Rw4G019310

mitochondrial fission

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009680
Physical Location & Seq
Forward (+)
42582547 .. 42583785
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019310.1

Sequence Viewer

Length: 912 bp
ATGGGGAGAAAGAGAAAAAACAAATCATCAAGAGGTTCCAAAGAAAGAAAGACCAGATCAGAAGTGGGAGTAGCAAACCAAGATGCTGTAGTTCCTCGCCGCAAGATCCGACATGTCATCAATTCTTGCAAGGAGGAAGGGATTGAAATTCCAGTCCCAACCAGCGCTAGAGACTTGCCAGAGATTGCTAAGAAGATCGATGCCAAGCTCAGTTACTGCCTCTCGGAACTTAATTTGCTGCCTGCTAGTATTTCATCGGTTGATGAGGCGGTTGCCACTTTCATGCAACTGGTGGCATTGTCCAAAGAATCTCTCATAAAAATTCTTGTGAGAGGAGAATTTGATGAGTATCCTGATGACAAGAGCATGCACGGCACTGCAAACCTGGCTGAGATGCTCAATCAGTTCAAGGATGAACGTGGCTGGCTGCATTCCAGCTTTTTCCGACGTGACCTCAAAAGTAACTTCCTACAGTCGGAGATCAACTACTGTATGTTTACTCAGCAGGTGGGAAAAGCCAGTGGTCTTGCAGAGAAACTCGCCGCGGATCTTACAGCAGATCTAGTTGCGGTGGAACATCACCATGATTCCTGGTTTCTGGATATGTTCTGGTATTACGTGATCATGCAAGTAACTTTCCTTGATGATCCGGAAAAGCCCTCTACAATTATTGTAGACGGTATTGGGGAGATTGTGGTTGAAGGTCTTAGGAGTTACTCGCGTGATCTACTATCTGAAGCCTTTAGCTCGAGTGTTGCAAACCTTCTGGACACATACATGGATTTGGAGATGTTAGATATGCAGATCTTGAAGAGGCCACATTTTGCTCATGTATTTGAGGATATGAGAATCAATATGCTCAAGATAGCTAAGGAGATTTGTGAGGGGATCATGGATGCTTATGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Dynamin_N

Protein Analysis

303

Amino Acids

34.48

Weight (kDa)

5.4

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_M PF01031 58 - 165 2e-08 Dynamin central region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 496
Acc36I ACCTGC 1 cut(s) 496
AccI GTMKAC 1 cut(s) 675
AccII CGCG 2 cut(s) 545, 721
AccIII TCCGGA 1 cut(s) 649
AciI CCGC 5 cut(s) 100, 269, 543, 545, 569
AclWI GGATC 4 cut(s) 100, 555, 641, 896
AcsI RAATTY 3 cut(s) 147, 321, 338
AcuI CTGAAG 1 cut(s) 756
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 475
AflIII ACRYGT 1 cut(s) 112
AgsI TTSAA 4 cut(s) 146, 409, 701, 811
AjiI CACGTC 1 cut(s) 449
AjnI CCWGG 2 cut(s) 384, 590
AluBI AGCT 4 cut(s) 208, 438, 747, 869
AluI AGCT 4 cut(s) 208, 438, 747, 869
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 4 cut(s) 100, 555, 641, 896
AlwNI CAGNNNCTG 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 748
Aor13HI TCCGGA 1 cut(s) 649
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 1 cut(s) 815
ApeKI GCWGC 2 cut(s) 238, 427
ApoI RAATTY 3 cut(s) 147, 321, 338
AspLEI GCGC 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 572
AvaI CYCGRG 1 cut(s) 748
BbvI GCAGC 2 cut(s) 225, 414
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 2 cut(s) 386, 592
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 1 cut(s) 621
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 3 cut(s) 168, 246, 563
BfmI CTRYAG 2 cut(s) 87, 470
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 496
BfuI GTATCC 1 cut(s) 360
BglII AGATCT 2 cut(s) 559, 804
BisI GCNGC 4 cut(s) 100, 239, 428, 543
BlsI GCNGC 4 cut(s) 101, 240, 429, 544
Bme1390I CCNGG 2 cut(s) 386, 592
BmeT110I CYCGRG 1 cut(s) 748
BmgBI CACGTC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 2 cut(s) 386, 592
BmsI GCATC 4 cut(s) 73, 190, 384, 886
Bpu10I CCTNAGC 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 845
Bsa29I ATCGAT 1 cut(s) 198
BsaAI YACGTR 1 cut(s) 619
BsaBI GATNNNNATC 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 543
BsaWI WCCGGW 1 cut(s) 649
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse1I ACTGG 3 cut(s) 152, 294, 519
Bse8I GATNNNNATC 1 cut(s) 348
BseAI TCCGGA 1 cut(s) 649
BseBI CCWGG 2 cut(s) 386, 592
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 543
BseGI GGATG 2 cut(s) 418, 901
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMII CTCAG 3 cut(s) 223, 381, 515
BseNI ACTGG 3 cut(s) 152, 294, 519
BseRI GAGGAG 1 cut(s) 348
BseXI GCAGC 2 cut(s) 225, 414
Bsh1236I CGCG 2 cut(s) 545, 721
BshFI GGCC 1 cut(s) 817
BshVI ATCGAT 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 748
BsiSI CCGG 1 cut(s) 650
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 1 cut(s) 817
BsoBI CYCGRG 1 cut(s) 748
Bsp13I TCCGGA 1 cut(s) 649
BspACI CCGC 5 cut(s) 100, 269, 543, 545, 569
BspANI GGCC 1 cut(s) 817
BspCNI CTCAG 3 cut(s) 222, 382, 514
BspDI ATCGAT 1 cut(s) 198
BspEI TCCGGA 1 cut(s) 649
BspFNI CGCG 2 cut(s) 545, 721
BspLI GGNNCC 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 496
BspPI GGATC 4 cut(s) 100, 555, 641, 896
BsrI ACTGG 3 cut(s) 152, 294, 519
BssECI CCNNGG 1 cut(s) 543
Bst2UI CCWGG 2 cut(s) 386, 592
Bst4CI ACNGT 3 cut(s) 474, 491, 680
Bst6I CTCTTC 1 cut(s) 806
BstBAI YACGTR 1 cut(s) 619
BstC8I GCNNGC 3 cut(s) 243, 368, 425
BstDEI CTNAG 6 cut(s) 189, 209, 390, 501, 707, 870
BstDSI CCRYGG 1 cut(s) 543
BstF5I GGATG 2 cut(s) 418, 901
BstFNI CGCG 2 cut(s) 545, 721
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstMAI GTCTC 1 cut(s) 165
BstMWI GCNNNNNNNGC 2 cut(s) 372, 386
BstNI CCWGG 2 cut(s) 386, 592
BstNSI RCATGY 2 cut(s) 116, 370
BstSCI CCNGG 2 cut(s) 384, 590
BstSFI CTRYAG 2 cut(s) 87, 470
BstUI CGCG 2 cut(s) 545, 721
BstV1I GCAGC 2 cut(s) 225, 414
BstX2I RGATCY 4 cut(s) 105, 547, 559, 804
BstYI RGATCY 4 cut(s) 105, 547, 559, 804
Bsu15I ATCGAT 1 cut(s) 198
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 1 cut(s) 817
BsuTUI ATCGAT 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 543
BtrI CACGTC 1 cut(s) 449
BtsCI GGATG 2 cut(s) 418, 901
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 375, 526
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 3 cut(s) 243, 368, 425
CaiI CAGNNNCTG 1 cut(s) 216
CfoI GCGC 1 cut(s) 167
Cfr42I CCGCGG 1 cut(s) 546
ClaI ATCGAT 1 cut(s) 198
CviAII CATG 8 cut(s) 113, 283, 367, 584, 625, 778, 830, 892
DdeI CTNAG 6 cut(s) 189, 209, 390, 501, 707, 870
Eam1104I CTCTTC 1 cut(s) 806
EarI CTCTTC 1 cut(s) 806
Eco47III AGCGCT 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 756
Eco88I CYCGRG 1 cut(s) 748
EcoRII CCWGG 2 cut(s) 384, 590
FaeI CATG 8 cut(s) 116, 286, 370, 587, 628, 781, 833, 895
FaqI GGGAC 1 cut(s) 140
FatI CATG 8 cut(s) 112, 282, 366, 583, 624, 777, 829, 891
FbaI TGATCA 1 cut(s) 621
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 4 cut(s) 100, 239, 428, 543
FokI GGATG 2 cut(s) 425, 908
Fsp4HI GCNGC 4 cut(s) 100, 239, 428, 543
FspBI CTAG 3 cut(s) 168, 246, 563
GlaI GCGC 1 cut(s) 166
GluI GCNGC 4 cut(s) 100, 239, 428, 543
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 1 cut(s) 817
HapII CCGG 1 cut(s) 650
HhaI GCGC 1 cut(s) 167
Hin1II CATG 8 cut(s) 116, 286, 370, 587, 628, 781, 833, 895
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HinfI GANTC 3 cut(s) 308, 587, 849
HpaII CCGG 1 cut(s) 650
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 2 cut(s) 498, 676
Hpy188I TCNGA 6 cut(s) 61, 110, 226, 446, 478, 736
Hpy188III TCNNGA 7 cut(s) 30, 353, 599, 650, 767, 808, 862
Hpy8I GTNNAC 2 cut(s) 498, 676
Hpy99I CGWCG 1 cut(s) 450
HpyAV CCTTC 3 cut(s) 131, 695, 773
HpyCH4III ACNGT 3 cut(s) 474, 491, 680
HpyCH4IV ACGT 3 cut(s) 418, 448, 618
HpyCH4V TGCA 9 cut(s) 129, 286, 370, 380, 430, 530, 628, 758, 802
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 386
HpyF3I CTNAG 6 cut(s) 189, 209, 390, 501, 707, 870
HpySE526I ACGT 3 cut(s) 418, 448, 618
Hsp92II CATG 8 cut(s) 116, 286, 370, 587, 628, 781, 833, 895
HspAI GCGC 1 cut(s) 165
Kpn2I TCCGGA 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 621
KspI CCGCGG 1 cut(s) 546
Lsp1109I GCAGC 2 cut(s) 225, 414
LweI GCATC 4 cut(s) 73, 190, 384, 886
MaeI CTAG 3 cut(s) 168, 246, 563
MaeII ACGT 3 cut(s) 418, 448, 618
MaeIII GTNAC 5 cut(s) 212, 449, 461, 631, 713
MboII GAAGA 2 cut(s) 205, 823
MflI RGATCY 4 cut(s) 105, 547, 559, 804
MluCI AATT 6 cut(s) 121, 147, 232, 321, 338, 666
MmeI TCCRAC 3 cut(s) 133, 456, 469
MroI TCCGGA 1 cut(s) 649
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 3 cut(s) 281, 582, 776
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 1 cut(s) 650
MspR9I CCNGG 2 cut(s) 386, 592
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 2 cut(s) 386, 592
MvnI CGCG 2 cut(s) 545, 721
MwoI GCNNNNNNNGC 2 cut(s) 372, 386
NlaIII CATG 8 cut(s) 116, 286, 370, 587, 628, 781, 833, 895
NlaIV GGNNCC 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 449
NspI RCATGY 2 cut(s) 116, 370
PaeI GCATGC 1 cut(s) 370
PaeR7I CTCGAG 1 cut(s) 748
PaqCI CACCTGC 1 cut(s) 496
PciI ACATGT 1 cut(s) 112
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 3 cut(s) 308, 587, 849
PkrI GCNGC 4 cut(s) 101, 240, 429, 544
Ppu21I YACGTR 1 cut(s) 619
PscI ACATGT 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 384, 590
PspGI CCWGG 2 cut(s) 384, 590
PspN4I GGNNCC 1 cut(s) 37
PspXI VCTCGAGB 1 cut(s) 748
PstNI CAGNNNCTG 1 cut(s) 216
PsuI RGATCY 4 cut(s) 105, 547, 559, 804
RseI CAYNNNNRTG 3 cut(s) 281, 582, 776
SacII CCGCGG 1 cut(s) 546
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 4 cut(s) 100, 239, 428, 543
ScrFI CCNGG 2 cut(s) 386, 592
SfaNI GCATC 4 cut(s) 73, 190, 384, 886
SfcI CTRYAG 2 cut(s) 87, 470
Sfr274I CTCGAG 1 cut(s) 748
Sfr303I CCGCGG 1 cut(s) 546
SgrBI CCGCGG 1 cut(s) 546
SlaI CTCGAG 1 cut(s) 748
SmiMI CAYNNNNRTG 3 cut(s) 281, 582, 776
SmlI CTYRAG 2 cut(s) 748, 860
SmoI CTYRAG 2 cut(s) 748, 860
SphI GCATGC 1 cut(s) 370
Sse9I AATT 6 cut(s) 121, 147, 232, 321, 338, 666
SsiI CCGC 5 cut(s) 100, 269, 543, 545, 569
SspMI CTAG 3 cut(s) 168, 246, 563
StyD4I CCNGG 2 cut(s) 384, 590
TaaI ACNGT 3 cut(s) 474, 491, 680
TaiI ACGT 3 cut(s) 421, 451, 621
TaqI TCGA 2 cut(s) 198, 749
TasI AATT 6 cut(s) 121, 147, 232, 321, 338, 666
TauI GCSGC 2 cut(s) 102, 545
TfiI GAWTC 3 cut(s) 308, 587, 849
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 2 cut(s) 382, 526
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 2 cut(s) 238, 427
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 3 cut(s) 243, 271, 429
TspRI CASTG 2 cut(s) 382, 526
XapI RAATTY 3 cut(s) 147, 321, 338
XceI RCATGY 2 cut(s) 116, 370
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 748
XmiI GTMKAC 1 cut(s) 675
XspI CTAG 3 cut(s) 168, 246, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.