTCGAA 2 cut(s) 437, 1805
PaeI GCATGC 2 cut(s) 461, 1666
PaeR7I CTCGAG 1 cut(s) 2894
PagI TCATGA 1 cut(s) 423
PaqCI CACCTGC 2 cut(s) 1818, 1862
PceI AGGCCT 1 cut(s) 2033
PciI ACATGT 1 cut(s) 1809
PciSI GCTCTTC 1 cut(s) 2452
PctI GAATGC 4 cut(s) 649, 1006, 2014, 3322
PdiI GCCGGC 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 2883
PfeI GAWTC 7 cut(s) 767, 848, 1717, 2422, 2569, 2597, 2779
Pfl23II CGTACG 1 cut(s) 3315
PflMI CCANNNNNTGG 1 cut(s) 1682
PleI GAGTC 6 cut(s) 57, 173, 501, 3007, 3368, 3584
PpsI GAGTC 6 cut(s) 57, 173, 501, 3007, 3368, 3584
Ppu21I YACGTR 3 cut(s) 1292, 2663, 3315
PscI ACATGT 1 cut(s) 1809
Psp124BI GAGCTC 1 cut(s) 1056
Psp1406I AACGTT 1 cut(s) 3429
Psp6I CCWGG 1 cut(s) 3501
PspEI GGTNACC 1 cut(s) 610
PspGI CCWGG 1 cut(s) 3501
PspLI CGTACG 1 cut(s) 3315
PspN4I GGNNCC 3 cut(s) 313, 2847, 3232
PspPI GGNCC 3 cut(s) 446, 1254, 2717
PstI CTGCAG 2 cut(s) 1825, 2761
PsuI RGATCY 3 cut(s) 725, 1576, 1694
PvuII CAGCTG 2 cut(s) 2951, 2960
RsaI GTAC 8 cut(s) 12, 313, 323, 620, 1325, 1397, 2083, 3317
RsaNI GTAC 8 cut(s) 11, 312, 322, 619, 1324, 1396, 2082, 3316
RseI CAYNNNNRTG 7 cut(s) 215, 372, 428, 1431, 2259, 2839, 3306
SacI GAGCTC 1 cut(s) 1056
SalI GTCGAC 1 cut(s) 3371
SapI GCTCTTC 1 cut(s) 2452
SaqAI TTAA 7 cut(s) 887, 1152, 1703, 2193, 2700, 3579, 3595
Sau96I GGNCC 3 cut(s) 446, 1254, 2717
SchI GAGTC 6 cut(s) 58, 173, 502, 3008, 3368, 3584
ScrFI CCNGG 2 cut(s) 616, 3503
SduI GDGCHC 4 cut(s) 151, 1056, 2297, 3235
SfcI CTRYAG 2 cut(s) 1821, 2757
Sfr274I CTCGAG 1 cut(s) 2894
SfuI TTCGAA 2 cut(s) 437, 1805
SinI GGWCC 1 cut(s) 2717
SlaI CTCGAG 1 cut(s) 2894
SmiMI CAYNNNNRTG 7 cut(s) 215, 372, 428, 1431, 2259, 2839, 3306
SmlI CTYRAG 2 cut(s) 2894, 3568
SmoI CTYRAG 2 cut(s) 2894, 3568
SnaBI TACGTA 2 cut(s) 1292, 3315
SphI GCATGC 2 cut(s) 461, 1666
SseBI AGGCCT 1 cut(s) 2033
SspI AATATT 5 cut(s) 227, 667, 1362, 2624, 3337
SstI GAGCTC 1 cut(s) 1056
StuI AGGCCT 1 cut(s) 2033
StyD4I CCNGG 2 cut(s) 614, 3501
StyI CCWWGG 2 cut(s) 307, 3411
TaaI ACNGT 8 cut(s) 15, 564, 750, 1128, 1493, 2729, 3244, 3370
TaiI ACGT 6 cut(s) 471, 1294, 1502, 2665, 3317, 3432
TatI WGTACW 1 cut(s) 1323
TauI GCSGC 3 cut(s) 157, 1424, 2508
TfiI GAWTC 7 cut(s) 767, 848, 1717, 2422, 2569, 2597, 2779
Tru1I TTAA 7 cut(s) 887, 1152, 1703, 2193, 2700, 3579, 3595
Tru9I TTAA 7 cut(s) 887, 1152, 1703, 2193, 2700, 3579, 3595
TscAI CASTG 5 cut(s) 355, 1210, 2145, 2266, 3442
TseFI GTSAC 2 cut(s) 3, 1192
Tsp45I GTSAC 2 cut(s) 3, 1192
TspGWI ACGGA 2 cut(s) 134, 2691
TspRI CASTG 5 cut(s) 355, 1210, 2145, 2266, 3442
Van91I CCANNNNNTGG 1 cut(s) 1682
VneI GTGCAC 1 cut(s) 147
VpaK11BI GGWCC 1 cut(s) 2717
XagI CCTNNNNNAGG 1 cut(s) 3096
XapI RAATTY 8 cut(s) 85, 1033, 1039, 1475, 1968, 2158, 2429, 3201
XbaI TCTAGA 1 cut(s) 2297
XceI RCATGY 9 cut(s) 377, 461, 819, 1221, 1602, 1666, 1682, 1813, 3457
XcmI CCANNNNNNNNNTGG 5 cut(s) 2261, 2276, 2392, 3487, 3502
XhoI CTCGAG 1 cut(s) 2894
XmiI GTMKAC 5 cut(s) 1302, 2519, 2731, 3372, 3450
XmnI GAANNNNTTC 1 cut(s) 2883
Zsp2I ATGCAT 2 cut(s) 885, 1600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.