Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0002332

Physical Location & Seq

Genomic Coordinates Reverse (-)

25137803 .. 25142322

Gene Structure 4520 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000008067

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3117 bp

ATGGCGATACTCAAAACTTTTTTCCTTTCCACTCTTCTCCTCGCCTCAATCTCCTTCTCTCTTGCTTATCATCATGAGGATCCTTCCCAGCAGTTGGAGCAATGCCAGCGGCGATGCCAGGAGGAGATGCCGCGTGGTCTTCAACAGCAACAGTGCCAGCAAGAATGCAGAGAGCGGAAGCAGCAGCAGGGAGGCCGCGGTGGCGGTGGCAGAACCCCATTCTCTCGTGATCCTGAGCAGAAGTTTGAGCAGTGCGTGGAGAGTTGCCAGTCGAGGCAACACGGCCAGCAACAGCAGCAGCAATGCCGCCAACAATGCGGTCAACAAATGAGCCAGCACGAGCGCCAACAAATGTGCCAGCAGCGCTGTCAAAGGCAGCAACGTGGGGATTGGCAACAGCAGCAACACTGCCAAAGAAGGTGCGAGAGCCAATTTGAACAGCAACTGGAAGAACAAGACATTAACACATCAAGAGAAACGGAACAGCAATTTGAGGAGTGTCAGCAGAGCTGTCAATCCAGACAACACGGTCGCCAACAACAGCAACAATGTAGTCAGCAATGCGCTCAACAAACTTGCCAGCAGCATTGTCAGAGGCAACAAAGGGGGGATTGGCGCCAACAGCAACAGTGCCAACAGAGGTGTGAGAGGCAATTTGAACAGCGAGGAGACGAACTAGACATTAACACATCAAGAGATCCCGAACAAAAACTTGAGCAATGCCAACAGGGTTGCCAATTGAGGCAACAAGGCCAACAGCAGCAGCAGCAGTGCCGTCAACAATGCGCACAGCAATTGAGCCAGCGAGAGCGTCAACAAATCTGCCAAAGACGTTGTCAGATGCAAGACAGTGGTCAGCAACAGTGCCAAAGAAGGTGCATGCAGCAGCTCGAGCAAGGAGAACTAGACATTAACACATCAAGAGATCCCGAACAAAAACTTGAGCAATGCCAACAGAGTTGCCAATCGAGGCAACAAGGCCAACAACAGCAGCAGCAGTGCCGTCAACAATGCGCACAGCAATTGAGCCAGCAAGAGCGTCAACAAATCTGCCAAAGACGTTGTCAGATGCAAGACAGTGGTCAACAACAGTGCCAAAGAAGGTGCATGCAGCAGCTCGAGCAAGGAGAACTAGACATTAACACATCAAAGGATCCTGAACAAAAACTTGAGCAATGCCAACAGAGTTGCCAATCGAGGCAACAAGGCCAACAACAGCAGCAACAATGCCGTCAACAATGCGCACAGCAATTGAGCCAACGAGAGCGTCAACAAATCTGCCAAAGACGTTGTCAGATGCAAGACAGTGGTCAGCAACAGTGCCAAAGAAGGTGCATGCAGCAGCTCGAGCAAGGAGAACTAGACATTAACACATCAAGAGATCCCGAACAAAAAATTGAGCAATGCCAACAGAGTTGCCAATCGCGGCAACAAGGCCAACAACAGCAGCAGCAGTGCCGTCAACAATGCGCAAAGCAATTGAGCCAGCAAGAGCGTCAACAAACCTGCCAAAGACGTTGTCAGATGCAAGACAGTGGTCAGCAACAGTGCCAAAGAAGGTGCATGCAGCAGCTCGAGCAAGGAGAACAAGACATTAACACATCAAGAGATCCTGAACAAAAACTTGAGCAATGTCACCAGGGTTGCCAATCAAGGGAACAAGGCCAACAAGAGCAGCAGCAGCAGTGCCGTCAGCAATGTGCACAGCAATTGAGCCAGCGAGAGCGTCAACAAATCTGCGAGCAACGGTGCCAGAGGCAACAACGTGGGGATCAACAACAGCAACAACAGTGCCAAAGAAGGTGCCAGCAACAACTTGAGCAGGGTCAACAGGGTCAGGGCCAGAACTACCGACGAGAAAGGGAGGACCAGCAAAGTCGGGGTCAGAGCCAAATGAATAACCCTTACTACTTTCCCTCCGACATGTTTCAGCAAAAATTCAGGTCTCAAGAAGGTGGGATGTATGTCCTTGAGAGGTTCACCAAGAATGAAAATAAGCTTCTTCGCGGCATTAGGAATTACCGGTTGGCCATCATCGAGGCCAAGCCCAACACTTTCGTCCTCCCACACCATTGTGATGCTGAATCTATTTTCGTTGTTATCAATGGACGTTGCGCCTGTACAATGTTGATGCAAGACAACAAAGAGTCGTTCAACATGGAAATGGGTGATGTCCTCAGAGTTCCTGCAGGAGCTACCGTTTACCTTGTTAACAACAACAATGATCAGACTCTAAGAATTGTAAAGCTCAAGCAGCCAGTCAACACTCCTGGACGATTTGAGGAATTCTTCCCTGCAAGTTCTCAAGACCTAGACTCATACTTCAACGTTTTCAGCGATGACATTCTTGAATCAGCCTTCAATACCCCAAGTGATCAGCTACAGAGGGTGTTTGGACAACAACAGCAGAAGAGGCAAGGCATGGTGATGAGGGCATCACCACAACAACTCCAGGCATTGAGTCAGCACGCTTCTTCACCCAGGAGCAAAGGACGTCAGTCTGGCAAGGGTCCATTCAATCTTCGAAACCAAAAGCCCATCCAGGCCAACGAGTTCGGCAAATTATTCGAAGCTCGCCCTGAGAAGTTCGATCAGCTCCAGGACATGGATGTCTCAGTCACTTGCGTTGAAGTCAACAATGAGGCTATGATGCTGCCGCACTACAATTCTAAAGCAATATTCGTGGTGCTGGTTGTGGAAGGAAGCGGACGGGTCGAGATGGCATGCCCACACATGGCAAGCCAGAGCCAAATGAGTGAGGAAGAAGTAGAACAACAAGGAGAACAGGTGAAGGTCACAGCTGATGTATCAGAGGATGATGTTTTCGTTGTGCCAGCAGGTCATCCCATTGCCATAGTTGCCCAAAACCAACAATTGAAAATGTTGGGATTTGGAATCAATGCCCAGAACAACAAGAGGAACTTCATTGCAGGGAGAGAGGCTAACCCGATTAGGGAGATGGAGAGAGAGGCTAAGCAACTGACCTTCGGGCAAGAGATGGAGCAGATCATCAACAGGCAGAGGGAATCCTATTTCGCCCCGACAGGGCAGAGCCAGCAGCAGAGAGGAGGCAGGGACGAGCCCTTATCTTCAATTTTGGCATTTGCAGGCTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009615 GO:0009620 GO:0009719 GO:0009725 GO:0009735 GO:0010033 GO:0030312 GO:0031410 GO:0031982 GO:0033095 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0097708 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_1 Vicilin_N

Protein Analysis

1039

Amino Acids

121.96

Weight (kDa)

7.83

Isoelectric Point (pI)

79.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_1	PF00190	668 - 752	3.8e-07	Cupin
Cupin_1	PF00190	839 - 978	1.2e-21	Cupin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002332)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22640
fragaria_vesca	FvH4_2g23190 FvH4_4g15931 FvH4_4g15931
malus_domestica	MD03G1261200.v1.1 MD11G1140200.v1.1 MD11G1281600.v1.1 MD13G1196800.v1.1 MD16G1197100.v1.1 MD16G1197200.v1.1
prunus_persica	Prupe.1G017700_v2.0.a1 Prupe.1G018000_v2.0.a1 Prupe.8G237500_v2.0.a1
pyrus_communis	pycom09g17330 pycom11g24970 pycom13g16920 pycom13g16930 pycom16g16590 pycom16g16620
rosa_chinensis	RchiOBHm_Chr4g0415511 RchiOBHm_Chr6g0290441
rosa_laevigata	RLG00000008067 RLG00000012215
rosa_multiflora	Rmu_sc0002890.1_g000006 Rmu_sc0007265.1_g000001 Rmu_sc0007625.1_g000002 Rmu_sc0020894.1_g000005 Rmu_sc0020894.1_g000006
rosa_roxburghii	Rroxscaffold_5G00359290 Rroxscaffold_7G00177060
rosa_rugosa	Rorug04G0134600 Rorug06G0212700
rosa_samantha	Rh4AG195500 Rh4BG194300 Rh4CG206400 Rh4DG193300 Rh6AG322900 Rh6BG330300 Rh6CG336400 Rh6DG323300
rosa_wichuraiana	Rw4G016650 Rw6G027990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	528, 2609
AatII	GACGTC	1 cut(s)	2497
Acc16I	TGCGCA	4 cut(s)	787, 1015, 1243, 1471
Acc36I	ACCTGC	2 cut(s)	1514, 2828
AccB1I	GGYRCC	3 cut(s)	615, 1749, 1803
AccB7I	CCANNNNNTGG	2 cut(s)	94, 2605
AccBSI	CCGCTC	1 cut(s)	175
AccII	CGCG	4 cut(s)	133, 198, 1426, 2007
AclI	AACGTT	1 cut(s)	2328
AcoI	YGGCCR	2 cut(s)	283, 2028
AcsI	RAATTY	2 cut(s)	1937, 2285
AcyI	GRCGYC	2 cut(s)	616, 2494
AfaI	GTAC	1 cut(s)	2122
AfeI	AGCGCT	1 cut(s)	365
AfiI	CCNNNNNNNGG	6 cut(s)	94, 1654, 2605, 2734, 2953, 3045
AflIII	ACRYGT	1 cut(s)	1923
AgeI	ACCGGT	1 cut(s)	2022
AjnI	CCWGG	7 cut(s)	117, 1638, 2269, 2451, 2480, 2541, 2598
AjuI	GAANNNNNNNTTGG	2 cut(s)	2009, 2041
AleI	CACNNNNGTG	1 cut(s)	2073
Alw21I	GWGCWC	1 cut(s)	1705
Alw26I	GTCTC	3 cut(s)	663, 1950, 2617
Alw44I	GTGCAC	1 cut(s)	1701
AlwNI	CAGNNNCTG	1 cut(s)	445
Ama87I	CYCGRG	4 cut(s)	890, 1118, 1346, 1574
Aor51HI	AGCGCT	1 cut(s)	365
ApaLI	GTGCAC	1 cut(s)	1701
ApoI	RAATTY	2 cut(s)	1937, 2285
AsiGI	ACCGGT	1 cut(s)	2022
AspLEI	GCGC	9 cut(s)	345, 366, 566, 618, 788, 1016, 1244, 1472, 2117
AspS9I	GGNCC	3 cut(s)	1840, 1867, 2510
AsuHPI	GGTGA	7 cut(s)	1628, 1972, 2180, 2430, 2437, 2469, 2800
AsuII	TTCGAA	2 cut(s)	2524, 2568
AvaI	CYCGRG	4 cut(s)	890, 1118, 1346, 1574
AvaII	GGWCC	2 cut(s)	1867, 2510
BaeGI	GKGCMC	1 cut(s)	1705
BalI	TGGCCA	1 cut(s)	2030
BamHI	GGATCC	2 cut(s)	79, 1153
BanI	GGYRCC	3 cut(s)	615, 1749, 1803
BanII	GRGCYC	1 cut(s)	3084
BauI	CACGAG	2 cut(s)	225, 338
BbsI	GAAGAC	1 cut(s)	131
Bbv12I	GWGCWC	1 cut(s)	1705
BccI	CCATC	5 cut(s)	2039, 2546, 2713, 2953, 2992
BceAI	ACGGC	6 cut(s)	298, 759, 987, 1215, 1443, 1674
BcgI	CGANNNNNNTGC	2 cut(s)	2548, 2582
BciT130I	CCWGG	7 cut(s)	119, 1640, 2271, 2453, 2482, 2543, 2600
BclI	TGATCA	2 cut(s)	2224, 2374
BcoDI	GTCTC	3 cut(s)	663, 1950, 2617
BfaI	CTAG	5 cut(s)	677, 905, 1133, 1361, 2312
BfmI	CTRYAG	2 cut(s)	2187, 2381
BfoI	RGCGCY	3 cut(s)	346, 367, 619
BfuAI	ACCTGC	2 cut(s)	1514, 2828
BglI	GCCNNNNNGGC	1 cut(s)	201
BlpI	GCTNAGC	1 cut(s)	2973
Bme1390I	CCNGG	7 cut(s)	119, 1640, 2271, 2453, 2482, 2543, 2600
Bme18I	GGWCC	2 cut(s)	1867, 2510
BmeT110I	CYCGRG	4 cut(s)	890, 1118, 1346, 1574
BmgT120I	GGNCC	3 cut(s)	1840, 1867, 2510
BmiI	GGNNCC	6 cut(s)	81, 617, 1155, 1751, 1805, 2511
BmrFI	CCNGG	7 cut(s)	119, 1640, 2271, 2453, 2482, 2543, 2600
BoxI	GACNNNNGTC	5 cut(s)	852, 1080, 1308, 1536, 2497
BpiI	GAAGAC	1 cut(s)	131
BpmI	CTGGAG	2 cut(s)	2435, 2582
Bpu10I	CCTNAGC	1 cut(s)	234
Bpu1102I	GCTNAGC	1 cut(s)	2973
Bpu14I	TTCGAA	2 cut(s)	2524, 2568
BpuEI	CTTGAG	9 cut(s)	734, 962, 1190, 1646, 1838, 1932, 1991, 2234, 2289
BsaHI	GRCGYC	2 cut(s)	616, 2494
BsaI	GGTCTC	1 cut(s)	1950
BsaJI	CCNNGG	3 cut(s)	196, 1639, 2480
BsaWI	WCCGGW	1 cut(s)	2022
BsaXI	ACNNNNNCTCC	8 cut(s)	1901, 1931, 2184, 2214, 2433, 2463, 2772, 2802
Bsc4I	CCNNNNNNNGG	6 cut(s)	94, 1654, 2605, 2734, 2953, 3045
Bse118I	RCCGGY	1 cut(s)	2022
Bse1I	ACTGG	3 cut(s)	268, 450, 2258
BseBI	CCWGG	7 cut(s)	119, 1640, 2271, 2453, 2482, 2543, 2600
BseDI	CCNNGG	3 cut(s)	196, 1639, 2480
BseGI	GGATG	5 cut(s)	1965, 2538, 2614, 2821, 2842
BseLI	CCNNNNNNNGG	6 cut(s)	94, 1654, 2605, 2734, 2953, 3045
BseMII	CTCAG	4 cut(s)	225, 2191, 2571, 2628
BseNI	ACTGG	3 cut(s)	268, 450, 2258
BseRI	GAGGAG	5 cut(s)	29, 137, 509, 681, 3081
BseSI	GKGCMC	1 cut(s)	1705
BseYI	CCCAGC	1 cut(s)	87
Bsh1236I	CGCG	4 cut(s)	133, 198, 1426, 2007
Bsh1285I	CGRYCG	1 cut(s)	532
BshNI	GGYRCC	3 cut(s)	615, 1749, 1803
BshTI	ACCGGT	1 cut(s)	2022
BsiEI	CGRYCG	1 cut(s)	532
BsiHKAI	GWGCWC	1 cut(s)	1705
BsiHKCI	CYCGRG	4 cut(s)	890, 1118, 1346, 1574
BsiSI	CCGG	1 cut(s)	2023
BslFI	GGGAC	1 cut(s)	3089
BslI	CCNNNNNNNGG	6 cut(s)	94, 1654, 2605, 2734, 2953, 3045
BsmAI	GTCTC	3 cut(s)	663, 1950, 2617
BsmBI	CGTCTC	1 cut(s)	663
BsmFI	GGGAC	1 cut(s)	3089
BsmI	GAATGC	1 cut(s)	170
Bso31I	GGTCTC	1 cut(s)	1950
BsoBI	CYCGRG	4 cut(s)	890, 1118, 1346, 1574
Bsp119I	TTCGAA	2 cut(s)	2524, 2568
Bsp1286I	GDGCHC	2 cut(s)	1705, 3084
Bsp1407I	TGTACA	1 cut(s)	2120
Bsp1720I	GCTNAGC	1 cut(s)	2973
BspCNI	CTCAG	4 cut(s)	226, 2190, 2572, 2627
BspFNI	CGCG	4 cut(s)	133, 198, 1426, 2007
BspHI	TCATGA	1 cut(s)	73
BspLI	GGNNCC	6 cut(s)	81, 617, 1155, 1751, 1805, 2511
BspMAI	CTGCAG	1 cut(s)	2191
BspMI	ACCTGC	2 cut(s)	1514, 2828
BspT104I	TTCGAA	2 cut(s)	2524, 2568
BspT107I	GGYRCC	3 cut(s)	615, 1749, 1803
BspTNI	GGTCTC	1 cut(s)	1950
BsrBI	CCGCTC	1 cut(s)	175
BsrFI	RCCGGY	1 cut(s)	2022
BsrGI	TGTACA	1 cut(s)	2120
BsrI	ACTGG	3 cut(s)	268, 450, 2258
BssAI	RCCGGY	1 cut(s)	2022
BssECI	CCNNGG	3 cut(s)	196, 1639, 2480
BssNI	GRCGYC	2 cut(s)	616, 2494
BssSI	CACGAG	2 cut(s)	225, 338
Bst2BI	CACGAG	2 cut(s)	225, 338
Bst2UI	CCWGG	7 cut(s)	119, 1640, 2271, 2453, 2482, 2543, 2600
Bst6I	CTCTTC	2 cut(s)	39, 2405
BstACI	GRCGYC	2 cut(s)	616, 2494
BstAUI	TGTACA	1 cut(s)	2120
BstBI	TTCGAA	2 cut(s)	2524, 2568
BstDEI	CTNAG	6 cut(s)	234, 2177, 2234, 2580, 2614, 2973
BstDSI	CCRYGG	1 cut(s)	196
BstF5I	GGATG	5 cut(s)	1965, 2538, 2614, 2821, 2842
BstFNI	CGCG	4 cut(s)	133, 198, 1426, 2007
BstH2I	RGCGCY	3 cut(s)	346, 367, 619
BstHHI	GCGC	9 cut(s)	345, 366, 566, 618, 788, 1016, 1244, 1472, 2117
BstMAI	GTCTC	3 cut(s)	663, 1950, 2617
BstMCI	CGRYCG	1 cut(s)	532
BstNI	CCWGG	7 cut(s)	119, 1640, 2271, 2453, 2482, 2543, 2600
BstNSI	RCATGY	6 cut(s)	883, 1111, 1339, 1567, 1927, 2727
BstPAI	GACNNNNGTC	5 cut(s)	852, 1080, 1308, 1536, 2497
BstSCI	CCNGG	7 cut(s)	117, 1638, 2269, 2451, 2480, 2541, 2598
BstSFI	CTRYAG	2 cut(s)	2187, 2381
BstSLI	GKGCMC	1 cut(s)	1705
BstUI	CGCG	4 cut(s)	133, 198, 1426, 2007
BstV2I	GAAGAC	1 cut(s)	131
BstX2I	RGATCY	6 cut(s)	79, 697, 925, 1153, 1381, 1609
BstYI	RGATCY	6 cut(s)	79, 697, 925, 1153, 1381, 1609
BtgI	CCRYGG	1 cut(s)	196
BtgZI	GCGATG	2 cut(s)	127, 2352
BtsCI	GGATG	5 cut(s)	1965, 2538, 2614, 2821, 2842
BtsI	GCAGTG	6 cut(s)	257, 406, 776, 1004, 1460, 1691
BveI	ACCTGC	2 cut(s)	1514, 2828
CaiI	CAGNNNCTG	1 cut(s)	445
CciI	TCATGA	1 cut(s)	73
CfoI	GCGC	9 cut(s)	345, 366, 566, 618, 788, 1016, 1244, 1472, 2117
Cfr10I	RCCGGY	1 cut(s)	2022
Cfr13I	GGNCC	3 cut(s)	1840, 1867, 2510
Cfr42I	CCGCGG	1 cut(s)	199
CseI	GACGC	5 cut(s)	800, 1028, 1256, 1484, 1715
Csp6I	GTAC	1 cut(s)	2121
CspAI	ACCGGT	1 cut(s)	2022
CspCI	CAANNNNNGTGG	2 cut(s)	2664, 2699
CviQI	GTAC	1 cut(s)	2121
DdeI	CTNAG	6 cut(s)	234, 2177, 2234, 2580, 2614, 2973
DinI	GGCGCC	1 cut(s)	617
DrdI	GACNNNNNNGTC	2 cut(s)	528, 2609
DseDI	GACNNNNNNGTC	2 cut(s)	528, 2609
EaeI	YGGCCR	2 cut(s)	283, 2028
Eam1104I	CTCTTC	2 cut(s)	39, 2405
EarI	CTCTTC	2 cut(s)	39, 2405
Eco24I	GRGCYC	1 cut(s)	3084
Eco31I	GGTCTC	1 cut(s)	1950
Eco47I	GGWCC	2 cut(s)	1867, 2510
Eco47III	AGCGCT	1 cut(s)	365
Eco88I	CYCGRG	4 cut(s)	890, 1118, 1346, 1574
EcoRI	GAATTC	1 cut(s)	2285
EcoRII	CCWGG	7 cut(s)	117, 1638, 2269, 2451, 2480, 2541, 2598
EcoT38I	GRGCYC	1 cut(s)	3084
EgeI	GGCGCC	1 cut(s)	617
EheI	GGCGCC	1 cut(s)	617
Esp3I	CGTCTC	1 cut(s)	663
FalI	AAGNNNNNCTT	2 cut(s)	2906, 2938
FaqI	GGGAC	1 cut(s)	3089
FbaI	TGATCA	2 cut(s)	2224, 2374
FokI	GGATG	5 cut(s)	1972, 2525, 2621, 2828, 2829
FriOI	GRGCYC	1 cut(s)	3084
FspAI	RTGCGCAY	3 cut(s)	787, 1015, 1243
FspBI	CTAG	5 cut(s)	677, 905, 1133, 1361, 2312
FspI	TGCGCA	4 cut(s)	787, 1015, 1243, 1471
GlaI	GCGC	9 cut(s)	344, 365, 565, 617, 787, 1015, 1243, 1471, 2116
GsaI	CCCAGC	1 cut(s)	91
GsuI	CTGGAG	2 cut(s)	2435, 2582
HaeII	RGCGCY	3 cut(s)	346, 367, 619
HapII	CCGG	1 cut(s)	2023
HgaI	GACGC	5 cut(s)	800, 1028, 1256, 1484, 1715
HhaI	GCGC	9 cut(s)	345, 366, 566, 618, 788, 1016, 1244, 1472, 2117
Hin1I	GRCGYC	2 cut(s)	616, 2494
Hin6I	GCGC	9 cut(s)	343, 364, 564, 616, 786, 1014, 1242, 1470, 2115
HinP1I	GCGC	9 cut(s)	343, 364, 564, 616, 786, 1014, 1242, 1470, 2115
HindIII	AAGCTT	1 cut(s)	1997
HinfI	GANTC	8 cut(s)	2084, 2147, 2230, 2315, 2351, 2461, 2895, 3026
HpaI	GTTAAC	1 cut(s)	2212
HpaII	CCGG	1 cut(s)	2023
HphI	GGTGA	7 cut(s)	1628, 1972, 2180, 2430, 2437, 2469, 2800
Hpy99I	CGWCG	1 cut(s)	1857
HpyCH4IV	ACGT	9 cut(s)	382, 832, 1060, 1288, 1516, 1765, 2110, 2328, 2494
HpyF3I	CTNAG	6 cut(s)	234, 2177, 2234, 2580, 2614, 2973
HpySE526I	ACGT	9 cut(s)	382, 832, 1060, 1288, 1516, 1765, 2110, 2328, 2494
Hsp92I	GRCGYC	2 cut(s)	616, 2494
HspAI	GCGC	9 cut(s)	343, 364, 564, 616, 786, 1014, 1242, 1470, 2115
KasI	GGCGCC	1 cut(s)	615
Ksp22I	TGATCA	2 cut(s)	2224, 2374
KspAI	GTTAAC	1 cut(s)	2212
KspI	CCGCGG	1 cut(s)	199
LmnI	GCTCC	5 cut(s)	97, 2192, 2484, 2601, 3001
MaeI	CTAG	5 cut(s)	677, 905, 1133, 1361, 2312
MaeII	ACGT	9 cut(s)	382, 832, 1060, 1288, 1516, 1765, 2110, 2328, 2494
MaeIII	GTNAC	3 cut(s)	1634, 2617, 2794
MbiI	CCGCTC	1 cut(s)	175
MfeI	CAATTG	7 cut(s)	737, 794, 1022, 1250, 1478, 1709, 2873
MflI	RGATCY	6 cut(s)	79, 697, 925, 1153, 1381, 1609
MhlI	GDGCHC	2 cut(s)	1705, 3084
MlsI	TGGCCA	1 cut(s)	2030
MluNI	TGGCCA	1 cut(s)	2030
Mly113I	GGCGCC	1 cut(s)	616
MlyI	GAGTC	4 cut(s)	2156, 2224, 2309, 2470
MmeI	TCCRAC	2 cut(s)	75, 1944
Mox20I	TGGCCA	1 cut(s)	2030
MscI	TGGCCA	1 cut(s)	2030
MseI	TTAA	8 cut(s)	462, 684, 912, 1140, 1368, 1596, 2211, 3115
MslI	CAYNNNNRTG	4 cut(s)	2073, 2076, 2162, 2426
Msp20I	TGGCCA	1 cut(s)	2030
MspA1I	CMGCKG	3 cut(s)	109, 198, 2801
MspI	CCGG	1 cut(s)	2023
MspR9I	CCNGG	7 cut(s)	119, 1640, 2271, 2453, 2482, 2543, 2600
MunI	CAATTG	7 cut(s)	737, 794, 1022, 1250, 1478, 1709, 2873
Mva1269I	GAATGC	1 cut(s)	170
MvaI	CCWGG	7 cut(s)	119, 1640, 2271, 2453, 2482, 2543, 2600
MvnI	CGCG	4 cut(s)	133, 198, 1426, 2007
NarI	GGCGCC	1 cut(s)	616
NlaIV	GGNNCC	6 cut(s)	81, 617, 1155, 1751, 1805, 2511
NmuCI	GTSAC	3 cut(s)	1634, 2617, 2794
NsbI	TGCGCA	4 cut(s)	787, 1015, 1243, 1471
NspI	RCATGY	6 cut(s)	883, 1111, 1339, 1567, 1927, 2727
NspV	TTCGAA	2 cut(s)	2524, 2568
OliI	CACNNNNGTG	1 cut(s)	2073
PaeI	GCATGC	5 cut(s)	883, 1111, 1339, 1567, 2727
PaeR7I	CTCGAG	4 cut(s)	890, 1118, 1346, 1574
PagI	TCATGA	1 cut(s)	73
PciI	ACATGT	1 cut(s)	1923
PcsI	WCGNNNNNNNCGW	1 cut(s)	2334
PctI	GAATGC	1 cut(s)	170
PfeI	GAWTC	4 cut(s)	2084, 2351, 2895, 3026
PflFI	GACNNNGTC	4 cut(s)	834, 1062, 1290, 1518
PflMI	CCANNNNNTGG	2 cut(s)	94, 2605
PfoI	TCCNGGA	2 cut(s)	2269, 2598
PinAI	ACCGGT	1 cut(s)	2022
PleI	GAGTC	4 cut(s)	2155, 2224, 2309, 2469
PluTI	GGCGCC	1 cut(s)	619
PpsI	GAGTC	4 cut(s)	2155, 2224, 2309, 2469
PscI	ACATGT	1 cut(s)	1923
PshAI	GACNNNNGTC	5 cut(s)	852, 1080, 1308, 1536, 2497
Psp1406I	AACGTT	1 cut(s)	2328
Psp6I	CCWGG	7 cut(s)	117, 1638, 2269, 2451, 2480, 2541, 2598
PspFI	CCCAGC	1 cut(s)	87
PspGI	CCWGG	7 cut(s)	117, 1638, 2269, 2451, 2480, 2541, 2598
PspN4I	GGNNCC	6 cut(s)	81, 617, 1155, 1751, 1805, 2511
PspPI	GGNCC	3 cut(s)	1840, 1867, 2510
PspXI	VCTCGAGB	4 cut(s)	890, 1118, 1346, 1574
PstI	CTGCAG	1 cut(s)	2191
PstNI	CAGNNNCTG	1 cut(s)	445
PsuI	RGATCY	6 cut(s)	79, 697, 925, 1153, 1381, 1609
PsyI	GACNNNGTC	4 cut(s)	834, 1062, 1290, 1518
PvuII	CAGCTG	1 cut(s)	2801
RsaI	GTAC	1 cut(s)	2122
RsaNI	GTAC	1 cut(s)	2121
RseI	CAYNNNNRTG	4 cut(s)	2073, 2076, 2162, 2426
SacII	CCGCGG	1 cut(s)	199
SaqAI	TTAA	8 cut(s)	462, 684, 912, 1140, 1368, 1596, 2211, 3115
Sau96I	GGNCC	3 cut(s)	1840, 1867, 2510
SbfI	CCTGCAGG	1 cut(s)	2191
SchI	GAGTC	4 cut(s)	2156, 2224, 2309, 2470
ScrFI	CCNGG	7 cut(s)	119, 1640, 2271, 2453, 2482, 2543, 2600
SdaI	CCTGCAGG	1 cut(s)	2191
SduI	GDGCHC	2 cut(s)	1705, 3084
SfcI	CTRYAG	2 cut(s)	2187, 2381
SfoI	GGCGCC	1 cut(s)	617
Sfr274I	CTCGAG	4 cut(s)	890, 1118, 1346, 1574
Sfr303I	CCGCGG	1 cut(s)	199
SfuI	TTCGAA	2 cut(s)	2524, 2568
SgrBI	CCGCGG	1 cut(s)	199
SinI	GGWCC	2 cut(s)	1867, 2510
SlaI	CTCGAG	4 cut(s)	890, 1118, 1346, 1574
SmiMI	CAYNNNNRTG	4 cut(s)	2073, 2076, 2162, 2426
SphI	GCATGC	5 cut(s)	883, 1111, 1339, 1567, 2727
Sse8387I	CCTGCAGG	1 cut(s)	2191
SspDI	GGCGCC	1 cut(s)	615
SspI	AATATT	1 cut(s)	2679
SspMI	CTAG	5 cut(s)	677, 905, 1133, 1361, 2312
StyD4I	CCNGG	7 cut(s)	117, 1638, 2269, 2451, 2480, 2541, 2598
TaiI	ACGT	9 cut(s)	385, 835, 1063, 1291, 1519, 1768, 2113, 2331, 2497
TatI	WGTACW	1 cut(s)	2120
TauI	GCSGC	7 cut(s)	112, 133, 198, 309, 1429, 2010, 2659
TfiI	GAWTC	4 cut(s)	2084, 2351, 2895, 3026
Tru1I	TTAA	8 cut(s)	462, 684, 912, 1140, 1368, 1596, 2211, 3115
Tru9I	TTAA	8 cut(s)	462, 684, 912, 1140, 1368, 1596, 2211, 3115
TseFI	GTSAC	3 cut(s)	1634, 2617, 2794
Tsp45I	GTSAC	3 cut(s)	1634, 2617, 2794
TspDTI	ATGAA	3 cut(s)	1910, 2004, 2914
TspGWI	ACGGA	1 cut(s)	494
Tth111I	GACNNNGTC	4 cut(s)	834, 1062, 1290, 1518
Van91I	CCANNNNNTGG	2 cut(s)	94, 2605
VneI	GTGCAC	1 cut(s)	1701
VpaK11BI	GGWCC	2 cut(s)	1867, 2510
XapI	RAATTY	2 cut(s)	1937, 2285
XceI	RCATGY	6 cut(s)	883, 1111, 1339, 1567, 1927, 2727
XhoI	CTCGAG	4 cut(s)	890, 1118, 1346, 1574
XspI	CTAG	5 cut(s)	677, 905, 1133, 1361, 2312
ZraI	GACGTC	1 cut(s)	2495

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.