Rroxscaffold_5G00362950

mitochondrial fission

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009680
Physical Location & Seq
Forward (+)
43987494 .. 43988669
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00362950.1

Sequence Viewer

Length: 1176 bp
ATGGGTGTAGCAAACCAAGATTCTGTAGTTCCTCGCCGCATGATCCGACATGTCATCAATTCTTGCAAAGAGGAAGGGATTGAAATTCCAGTCCCAACCAGAGCTAGAGACTTGCCAGAGATTGCTAAGAAGATCGATGCCAAGCTCAGTTACTGCCTCTCGGAACTTAATTTGCTGCCTGCTAGTATTTCATCGGTTGATGAGGCGGTTGCCACTTTCATGCAACTGGTGGCATTGTCCAAAGAATCTCTCATAAAAATTCTTGTAAGGGGAGAATTTGATGAGTATCCGGATGACAAGAGCATGCGCGGCACTGCAAACCTGGCTGAGATGCTCAATCAATTCAAGGATGAACGTGGCTGGCTGCATTCCAGCTTTTTCCGACGTGACCTCAAAAGTAACTTCCTACAGTCGGAGATCAACTACTGTATGTTTACTCAGCAGGTGGGAAAAGCCAGTGGTCTTGCAGCGAAACTTGCCGCGGATCTTACAGCAGATCTAGTAGCGGTGGAACATCACCATGATTCCTGGTTTCTGGATATGTTCTGGTATTACGTGATCATGCAAGTAACTCGTTCTGTGTTAATGACTTATCTATCGAGATATGTTCATCTTTGTATTCATGCTGTGTCACTTGTTTATAATCTAATGGCCAAGATGATTCAGAGGTCGTATCAGTGGGCGCATGAGAACTATTATACCGTGAATGTGAATGAGGAAAAAACGGATGAGTTTTTTTCCGAGTATACTAGACTTATGTCCAAATGCAATGTATTCATCCATGGAGTCCTTGATGATCCGGAAAAGCCCTCTACAATTATTGTAGACGGTATTGGGGAGATTGTGGTTGAAGGTCTTAGGAGTTACTCGCGTGATCTACTATCTGAAGCCTTTAGCGTCAGAATGCAATCAATTGCATTTCGGAAGATACATACAGAAACTTTTATTGAAGGTATGGCTTCTCATTTGCAGTCGAGTGTTGAAAACCTTCTGGACACATACATGGATTTGGAGATGTGCGATACGCAGATCTTGAAGACGCCACATTTTGCTCATGTATTTGAGGATATGCTTGAGAACTATCCTTTGGCACGCAAGGATCTTGAAAAGAGAATCAATATGCTCAAGAAAGCTAAGGAGATTTGTGAGGGGATCATGGATGATTATGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Dynamin_N

Protein Analysis

391

Amino Acids

45.09

Weight (kDa)

5.32

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_M PF01031 37 - 227 6.9e-09 Dynamin central region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 642
AarI CACCTGC 1 cut(s) 433
Acc36I ACCTGC 1 cut(s) 433
AccI GTMKAC 2 cut(s) 746, 825
AccII CGCG 3 cut(s) 309, 482, 871
AccIII TCCGGA 2 cut(s) 289, 799
AciI CCGC 6 cut(s) 37, 206, 309, 480, 482, 506
AclWI GGATC 5 cut(s) 37, 492, 791, 1107, 1160
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 3 cut(s) 84, 258, 275
AcuI CTGAAG 1 cut(s) 906
AcyI GRCGYC 1 cut(s) 1040
AfiI CCNNNNNNNGG 1 cut(s) 412
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 7 cut(s) 83, 346, 851, 950, 983, 1036, 1106
AjiI CACGTC 1 cut(s) 386
AjnI CCWGG 2 cut(s) 321, 527
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AluBI AGCT 4 cut(s) 104, 145, 375, 1133
AluI AGCT 4 cut(s) 104, 145, 375, 1133
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 5 cut(s) 37, 492, 791, 1107, 1160
AlwNI CAGNNNCTG 1 cut(s) 153
Aor13HI TCCGGA 2 cut(s) 289, 799
AoxI GGCC 1 cut(s) 651
ApeKI GCWGC 3 cut(s) 175, 364, 467
ApoI RAATTY 3 cut(s) 84, 258, 275
Asp700I GAANNNNTTC 1 cut(s) 987
AspLEI GCGC 2 cut(s) 309, 685
AsuHPI GGTGA 1 cut(s) 509
BalI TGGCCA 1 cut(s) 653
BbsI GAAGAC 1 cut(s) 1043
BbvI GCAGC 3 cut(s) 162, 351, 479
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BciT130I CCWGG 2 cut(s) 323, 529
BciVI GTATCC 1 cut(s) 297
BclI TGATCA 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 102
BfaI CTAG 4 cut(s) 105, 183, 500, 750
BfmI CTRYAG 2 cut(s) 24, 407
BfuAI ACCTGC 1 cut(s) 433
BfuI GTATCC 1 cut(s) 297
BglII AGATCT 2 cut(s) 496, 1029
BisI GCNGC 6 cut(s) 37, 176, 310, 365, 468, 480
BlsI GCNGC 6 cut(s) 38, 177, 311, 366, 469, 481
Bme1390I CCNGG 2 cut(s) 323, 529
BmgBI CACGTC 1 cut(s) 386
BmrFI CCNGG 2 cut(s) 323, 529
BmsI GCATC 2 cut(s) 127, 321
BoxI GACNNNNGTC 1 cut(s) 757
BpiI GAAGAC 1 cut(s) 1043
Bpu10I CCTNAGC 1 cut(s) 1134
BpuEI CTTGAG 2 cut(s) 1094, 1109
Bsa29I ATCGAT 1 cut(s) 135
BsaAI YACGTR 1 cut(s) 556
BsaBI GATNNNNATC 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 1040
BsaJI CCNNGG 2 cut(s) 480, 781
BsaWI WCCGGW 2 cut(s) 289, 799
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse1I ACTGG 3 cut(s) 89, 231, 456
Bse3DI GCAATG 1 cut(s) 775
Bse8I GATNNNNATC 1 cut(s) 285
BseAI TCCGGA 2 cut(s) 289, 799
BseBI CCWGG 2 cut(s) 323, 529
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 480, 781
BseGI GGATG 5 cut(s) 298, 355, 733, 777, 1165
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 3 cut(s) 160, 318, 452
BseNI ACTGG 3 cut(s) 89, 231, 456
BseXI GCAGC 3 cut(s) 162, 351, 479
Bsh1236I CGCG 3 cut(s) 309, 482, 871
BshFI GGCC 1 cut(s) 653
BshVI ATCGAT 1 cut(s) 135
BsiSI CCGG 2 cut(s) 290, 800
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 2 cut(s) 367, 909
BsnI GGCC 1 cut(s) 653
Bsp13I TCCGGA 2 cut(s) 289, 799
Bsp19I CCATGG 1 cut(s) 781
BspACI CCGC 6 cut(s) 37, 206, 309, 480, 482, 506
BspANI GGCC 1 cut(s) 653
BspCNI CTCAG 3 cut(s) 159, 319, 451
BspDI ATCGAT 1 cut(s) 135
BspEI TCCGGA 2 cut(s) 289, 799
BspFNI CGCG 3 cut(s) 309, 482, 871
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 5 cut(s) 37, 492, 791, 1107, 1160
BsrDI GCAATG 1 cut(s) 775
BsrI ACTGG 3 cut(s) 89, 231, 456
BssECI CCNNGG 2 cut(s) 480, 781
BssNAI GTATAC 1 cut(s) 747
BssNI GRCGYC 1 cut(s) 1040
BssT1I CCWWGG 1 cut(s) 781
Bst1107I GTATAC 1 cut(s) 747
Bst2UI CCWGG 2 cut(s) 323, 529
Bst4CI ACNGT 4 cut(s) 411, 428, 703, 830
BstACI GRCGYC 1 cut(s) 1040
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 4 cut(s) 180, 305, 362, 1093
BstDEI CTNAG 6 cut(s) 126, 146, 327, 438, 857, 1134
BstDSI CCRYGG 2 cut(s) 480, 781
BstF5I GGATG 5 cut(s) 298, 355, 733, 777, 1165
BstFNI CGCG 3 cut(s) 309, 482, 871
BstHHI GCGC 2 cut(s) 309, 685
BstMAI GTCTC 1 cut(s) 102
BstMWI GCNNNNNNNGC 3 cut(s) 309, 323, 476
BstNI CCWGG 2 cut(s) 323, 529
BstNSI RCATGY 2 cut(s) 53, 307
BstPAI GACNNNNGTC 1 cut(s) 757
BstSCI CCNGG 2 cut(s) 321, 527
BstSFI CTRYAG 2 cut(s) 24, 407
BstUI CGCG 3 cut(s) 309, 482, 871
BstV1I GCAGC 3 cut(s) 162, 351, 479
BstV2I GAAGAC 1 cut(s) 1043
BstX2I RGATCY 4 cut(s) 484, 496, 1029, 1099
BstYI RGATCY 4 cut(s) 484, 496, 1029, 1099
BstZ17I GTATAC 1 cut(s) 747
Bsu15I ATCGAT 1 cut(s) 135
BsuI GTATCC 1 cut(s) 297
BsuRI GGCC 1 cut(s) 653
BsuTUI ATCGAT 1 cut(s) 135
BtgI CCRYGG 2 cut(s) 480, 781
BtrI CACGTC 1 cut(s) 386
BtsCI GGATG 5 cut(s) 298, 355, 733, 777, 1165
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 3 cut(s) 312, 463, 683
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 4 cut(s) 180, 305, 362, 1093
CaiI CAGNNNCTG 1 cut(s) 153
CfoI GCGC 2 cut(s) 309, 685
Cfr42I CCGCGG 1 cut(s) 483
ClaI ATCGAT 1 cut(s) 135
CseI GACGC 2 cut(s) 886, 1048
DdeI CTNAG 6 cut(s) 126, 146, 327, 438, 857, 1134
EaeI YGGCCR 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 781
Eco57I CTGAAG 1 cut(s) 906
EcoRII CCWGG 2 cut(s) 321, 527
EcoT14I CCWWGG 1 cut(s) 781
ErhI CCWWGG 1 cut(s) 781
FaqI GGGAC 1 cut(s) 77
FbaI TGATCA 1 cut(s) 558
FblI GTMKAC 2 cut(s) 746, 825
Fnu4HI GCNGC 6 cut(s) 37, 176, 310, 365, 468, 480
FokI GGATG 5 cut(s) 305, 362, 740, 764, 1172
Fsp4HI GCNGC 6 cut(s) 37, 176, 310, 365, 468, 480
FspBI CTAG 4 cut(s) 105, 183, 500, 750
GlaI GCGC 2 cut(s) 308, 684
GluI GCNGC 6 cut(s) 37, 176, 310, 365, 468, 480
HaeIII GGCC 1 cut(s) 653
HapII CCGG 2 cut(s) 290, 800
HgaI GACGC 2 cut(s) 886, 1048
HhaI GCGC 2 cut(s) 309, 685
Hin1I GRCGYC 1 cut(s) 1040
Hin6I GCGC 2 cut(s) 307, 683
HinP1I GCGC 2 cut(s) 307, 683
HinfI GANTC 6 cut(s) 20, 245, 524, 661, 786, 1113
HpaII CCGG 2 cut(s) 290, 800
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 3 cut(s) 435, 747, 826
Hpy188I TCNGA 9 cut(s) 47, 163, 383, 415, 666, 742, 886, 902, 924
Hpy188III TCNNGA 8 cut(s) 290, 536, 600, 800, 992, 1033, 1103, 1126
Hpy8I GTNNAC 3 cut(s) 435, 747, 826
Hpy99I CGWCG 1 cut(s) 387
HpyAV CCTTC 4 cut(s) 68, 845, 944, 998
HpyCH4III ACNGT 4 cut(s) 411, 428, 703, 830
HpyCH4IV ACGT 3 cut(s) 355, 385, 555
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 323, 476
HpyF3I CTNAG 6 cut(s) 126, 146, 327, 438, 857, 1134
HpySE526I ACGT 3 cut(s) 355, 385, 555
Hsp92I GRCGYC 1 cut(s) 1040
HspAI GCGC 2 cut(s) 307, 683
Kpn2I TCCGGA 2 cut(s) 289, 799
Ksp22I TGATCA 1 cut(s) 558
KspI CCGCGG 1 cut(s) 483
Lsp1109I GCAGC 3 cut(s) 162, 351, 479
LweI GCATC 2 cut(s) 127, 321
MaeI CTAG 4 cut(s) 105, 183, 500, 750
MaeII ACGT 3 cut(s) 355, 385, 555
MaeIII GTNAC 6 cut(s) 149, 386, 398, 568, 630, 863
MboII GAAGA 3 cut(s) 142, 937, 1048
MfeI CAATTG 1 cut(s) 912
MflI RGATCY 4 cut(s) 484, 496, 1029, 1099
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 8 cut(s) 58, 84, 169, 258, 275, 341, 816, 912
MluNI TGGCCA 1 cut(s) 653
MlyI GAGTC 1 cut(s) 795
MmeI TCCRAC 3 cut(s) 70, 393, 406
Mox20I TGGCCA 1 cut(s) 653
MroI TCCGGA 2 cut(s) 289, 799
MroXI GAANNNNTTC 1 cut(s) 987
MscI TGGCCA 1 cut(s) 653
MseI TTAA 2 cut(s) 168, 584
MslI CAYNNNNRTG 3 cut(s) 218, 519, 1001
Msp20I TGGCCA 1 cut(s) 653
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 2 cut(s) 290, 800
MspR9I CCNGG 2 cut(s) 323, 529
MunI CAATTG 1 cut(s) 912
Mva1269I GAATGC 2 cut(s) 367, 909
MvaI CCWGG 2 cut(s) 323, 529
MvnI CGCG 3 cut(s) 309, 482, 871
MwoI GCNNNNNNNGC 3 cut(s) 309, 323, 476
NcoI CCATGG 1 cut(s) 781
NmuCI GTSAC 2 cut(s) 386, 630
NspI RCATGY 2 cut(s) 53, 307
PaeI GCATGC 1 cut(s) 307
PaqCI CACCTGC 1 cut(s) 433
PciI ACATGT 1 cut(s) 49
PctI GAATGC 2 cut(s) 367, 909
PdmI GAANNNNTTC 1 cut(s) 987
PfeI GAWTC 5 cut(s) 20, 245, 524, 661, 1113
PkrI GCNGC 6 cut(s) 38, 177, 311, 366, 469, 481
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
Ppu21I YACGTR 1 cut(s) 556
PscI ACATGT 1 cut(s) 49
PshAI GACNNNNGTC 1 cut(s) 757
PsiI TTATAA 1 cut(s) 642
Psp6I CCWGG 2 cut(s) 321, 527
PspGI CCWGG 2 cut(s) 321, 527
PstNI CAGNNNCTG 1 cut(s) 153
PsuI RGATCY 4 cut(s) 484, 496, 1029, 1099
RseI CAYNNNNRTG 3 cut(s) 218, 519, 1001
SacII CCGCGG 1 cut(s) 483
SaqAI TTAA 2 cut(s) 168, 584
SatI GCNGC 6 cut(s) 37, 176, 310, 365, 468, 480
SchI GAGTC 1 cut(s) 795
ScrFI CCNGG 2 cut(s) 323, 529
SfaNI GCATC 2 cut(s) 127, 321
SfcI CTRYAG 2 cut(s) 24, 407
Sfr303I CCGCGG 1 cut(s) 483
SgrBI CCGCGG 1 cut(s) 483
SmiMI CAYNNNNRTG 3 cut(s) 218, 519, 1001
SmlI CTYRAG 2 cut(s) 1073, 1124
SmoI CTYRAG 2 cut(s) 1073, 1124
SphI GCATGC 1 cut(s) 307
Sse9I AATT 8 cut(s) 58, 84, 169, 258, 275, 341, 816, 912
SsiI CCGC 6 cut(s) 37, 206, 309, 480, 482, 506
SspMI CTAG 4 cut(s) 105, 183, 500, 750
StyD4I CCNGG 2 cut(s) 321, 527
StyI CCWWGG 1 cut(s) 781
TaaI ACNGT 4 cut(s) 411, 428, 703, 830
TaiI ACGT 3 cut(s) 358, 388, 558
TaqI TCGA 3 cut(s) 135, 599, 974
TasI AATT 8 cut(s) 58, 84, 169, 258, 275, 341, 816, 912
TauI GCSGC 3 cut(s) 39, 312, 482
TfiI GAWTC 5 cut(s) 20, 245, 524, 661, 1113
Tru1I TTAA 2 cut(s) 168, 584
Tru9I TTAA 2 cut(s) 168, 584
TscAI CASTG 3 cut(s) 319, 463, 683
TseFI GTSAC 2 cut(s) 386, 630
TseI GCWGC 3 cut(s) 175, 364, 467
Tsp45I GTSAC 2 cut(s) 386, 630
TspDTI ATGAA 6 cut(s) 180, 208, 366, 599, 611, 766
TspGWI ACGGA 1 cut(s) 740
TspRI CASTG 3 cut(s) 319, 463, 683
XapI RAATTY 3 cut(s) 84, 258, 275
XceI RCATGY 2 cut(s) 53, 307
XmiI GTMKAC 2 cut(s) 746, 825
XmnI GAANNNNTTC 1 cut(s) 987
XspI CTAG 4 cut(s) 105, 183, 500, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.