RLG00000002335

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006232
Physical Location & Seq
Reverse (-)
32308205 .. 32309239
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002335

Sequence Viewer

Length: 867 bp
ATGCCTGAGAGGAGTCTTGTGGATTGGAACAACATGATTTCTGGGTATTGGAAGTGGGGGAATAAAGTTGAAGCGTGTAAACTGTTTGATATGATGCCTGAGAAGAGTGTTGTTACTTGGACTGCTATGGTTGCCGGGTACGCTAGGATGAAGGATTTGGAGAATGCAAGAAGGTATTTTGATAATATTCCGGAGAAAAATGTGGTGTCGTGGAATGTAATGCTCTCGGCTTATGCTCAGAATGGATCTCCAGAGGAGGCTTGGAGATTGTTTGATGGCATGATGATTTCTAGAGATAAACCAAATGAAACTAAATGGGCAATTGTCATTGCAGCGTGTTCGTCATGTGGTAATAGTTCCATTGCAGATTCGTTTATCCAAAAGCTCAACCGGAAGCGGATGCATTTAAGTTATTTTGGCAAAACTGCGATGCTTGATATGTATGCAAAGCGTGGGAGCATCAAGAGTGCTAGAGAAGTTTTTGATGAATTGGGGGTGTATAAGAGCTCTGGGACATGGAATGCCATGATATCAGCATATGCAAGAGTTGGGAATTTAACTTCAGCTAGAGAGCTCTTTGATAAGATGCCGGAAAGAGATGTTGTCTCTTGGAACTCGATGATATCTGGTTATGCTCAGAACGGACAGTCTGCTCTTGCAATTGATCTCTTTAAAGATATGACTGCTGCAGATAACCCCAAACCAAATGAAGTCACTATGGTCAATGTCATCTCAGCATGTGGGCATCTTGGGGCGTTGGAAATAGGGTTTGCGGCTCATGGTCATGGAATGGAAGCTGTAAAGCTAATGATGAAGATGAAAGAAGAATTTATTGAGCCAAACCGTGAAACGTATATTGGTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

289

Amino Acids

32.43

Weight (kDa)

7.63

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 9 - 36 5.9e-06 PPR repeat
PPR_2 PF13041 66 - 113 3.8e-09 PPR repeat family
PPR PF01535 69 - 95 1.3e-07 PPR repeat
PPR_2 PF13041 170 - 200 4.3e-06 PPR repeat family
PPR PF01535 172 - 200 2.4e-08 PPR repeat
PPR_2 PF13041 199 - 248 1.5e-10 PPR repeat family
PPR PF01535 202 - 228 1e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 190
AciI CCGC 2 cut(s) 397, 773
AclWI GGATC 1 cut(s) 253
AcsI RAATTY 2 cut(s) 553, 827
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 1 cut(s) 140
AgsI TTSAA 1 cut(s) 71
AjuI GAANNNNNNNTTGG 1 cut(s) 840
AluBI AGCT 6 cut(s) 385, 507, 566, 574, 797, 805
AluI AGCT 6 cut(s) 385, 507, 566, 574, 797, 805
Alw21I GWGCWC 2 cut(s) 509, 576
Alw26I GTCTC 1 cut(s) 610
AlwI GGATC 1 cut(s) 253
Aor13HI TCCGGA 1 cut(s) 190
ApeKI GCWGC 2 cut(s) 332, 686
ApoI RAATTY 2 cut(s) 553, 827
AsuC2I CCSGG 1 cut(s) 136
BanII GRGCYC 2 cut(s) 509, 576
Bbv12I GWGCWC 2 cut(s) 509, 576
BbvI GCAGC 2 cut(s) 344, 673
BccI CCATC 1 cut(s) 269
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 610
BfaI CTAG 4 cut(s) 144, 291, 471, 567
BfmI CTRYAG 1 cut(s) 687
BisI GCNGC 3 cut(s) 333, 687, 774
BlsI GCNGC 3 cut(s) 334, 688, 775
Bme1390I CCNGG 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 136
BmsI GCATC 6 cut(s) 84, 390, 420, 468, 576, 754
BpmI CTGGAG 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 136
BsaWI WCCGGW 2 cut(s) 190, 390
Bse3DI GCAATG 2 cut(s) 327, 360
BseAI TCCGGA 1 cut(s) 190
BseGI GGATG 2 cut(s) 153, 405
BseMI GCAATG 2 cut(s) 327, 360
BseMII CTCAG 4 cut(s) 90, 251, 650, 747
BseRI GAGGAG 2 cut(s) 25, 269
BseXI GCAGC 2 cut(s) 344, 673
BsiHKAI GWGCWC 2 cut(s) 509, 576
BsiSI CCGG 4 cut(s) 135, 191, 391, 590
BslFI GGGAC 1 cut(s) 526
BsmAI GTCTC 1 cut(s) 610
BsmFI GGGAC 1 cut(s) 526
BsmI GAATGC 2 cut(s) 169, 526
Bsp1286I GDGCHC 2 cut(s) 509, 576
Bsp13I TCCGGA 1 cut(s) 190
Bsp143I GATC 2 cut(s) 245, 664
BspACI CCGC 2 cut(s) 397, 773
BspCNI CTCAG 4 cut(s) 91, 250, 649, 746
BspEI TCCGGA 1 cut(s) 190
BspMAI CTGCAG 1 cut(s) 691
BspPI GGATC 1 cut(s) 253
BsrDI GCAATG 2 cut(s) 327, 360
BssMI GATC 2 cut(s) 245, 664
Bst4CI ACNGT 3 cut(s) 84, 648, 845
Bst6I CTCTTC 1 cut(s) 98
BstDEI CTNAG 5 cut(s) 6, 99, 237, 636, 733
BstF5I GGATG 2 cut(s) 153, 405
BstKTI GATC 2 cut(s) 248, 667
BstMAI GTCTC 1 cut(s) 610
BstMBI GATC 2 cut(s) 245, 664
BstMWI GCNNNNNNNGC 2 cut(s) 131, 140
BstNSI RCATGY 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 134
BstSFI CTRYAG 1 cut(s) 687
BstV1I GCAGC 2 cut(s) 344, 673
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BtgZI GCGATG 1 cut(s) 443
BtsCI GGATG 2 cut(s) 153, 405
Csp6I GTAC 1 cut(s) 139
CviAII CATG 8 cut(s) 34, 280, 345, 516, 526, 738, 779, 785
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 5 cut(s) 6, 99, 237, 636, 733
DpnI GATC 2 cut(s) 247, 666
DpnII GATC 2 cut(s) 245, 664
DraI TTTAAA 1 cut(s) 673
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Ecl136II GAGCTC 2 cut(s) 507, 574
Eco24I GRGCYC 2 cut(s) 509, 576
Eco32I GATATC 2 cut(s) 531, 624
Eco53kI GAGCTC 2 cut(s) 507, 574
Eco57I CTGAAG 1 cut(s) 546
EcoICRI GAGCTC 2 cut(s) 507, 574
EcoRV GATATC 2 cut(s) 531, 624
EcoT22I ATGCAT 1 cut(s) 405
EcoT38I GRGCYC 2 cut(s) 509, 576
FaeI CATG 8 cut(s) 37, 283, 348, 519, 529, 741, 782, 788
FaqI GGGAC 1 cut(s) 526
FatI CATG 8 cut(s) 33, 279, 344, 515, 525, 737, 778, 784
FauNDI CATATG 1 cut(s) 538
Fnu4HI GCNGC 3 cut(s) 333, 687, 774
FokI GGATG 2 cut(s) 160, 412
FriOI GRGCYC 2 cut(s) 509, 576
Fsp4HI GCNGC 3 cut(s) 333, 687, 774
FspBI CTAG 4 cut(s) 144, 291, 471, 567
GluI GCNGC 3 cut(s) 333, 687, 774
GsuI CTGGAG 1 cut(s) 234
HapII CCGG 4 cut(s) 135, 191, 391, 590
Hin1II CATG 8 cut(s) 37, 283, 348, 519, 529, 741, 782, 788
HinfI GANTC 2 cut(s) 13, 368
HpaII CCGG 4 cut(s) 135, 191, 391, 590
Hpy166II GTNNAC 1 cut(s) 80
Hpy188I TCNGA 3 cut(s) 240, 639, 866
Hpy188III TCNNGA 4 cut(s) 191, 251, 291, 463
Hpy8I GTNNAC 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 145, 165
HpyCH4III ACNGT 3 cut(s) 84, 648, 845
HpyCH4IV ACGT 1 cut(s) 851
HpyCH4V TGCA 8 cut(s) 167, 332, 365, 403, 446, 542, 659, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 140
HpyF3I CTNAG 5 cut(s) 6, 99, 237, 636, 733
HpySE526I ACGT 1 cut(s) 851
Hsp92II CATG 8 cut(s) 37, 283, 348, 519, 529, 741, 782, 788
Kpn2I TCCGGA 1 cut(s) 190
Kzo9I GATC 2 cut(s) 245, 664
LmnI GCTCC 1 cut(s) 456
Lsp1109I GCAGC 2 cut(s) 344, 673
LweI GCATC 6 cut(s) 84, 390, 420, 468, 576, 754
MaeI CTAG 4 cut(s) 144, 291, 471, 567
MaeII ACGT 1 cut(s) 851
MaeIII GTNAC 2 cut(s) 112, 712
MalI GATC 2 cut(s) 247, 666
MboI GATC 2 cut(s) 245, 664
MboII GAAGA 3 cut(s) 115, 826, 836
MfeI CAATTG 2 cut(s) 321, 660
MflI RGATCY 1 cut(s) 245
MhlI GDGCHC 2 cut(s) 509, 576
MluCI AATT 5 cut(s) 321, 488, 553, 660, 827
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 1 cut(s) 738
MnlI CCTC 3 cut(s) 3, 247, 250
Mph1103I ATGCAT 1 cut(s) 405
MroI TCCGGA 1 cut(s) 190
MseI TTAA 3 cut(s) 407, 557, 672
MslI CAYNNNNRTG 1 cut(s) 783
MspI CCGG 4 cut(s) 135, 191, 391, 590
MspR9I CCNGG 1 cut(s) 136
MunI CAATTG 2 cut(s) 321, 660
Mva1269I GAATGC 2 cut(s) 169, 526
MwoI GCNNNNNNNGC 2 cut(s) 131, 140
NciI CCSGG 1 cut(s) 136
NdeI CATATG 1 cut(s) 538
NdeII GATC 2 cut(s) 245, 664
NlaIII CATG 8 cut(s) 37, 283, 348, 519, 529, 741, 782, 788
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 1 cut(s) 712
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 1 cut(s) 741
PctI GAATGC 2 cut(s) 169, 526
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 3 cut(s) 334, 688, 775
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
Psp124BI GAGCTC 2 cut(s) 509, 576
PstI CTGCAG 1 cut(s) 691
PsuI RGATCY 1 cut(s) 245
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 783
SacI GAGCTC 2 cut(s) 509, 576
SaqAI TTAA 3 cut(s) 407, 557, 672
SatI GCNGC 3 cut(s) 333, 687, 774
Sau3AI GATC 2 cut(s) 245, 664
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 2 cut(s) 509, 576
SetI ASST 8 cut(s) 176, 387, 509, 568, 576, 799, 807, 854
SfaNI GCATC 6 cut(s) 84, 390, 420, 468, 576, 754
SfcI CTRYAG 1 cut(s) 687
SmiMI CAYNNNNRTG 1 cut(s) 783
Sse9I AATT 5 cut(s) 321, 488, 553, 660, 827
SsiI CCGC 2 cut(s) 397, 773
SspI AATATT 1 cut(s) 187
SspMI CTAG 4 cut(s) 144, 291, 471, 567
SstI GAGCTC 2 cut(s) 509, 576
StyD4I CCNGG 1 cut(s) 134
TaaI ACNGT 3 cut(s) 84, 648, 845
TaiI ACGT 1 cut(s) 854
TaqI TCGA 1 cut(s) 617
TasI AATT 5 cut(s) 321, 488, 553, 660, 827
TauI GCSGC 1 cut(s) 776
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 3 cut(s) 407, 557, 672
Tru9I TTAA 3 cut(s) 407, 557, 672
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 2 cut(s) 332, 686
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 6 cut(s) 164, 321, 501, 723, 827, 833
TspGWI ACGGA 1 cut(s) 657
XapI RAATTY 2 cut(s) 553, 827
XbaI TCTAGA 1 cut(s) 290
XceI RCATGY 1 cut(s) 741
XcmI CCANNNNNNNNNTGG 1 cut(s) 258
XspI CTAG 4 cut(s) 144, 291, 471, 567
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.