RLG00000007776

mitochondrial fission

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009680
Physical Location & Seq
Reverse (-)
20915959 .. 20917419
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007776

Sequence Viewer

Length: 1221 bp
ATGGGAGTAGCAAACCAAGATGCTGTAGTTCCCCGCCGCAAGATCCGACATGTCATCAATTCTTGCAAGGAGGAAGGGATTGAAATTCCAGTCCCAACCAGAGCTAGAGACTTGCCAGAGATTGCTAAGAAGATCGATGCCAAGCTCAGTTGCTGCCTCTCGGAACTTAATTTGCTGCCTGCTAGTATTTCATCGGTTGATGAGGCGGTTGCCACTTTCATGCAACTGGTGGCATTGTCCAAAGAATCTCTCATAAAAATTCTTGTGAGAGGAGAATTTGATGAGTATCAGGATGACAAGAGCATGCACGGCACTGCAAACCTGGCTGAGATGCTCAATCAGTTCAAGGATGAGCGTGGCTGGCTGCATTCCAGCTTTTTCCAACGTGACCTCAAAAGTAACTTCCTACGGTCGGAGATTAACTACTGTATGTTTACTCAGCAGGTGGGAAAAGCCAGTGGTCTTGCAGCGAAACTTGCCACGGATCTTACAGCAGATCTAGCAGGGGTGGAACATCATCATGATTCCTGGTTTCTAGATATGTTCTGGTATTACGTGATCATGCAAGTAACTCGTTCTGTGTTGATGACTTATCTATCGAGATATGTTCATCTTTGTATCCATGTTGAGTCACTTGTTTATAATCTAATGGACAAGATGATTCAGAGGTCGTATCAGTGGACGCATGAGAACTATAATACCGTGAATGTGAATGAGGAAAAAACGGAGGAGTTTTTTTCCGAGTATACTAGACTTATGTCCAATTGCAATGTATTCATCCATGGAGTCCTTGATGATCCGGAAAAGCCCTCTACAATTATTGTAGACGGTATTGGGGAGATTGTGGTTGAAGGTCTTAGGAGTTACTCGCGTGATCTACTATCTGAAGCCTTTAGTGTCAGAATGCAATCAATTGCATTTCGGAAGATACATACAGAAACTTTTATTGAAGGTATGGCTTCTCATTTGCAGTCGAGTGTTGCAAACCTTCTGGACACATACATGGATTTGGAGATGCTCGATATGCAGATCTTGAAGAGGCCACATTGTGCTCATGTATTGCTCGATATGCAGATCTTGAAGAGGCCACATTTTGCTCATGTATTTGAGGATATGCTTGAGAACTATCCTTTGGCACGCAAGGGTCTTGAAAAGAGAATCAATATGCTCAAGAAAGCTAAGGAGATTTGTGAGGGGCTCAGGGATGCTTATGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Dynamin_N

Protein Analysis

407

Amino Acids

46.81

Weight (kDa)

5.69

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_M PF01031 37 - 228 3.4e-09 Dynamin central region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 642
AarI CACCTGC 1 cut(s) 433
Acc36I ACCTGC 1 cut(s) 433
AccI GTMKAC 2 cut(s) 746, 825
AccII CGCG 1 cut(s) 871
AccIII TCCGGA 1 cut(s) 799
AciI CCGC 3 cut(s) 34, 37, 206
AclWI GGATC 3 cut(s) 37, 492, 791
AcsI RAATTY 3 cut(s) 84, 258, 275
AcuI CTGAAG 1 cut(s) 906
AdeI CACNNNGTG 1 cut(s) 1049
AfiI CCNNNNNNNGG 1 cut(s) 412
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 7 cut(s) 83, 346, 851, 950, 1036, 1081, 1151
AjnI CCWGG 2 cut(s) 321, 527
AjuI GAANNNNNNNTTGG 2 cut(s) 1115, 1147
AluBI AGCT 4 cut(s) 104, 145, 375, 1178
AluI AGCT 4 cut(s) 104, 145, 375, 1178
Alw21I GWGCWC 1 cut(s) 1054
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 3 cut(s) 37, 492, 791
AlwNI CAGNNNCTG 1 cut(s) 153
Aor13HI TCCGGA 1 cut(s) 799
AoxI GGCC 2 cut(s) 1040, 1085
ApeKI GCWGC 4 cut(s) 153, 175, 364, 467
ApoI RAATTY 3 cut(s) 84, 258, 275
BanII GRGCYC 1 cut(s) 1200
Bbv12I GWGCWC 1 cut(s) 1054
BbvI GCAGC 4 cut(s) 140, 162, 351, 479
BceAI ACGGC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BciT130I CCWGG 2 cut(s) 323, 529
BciVI GTATCC 1 cut(s) 629
BclI TGATCA 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 102
BfaI CTAG 5 cut(s) 105, 183, 500, 536, 750
BfmI CTRYAG 1 cut(s) 24
BfuAI ACCTGC 1 cut(s) 433
BfuI GTATCC 1 cut(s) 629
BglII AGATCT 3 cut(s) 496, 1029, 1074
BisI GCNGC 5 cut(s) 37, 154, 176, 365, 468
BlsI GCNGC 5 cut(s) 38, 155, 177, 366, 469
Bme1390I CCNGG 2 cut(s) 323, 529
BmrFI CCNGG 2 cut(s) 323, 529
BmsI GCATC 5 cut(s) 10, 127, 321, 1005, 1195
BoxI GACNNNNGTC 1 cut(s) 757
Bpu10I CCTNAGC 2 cut(s) 1179, 1199
BpuEI CTTGAG 2 cut(s) 1139, 1154
Bsa29I ATCGAT 1 cut(s) 135
BsaAI YACGTR 1 cut(s) 556
BsaBI GATNNNNATC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 480, 781
BsaWI WCCGGW 1 cut(s) 799
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse1I ACTGG 3 cut(s) 89, 231, 456
Bse3DI GCAATG 1 cut(s) 775
Bse8I GATNNNNATC 1 cut(s) 285
BseAI TCCGGA 1 cut(s) 799
BseBI CCWGG 2 cut(s) 323, 529
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 480, 781
BseGI GGATG 4 cut(s) 298, 355, 777, 1210
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 4 cut(s) 160, 318, 452, 1213
BseNI ACTGG 3 cut(s) 89, 231, 456
BseRI GAGGAG 2 cut(s) 285, 743
BseXI GCAGC 4 cut(s) 140, 162, 351, 479
Bsh1236I CGCG 1 cut(s) 871
Bsh1285I CGRYCG 1 cut(s) 413
BshFI GGCC 2 cut(s) 1042, 1087
BshVI ATCGAT 1 cut(s) 135
BsiEI CGRYCG 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 1054
BsiSI CCGG 1 cut(s) 800
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 2 cut(s) 367, 909
BsnI GGCC 2 cut(s) 1042, 1087
Bsp1286I GDGCHC 2 cut(s) 1054, 1200
Bsp13I TCCGGA 1 cut(s) 799
Bsp143I GATC 9 cut(s) 42, 132, 484, 496, 558, 796, 874, 1029, 1074
Bsp19I CCATGG 1 cut(s) 781
BspACI CCGC 3 cut(s) 34, 37, 206
BspANI GGCC 2 cut(s) 1042, 1087
BspCNI CTCAG 4 cut(s) 159, 319, 451, 1212
BspDI ATCGAT 1 cut(s) 135
BspEI TCCGGA 1 cut(s) 799
BspFNI CGCG 1 cut(s) 871
BspHI TCATGA 1 cut(s) 520
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 3 cut(s) 37, 492, 791
BsrDI GCAATG 1 cut(s) 775
BsrI ACTGG 3 cut(s) 89, 231, 456
BssECI CCNNGG 2 cut(s) 480, 781
BssMI GATC 9 cut(s) 42, 132, 484, 496, 558, 796, 874, 1029, 1074
BssNAI GTATAC 1 cut(s) 747
BssT1I CCWWGG 1 cut(s) 781
Bst1107I GTATAC 1 cut(s) 747
Bst2UI CCWGG 2 cut(s) 323, 529
Bst4CI ACNGT 4 cut(s) 411, 428, 703, 830
Bst6I CTCTTC 2 cut(s) 1031, 1076
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 4 cut(s) 180, 305, 362, 1138
BstDEI CTNAG 7 cut(s) 126, 146, 327, 438, 857, 1179, 1199
BstDSI CCRYGG 2 cut(s) 480, 781
BstF5I GGATG 4 cut(s) 298, 355, 777, 1210
BstFNI CGCG 1 cut(s) 871
BstKTI GATC 9 cut(s) 45, 135, 487, 499, 561, 799, 877, 1032, 1077
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 9 cut(s) 42, 132, 484, 496, 558, 796, 874, 1029, 1074
BstMCI CGRYCG 1 cut(s) 413
BstMWI GCNNNNNNNGC 7 cut(s) 309, 323, 361, 476, 500, 1024, 1069
BstNI CCWGG 2 cut(s) 323, 529
BstNSI RCATGY 2 cut(s) 53, 307
BstPAI GACNNNNGTC 1 cut(s) 757
BstSCI CCNGG 2 cut(s) 321, 527
BstSFI CTRYAG 1 cut(s) 24
BstUI CGCG 1 cut(s) 871
BstV1I GCAGC 4 cut(s) 140, 162, 351, 479
BstX2I RGATCY 5 cut(s) 42, 484, 496, 1029, 1074
BstYI RGATCY 5 cut(s) 42, 484, 496, 1029, 1074
BstZ17I GTATAC 1 cut(s) 747
Bsu15I ATCGAT 1 cut(s) 135
BsuI GTATCC 1 cut(s) 629
BsuRI GGCC 2 cut(s) 1042, 1087
BsuTUI ATCGAT 1 cut(s) 135
BtgI CCRYGG 2 cut(s) 480, 781
BtsCI GGATG 4 cut(s) 298, 355, 777, 1210
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 3 cut(s) 312, 463, 683
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 4 cut(s) 180, 305, 362, 1138
CaiI CAGNNNCTG 1 cut(s) 153
CciI TCATGA 1 cut(s) 520
ClaI ATCGAT 1 cut(s) 135
CseI GACGC 1 cut(s) 691
DdeI CTNAG 7 cut(s) 126, 146, 327, 438, 857, 1179, 1199
DpnI GATC 9 cut(s) 44, 134, 486, 498, 560, 798, 876, 1031, 1076
DpnII GATC 9 cut(s) 42, 132, 484, 496, 558, 796, 874, 1029, 1074
DraIII CACNNNGTG 1 cut(s) 1049
Eam1104I CTCTTC 2 cut(s) 1031, 1076
EarI CTCTTC 2 cut(s) 1031, 1076
Eco130I CCWWGG 1 cut(s) 781
Eco24I GRGCYC 1 cut(s) 1200
Eco57I CTGAAG 1 cut(s) 906
EcoRII CCWGG 2 cut(s) 321, 527
EcoT14I CCWWGG 1 cut(s) 781
EcoT38I GRGCYC 1 cut(s) 1200
ErhI CCWWGG 1 cut(s) 781
FaqI GGGAC 1 cut(s) 77
FauI CCCGC 1 cut(s) 41
FbaI TGATCA 1 cut(s) 558
FblI GTMKAC 2 cut(s) 746, 825
Fnu4HI GCNGC 5 cut(s) 37, 154, 176, 365, 468
FokI GGATG 4 cut(s) 305, 362, 764, 1217
FriOI GRGCYC 1 cut(s) 1200
Fsp4HI GCNGC 5 cut(s) 37, 154, 176, 365, 468
FspBI CTAG 5 cut(s) 105, 183, 500, 536, 750
GluI GCNGC 5 cut(s) 37, 154, 176, 365, 468
HaeIII GGCC 2 cut(s) 1042, 1087
HapII CCGG 1 cut(s) 800
HgaI GACGC 1 cut(s) 691
HinfI GANTC 6 cut(s) 245, 524, 629, 661, 786, 1158
HpaII CCGG 1 cut(s) 800
Hpy166II GTNNAC 4 cut(s) 435, 681, 747, 826
Hpy188I TCNGA 8 cut(s) 47, 163, 415, 666, 742, 886, 902, 924
Hpy8I GTNNAC 4 cut(s) 435, 681, 747, 826
HpyAV CCTTC 4 cut(s) 68, 845, 944, 998
HpyCH4III ACNGT 4 cut(s) 411, 428, 703, 830
HpyCH4IV ACGT 2 cut(s) 385, 555
HpyF10VI GCNNNNNNNGC 7 cut(s) 309, 323, 361, 476, 500, 1024, 1069
HpyF3I CTNAG 7 cut(s) 126, 146, 327, 438, 857, 1179, 1199
HpySE526I ACGT 2 cut(s) 385, 555
Kpn2I TCCGGA 1 cut(s) 799
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 9 cut(s) 42, 132, 484, 496, 558, 796, 874, 1029, 1074
Lsp1109I GCAGC 4 cut(s) 140, 162, 351, 479
LweI GCATC 5 cut(s) 10, 127, 321, 1005, 1195
MaeI CTAG 5 cut(s) 105, 183, 500, 536, 750
MaeII ACGT 2 cut(s) 385, 555
MaeIII GTNAC 5 cut(s) 386, 398, 568, 630, 863
MalI GATC 9 cut(s) 44, 134, 486, 498, 560, 798, 876, 1031, 1076
MboI GATC 9 cut(s) 42, 132, 484, 496, 558, 796, 874, 1029, 1074
MboII GAAGA 4 cut(s) 142, 937, 1048, 1093
MfeI CAATTG 2 cut(s) 763, 912
MflI RGATCY 5 cut(s) 42, 484, 496, 1029, 1074
MhlI GDGCHC 2 cut(s) 1054, 1200
MluCI AATT 8 cut(s) 58, 84, 169, 258, 275, 763, 816, 912
MlyI GAGTC 2 cut(s) 638, 795
MmeI TCCRAC 3 cut(s) 70, 393, 406
MroI TCCGGA 1 cut(s) 799
MseI TTAA 2 cut(s) 168, 420
MslI CAYNNNNRTG 3 cut(s) 218, 519, 1001
MspI CCGG 1 cut(s) 800
MspR9I CCNGG 2 cut(s) 323, 529
MunI CAATTG 2 cut(s) 763, 912
Mva1269I GAATGC 2 cut(s) 367, 909
MvaI CCWGG 2 cut(s) 323, 529
MvnI CGCG 1 cut(s) 871
MwoI GCNNNNNNNGC 7 cut(s) 309, 323, 361, 476, 500, 1024, 1069
NcoI CCATGG 1 cut(s) 781
NdeII GATC 9 cut(s) 42, 132, 484, 496, 558, 796, 874, 1029, 1074
NmuCI GTSAC 2 cut(s) 386, 630
NspI RCATGY 2 cut(s) 53, 307
PaeI GCATGC 1 cut(s) 307
PagI TCATGA 1 cut(s) 520
PaqCI CACCTGC 1 cut(s) 433
PciI ACATGT 1 cut(s) 49
PctI GAATGC 2 cut(s) 367, 909
PfeI GAWTC 4 cut(s) 245, 524, 661, 1158
PkrI GCNGC 5 cut(s) 38, 155, 177, 366, 469
PleI GAGTC 2 cut(s) 637, 794
PpsI GAGTC 2 cut(s) 637, 794
Ppu21I YACGTR 1 cut(s) 556
PscI ACATGT 1 cut(s) 49
PshAI GACNNNNGTC 1 cut(s) 757
PsiI TTATAA 1 cut(s) 642
Psp6I CCWGG 2 cut(s) 321, 527
PspGI CCWGG 2 cut(s) 321, 527
PstNI CAGNNNCTG 1 cut(s) 153
PsuI RGATCY 5 cut(s) 42, 484, 496, 1029, 1074
RseI CAYNNNNRTG 3 cut(s) 218, 519, 1001
SaqAI TTAA 2 cut(s) 168, 420
SatI GCNGC 5 cut(s) 37, 154, 176, 365, 468
Sau3AI GATC 9 cut(s) 42, 132, 484, 496, 558, 796, 874, 1029, 1074
SchI GAGTC 2 cut(s) 638, 795
ScrFI CCNGG 2 cut(s) 323, 529
SduI GDGCHC 2 cut(s) 1054, 1200
SfaNI GCATC 5 cut(s) 10, 127, 321, 1005, 1195
SfcI CTRYAG 1 cut(s) 24
SmiMI CAYNNNNRTG 3 cut(s) 218, 519, 1001
SmlI CTYRAG 2 cut(s) 1118, 1169
SmoI CTYRAG 2 cut(s) 1118, 1169
SphI GCATGC 1 cut(s) 307
Sse9I AATT 8 cut(s) 58, 84, 169, 258, 275, 763, 816, 912
SsiI CCGC 3 cut(s) 34, 37, 206
SspMI CTAG 5 cut(s) 105, 183, 500, 536, 750
StyD4I CCNGG 2 cut(s) 321, 527
StyI CCWWGG 1 cut(s) 781
TaaI ACNGT 4 cut(s) 411, 428, 703, 830
TaiI ACGT 2 cut(s) 388, 558
TaqI TCGA 5 cut(s) 135, 599, 974, 1020, 1065
TasI AATT 8 cut(s) 58, 84, 169, 258, 275, 763, 816, 912
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 4 cut(s) 245, 524, 661, 1158
Tru1I TTAA 2 cut(s) 168, 420
Tru9I TTAA 2 cut(s) 168, 420
TscAI CASTG 3 cut(s) 319, 463, 683
TseFI GTSAC 2 cut(s) 386, 630
TseI GCWGC 4 cut(s) 153, 175, 364, 467
Tsp45I GTSAC 2 cut(s) 386, 630
TspDTI ATGAA 4 cut(s) 180, 208, 599, 766
TspGWI ACGGA 2 cut(s) 497, 740
TspRI CASTG 3 cut(s) 319, 463, 683
XapI RAATTY 3 cut(s) 84, 258, 275
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 2 cut(s) 53, 307
XmiI GTMKAC 2 cut(s) 746, 825
XspI CTAG 5 cut(s) 105, 183, 500, 536, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.