RLG00000006839

Multidrug resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004866
Physical Location & Seq
Reverse (-)
10950017 .. 10951299
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006839

Sequence Viewer

Length: 810 bp
ATGGAGCTTGTTATTAAGCAAGGTTTCATAAAGGGAGTGATGATAGGGAGCATGGGAATTGTTAATGCTACCTGGGCTTTCCCGGGTTGGATTGGTAGCATACTTGTCATTGAGAAAGGAGAGAAAGGAGGCACTGTCGTCGTTGCTAGTATCTGCATCGTCTGGAAGGACTGTAAGTCCATAATGAGTGCACTCCCAAATCTGTCTTTCTTCATGGAGGTGACATCTGTTGCTATTTGGGTGTTTGAAATGCTTGAAAGAGATCCTGCCATAGACACTATGAAAAGAGGAAAAGTATTGGCACATTTGAAAGGAAAAGTGAAGTTTCAAGAGGTGCACTTTAGTTACCCATCAAGACCTGATACCCCAGTTCTTGAAGGACTCAATCTTACCGTACGAGCTAGAAAGACTGTCAGTTTTGTTGGAGCAATGTGGTTCAGATCCCAGATGGGACTTGTTAATCAAGAACTAGTTCTCTTTGCAACATCTATAAAAGAAAACATTATATTCGGAAAGGAAGGAGCTTCGATGGAAAATGTCACAGATGCAGCCAAGGCAGCAAATGCTCATGACTTCATTGTTAAACTATCCGAAGGATATGAAACTCAATTGTCTGGAGGGCAAAAGCAAAGTGGTGCTATAGCAATGCCTCTCCTTAGAAATCCCATAATCCTTCTACTTGACGAAGCCACAAGCGCTTTCGATGCACAATCCAAAAGAGTAGTACAAGAAGCCCAGGACGTGGCTTTAGTAGGATGGACAACTCCATTGTTGCTCATCGGCATTCCACAATATGCAAAGCTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

270

Amino Acids

29.39

Weight (kDa)

8.45

Isoelectric Point (pI)

25.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 145 - 232 3e-10 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 742
AclWI GGATC 2 cut(s) 257, 435
AfaI GTAC 2 cut(s) 396, 726
AfeI AGCGCT 1 cut(s) 697
AfiI CCNNNNNNNGG 1 cut(s) 742
AgsI TTSAA 5 cut(s) 248, 257, 310, 329, 377
AhdI GACNNNNNGTC 1 cut(s) 175
AhlI ACTAGT 1 cut(s) 469
AjiI CACGTC 1 cut(s) 742
AjnI CCWGG 2 cut(s) 71, 735
AluBI AGCT 4 cut(s) 7, 401, 524, 802
AluI AGCT 4 cut(s) 7, 401, 524, 802
Alw21I GWGCWC 2 cut(s) 193, 339
Alw44I GTGCAC 2 cut(s) 189, 335
AlwI GGATC 2 cut(s) 257, 435
Ama87I CYCGRG 1 cut(s) 82
Aor51HI AGCGCT 1 cut(s) 697
ApaLI GTGCAC 2 cut(s) 189, 335
ApeKI GCWGC 2 cut(s) 548, 557
Asp700I GAANNNNTTC 1 cut(s) 471
AspLEI GCGC 1 cut(s) 698
AsuC2I CCSGG 2 cut(s) 83, 84
AsuHPI GGTGA 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 82
BaeGI GKGCMC 2 cut(s) 193, 339
BaeI ACNNNNGTAYC 2 cut(s) 354, 387
Bbv12I GWGCWC 2 cut(s) 193, 339
BbvI GCAGC 2 cut(s) 560, 569
BccI CCATC 4 cut(s) 358, 442, 523, 750
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciT130I CCWGG 2 cut(s) 73, 737
BcnI CCSGG 2 cut(s) 83, 84
BcuI ACTAGT 1 cut(s) 469
BfaI CTAG 3 cut(s) 147, 402, 470
BfmI CTRYAG 1 cut(s) 639
BfoI RGCGCY 1 cut(s) 699
BisI GCNGC 2 cut(s) 549, 558
BlsI GCNGC 2 cut(s) 550, 559
Bme1390I CCNGG 4 cut(s) 73, 83, 84, 737
BmeRI GACNNNNNGTC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 82
BmgBI CACGTC 1 cut(s) 742
BmrFI CCNGG 4 cut(s) 73, 83, 84, 737
BmrI ACTGGG 1 cut(s) 362
BmsI GCATC 3 cut(s) 165, 535, 694
BmuI ACTGGG 1 cut(s) 362
BpmI CTGGAG 1 cut(s) 636
BpuMI CCSGG 2 cut(s) 83, 84
BsaJI CCNNGG 4 cut(s) 72, 82, 552, 735
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 1 cut(s) 742
Bse1I ACTGG 1 cut(s) 368
Bse3DI GCAATG 2 cut(s) 435, 651
BseBI CCWGG 2 cut(s) 73, 737
BseDI CCNNGG 4 cut(s) 72, 82, 552, 735
BseGI GGATG 1 cut(s) 761
BseLI CCNNNNNNNGG 1 cut(s) 742
BseMI GCAATG 2 cut(s) 435, 651
BseMII CTCAG 1 cut(s) 794
BseNI ACTGG 1 cut(s) 368
BseSI GKGCMC 2 cut(s) 193, 339
BseXI GCAGC 2 cut(s) 560, 569
BsiHKAI GWGCWC 2 cut(s) 193, 339
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 1 cut(s) 83
BsiWI CGTACG 1 cut(s) 394
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 1 cut(s) 742
BsmFI GGGAC 1 cut(s) 465
BsmI GAATGC 1 cut(s) 783
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 2 cut(s) 193, 339
Bsp143I GATC 2 cut(s) 262, 440
BspCNI CTCAG 1 cut(s) 795
BspHI TCATGA 1 cut(s) 568
BspPI GGATC 2 cut(s) 257, 435
BsrDI GCAATG 2 cut(s) 435, 651
BsrI ACTGG 1 cut(s) 368
BssECI CCNNGG 4 cut(s) 72, 82, 552, 735
BssMI GATC 2 cut(s) 262, 440
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 73, 737
Bst4CI ACNGT 4 cut(s) 136, 173, 394, 412
BstAPI GCANNNNNTGC 1 cut(s) 563
BstDEI CTNAG 2 cut(s) 656, 803
BstF5I GGATG 1 cut(s) 761
BstH2I RGCGCY 1 cut(s) 699
BstHHI GCGC 1 cut(s) 698
BstKTI GATC 2 cut(s) 265, 443
BstMBI GATC 2 cut(s) 262, 440
BstMWI GCNNNNNNNGC 6 cut(s) 74, 554, 557, 563, 695, 704
BstNI CCWGG 2 cut(s) 73, 737
BstSCI CCNGG 4 cut(s) 71, 81, 82, 735
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 2 cut(s) 193, 339
BstV1I GCAGC 2 cut(s) 560, 569
BstX2I RGATCY 2 cut(s) 262, 440
BstYI RGATCY 2 cut(s) 262, 440
BtrI CACGTC 1 cut(s) 742
BtsCI GGATG 1 cut(s) 761
BtsIMutI CAGTG 1 cut(s) 132
CciI TCATGA 1 cut(s) 568
CfoI GCGC 1 cut(s) 698
Cfr9I CCCGGG 1 cut(s) 82
Csp6I GTAC 2 cut(s) 395, 725
CviAII CATG 3 cut(s) 52, 214, 569
CviJI RGCY 9 cut(s) 7, 77, 401, 524, 551, 689, 734, 746, 802
CviKI_1 RGCY 9 cut(s) 7, 77, 401, 524, 551, 689, 734, 746, 802
CviQI GTAC 2 cut(s) 395, 725
DdeI CTNAG 2 cut(s) 656, 803
DpnI GATC 2 cut(s) 264, 442
DpnII GATC 2 cut(s) 262, 440
DriI GACNNNNNGTC 1 cut(s) 175
Eam1105I GACNNNNNGTC 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 552
Eco47III AGCGCT 1 cut(s) 697
Eco88I CYCGRG 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 71, 735
EcoT14I CCWWGG 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 3 cut(s) 55, 217, 572
FaqI GGGAC 1 cut(s) 465
FatI CATG 3 cut(s) 51, 213, 568
Fnu4HI GCNGC 2 cut(s) 549, 558
FokI GGATG 1 cut(s) 768
Fsp4HI GCNGC 2 cut(s) 549, 558
FspBI CTAG 3 cut(s) 147, 402, 470
GlaI GCGC 1 cut(s) 697
GluI GCNGC 2 cut(s) 549, 558
GsuI CTGGAG 1 cut(s) 636
HaeII RGCGCY 1 cut(s) 699
HapII CCGG 1 cut(s) 83
HhaI GCGC 1 cut(s) 698
Hin1II CATG 3 cut(s) 55, 217, 572
Hin6I GCGC 1 cut(s) 696
HinP1I GCGC 1 cut(s) 696
HinfI GANTC 1 cut(s) 381
HpaII CCGG 1 cut(s) 83
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 2 cut(s) 191, 337
Hpy188I TCNGA 3 cut(s) 440, 512, 592
Hpy188III TCNNGA 7 cut(s) 163, 329, 354, 374, 464, 569, 615
Hpy8I GTNNAC 2 cut(s) 191, 337
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 5 cut(s) 160, 371, 512, 587, 683
HpyCH4III ACNGT 4 cut(s) 136, 173, 394, 412
HpyCH4IV ACGT 1 cut(s) 741
HpyCH4V TGCA 7 cut(s) 156, 191, 337, 482, 548, 707, 797
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 554, 557, 563, 695, 704
HpyF3I CTNAG 2 cut(s) 656, 803
HpySE526I ACGT 1 cut(s) 741
Hsp92II CATG 3 cut(s) 55, 217, 572
HspAI GCGC 1 cut(s) 696
Kzo9I GATC 2 cut(s) 262, 440
LmnI GCTCC 4 cut(s) 4, 48, 425, 521
Lsp1109I GCAGC 2 cut(s) 560, 569
LweI GCATC 3 cut(s) 165, 535, 694
MaeI CTAG 3 cut(s) 147, 402, 470
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 3 cut(s) 220, 344, 538
MalI GATC 2 cut(s) 264, 442
MboI GATC 2 cut(s) 262, 440
MboII GAAGA 1 cut(s) 202
MfeI CAATTG 1 cut(s) 608
MflI RGATCY 2 cut(s) 262, 440
MhlI GDGCHC 2 cut(s) 193, 339
MluCI AATT 2 cut(s) 57, 608
MlyI GAGTC 1 cut(s) 375
MmeI TCCRAC 2 cut(s) 68, 403
MnlI CCTC 6 cut(s) 122, 211, 281, 325, 611, 660
MroXI GAANNNNTTC 1 cut(s) 471
MseI TTAA 4 cut(s) 15, 63, 459, 582
MslI CAYNNNNRTG 1 cut(s) 218
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 4 cut(s) 73, 83, 84, 737
MunI CAATTG 1 cut(s) 608
Mva1269I GAATGC 1 cut(s) 783
MvaI CCWGG 2 cut(s) 73, 737
MwoI GCNNNNNNNGC 6 cut(s) 74, 554, 557, 563, 695, 704
NciI CCSGG 2 cut(s) 83, 84
NdeII GATC 2 cut(s) 262, 440
NlaIII CATG 3 cut(s) 55, 217, 572
NmuCI GTSAC 2 cut(s) 220, 538
PagI TCATGA 1 cut(s) 568
PctI GAATGC 1 cut(s) 783
PdmI GAANNNNTTC 1 cut(s) 471
Pfl23II CGTACG 1 cut(s) 394
PflMI CCANNNNNTGG 1 cut(s) 742
PkrI GCNGC 2 cut(s) 550, 559
PleI GAGTC 1 cut(s) 375
PpsI GAGTC 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 71, 735
PspGI CCWGG 2 cut(s) 71, 735
PspLI CGTACG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 262, 440
RsaI GTAC 2 cut(s) 396, 726
RsaNI GTAC 2 cut(s) 395, 725
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 4 cut(s) 15, 63, 459, 582
SatI GCNGC 2 cut(s) 549, 558
Sau3AI GATC 2 cut(s) 262, 440
SchI GAGTC 1 cut(s) 375
ScrFI CCNGG 4 cut(s) 73, 83, 84, 737
SduI GDGCHC 2 cut(s) 193, 339
SfaNI GCATC 3 cut(s) 165, 535, 694
SfcI CTRYAG 1 cut(s) 639
SmaI CCCGGG 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 218
SpeI ACTAGT 1 cut(s) 469
Sse9I AATT 2 cut(s) 57, 608
SspMI CTAG 3 cut(s) 147, 402, 470
StyD4I CCNGG 4 cut(s) 71, 81, 82, 735
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 4 cut(s) 136, 173, 394, 412
TaiI ACGT 1 cut(s) 744
TaqI TCGA 2 cut(s) 527, 702
TasI AATT 2 cut(s) 57, 608
TatI WGTACW 1 cut(s) 724
Tru1I TTAA 4 cut(s) 15, 63, 459, 582
Tru9I TTAA 4 cut(s) 15, 63, 459, 582
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 2 cut(s) 220, 538
TseI GCWGC 2 cut(s) 548, 557
Tsp45I GTSAC 2 cut(s) 220, 538
TspDTI ATGAA 5 cut(s) 16, 202, 296, 565, 615
TspMI CCCGGG 1 cut(s) 82
TspRI CASTG 1 cut(s) 139
Van91I CCANNNNNTGG 1 cut(s) 742
VneI GTGCAC 2 cut(s) 189, 335
XmaI CCCGGG 1 cut(s) 82
XmnI GAANNNNTTC 1 cut(s) 471
XspI CTAG 3 cut(s) 147, 402, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.