Rh4AG224200

mitochondrial fission

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009680
Physical Location & Seq
Forward (+)
52412371 .. 52425928
13558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG224200.1

Sequence Viewer

Length: 777 bp
ATGGGGAGAAAGAGAAAAAACAAATCATCAAGAGGTTCCAAAGAAAGAAAGACCAGATCAGAAATGGGAGTAGCAAACCAAGATGCTGTAGTTCCTCGCCGCAAGATCCGACATGTCATCAATTCTTGCAAGGAGGAAGGGATTGAAATTCCAGTCCCAACCAGAGCTAGAGACTTGCCAGAGATTGCTAAGAAGATCGATGCCAAGCTCAGTTACTGCCTCTCGGAACTTAATTTGCTGCCTGCTAGTATTTCATCGGTTGATGAGGCGGTTGCCACTTCCATGCAACTGGTGGCATTGTCCAAAGAATCTCTCATAAAAATTCTTGTGAGAGGAGAATTTGATGAGTATCCTGATGACAAGAGCATGCACGGCACTGCAAACCTGGCTGAGATGCTCAATCAGTTCAAGGATGAACGTGGCTGGCTGCATTCCAGCTTTTTCCGACGTGACCTCAAAAGTAACTTCCTACAGTCGGAGATCAACTACTGTATGTTTACTCAGCAGGTGGGAAAAGCCAGTGGTCTTGCAGAGAAACTTGCCGCGGATCTTACAGCAGATCTAGTCGCGGTGGAACATCACCATGATTCCTGGTTTCTGGATATGTTCTGGTATTACGTGTTCATGCAACTATACAAAGGACTTGGCTGGATGAGGCTCAACTTGACTAAAAATCGGTTTGCCCGAAGAGCATCGAGGGGTACTTTTGTGAGAATCAAGCCGATCAGTAGAGGAAACGAGGGAGTAGTAAGGAAAGTAGGAGGTGGGATTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Dynamin_N

Protein Analysis

258

Amino Acids

29.34

Weight (kDa)

9.72

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_M PF01031 58 - 165 1.8e-07 Dynamin central region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 496
Acc36I ACCTGC 1 cut(s) 496
AccII CGCG 2 cut(s) 545, 569
AciI CCGC 5 cut(s) 100, 269, 543, 545, 569
AclWI GGATC 2 cut(s) 100, 555
AcsI RAATTY 3 cut(s) 147, 321, 338
AfaI GTAC 1 cut(s) 703
AfiI CCNNNNNNNGG 1 cut(s) 475
AflIII ACRYGT 2 cut(s) 112, 618
AgsI TTSAA 2 cut(s) 146, 409
AjiI CACGTC 1 cut(s) 449
AjnI CCWGG 2 cut(s) 384, 590
AluBI AGCT 3 cut(s) 167, 208, 438
AluI AGCT 3 cut(s) 167, 208, 438
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 2 cut(s) 100, 555
AlwNI CAGNNNCTG 1 cut(s) 216
ApeKI GCWGC 2 cut(s) 238, 427
ApoI RAATTY 3 cut(s) 147, 321, 338
AsuHPI GGTGA 1 cut(s) 572
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BbvI GCAGC 2 cut(s) 225, 414
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 2 cut(s) 386, 592
BciVI GTATCC 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 3 cut(s) 168, 246, 563
BfmI CTRYAG 2 cut(s) 87, 470
BfuAI ACCTGC 1 cut(s) 496
BfuI GTATCC 1 cut(s) 360
BglII AGATCT 1 cut(s) 559
BisI GCNGC 4 cut(s) 100, 239, 428, 543
BlsI GCNGC 4 cut(s) 101, 240, 429, 544
Bme1390I CCNGG 2 cut(s) 386, 592
BmgBI CACGTC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 2 cut(s) 386, 592
BmsI GCATC 4 cut(s) 73, 190, 384, 701
Bsa29I ATCGAT 1 cut(s) 198
BsaAI YACGTR 1 cut(s) 619
BsaBI GATNNNNATC 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 543
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse1I ACTGG 3 cut(s) 152, 294, 519
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 2 cut(s) 386, 592
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 543
BseGI GGATG 2 cut(s) 418, 657
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMII CTCAG 3 cut(s) 223, 381, 515
BseNI ACTGG 3 cut(s) 152, 294, 519
BseRI GAGGAG 1 cut(s) 348
BseXI GCAGC 2 cut(s) 225, 414
Bsh1236I CGCG 2 cut(s) 545, 569
BshVI ATCGAT 1 cut(s) 198
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 430
Bsp143I GATC 7 cut(s) 56, 105, 195, 480, 547, 559, 723
BspACI CCGC 5 cut(s) 100, 269, 543, 545, 569
BspCNI CTCAG 3 cut(s) 222, 382, 514
BspDI ATCGAT 1 cut(s) 198
BspFNI CGCG 2 cut(s) 545, 569
BspLI GGNNCC 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 496
BspPI GGATC 2 cut(s) 100, 555
BspQI GCTCTTC 1 cut(s) 682
BsrI ACTGG 3 cut(s) 152, 294, 519
BssECI CCNNGG 1 cut(s) 543
BssMI GATC 7 cut(s) 56, 105, 195, 480, 547, 559, 723
Bst2UI CCWGG 2 cut(s) 386, 592
Bst4CI ACNGT 2 cut(s) 474, 491
Bst6I CTCTTC 1 cut(s) 682
BstBAI YACGTR 1 cut(s) 619
BstC8I GCNNGC 3 cut(s) 243, 368, 425
BstDEI CTNAG 4 cut(s) 189, 209, 390, 501
BstDSI CCRYGG 1 cut(s) 543
BstF5I GGATG 2 cut(s) 418, 657
BstFNI CGCG 2 cut(s) 545, 569
BstKTI GATC 7 cut(s) 59, 108, 198, 483, 550, 562, 726
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 7 cut(s) 56, 105, 195, 480, 547, 559, 723
BstMWI GCNNNNNNNGC 3 cut(s) 372, 386, 689
BstNI CCWGG 2 cut(s) 386, 592
BstNSI RCATGY 2 cut(s) 116, 370
BstSCI CCNGG 2 cut(s) 384, 590
BstSFI CTRYAG 2 cut(s) 87, 470
BstUI CGCG 2 cut(s) 545, 569
BstV1I GCAGC 2 cut(s) 225, 414
BstX2I RGATCY 3 cut(s) 105, 547, 559
BstXI CCANNNNNNTGG 1 cut(s) 289
BstYI RGATCY 3 cut(s) 105, 547, 559
Bsu15I ATCGAT 1 cut(s) 198
BsuI GTATCC 1 cut(s) 360
BsuTUI ATCGAT 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 543
BtrI CACGTC 1 cut(s) 449
BtsCI GGATG 2 cut(s) 418, 657
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 375, 526
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 3 cut(s) 243, 368, 425
CaiI CAGNNNCTG 1 cut(s) 216
Cfr42I CCGCGG 1 cut(s) 546
ClaI ATCGAT 1 cut(s) 198
Csp6I GTAC 1 cut(s) 702
CviAII CATG 6 cut(s) 113, 283, 367, 584, 625, 774
CviQI GTAC 1 cut(s) 702
DdeI CTNAG 4 cut(s) 189, 209, 390, 501
DpnI GATC 7 cut(s) 58, 107, 197, 482, 549, 561, 725
DpnII GATC 7 cut(s) 56, 105, 195, 480, 547, 559, 723
Eam1104I CTCTTC 1 cut(s) 682
EarI CTCTTC 1 cut(s) 682
EcoRII CCWGG 2 cut(s) 384, 590
FaeI CATG 6 cut(s) 116, 286, 370, 587, 628, 777
FaqI GGGAC 1 cut(s) 140
FatI CATG 6 cut(s) 112, 282, 366, 583, 624, 773
Fnu4HI GCNGC 4 cut(s) 100, 239, 428, 543
FokI GGATG 2 cut(s) 425, 664
Fsp4HI GCNGC 4 cut(s) 100, 239, 428, 543
FspBI CTAG 3 cut(s) 168, 246, 563
GluI GCNGC 4 cut(s) 100, 239, 428, 543
Hin1II CATG 6 cut(s) 116, 286, 370, 587, 628, 777
HinfI GANTC 4 cut(s) 308, 587, 714, 769
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 1 cut(s) 498
Hpy188I TCNGA 5 cut(s) 61, 110, 226, 446, 478
Hpy188III TCNNGA 3 cut(s) 30, 353, 599
Hpy8I GTNNAC 1 cut(s) 498
Hpy99I CGWCG 1 cut(s) 450
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 2 cut(s) 474, 491
HpyCH4IV ACGT 3 cut(s) 418, 448, 618
HpyCH4V TGCA 7 cut(s) 129, 286, 370, 380, 430, 530, 628
HpyF10VI GCNNNNNNNGC 3 cut(s) 372, 386, 689
HpyF3I CTNAG 4 cut(s) 189, 209, 390, 501
HpySE526I ACGT 3 cut(s) 418, 448, 618
Hsp92II CATG 6 cut(s) 116, 286, 370, 587, 628, 777
KspI CCGCGG 1 cut(s) 546
Kzo9I GATC 7 cut(s) 56, 105, 195, 480, 547, 559, 723
LguI GCTCTTC 1 cut(s) 682
Lsp1109I GCAGC 2 cut(s) 225, 414
LweI GCATC 4 cut(s) 73, 190, 384, 701
MaeI CTAG 3 cut(s) 168, 246, 563
MaeII ACGT 3 cut(s) 418, 448, 618
MaeIII GTNAC 3 cut(s) 212, 449, 461
MalI GATC 7 cut(s) 58, 107, 197, 482, 549, 561, 725
MboI GATC 7 cut(s) 56, 105, 195, 480, 547, 559, 723
MboII GAAGA 2 cut(s) 205, 699
MflI RGATCY 3 cut(s) 105, 547, 559
MluCI AATT 5 cut(s) 121, 147, 232, 321, 338
MmeI TCCRAC 3 cut(s) 133, 456, 469
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 2 cut(s) 281, 582
MspA1I CMGCKG 1 cut(s) 545
MspR9I CCNGG 2 cut(s) 386, 592
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 2 cut(s) 386, 592
MvnI CGCG 2 cut(s) 545, 569
MwoI GCNNNNNNNGC 3 cut(s) 372, 386, 689
NdeII GATC 7 cut(s) 56, 105, 195, 480, 547, 559, 723
NlaIII CATG 6 cut(s) 116, 286, 370, 587, 628, 777
NlaIV GGNNCC 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 449
NspI RCATGY 2 cut(s) 116, 370
PaeI GCATGC 1 cut(s) 370
PaqCI CACCTGC 1 cut(s) 496
PciI ACATGT 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 682
PcsI WCGNNNNNNNCGW 1 cut(s) 682
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 4 cut(s) 308, 587, 714, 769
PkrI GCNGC 4 cut(s) 101, 240, 429, 544
Ppu21I YACGTR 1 cut(s) 619
PscI ACATGT 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 384, 590
PspGI CCWGG 2 cut(s) 384, 590
PspN4I GGNNCC 1 cut(s) 37
PsrI GAACNNNNNNTAC 2 cut(s) 605, 637
PstNI CAGNNNCTG 1 cut(s) 216
PsuI RGATCY 3 cut(s) 105, 547, 559
RsaI GTAC 1 cut(s) 703
RsaNI GTAC 1 cut(s) 702
RseI CAYNNNNRTG 2 cut(s) 281, 582
SacII CCGCGG 1 cut(s) 546
SapI GCTCTTC 1 cut(s) 682
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 4 cut(s) 100, 239, 428, 543
Sau3AI GATC 7 cut(s) 56, 105, 195, 480, 547, 559, 723
ScrFI CCNGG 2 cut(s) 386, 592
SfaNI GCATC 4 cut(s) 73, 190, 384, 701
SfcI CTRYAG 2 cut(s) 87, 470
Sfr303I CCGCGG 1 cut(s) 546
SgrBI CCGCGG 1 cut(s) 546
SmiMI CAYNNNNRTG 2 cut(s) 281, 582
SphI GCATGC 1 cut(s) 370
Sse9I AATT 5 cut(s) 121, 147, 232, 321, 338
SsiI CCGC 5 cut(s) 100, 269, 543, 545, 569
SspMI CTAG 3 cut(s) 168, 246, 563
StyD4I CCNGG 2 cut(s) 384, 590
TaaI ACNGT 2 cut(s) 474, 491
TaiI ACGT 3 cut(s) 421, 451, 621
TaqI TCGA 2 cut(s) 198, 695
TasI AATT 5 cut(s) 121, 147, 232, 321, 338
TauI GCSGC 2 cut(s) 102, 545
TfiI GAWTC 4 cut(s) 308, 587, 714, 769
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 2 cut(s) 382, 526
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 2 cut(s) 238, 427
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 3 cut(s) 243, 429, 613
TspRI CASTG 2 cut(s) 382, 526
XapI RAATTY 3 cut(s) 147, 321, 338
XceI RCATGY 2 cut(s) 116, 370
XcmI CCANNNNNNNNNTGG 2 cut(s) 61, 289
XspI CTAG 3 cut(s) 168, 246, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.