Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0002332

Physical Location & Seq

Genomic Coordinates Forward (+)

16305117 .. 16307495

Gene Structure 2379 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000012215

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGAGTTGAAAAGTAAGCTTTGTTTGGTTGTCTTGCTCTTCTCTGTTTTGCTTGCTGTGAGTGCTAGTCAGGACTATATGGAGAGAGACCCTGAGCTGAAGCAGTGCAAGCACCAGTGCAAACACCAGAGAGGATATGATGAGCAAGAGAAGCAACTCTGCGAGCAGCGATGCGATGACTACATCAAGCAGAAGAGGCAGGAAGAGAAGCAGCGAAGGAGAGAAGGAGAAGGAGGTACTAGTTTTTATAGATATGACGAGGATCAGTATCAGGGTCAGGGCCAACAACAACAGGACCAGAATCCCTACTTGTTTGAAGATCAGGATTTCGAGACCCAAATTGAAACAGATCAAGGCCGAGTTCAGATTCTTCAGAAGTTCACTGACAAGTCTGATCTGCTGCGCGGCATTGAGAATTTCCGGATTGGTTCTCTTGTCACCAAGCCTCACTCTTTTGTTGCTCCACACCATCATGATGCTGACTGTGTTCTCTTCGTCTTCCAGGGTCGACCAACCATTACTATGGTGAGGGGGGAGAAGAGAGAGAGCCATAACCTTGAACGTGGAGACATACTCAGGGTTCCAGCTGGTACACCTGTTTATATTGTCAATAGAGACGAGAATGAGAAGCTGTTTGTTGTGAAACTCCTCCACCCAGTCTCTCTTCCTGATCAGTTTGAGACATTTTATGAGGGAGGTGGTGAAAACCCGGAATCATTTTTCAAAGCTTTCAGTCCGGAGGTACTTCAAGCGGCTTTCAAGACTGAAAGAGATGAGCTGGAGAGGCTATTTGGGCAGCAGAGGCAAGGCAGCATCATGAAGGCCTCCAGAGAGCAAATTGAGAAGATGAGCCACAGTACTCGTGGCCGTGAAGGATTTTGGCCTTTCCATGGTGATGAACCATCTTCAACATCATCATCGAAATCAGGCCCATTCAATCTCTTCAAAAAGCGGCCTTCTCAGTCAAACCGACACGGACGTCTCTTTGAAGCCGATTCCAATGACTACAAACAGCTCGAGGAGCTCGACCTCCAAATCTCTTTTGCTAACATTACTCAAGGGTCCATGACTGCTCCATCCTACAACTCCAAAGCCACCAAGATTGCCTTTGTTTTGGATGGTGAGGGCTTCTTCGAGATGGCCTGCCCCCACCTTGCTTCCCAAGGAAGCTCCCAAGGAGGTTCCCAACACCAACAGCAACGTCAGCGGGGGAGCAGCAGCCCAAGCTACCAGAACGTAAGAGGGGACTTGAGACGCGGTACCGTTTTTATTGTGCTGGCTGGCCATCCGGTAACTGTCATTGCCTCGAGCAACAATAACCTACAAGTCATCTGCTTTGAGGTGAATGCGAAAGACAATGTGAGGTTTCCCCTGGCTGGAAAGAAGAATGTGGTGAGTCTGTTTGAGAGGGAGGCAAAGGAGTTGGCATTCGGTGTTCCGGTGAGAGAGGTGGACCGCATCTTTAACAAGCAAGATGGCGAGTTTTTTCTGGAGGGACCGAACAATCAGCAGCAGGGTCGTATGTGGTCAGTGGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0016020 GO:0019863 GO:0019865 GO:0044877

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_1

Protein Analysis

513

Amino Acids

58.59

Weight (kDa)

6.21

Isoelectric Point (pI)

51.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_1	PF00190	145 - 257	8.8e-10	Cupin
Cupin_2	PF07883	149 - 208	8.9e-06	Cupin domain
Cupin_1	PF00190	312 - 480	5.3e-24	Cupin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002332)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22640
fragaria_vesca	FvH4_2g23190 FvH4_4g15931 FvH4_4g15931
malus_domestica	MD03G1261200.v1.1 MD11G1140200.v1.1 MD11G1281600.v1.1 MD13G1196800.v1.1 MD16G1197100.v1.1 MD16G1197200.v1.1
prunus_persica	Prupe.1G017700_v2.0.a1 Prupe.1G018000_v2.0.a1 Prupe.8G237500_v2.0.a1
pyrus_communis	pycom09g17330 pycom11g24970 pycom13g16920 pycom13g16930 pycom16g16590 pycom16g16620
rosa_chinensis	RchiOBHm_Chr4g0415511 RchiOBHm_Chr6g0290441
rosa_laevigata	RLG00000008067 RLG00000012215
rosa_multiflora	Rmu_sc0002890.1_g000006 Rmu_sc0007265.1_g000001 Rmu_sc0007625.1_g000002 Rmu_sc0020894.1_g000005 Rmu_sc0020894.1_g000006
rosa_roxburghii	Rroxscaffold_5G00359290 Rroxscaffold_7G00177060
rosa_rugosa	Rorug04G0134600 Rorug06G0212700
rosa_samantha	Rh4AG195500 Rh4BG194300 Rh4CG206400 Rh4DG193300 Rh6AG322900 Rh6BG330300 Rh6CG336400 Rh6DG323300
rosa_wichuraiana	Rw4G016650 Rw6G027990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	978
AatII	GACGTC	1 cut(s)	982
Acc65I	GGTACC	1 cut(s)	1259
AccB1I	GGYRCC	1 cut(s)	1259
AccI	GTMKAC	1 cut(s)	508
AccII	CGCG	2 cut(s)	405, 1257
AccIII	TCCGGA	2 cut(s)	420, 736
AciI	CCGC	6 cut(s)	405, 752, 952, 1207, 1257, 1456
AclWI	GGATC	1 cut(s)	270
AcoI	YGGCCR	2 cut(s)	865, 1282
AcsI	RAATTY	1 cut(s)	415
AcuI	CTGAAG	2 cut(s)	119, 356
AcyI	GRCGYC	1 cut(s)	979
AfaI	GTAC	5 cut(s)	238, 592, 744, 859, 1261
AfiI	CCNNNNNNNGG	2 cut(s)	890, 1376
AhlI	ACTAGT	1 cut(s)	239
AjnI	CCWGG	2 cut(s)	501, 1371
Alw21I	GWGCWC	1 cut(s)	1026
Alw26I	GTCTC	8 cut(s)	81, 326, 561, 609, 664, 674, 986, 1246
AlwI	GGATC	1 cut(s)	270
Ama87I	CYCGRG	2 cut(s)	1016, 1306
Aor13HI	TCCGGA	2 cut(s)	420, 736
AoxI	GGCC	9 cut(s)	280, 355, 822, 865, 881, 928, 953, 1140, 1282
ApeKI	GCWGC	8 cut(s)	166, 211, 400, 796, 810, 1215, 1218, 1510
ApoI	RAATTY	1 cut(s)	415
Asp718I	GGTACC	1 cut(s)	1259
AspLEI	GCGC	1 cut(s)	405
AspS9I	GGNCC	6 cut(s)	280, 295, 929, 1062, 1453, 1496
AsuC2I	CCSGG	1 cut(s)	710
AsuHPI	GGTGA	8 cut(s)	430, 538, 713, 905, 1133, 1354, 1405, 1453
AvaI	CYCGRG	2 cut(s)	1016, 1306
AvaII	GGWCC	4 cut(s)	295, 1062, 1453, 1496
BaeI	ACNNNNGTAYC	2 cut(s)	582, 615
BalI	TGGCCA	1 cut(s)	1284
BanI	GGYRCC	1 cut(s)	1259
BanII	GRGCYC	1 cut(s)	1026
BauI	CACGAG	1 cut(s)	861
BbsI	GAAGAC	1 cut(s)	490
Bbv12I	GWGCWC	1 cut(s)	1026
BbvI	GCAGC	8 cut(s)	178, 223, 387, 808, 822, 1227, 1230, 1522
BccI	CCATC	7 cut(s)	477, 910, 1084, 1112, 1132, 1293, 1469
BceAI	ACGGC	1 cut(s)	852
BcgI	CGANNNNNNTGC	2 cut(s)	1499, 1533
BciT130I	CCWGG	2 cut(s)	503, 1373
BclI	TGATCA	1 cut(s)	670
BcnI	CCSGG	1 cut(s)	710
BcoDI	GTCTC	8 cut(s)	81, 326, 561, 609, 664, 674, 986, 1246
BcuI	ACTAGT	1 cut(s)	239
BfaI	CTAG	2 cut(s)	66, 240
BmcAI	AGTACT	1 cut(s)	859
Bme1390I	CCNGG	3 cut(s)	503, 710, 1373
Bme18I	GGWCC	4 cut(s)	295, 1062, 1453, 1496
BmeT110I	CYCGRG	2 cut(s)	1016, 1306
BmgT120I	GGNCC	6 cut(s)	280, 295, 929, 1062, 1453, 1496
BmiI	GGNNCC	5 cut(s)	582, 1063, 1183, 1261, 1497
BmrFI	CCNGG	3 cut(s)	503, 710, 1373
BmrI	ACTGGG	1 cut(s)	650
BmsI	GCATC	4 cut(s)	161, 466, 822, 1467
BmuI	ACTGGG	1 cut(s)	650
BpiI	GAAGAC	1 cut(s)	490
BplI	GAGNNNNNCTC	2 cut(s)	558, 590
BpmI	CTGGAG	3 cut(s)	800, 811, 1511
Bpu10I	CCTNAGC	1 cut(s)	93
BpuEI	CTTGAG	2 cut(s)	1041, 1270
BpuMI	CCSGG	1 cut(s)	710
BsaBI	GATNNNNATC	1 cut(s)	267
BsaHI	GRCGYC	1 cut(s)	979
BsaI	GGTCTC	2 cut(s)	81, 326
BsaJI	CCNNGG	5 cut(s)	502, 889, 1162, 1174, 1371
BsaWI	WCCGGW	4 cut(s)	420, 736, 1288, 1438
Bsc4I	CCNNNNNNNGG	2 cut(s)	890, 1376
Bse1I	ACTGG	2 cut(s)	115, 656
Bse3DI	GCAATG	1 cut(s)	1299
Bse8I	GATNNNNATC	1 cut(s)	267
BseAI	TCCGGA	2 cut(s)	420, 736
BseBI	CCWGG	2 cut(s)	503, 1373
BseDI	CCNNGG	5 cut(s)	502, 889, 1162, 1174, 1371
BseGI	GGATG	3 cut(s)	1076, 1123, 1285
BseJI	GATNNNNATC	1 cut(s)	267
BseLI	CCNNNNNNNGG	2 cut(s)	890, 1376
BseMI	GCAATG	1 cut(s)	1299
BseMII	CTCAG	3 cut(s)	84, 589, 974
BseNI	ACTGG	2 cut(s)	115, 656
BseRI	GAGGAG	2 cut(s)	638, 1034
BseXI	GCAGC	8 cut(s)	178, 223, 387, 808, 822, 1227, 1230, 1522
Bsh1236I	CGCG	2 cut(s)	405, 1257
BshFI	GGCC	9 cut(s)	282, 357, 824, 867, 883, 930, 955, 1142, 1284
BshNI	GGYRCC	1 cut(s)	1259
BsiHKAI	GWGCWC	1 cut(s)	1026
BsiHKCI	CYCGRG	2 cut(s)	1016, 1306
BsiSI	CCGG	5 cut(s)	421, 710, 737, 1289, 1439
BslFI	GGGAC	2 cut(s)	1259, 1509
BslI	CCNNNNNNNGG	2 cut(s)	890, 1376
BsmAI	GTCTC	8 cut(s)	81, 326, 561, 609, 664, 674, 986, 1246
BsmBI	CGTCTC	3 cut(s)	609, 986, 1246
BsmFI	GGGAC	2 cut(s)	1259, 1509
BsmI	GAATGC	2 cut(s)	1351, 1427
BsnI	GGCC	9 cut(s)	282, 357, 824, 867, 883, 930, 955, 1142, 1284
Bso31I	GGTCTC	2 cut(s)	81, 326
BsoBI	CYCGRG	2 cut(s)	1016, 1306
Bsp1286I	GDGCHC	1 cut(s)	1026
Bsp13I	TCCGGA	2 cut(s)	420, 736
Bsp143I	GATC	5 cut(s)	262, 319, 349, 394, 670
Bsp19I	CCATGG	1 cut(s)	889
BspACI	CCGC	6 cut(s)	405, 752, 952, 1207, 1257, 1456
BspANI	GGCC	9 cut(s)	282, 357, 824, 867, 883, 930, 955, 1142, 1284
BspCNI	CTCAG	3 cut(s)	85, 588, 973
BspEI	TCCGGA	2 cut(s)	420, 736
BspFNI	CGCG	2 cut(s)	405, 1257
BspHI	TCATGA	2 cut(s)	472, 816
BspLI	GGNNCC	5 cut(s)	582, 1063, 1183, 1261, 1497
BspPI	GGATC	1 cut(s)	270
BspQI	GCTCTTC	1 cut(s)	44
BspT107I	GGYRCC	1 cut(s)	1259
BspTNI	GGTCTC	2 cut(s)	81, 326
BsrDI	GCAATG	1 cut(s)	1299
BsrI	ACTGG	2 cut(s)	115, 656
BssECI	CCNNGG	5 cut(s)	502, 889, 1162, 1174, 1371
BssMI	GATC	5 cut(s)	262, 319, 349, 394, 670
BssNI	GRCGYC	1 cut(s)	979
BssSI	CACGAG	1 cut(s)	861
BssT1I	CCWWGG	3 cut(s)	889, 1162, 1174
Bst2BI	CACGAG	1 cut(s)	861
Bst2UI	CCWGG	2 cut(s)	503, 1373
Bst4CI	ACNGT	4 cut(s)	485, 857, 1264, 1297
Bst6I	CTCTTC	7 cut(s)	44, 188, 198, 497, 533, 669, 947
BstACI	GRCGYC	1 cut(s)	979
BstC8I	GCNNGC	6 cut(s)	54, 110, 164, 1144, 1278, 1282
BstDEI	CTNAG	3 cut(s)	93, 575, 960
BstDSI	CCRYGG	1 cut(s)	889
BstF5I	GGATG	3 cut(s)	1076, 1123, 1285
BstFNI	CGCG	2 cut(s)	405, 1257
BstHHI	GCGC	1 cut(s)	405
BstKTI	GATC	5 cut(s)	265, 322, 352, 397, 673
BstMAI	GTCTC	8 cut(s)	81, 326, 561, 609, 664, 674, 986, 1246
BstMBI	GATC	5 cut(s)	262, 319, 349, 394, 670
BstNI	CCWGG	2 cut(s)	503, 1373
BstSCI	CCNGG	3 cut(s)	501, 708, 1371
BstUI	CGCG	2 cut(s)	405, 1257
BstV1I	GCAGC	8 cut(s)	178, 223, 387, 808, 822, 1227, 1230, 1522
BstV2I	GAAGAC	1 cut(s)	490
BstXI	CCANNNNNNTGG	1 cut(s)	523
BsuRI	GGCC	9 cut(s)	282, 357, 824, 867, 883, 930, 955, 1142, 1284
BtgI	CCRYGG	1 cut(s)	889
BtgZI	GCGATG	2 cut(s)	184, 189
BtsCI	GGATG	3 cut(s)	1076, 1123, 1285
BtsI	GCAGTG	1 cut(s)	110
BtsIMutI	CAGTG	4 cut(s)	110, 122, 381, 1536
Cac8I	GCNNGC	6 cut(s)	54, 110, 164, 1144, 1278, 1282
CciI	TCATGA	2 cut(s)	472, 816
CfoI	GCGC	1 cut(s)	405
Cfr13I	GGNCC	6 cut(s)	280, 295, 929, 1062, 1453, 1496
CseI	GACGC	1 cut(s)	1263
Csp6I	GTAC	5 cut(s)	237, 591, 743, 858, 1260
CspCI	CAANNNNNGTGG	2 cut(s)	1084, 1119
CviAII	CATG	4 cut(s)	473, 817, 890, 1066
CviQI	GTAC	5 cut(s)	237, 591, 743, 858, 1260
DdeI	CTNAG	3 cut(s)	93, 575, 960
DpnI	GATC	5 cut(s)	264, 321, 351, 396, 672
DpnII	GATC	5 cut(s)	262, 319, 349, 394, 670
DrdI	GACNNNNNNGTC	1 cut(s)	978
DseDI	GACNNNNNNGTC	1 cut(s)	978
EaeI	YGGCCR	2 cut(s)	865, 1282
Eam1104I	CTCTTC	7 cut(s)	44, 188, 198, 497, 533, 669, 947
EarI	CTCTTC	7 cut(s)	44, 188, 198, 497, 533, 669, 947
Ecl136II	GAGCTC	1 cut(s)	1024
Eco130I	CCWWGG	3 cut(s)	889, 1162, 1174
Eco147I	AGGCCT	1 cut(s)	824
Eco24I	GRGCYC	1 cut(s)	1026
Eco31I	GGTCTC	2 cut(s)	81, 326
Eco47I	GGWCC	4 cut(s)	295, 1062, 1453, 1496
Eco53kI	GAGCTC	1 cut(s)	1024
Eco57I	CTGAAG	2 cut(s)	119, 356
Eco88I	CYCGRG	2 cut(s)	1016, 1306
EcoICRI	GAGCTC	1 cut(s)	1024
EcoRII	CCWGG	2 cut(s)	501, 1371
EcoT14I	CCWWGG	3 cut(s)	889, 1162, 1174
EcoT38I	GRGCYC	1 cut(s)	1026
ErhI	CCWWGG	3 cut(s)	889, 1162, 1174
Esp3I	CGTCTC	3 cut(s)	609, 986, 1246
FaeI	CATG	4 cut(s)	476, 820, 893, 1069
FalI	AAGNNNNNCTT	2 cut(s)	1091, 1123
FaqI	GGGAC	2 cut(s)	1259, 1509
FatI	CATG	4 cut(s)	472, 816, 889, 1065
FauI	CCCGC	1 cut(s)	1200
FbaI	TGATCA	1 cut(s)	670
FblI	GTMKAC	1 cut(s)	508
FokI	GGATG	3 cut(s)	1063, 1130, 1272
FriOI	GRGCYC	1 cut(s)	1026
FspBI	CTAG	2 cut(s)	66, 240
GlaI	GCGC	1 cut(s)	404
GsuI	CTGGAG	3 cut(s)	800, 811, 1511
HaeIII	GGCC	9 cut(s)	282, 357, 824, 867, 883, 930, 955, 1142, 1284
HapII	CCGG	5 cut(s)	421, 710, 737, 1289, 1439
HgaI	GACGC	1 cut(s)	1263
HhaI	GCGC	1 cut(s)	405
Hin1I	GRCGYC	1 cut(s)	979
Hin1II	CATG	4 cut(s)	476, 820, 893, 1069
Hin6I	GCGC	1 cut(s)	403
HinP1I	GCGC	1 cut(s)	403
HincII	GTYRAC	1 cut(s)	509
HindII	GTYRAC	1 cut(s)	509
HindIII	AAGCTT	2 cut(s)	17, 726
HinfI	GANTC	5 cut(s)	301, 367, 713, 995, 1396
HpaII	CCGG	5 cut(s)	421, 710, 737, 1289, 1439
HphI	GGTGA	8 cut(s)	430, 538, 713, 905, 1133, 1354, 1405, 1453
Hpy166II	GTNNAC	4 cut(s)	381, 509, 593, 1453
Hpy188I	TCNGA	3 cut(s)	366, 375, 394
Hpy8I	GTNNAC	4 cut(s)	381, 509, 593, 1453
HpyAV	CCTTC	6 cut(s)	210, 218, 224, 814, 866, 966
HpyCH4III	ACNGT	4 cut(s)	485, 857, 1264, 1297
HpyCH4IV	ACGT	4 cut(s)	562, 979, 1201, 1236
HpyCH4V	TGCA	2 cut(s)	108, 120
HpyF3I	CTNAG	3 cut(s)	93, 575, 960
HpySE526I	ACGT	4 cut(s)	562, 979, 1201, 1236
Hsp92I	GRCGYC	1 cut(s)	979
Hsp92II	CATG	4 cut(s)	476, 820, 893, 1069
HspAI	GCGC	1 cut(s)	403
Kpn2I	TCCGGA	2 cut(s)	420, 736
KpnI	GGTACC	1 cut(s)	1263
Ksp22I	TGATCA	1 cut(s)	670
Kzo9I	GATC	5 cut(s)	262, 319, 349, 394, 670
LguI	GCTCTTC	1 cut(s)	44
LmnI	GCTCC	5 cut(s)	466, 1021, 1078, 1175, 1212
Lsp1109I	GCAGC	8 cut(s)	178, 223, 387, 808, 822, 1227, 1230, 1522
LweI	GCATC	4 cut(s)	161, 466, 822, 1467
MaeI	CTAG	2 cut(s)	66, 240
MaeII	ACGT	4 cut(s)	562, 979, 1201, 1236
MaeIII	GTNAC	2 cut(s)	436, 1291
MalI	GATC	5 cut(s)	264, 321, 351, 396, 672
MboI	GATC	5 cut(s)	262, 319, 349, 394, 670
MhlI	GDGCHC	1 cut(s)	1026
MlsI	TGGCCA	1 cut(s)	1284
MluCI	AATT	3 cut(s)	339, 415, 837
MluNI	TGGCCA	1 cut(s)	1284
MlyI	GAGTC	1 cut(s)	1405
Mox20I	TGGCCA	1 cut(s)	1284
MroI	TCCGGA	2 cut(s)	420, 736
MscI	TGGCCA	1 cut(s)	1284
MseI	TTAA	1 cut(s)	1464
MslI	CAYNNNNRTG	4 cut(s)	471, 474, 521, 894
Msp20I	TGGCCA	1 cut(s)	1284
MspA1I	CMGCKG	2 cut(s)	587, 1207
MspI	CCGG	5 cut(s)	421, 710, 737, 1289, 1439
MspR9I	CCNGG	3 cut(s)	503, 710, 1373
Mva1269I	GAATGC	2 cut(s)	1351, 1427
MvaI	CCWGG	2 cut(s)	503, 1373
MvnI	CGCG	2 cut(s)	405, 1257
NciI	CCSGG	1 cut(s)	710
NcoI	CCATGG	1 cut(s)	889
NdeII	GATC	5 cut(s)	262, 319, 349, 394, 670
NlaIII	CATG	4 cut(s)	476, 820, 893, 1069
NlaIV	GGNNCC	5 cut(s)	582, 1063, 1183, 1261, 1497
NmeAIII	GCCGAG	1 cut(s)	383
NmuCI	GTSAC	1 cut(s)	436
PaeR7I	CTCGAG	2 cut(s)	1016, 1306
PagI	TCATGA	2 cut(s)	472, 816
PceI	AGGCCT	1 cut(s)	824
PciSI	GCTCTTC	1 cut(s)	44
PcsI	WCGNNNNNNNCGW	1 cut(s)	1023
PctI	GAATGC	2 cut(s)	1351, 1427
PfeI	GAWTC	4 cut(s)	301, 367, 713, 995
PleI	GAGTC	1 cut(s)	1404
PpsI	GAGTC	1 cut(s)	1404
Psp124BI	GAGCTC	1 cut(s)	1026
Psp6I	CCWGG	2 cut(s)	501, 1371
PspGI	CCWGG	2 cut(s)	501, 1371
PspN4I	GGNNCC	5 cut(s)	582, 1063, 1183, 1261, 1497
PspPI	GGNCC	6 cut(s)	280, 295, 929, 1062, 1453, 1496
PspXI	VCTCGAGB	2 cut(s)	1016, 1306
PvuII	CAGCTG	1 cut(s)	587
RsaI	GTAC	5 cut(s)	238, 592, 744, 859, 1261
RsaNI	GTAC	5 cut(s)	237, 591, 743, 858, 1260
RseI	CAYNNNNRTG	4 cut(s)	471, 474, 521, 894
SacI	GAGCTC	1 cut(s)	1026
SalI	GTCGAC	1 cut(s)	507
SapI	GCTCTTC	1 cut(s)	44
SaqAI	TTAA	1 cut(s)	1464
Sau3AI	GATC	5 cut(s)	262, 319, 349, 394, 670
Sau96I	GGNCC	6 cut(s)	280, 295, 929, 1062, 1453, 1496
ScaI	AGTACT	1 cut(s)	859
SchI	GAGTC	1 cut(s)	1405
ScrFI	CCNGG	3 cut(s)	503, 710, 1373
SduI	GDGCHC	1 cut(s)	1026
SfaNI	GCATC	4 cut(s)	161, 466, 822, 1467
Sfr274I	CTCGAG	2 cut(s)	1016, 1306
SinI	GGWCC	4 cut(s)	295, 1062, 1453, 1496
SlaI	CTCGAG	2 cut(s)	1016, 1306
SmiMI	CAYNNNNRTG	4 cut(s)	471, 474, 521, 894
SmlI	CTYRAG	4 cut(s)	1016, 1056, 1249, 1306
SmoI	CTYRAG	4 cut(s)	1016, 1056, 1249, 1306
SpeI	ACTAGT	1 cut(s)	239
Sse9I	AATT	3 cut(s)	339, 415, 837
SseBI	AGGCCT	1 cut(s)	824
SsiI	CCGC	6 cut(s)	405, 752, 952, 1207, 1257, 1456
SspMI	CTAG	2 cut(s)	66, 240
SstI	GAGCTC	1 cut(s)	1026
StuI	AGGCCT	1 cut(s)	824
StyD4I	CCNGG	3 cut(s)	501, 708, 1371
StyI	CCWWGG	3 cut(s)	889, 1162, 1174
TaaI	ACNGT	4 cut(s)	485, 857, 1264, 1297
TaiI	ACGT	4 cut(s)	565, 982, 1204, 1239
TaqI	TCGA	7 cut(s)	330, 508, 920, 1017, 1026, 1134, 1307
TaqII	GACCGA	1 cut(s)	1513
TasI	AATT	3 cut(s)	339, 415, 837
TatI	WGTACW	1 cut(s)	857
TauI	GCSGC	3 cut(s)	408, 755, 955
TfiI	GAWTC	4 cut(s)	301, 367, 713, 995
Tru1I	TTAA	1 cut(s)	1464
Tru9I	TTAA	1 cut(s)	1464
TscAI	CASTG	4 cut(s)	110, 122, 388, 1536
TseFI	GTSAC	1 cut(s)	436
TseI	GCWGC	8 cut(s)	166, 211, 400, 796, 810, 1215, 1218, 1510
Tsp45I	GTSAC	1 cut(s)	436
TspDTI	ATGAA	2 cut(s)	833, 912
TspGWI	ACGGA	1 cut(s)	990
TspRI	CASTG	4 cut(s)	110, 122, 388, 1536
VpaK11BI	GGWCC	4 cut(s)	295, 1062, 1453, 1496
XapI	RAATTY	1 cut(s)	415
XcmI	CCANNNNNNNNNTGG	1 cut(s)	860
XhoI	CTCGAG	2 cut(s)	1016, 1306
XmiI	GTMKAC	1 cut(s)	508
XspI	CTAG	2 cut(s)	66, 240
ZraI	GACGTC	1 cut(s)	980
ZrmI	AGTACT	1 cut(s)	859

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.