Rh4DG222800

mitochondrial fission

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009680
Physical Location & Seq
Forward (+)
42585994 .. 42592935
6942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG222800.1

Sequence Viewer

Length: 834 bp
ATGGGGAGAAAGAGAAAAAACAAATCATCAAGAGGTTCCAAAGAAAGAAAGACCAGATCAGAAGTGGGAGTAGCAAACCAAGATGCTGTAGTTCTTCGCCGCAAGATCCAACATGTCATCAATTCTTGCAAGGAAGAAGGGATTGAAATGCCAGTCCCAACCAGAGCTAGAGACTTGCCAGAGATTGCTAAGAAGATCGATGCCAAGCTCAGTTACTGCCTCTCGGAACTTAATTTGCTGCCTGCTAGTATTTCATCGGTTGATGAGGCGGTTGCCACTTTCATGCAACTGGTGGCATTGTCCAAAGAATCTCTCATAAAAATTCTTGTGAGAGGAGAATTTGATGAGTATCCTGATGACAAGAGCATGCACGGCACTGCAAACCTGGCTGGGATGCTCAATCAGTTCAAGGATGAACGTGGCTGGCTGCATTCCAGCTTTTTCCGACGTGACCTCAAAAGTAACTTCCTACAGTCGGAGATCAACTACTGTATGTTTACTCAGCAGGTGGGAAAAGCCAGTGGTCTTGCAGAGAAACTTGCCGCGGATCTTACAGCAGATCTAGTCGCGGTGGAACATCACCATGATTCCTGGTTTCTGGATATGTTCTGGTATTACGTGATCATGCAATCGAGTGTTGCAAACCTTCTGGACACATACATGGATTTGGAGATGTTCGATATGCAGATCTTGAAGAGGCCACATTTTGCTCATGTATTTGAGGATATGGTTGAGAACTATCCCTTGGCACGCAAGGGTCTTGAAAAGAGAATCAATATGCTCAAGAAAGCTAAGGAGATTTGTGAGGGGATCATGGATGCTTATGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Dynamin_N

Protein Analysis

277

Amino Acids

31.78

Weight (kDa)

6.47

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_M PF01031 58 - 165 6.2e-08 Dynamin central region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 496
Acc36I ACCTGC 1 cut(s) 496
AccII CGCG 2 cut(s) 545, 569
AciI CCGC 5 cut(s) 100, 269, 543, 545, 569
AclWI GGATC 3 cut(s) 100, 555, 818
AcsI RAATTY 2 cut(s) 321, 338
AfiI CCNNNNNNNGG 1 cut(s) 475
AflIII ACRYGT 1 cut(s) 112
AgsI TTSAA 4 cut(s) 146, 409, 694, 764
AjiI CACGTC 1 cut(s) 449
AjnI CCWGG 2 cut(s) 384, 590
AjuI GAANNNNNNNTTGG 2 cut(s) 728, 760
AluBI AGCT 4 cut(s) 167, 208, 438, 791
AluI AGCT 4 cut(s) 167, 208, 438, 791
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 3 cut(s) 100, 555, 818
AlwNI CAGNNNCTG 1 cut(s) 216
AoxI GGCC 1 cut(s) 698
ApeKI GCWGC 2 cut(s) 238, 427
ApoI RAATTY 2 cut(s) 321, 338
AsuHPI GGTGA 1 cut(s) 572
BbvI GCAGC 2 cut(s) 225, 414
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 2 cut(s) 386, 592
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 1 cut(s) 621
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 3 cut(s) 168, 246, 563
BfmI CTRYAG 2 cut(s) 87, 470
BfuAI ACCTGC 1 cut(s) 496
BfuI GTATCC 1 cut(s) 360
BglII AGATCT 2 cut(s) 559, 687
BisI GCNGC 4 cut(s) 100, 239, 428, 543
BlsI GCNGC 4 cut(s) 101, 240, 429, 544
Bme1390I CCNGG 2 cut(s) 386, 592
BmgBI CACGTC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 2 cut(s) 386, 592
BmsI GCATC 4 cut(s) 73, 190, 384, 808
Bpu10I CCTNAGC 1 cut(s) 792
BpuEI CTTGAG 1 cut(s) 767
Bsa29I ATCGAT 1 cut(s) 198
BsaAI YACGTR 1 cut(s) 619
BsaBI GATNNNNATC 1 cut(s) 348
BsaJI CCNNGG 2 cut(s) 543, 744
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse1I ACTGG 3 cut(s) 152, 294, 519
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 2 cut(s) 386, 592
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 2 cut(s) 543, 744
BseGI GGATG 3 cut(s) 399, 418, 823
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMII CTCAG 2 cut(s) 223, 515
BseNI ACTGG 3 cut(s) 152, 294, 519
BseRI GAGGAG 1 cut(s) 348
BseXI GCAGC 2 cut(s) 225, 414
BseYI CCCAGC 1 cut(s) 389
Bsh1236I CGCG 2 cut(s) 545, 569
BshFI GGCC 1 cut(s) 700
BshVI ATCGAT 1 cut(s) 198
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 1 cut(s) 700
Bsp143I GATC 9 cut(s) 56, 105, 195, 480, 547, 559, 621, 687, 810
BspACI CCGC 5 cut(s) 100, 269, 543, 545, 569
BspANI GGCC 1 cut(s) 700
BspCNI CTCAG 2 cut(s) 222, 514
BspDI ATCGAT 1 cut(s) 198
BspFNI CGCG 2 cut(s) 545, 569
BspLI GGNNCC 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 496
BspPI GGATC 3 cut(s) 100, 555, 818
BsrI ACTGG 3 cut(s) 152, 294, 519
BssECI CCNNGG 2 cut(s) 543, 744
BssMI GATC 9 cut(s) 56, 105, 195, 480, 547, 559, 621, 687, 810
BssT1I CCWWGG 1 cut(s) 744
Bst2UI CCWGG 2 cut(s) 386, 592
Bst4CI ACNGT 2 cut(s) 474, 491
Bst6I CTCTTC 1 cut(s) 689
BstBAI YACGTR 1 cut(s) 619
BstC8I GCNNGC 4 cut(s) 243, 368, 425, 751
BstDEI CTNAG 4 cut(s) 189, 209, 501, 792
BstDSI CCRYGG 1 cut(s) 543
BstF5I GGATG 3 cut(s) 399, 418, 823
BstFNI CGCG 2 cut(s) 545, 569
BstKTI GATC 9 cut(s) 59, 108, 198, 483, 550, 562, 624, 690, 813
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 9 cut(s) 56, 105, 195, 480, 547, 559, 621, 687, 810
BstMWI GCNNNNNNNGC 2 cut(s) 372, 386
BstNI CCWGG 2 cut(s) 386, 592
BstNSI RCATGY 2 cut(s) 116, 370
BstSCI CCNGG 2 cut(s) 384, 590
BstSFI CTRYAG 2 cut(s) 87, 470
BstUI CGCG 2 cut(s) 545, 569
BstV1I GCAGC 2 cut(s) 225, 414
BstX2I RGATCY 4 cut(s) 105, 547, 559, 687
BstYI RGATCY 4 cut(s) 105, 547, 559, 687
Bsu15I ATCGAT 1 cut(s) 198
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 1 cut(s) 700
BsuTUI ATCGAT 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 543
BtrI CACGTC 1 cut(s) 449
BtsCI GGATG 3 cut(s) 399, 418, 823
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 375, 526
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 4 cut(s) 243, 368, 425, 751
CaiI CAGNNNCTG 1 cut(s) 216
Cfr42I CCGCGG 1 cut(s) 546
ClaI ATCGAT 1 cut(s) 198
CviAII CATG 8 cut(s) 113, 283, 367, 584, 625, 661, 713, 814
CviJI RGCY 9 cut(s) 167, 208, 389, 423, 427, 438, 518, 700, 791
CviKI_1 RGCY 9 cut(s) 167, 208, 389, 423, 427, 438, 518, 700, 791
DdeI CTNAG 4 cut(s) 189, 209, 501, 792
DpnI GATC 9 cut(s) 58, 107, 197, 482, 549, 561, 623, 689, 812
DpnII GATC 9 cut(s) 56, 105, 195, 480, 547, 559, 621, 687, 810
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
Eco130I CCWWGG 1 cut(s) 744
EcoRII CCWGG 2 cut(s) 384, 590
EcoT14I CCWWGG 1 cut(s) 744
ErhI CCWWGG 1 cut(s) 744
FaeI CATG 8 cut(s) 116, 286, 370, 587, 628, 664, 716, 817
FaqI GGGAC 1 cut(s) 140
FatI CATG 8 cut(s) 112, 282, 366, 583, 624, 660, 712, 813
FbaI TGATCA 1 cut(s) 621
Fnu4HI GCNGC 4 cut(s) 100, 239, 428, 543
FokI GGATG 3 cut(s) 406, 425, 830
Fsp4HI GCNGC 4 cut(s) 100, 239, 428, 543
FspBI CTAG 3 cut(s) 168, 246, 563
GluI GCNGC 4 cut(s) 100, 239, 428, 543
GsaI CCCAGC 1 cut(s) 393
HaeIII GGCC 1 cut(s) 700
Hin1II CATG 8 cut(s) 116, 286, 370, 587, 628, 664, 716, 817
HinfI GANTC 3 cut(s) 308, 587, 771
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 1 cut(s) 498
Hpy188I TCNGA 4 cut(s) 61, 226, 446, 478
Hpy188III TCNNGA 7 cut(s) 30, 353, 599, 650, 691, 761, 784
Hpy8I GTNNAC 1 cut(s) 498
Hpy99I CGWCG 1 cut(s) 450
HpyAV CCTTC 2 cut(s) 131, 656
HpyCH4III ACNGT 2 cut(s) 474, 491
HpyCH4IV ACGT 3 cut(s) 418, 448, 618
HpyCH4V TGCA 9 cut(s) 129, 286, 370, 380, 430, 530, 628, 641, 685
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 386
HpyF3I CTNAG 4 cut(s) 189, 209, 501, 792
HpySE526I ACGT 3 cut(s) 418, 448, 618
Hsp92II CATG 8 cut(s) 116, 286, 370, 587, 628, 664, 716, 817
Ksp22I TGATCA 1 cut(s) 621
KspI CCGCGG 1 cut(s) 546
Kzo9I GATC 9 cut(s) 56, 105, 195, 480, 547, 559, 621, 687, 810
Lsp1109I GCAGC 2 cut(s) 225, 414
LweI GCATC 4 cut(s) 73, 190, 384, 808
MaeI CTAG 3 cut(s) 168, 246, 563
MaeII ACGT 3 cut(s) 418, 448, 618
MaeIII GTNAC 3 cut(s) 212, 449, 461
MalI GATC 9 cut(s) 58, 107, 197, 482, 549, 561, 623, 689, 812
MboI GATC 9 cut(s) 56, 105, 195, 480, 547, 559, 621, 687, 810
MboII GAAGA 4 cut(s) 86, 146, 205, 706
MflI RGATCY 4 cut(s) 105, 547, 559, 687
MluCI AATT 4 cut(s) 121, 232, 321, 338
MmeI TCCRAC 3 cut(s) 133, 456, 469
MnlI CCTC 8 cut(s) 26, 230, 259, 326, 464, 690, 715, 799
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 3 cut(s) 281, 582, 659
MspA1I CMGCKG 1 cut(s) 545
MspR9I CCNGG 2 cut(s) 386, 592
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 2 cut(s) 386, 592
MvnI CGCG 2 cut(s) 545, 569
MwoI GCNNNNNNNGC 2 cut(s) 372, 386
NdeII GATC 9 cut(s) 56, 105, 195, 480, 547, 559, 621, 687, 810
NlaIII CATG 8 cut(s) 116, 286, 370, 587, 628, 664, 716, 817
NlaIV GGNNCC 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 449
NspI RCATGY 2 cut(s) 116, 370
PaeI GCATGC 1 cut(s) 370
PaqCI CACCTGC 1 cut(s) 496
PciI ACATGT 1 cut(s) 112
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 3 cut(s) 308, 587, 771
PkrI GCNGC 4 cut(s) 101, 240, 429, 544
Ppu21I YACGTR 1 cut(s) 619
PscI ACATGT 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 384, 590
PspFI CCCAGC 1 cut(s) 389
PspGI CCWGG 2 cut(s) 384, 590
PspN4I GGNNCC 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 216
PsuI RGATCY 4 cut(s) 105, 547, 559, 687
RseI CAYNNNNRTG 3 cut(s) 281, 582, 659
SacII CCGCGG 1 cut(s) 546
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 4 cut(s) 100, 239, 428, 543
Sau3AI GATC 9 cut(s) 56, 105, 195, 480, 547, 559, 621, 687, 810
ScrFI CCNGG 2 cut(s) 386, 592
SfaNI GCATC 4 cut(s) 73, 190, 384, 808
SfcI CTRYAG 2 cut(s) 87, 470
Sfr303I CCGCGG 1 cut(s) 546
SgrBI CCGCGG 1 cut(s) 546
SmiMI CAYNNNNRTG 3 cut(s) 281, 582, 659
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
SphI GCATGC 1 cut(s) 370
Sse9I AATT 4 cut(s) 121, 232, 321, 338
SsiI CCGC 5 cut(s) 100, 269, 543, 545, 569
SspMI CTAG 3 cut(s) 168, 246, 563
StyD4I CCNGG 2 cut(s) 384, 590
StyI CCWWGG 1 cut(s) 744
TaaI ACNGT 2 cut(s) 474, 491
TaiI ACGT 3 cut(s) 421, 451, 621
TaqI TCGA 3 cut(s) 198, 632, 678
TasI AATT 4 cut(s) 121, 232, 321, 338
TauI GCSGC 2 cut(s) 102, 545
TfiI GAWTC 3 cut(s) 308, 587, 771
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 2 cut(s) 382, 526
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 2 cut(s) 238, 427
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 3 cut(s) 243, 271, 429
TspRI CASTG 2 cut(s) 382, 526
XapI RAATTY 2 cut(s) 321, 338
XceI RCATGY 2 cut(s) 116, 370
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XspI CTAG 3 cut(s) 168, 246, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.