RLG00000014473

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000777
Physical Location & Seq
Reverse (-)
53592537 .. 53593945
1409 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014473

Sequence Viewer

Length: 1155 bp
ATGTCTTGGATGGCACCTACATGCCTTCGACATCCTTCACCGGTAGCTATCGCGTCTGCGCCTATCTCGACCCCCTCAGAACCGAACCACGCTTCTCTCAAGCGCTTCTTCTTGTCTCTACTCGCCAGTAGCCACCACAGGTTAATCCCACTCTTCCAAAACCACTTGTCCGACCTCTTTCTCAACCTGGAAACTGAAATTTCAAAAATTGGCAAAGCAGACGTCAACACCTCGAGCAACAACATGTCCTTTAATTTCATTTTCTCGCTGTTATGTGGATCAGGAAGTACTTCATTGGGTTCCAAAGGGCCGACCCTGGTGGCGGTATGGCTAGCGCCGCAACTTGCACCACAGATCACACTCGGGTTGCCCAAGTTGCTGAGCTTTTCTGAAGATTTCTTGCTGCACACGTTTTCCTACCCTGCATTTTTTGTCAAATCGACCTACAAGAAGCTCTACGATGCGTTTTACGAGTTGTCGGCTTCGGTTTTGGATGAGGCTGAGAATAGTTTTGGGATTCCGAGAGACGAAGCTTGCCACAACCTTTTGTTTTTGGCTTGCTTCAATGCTTTTGGTGGCATGAAGATTTTGTTCCCTGCTTTAATCAAGTGGGTTGGATCCACAGGAGAGGATTTGCACCACCAGCTCCGAAATGAAATCAGGGCCGTTGTTAAGGAAAATGATGGGAATGTTACTTTTTCGGCGTTGGATAAGATGACTTTGACCAAGTCTGTGGTTTATGAGGCTTTGAGGATTGAACCTCCGGTTCTGTTCCAATACGGGAAGGCCAAGGAGGATATGGTGGTCCATAGCCATGATGCAACATTTGAGATCAAGAAAGGGGAAATGATCTTCAGATATCAACCGTTCGCCATGAAGGATCCGAAGATTTTCGAGAATCCGAAGGAGTTTGTGGGGCATAGGTTTATAGGAGAAGGTGAGAAACTGTTGAAGTATATTTATTGGTCGAATGGTCGCGAGACTGATGATCCAACAGTGGAGAATAAGCAGTGTGCCGGAAAGGATTTGGTGTTGCTTGTGTCTAGGTTAATGTTGGTAGAGTTTTTCCTTCGGTATGACACTTGTACCATTGATGTGACTATAGTTTCATCGGGTACTTTAGTGACGTGCAGAACATTGACCAAAGCCTCTTGA

Protein Analysis

385

Amino Acids

43.0

Weight (kDa)

6.64

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 212 - 319 6.2e-16 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 13
AccII CGCG 2 cut(s) 53, 978
AciI CCGC 2 cut(s) 323, 338
AclWI GGATC 6 cut(s) 286, 612, 625, 875, 888, 983
AcsI RAATTY 1 cut(s) 198
AcuI CTGAAG 2 cut(s) 411, 838
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 3 cut(s) 289, 1087, 1117
AfeI AGCGCT 1 cut(s) 104
AfiI CCNNNNNNNGG 1 cut(s) 322
AflIII ACRYGT 2 cut(s) 243, 408
AgeI ACCGGT 1 cut(s) 40
AgsI TTSAA 4 cut(s) 204, 565, 758, 952
AjiI CACGTC 1 cut(s) 1128
AjnI CCWGG 2 cut(s) 186, 315
AluBI AGCT 5 cut(s) 47, 384, 454, 533, 646
AluI AGCT 5 cut(s) 47, 384, 454, 533, 646
Alw26I GTCTC 3 cut(s) 120, 519, 974
AlwI GGATC 6 cut(s) 286, 612, 625, 875, 888, 983
Ama87I CYCGRG 2 cut(s) 232, 362
Aor51HI AGCGCT 1 cut(s) 104
AoxI GGCC 3 cut(s) 308, 663, 786
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 1 cut(s) 198
ArsI GACNNNNNNTTYG 2 cut(s) 207, 239
AsiGI ACCGGT 1 cut(s) 40
Asp700I GAANNNNTTC 2 cut(s) 289, 890
AspLEI GCGC 3 cut(s) 61, 105, 337
AspS9I GGNCC 3 cut(s) 308, 663, 805
AsuHPI GGTGA 2 cut(s) 30, 950
AsuNHI GCTAGC 1 cut(s) 331
AvaI CYCGRG 2 cut(s) 232, 362
AvaII GGWCC 1 cut(s) 805
BaeI ACNNNNGTAYC 4 cut(s) 1069, 1102, 1107, 1140
BamHI GGATCC 2 cut(s) 617, 880
BanI GGYRCC 1 cut(s) 13
BbvI GCAGC 1 cut(s) 390
BccI CCATC 2 cut(s) 4, 677
BceAI ACGGC 1 cut(s) 650
BciT130I CCWGG 2 cut(s) 188, 317
BcoDI GTCTC 3 cut(s) 120, 519, 974
BfaI CTAG 2 cut(s) 332, 1044
BfmI CTRYAG 1 cut(s) 1101
BfoI RGCGCY 2 cut(s) 106, 338
BisI GCNGC 2 cut(s) 338, 404
BlpI GCTNAGC 1 cut(s) 380
BlsI GCNGC 2 cut(s) 339, 405
BmcAI AGTACT 1 cut(s) 289
Bme1390I CCNGG 2 cut(s) 188, 317
Bme18I GGWCC 1 cut(s) 805
BmeT110I CYCGRG 2 cut(s) 232, 362
BmgBI CACGTC 1 cut(s) 1128
BmgT120I GGNCC 3 cut(s) 308, 663, 805
BmiI GGNNCC 4 cut(s) 15, 301, 619, 882
BmrFI CCNGG 2 cut(s) 188, 317
BmsI GCATC 2 cut(s) 451, 808
BmtI GCTAGC 1 cut(s) 335
Bpu1102I GCTNAGC 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 83
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 315, 789
BsaWI WCCGGW 2 cut(s) 40, 763
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 126
BseBI CCWGG 2 cut(s) 188, 317
BseDI CCNNGG 2 cut(s) 315, 789
BseGI GGATG 3 cut(s) 15, 31, 499
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMII CTCAG 3 cut(s) 90, 371, 492
BseNI ACTGG 1 cut(s) 126
BseXI GCAGC 1 cut(s) 390
BsgI GTGCAG 2 cut(s) 389, 1150
Bsh1236I CGCG 2 cut(s) 53, 978
BshFI GGCC 3 cut(s) 310, 665, 788
BshNI GGYRCC 1 cut(s) 13
BshTI ACCGGT 1 cut(s) 40
BsiHKCI CYCGRG 2 cut(s) 232, 362
BsiSI CCGG 3 cut(s) 41, 764, 1017
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 3 cut(s) 120, 519, 974
BsmBI CGTCTC 1 cut(s) 519
BsnI GGCC 3 cut(s) 310, 665, 788
BsoBI CYCGRG 2 cut(s) 232, 362
Bsp143I GATC 7 cut(s) 278, 354, 617, 831, 849, 880, 988
Bsp1720I GCTNAGC 1 cut(s) 380
Bsp68I TCGCGA 1 cut(s) 978
BspACI CCGC 2 cut(s) 323, 338
BspANI GGCC 3 cut(s) 310, 665, 788
BspCNI CTCAG 3 cut(s) 89, 372, 493
BspFNI CGCG 2 cut(s) 53, 978
BspLI GGNNCC 4 cut(s) 15, 301, 619, 882
BspOI GCTAGC 1 cut(s) 335
BspPI GGATC 6 cut(s) 286, 612, 625, 875, 888, 983
BspT107I GGYRCC 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 1 cut(s) 126
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 315, 789
BssMI GATC 7 cut(s) 278, 354, 617, 831, 849, 880, 988
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 789
Bst2UI CCWGG 2 cut(s) 188, 317
Bst4CI ACNGT 3 cut(s) 867, 948, 997
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 222
BstC8I GCNNGC 3 cut(s) 333, 535, 559
BstDEI CTNAG 3 cut(s) 76, 380, 501
BstF5I GGATG 3 cut(s) 15, 31, 499
BstFNI CGCG 2 cut(s) 53, 978
BstH2I RGCGCY 2 cut(s) 106, 338
BstHHI GCGC 3 cut(s) 61, 105, 337
BstKTI GATC 7 cut(s) 281, 357, 620, 834, 852, 883, 991
BstMAI GTCTC 3 cut(s) 120, 519, 974
BstMBI GATC 7 cut(s) 278, 354, 617, 831, 849, 880, 988
BstMWI GCNNNNNNNGC 3 cut(s) 337, 376, 643
BstNI CCWGG 2 cut(s) 188, 317
BstNSI RCATGY 2 cut(s) 24, 247
BstSCI CCNGG 2 cut(s) 186, 315
BstSFI CTRYAG 1 cut(s) 1101
BstUI CGCG 2 cut(s) 53, 978
BstV1I GCAGC 1 cut(s) 390
BstX2I RGATCY 2 cut(s) 617, 880
BstXI CCANNNNNNTGG 1 cut(s) 733
BstYI RGATCY 2 cut(s) 617, 880
BsuRI GGCC 3 cut(s) 310, 665, 788
BtrI CACGTC 1 cut(s) 1128
BtsCI GGATG 3 cut(s) 15, 31, 499
BtsI GCAGTG 1 cut(s) 1016
BtsIMutI CAGTG 2 cut(s) 1002, 1016
BtuMI TCGCGA 1 cut(s) 978
Cac8I GCNNGC 3 cut(s) 333, 535, 559
CfoI GCGC 3 cut(s) 61, 105, 337
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 308, 663, 805
CseI GACGC 1 cut(s) 42
Csp6I GTAC 3 cut(s) 288, 1086, 1116
CspAI ACCGGT 1 cut(s) 40
CviAII CATG 5 cut(s) 21, 244, 580, 815, 874
CviQI GTAC 3 cut(s) 288, 1086, 1116
DdeI CTNAG 3 cut(s) 76, 380, 501
DpnI GATC 7 cut(s) 280, 356, 619, 833, 851, 882, 990
DpnII GATC 7 cut(s) 278, 354, 617, 831, 849, 880, 988
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 789
Eco32I GATATC 1 cut(s) 860
Eco47I GGWCC 1 cut(s) 805
Eco47III AGCGCT 1 cut(s) 104
Eco57I CTGAAG 2 cut(s) 411, 838
Eco88I CYCGRG 2 cut(s) 232, 362
EcoRII CCWGG 2 cut(s) 186, 315
EcoRV GATATC 1 cut(s) 860
EcoT14I CCWWGG 1 cut(s) 789
ErhI CCWWGG 1 cut(s) 789
Esp3I CGTCTC 1 cut(s) 519
FaeI CATG 5 cut(s) 24, 247, 583, 818, 877
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FatI CATG 5 cut(s) 20, 243, 579, 814, 873
Fnu4HI GCNGC 2 cut(s) 338, 404
FokI GGATG 3 cut(s) 18, 22, 506
Fsp4HI GCNGC 2 cut(s) 338, 404
FspBI CTAG 2 cut(s) 332, 1044
GlaI GCGC 3 cut(s) 60, 104, 336
GluI GCNGC 2 cut(s) 338, 404
HaeII RGCGCY 2 cut(s) 106, 338
HaeIII GGCC 3 cut(s) 310, 665, 788
HapII CCGG 3 cut(s) 41, 764, 1017
HgaI GACGC 1 cut(s) 42
HhaI GCGC 3 cut(s) 61, 105, 337
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 5 cut(s) 24, 247, 583, 818, 877
Hin6I GCGC 3 cut(s) 59, 103, 335
HinP1I GCGC 3 cut(s) 59, 103, 335
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 2 cut(s) 517, 898
HpaII CCGG 3 cut(s) 41, 764, 1017
HphI GGTGA 2 cut(s) 30, 950
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 8 cut(s) 79, 172, 391, 522, 650, 857, 885, 903
Hpy188III TCNNGA 6 cut(s) 67, 282, 835, 895, 977, 1152
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 7 cut(s) 35, 45, 778, 871, 898, 929, 1079
HpyCH4III ACNGT 3 cut(s) 867, 948, 997
HpyCH4IV ACGT 3 cut(s) 222, 410, 1127
HpyCH4V TGCA 6 cut(s) 347, 406, 425, 637, 821, 1131
HpyF10VI GCNNNNNNNGC 3 cut(s) 337, 376, 643
HpyF3I CTNAG 3 cut(s) 76, 380, 501
HpySE526I ACGT 3 cut(s) 222, 410, 1127
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 5 cut(s) 24, 247, 583, 818, 877
HspAI GCGC 3 cut(s) 59, 103, 335
Kzo9I GATC 7 cut(s) 278, 354, 617, 831, 849, 880, 988
LmnI GCTCC 1 cut(s) 651
Lsp1109I GCAGC 1 cut(s) 390
LweI GCATC 2 cut(s) 451, 808
MaeI CTAG 2 cut(s) 332, 1044
MaeII ACGT 3 cut(s) 222, 410, 1127
MaeIII GTNAC 3 cut(s) 691, 1096, 1123
MalI GATC 7 cut(s) 280, 356, 619, 833, 851, 882, 990
MboI GATC 7 cut(s) 278, 354, 617, 831, 849, 880, 988
MboII GAAGA 6 cut(s) 100, 145, 404, 595, 844, 898
MflI RGATCY 2 cut(s) 617, 880
MluCI AATT 3 cut(s) 198, 207, 253
MmeI TCCRAC 4 cut(s) 195, 595, 687, 1016
MnlI CCTC 9 cut(s) 85, 185, 241, 490, 622, 736, 744, 771, 787
MroXI GAANNNNTTC 2 cut(s) 289, 890
MseI TTAA 5 cut(s) 143, 252, 602, 672, 1049
MslI CAYNNNNRTG 3 cut(s) 19, 813, 1094
MspI CCGG 3 cut(s) 41, 764, 1017
MspR9I CCNGG 2 cut(s) 188, 317
MvaI CCWGG 2 cut(s) 188, 317
MvnI CGCG 2 cut(s) 53, 978
MwoI GCNNNNNNNGC 3 cut(s) 337, 376, 643
NdeII GATC 7 cut(s) 278, 354, 617, 831, 849, 880, 988
NheI GCTAGC 1 cut(s) 331
NlaIII CATG 5 cut(s) 24, 247, 583, 818, 877
NlaIV GGNNCC 4 cut(s) 15, 301, 619, 882
NmuCI GTSAC 2 cut(s) 1096, 1123
NruI TCGCGA 1 cut(s) 978
NspI RCATGY 2 cut(s) 24, 247
PaeR7I CTCGAG 1 cut(s) 232
PciI ACATGT 1 cut(s) 243
PdmI GAANNNNTTC 2 cut(s) 289, 890
PfeI GAWTC 2 cut(s) 517, 898
PflFI GACNNNGTC 1 cut(s) 727
PinAI ACCGGT 1 cut(s) 40
PkrI GCNGC 2 cut(s) 339, 405
PscI ACATGT 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 186, 315
PspGI CCWGG 2 cut(s) 186, 315
PspN4I GGNNCC 4 cut(s) 15, 301, 619, 882
PspPI GGNCC 3 cut(s) 308, 663, 805
PspXI VCTCGAGB 1 cut(s) 232
PsuI RGATCY 2 cut(s) 617, 880
PsyI GACNNNGTC 1 cut(s) 727
RruI TCGCGA 1 cut(s) 978
RsaI GTAC 3 cut(s) 289, 1087, 1117
RsaNI GTAC 3 cut(s) 288, 1086, 1116
RseI CAYNNNNRTG 3 cut(s) 19, 813, 1094
SaqAI TTAA 5 cut(s) 143, 252, 602, 672, 1049
SatI GCNGC 2 cut(s) 338, 404
Sau3AI GATC 7 cut(s) 278, 354, 617, 831, 849, 880, 988
Sau96I GGNCC 3 cut(s) 308, 663, 805
ScaI AGTACT 1 cut(s) 289
ScrFI CCNGG 2 cut(s) 188, 317
SfaNI GCATC 2 cut(s) 451, 808
SfcI CTRYAG 1 cut(s) 1101
Sfr274I CTCGAG 1 cut(s) 232
SinI GGWCC 1 cut(s) 805
SlaI CTCGAG 1 cut(s) 232
SmiMI CAYNNNNRTG 3 cut(s) 19, 813, 1094
SmlI CTYRAG 2 cut(s) 98, 232
SmoI CTYRAG 2 cut(s) 98, 232
Sse9I AATT 3 cut(s) 198, 207, 253
SsiI CCGC 2 cut(s) 323, 338
SspMI CTAG 2 cut(s) 332, 1044
StyD4I CCNGG 2 cut(s) 186, 315
StyI CCWWGG 1 cut(s) 789
TaaI ACNGT 3 cut(s) 867, 948, 997
TaiI ACGT 3 cut(s) 225, 413, 1130
TaqI TCGA 6 cut(s) 28, 68, 233, 440, 894, 968
TasI AATT 3 cut(s) 198, 207, 253
TatI WGTACW 1 cut(s) 287
TauI GCSGC 1 cut(s) 340
TfiI GAWTC 2 cut(s) 517, 898
Tru1I TTAA 5 cut(s) 143, 252, 602, 672, 1049
Tru9I TTAA 5 cut(s) 143, 252, 602, 672, 1049
TscAI CASTG 2 cut(s) 1002, 1016
TseFI GTSAC 2 cut(s) 1096, 1123
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 2 cut(s) 1096, 1123
TspDTI ATGAA 6 cut(s) 247, 282, 596, 669, 890, 1098
TspRI CASTG 2 cut(s) 1002, 1016
Tth111I GACNNNGTC 1 cut(s) 727
VpaK11BI GGWCC 1 cut(s) 805
XapI RAATTY 1 cut(s) 198
XceI RCATGY 2 cut(s) 24, 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 796
XhoI CTCGAG 1 cut(s) 232
XmnI GAANNNNTTC 2 cut(s) 289, 890
XspI CTAG 2 cut(s) 332, 1044
ZraI GACGTC 1 cut(s) 223
ZrmI AGTACT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.