Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0000777

Physical Location & Seq

Genomic Coordinates Forward (+)

16239118 .. 16240529

Gene Structure 1412 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G009670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGTCTTCATCTTCTTCTTCTTCTTCTTCTAACCTATCAAAGTCATCATCATCTACACCACCAAAGCTTCCATTGAAACCAATTCCAGGTGACTATGGCTTACCCTTCTTCGGTGCCATCAAAGATCGTTACAACTATTTCTACAACCAAGGAAAAGATGAGTTCTTCAAAACCCGAATCAGAAAGTACAACTCCACAGTCTTTCGTACCAATATGCCACCCGGCCCCTTCATCGCTTCAAACCCTAAAGTCATTGCAGTCCTCGACACCGTGAGCTTCCCTGTTCTTTTCGACACTTCCAAAGTCGAAAAACATAATGTCCTGGATGGCACTTACATGCCTTCAACATCCTTCACCAGCGGCTATCGCGTCTGCGCCTATCTCGACCCCTCAGAACCGAACCACGCTTCTCTCAAGTGCTTCTTCTTGTCTCTACTTGCCAATCGCCACCACAAGTTCATCCCACTCTTCCAAAACCACTTGTCCGACCTCTTTCTCAACCTGGAAACTGAAATTTCAAAAATTGGCAAAGCAGACTTCAACACCTCGAACAACAACATAAAGTACTTCATTGGGTTCCAAAGGGCCGACCCTGGTGGCCGTATGGGTAGCGCCGCAACTTGCACCGCAGATCACACTCGGGTGGCCAAGTTGCTGAGCTTTGCTGAAGATTTCTTGCTGCACACGTTTTCCTACCCTTCATTTTTTGTCAAATCGACCTACAAGAAGCTCTACGATGCGTTTTACGAGTCGTCAGCTTCGGTTTTGGATGAGGCTGAGAATAGTTTTGGGATTCCGAGAGACGAAGCTTGTCACAACCTGTTGTTTTTGGCTTGCTTCAATGCTTTTGGTGGCATGAAGCTTTTGTTCCCTGCTTTAATCAAGTGGGTTGGATCTGCAGGAGAGGATTTGCACCGCCAGCTCCGAAATGAAATCAGGGCTGTTGTTAAGGAAAATGATGGGAATGTTACTTTCTCGGCGTTGGATAAGATGACTTTGACCAAGTCTGTGGTTTATGAGGCTTTGAGGATTGAACCTCCGGTTCTGTTTCAGTACGGGAAGGCCAAGGAGGATATGGTGGTCCATAGCCATGATGCAGCATTTGAGATCAAGAACAGGGAAATGATCTTCGGATATCAGCCGTTCGCCATGAAGGATCCGAAGATTTTCGAGAATCCAGAGGAGTTTGTGGGGCATAGGTTTGTAGGAGAAGGTGAGAAACTGTTGAAGTATGTTTATTGGTCAAATGGTCGCGAGACTGATGATCCAACGGTGGAGAATAAGCAGTGTGCCGGGAAGGATTTGGTGTTGCTTGTGTCTAGGTTAATGTTGGTAGAGTTGTCCTTCGGTATGACACTTGTACCATTGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0006629 GO:0008150 GO:0008152 GO:0008202 GO:0016125 GO:0016491 GO:0044238 GO:0055114 GO:0071704 GO:1901360 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00592 ko01100 ko01110 map00592 map01100 map01110

Pfam Domains

Protein Families

p450

Protein Analysis

457

Amino Acids

51.5

Weight (kDa)

7.64

Isoelectric Point (pI)

33.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	304 - 412	1.1e-13	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000777)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g07410 FvH4_2g07421 FvH4_2g07423 FvH4_5g16260
malus_domestica	MD10G1085800.v1.1
prunus_persica	Prupe.8G110100_v2.0.a1
pyrus_communis	pycom10g03730 pycom10g06740
rosa_chinensis	RchiOBHm_Chr6g0260901 RchiOBHm_Chr6g0260921 RchiOBHm_Chr6g0260931 RchiOBHm_Chr6g0260961 RchiOBHm_Chr6g0261011
rosa_laevigata	RLG00000014467 RLG00000014470 RLG00000014471 RLG00000014473 RLG00000014475 RLG00000014476 RLG00000014477 RLG00000014478 RLG00000018641 RLG00000022453
rosa_multiflora	Rmu_sc0003025.1_g000005 Rmu_sc0003337.1_g000004 Rmu_sc0003337.1_g000028 Rmu_sc0005816.1_g000002 Rmu_sc0005816.1_g000003 Rmu_sc0005816.1_g000011 Rmu_sc0005816.1_g000029 Rmu_sc0005980.1_g000002 Rmu_sc0009186.1_g000002
rosa_roxburghii	Rroxscaffold_2G00122160 Rroxscaffold_7G00207490 Rroxscaffold_7G00207500 Rroxscaffold_7G00207550 Rroxscaffold_7G00207560 Rroxscaffold_7G00207620
rosa_rugosa	Rorug02G0235500.1 Rorug05G0593300 Rorug05G0593300 Rorug05G0593400 Rorug05G0593500.1 Rorug05G0594400
rosa_samantha	Rh6AG112800 Rh6AG113000 Rh6AG113100 Rh6AG113300 Rh6AG113900 Rh6BG106900 Rh6BG107000 Rh6BG107200 Rh6BG107500 Rh6CG103900 Rh6CG104000 Rh6CG104300 Rh6CG104400 Rh6CG104600 Rh6DG094800 Rh6DG094900 Rh6DG095100 Rh6DG095200 Rh6DG095400
rosa_wichuraiana	Rw0G011910 Rw6G009600 Rw6G009610 Rw6G009620 Rw6G009630 Rw6G009670 Rw6G009710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	113
AccII	CGCG	2 cut(s)	369, 1254
AciI	CCGC	4 cut(s)	360, 615, 627, 916
AclWI	GGATC	4 cut(s)	901, 1151, 1164, 1259
AcoI	YGGCCR	2 cut(s)	598, 645
AcsI	RAATTY	1 cut(s)	513
AcuI	CTGAAG	1 cut(s)	687
AfaI	GTAC	5 cut(s)	188, 208, 566, 1055, 1362
AfiI	CCNNNNNNNGG	2 cut(s)	86, 110
AflIII	ACRYGT	1 cut(s)	684
AgsI	TTSAA	9 cut(s)	76, 169, 240, 345, 519, 541, 841, 1034, 1228
AjnI	CCWGG	4 cut(s)	85, 321, 501, 592
AleI	CACNNNNGTG	1 cut(s)	641
AluBI	AGCT	8 cut(s)	67, 276, 660, 730, 758, 809, 862, 922
AluI	AGCT	8 cut(s)	67, 276, 660, 730, 758, 809, 862, 922
Alw26I	GTCTC	3 cut(s)	435, 795, 1250
AlwI	GGATC	4 cut(s)	901, 1151, 1164, 1259
Ama87I	CYCGRG	1 cut(s)	639
AoxI	GGCC	5 cut(s)	223, 585, 598, 645, 1062
ApeKI	GCWGC	2 cut(s)	679, 1097
ApoI	RAATTY	1 cut(s)	513
Asp700I	GAANNNNTTC	1 cut(s)	1166
AspLEI	GCGC	2 cut(s)	377, 614
AspS9I	GGNCC	3 cut(s)	224, 585, 1081
AsuC2I	CCSGG	2 cut(s)	222, 1294
AsuHPI	GGTGA	3 cut(s)	101, 346, 1226
AvaI	CYCGRG	1 cut(s)	639
AvaII	GGWCC	1 cut(s)	1081
BaeI	ACNNNNGTAYC	1 cut(s)	1344
BalI	TGGCCA	1 cut(s)	647
BamHI	GGATCC	1 cut(s)	1156
BanI	GGYRCC	1 cut(s)	113
BbvI	GCAGC	2 cut(s)	666, 1109
BccI	CCATC	3 cut(s)	125, 320, 953
BceAI	ACGGC	2 cut(s)	585, 1126
BciT130I	CCWGG	4 cut(s)	87, 323, 503, 594
BcnI	CCSGG	2 cut(s)	222, 1294
BcoDI	GTCTC	3 cut(s)	435, 795, 1250
BfaI	CTAG	1 cut(s)	1320
BfmI	CTRYAG	1 cut(s)	897
BfoI	RGCGCY	1 cut(s)	615
BisI	GCNGC	4 cut(s)	361, 615, 680, 1098
BlpI	GCTNAGC	1 cut(s)	656
BlsI	GCNGC	4 cut(s)	362, 616, 681, 1099
BmcAI	AGTACT	1 cut(s)	566
Bme1390I	CCNGG	6 cut(s)	87, 222, 323, 503, 594, 1294
Bme18I	GGWCC	1 cut(s)	1081
BmeT110I	CYCGRG	1 cut(s)	639
BmgT120I	GGNCC	3 cut(s)	224, 585, 1081
BmiI	GGNNCC	4 cut(s)	115, 226, 578, 1158
BmrFI	CCNGG	6 cut(s)	87, 222, 323, 503, 594, 1294
BmsI	GCATC	2 cut(s)	727, 1084
Bpu1102I	GCTNAGC	1 cut(s)	656
BpuEI	CTTGAG	1 cut(s)	398
BpuMI	CCSGG	2 cut(s)	222, 1294
BsaJI	CCNNGG	3 cut(s)	148, 592, 1065
BsaWI	WCCGGW	1 cut(s)	1039
Bsc4I	CCNNNNNNNGG	2 cut(s)	86, 110
Bse3DI	GCAATG	1 cut(s)	252
BseBI	CCWGG	4 cut(s)	87, 323, 503, 594
BseDI	CCNNGG	3 cut(s)	148, 592, 1065
BseGI	GGATG	4 cut(s)	331, 347, 459, 775
BseLI	CCNNNNNNNGG	2 cut(s)	86, 110
BseMI	GCAATG	1 cut(s)	252
BseMII	CTCAG	3 cut(s)	405, 647, 768
BseRI	GAGGAG	1 cut(s)	1196
BseXI	GCAGC	2 cut(s)	666, 1109
BsgI	GTGCAG	1 cut(s)	665
Bsh1236I	CGCG	2 cut(s)	369, 1254
BshFI	GGCC	5 cut(s)	225, 587, 600, 647, 1064
BshNI	GGYRCC	1 cut(s)	113
BsiHKCI	CYCGRG	1 cut(s)	639
BsiSI	CCGG	3 cut(s)	222, 1040, 1293
BslI	CCNNNNNNNGG	2 cut(s)	86, 110
BsmAI	GTCTC	3 cut(s)	435, 795, 1250
BsmBI	CGTCTC	1 cut(s)	795
BsnI	GGCC	5 cut(s)	225, 587, 600, 647, 1064
BsoBI	CYCGRG	1 cut(s)	639
Bsp143I	GATC	7 cut(s)	124, 631, 893, 1107, 1125, 1156, 1264
Bsp1720I	GCTNAGC	1 cut(s)	656
Bsp68I	TCGCGA	1 cut(s)	1254
BspACI	CCGC	4 cut(s)	360, 615, 627, 916
BspANI	GGCC	5 cut(s)	225, 587, 600, 647, 1064
BspCNI	CTCAG	3 cut(s)	404, 648, 769
BspFNI	CGCG	2 cut(s)	369, 1254
BspLI	GGNNCC	4 cut(s)	115, 226, 578, 1158
BspMAI	CTGCAG	1 cut(s)	901
BspPI	GGATC	4 cut(s)	901, 1151, 1164, 1259
BspT107I	GGYRCC	1 cut(s)	113
BsrDI	GCAATG	1 cut(s)	252
BssECI	CCNNGG	3 cut(s)	148, 592, 1065
BssMI	GATC	7 cut(s)	124, 631, 893, 1107, 1125, 1156, 1264
BssT1I	CCWWGG	2 cut(s)	148, 1065
Bst2UI	CCWGG	4 cut(s)	87, 323, 503, 594
Bst4CI	ACNGT	4 cut(s)	199, 271, 1224, 1273
Bst6I	CTCTTC	1 cut(s)	473
BstC8I	GCNNGC	2 cut(s)	835, 920
BstDEI	CTNAG	3 cut(s)	391, 656, 777
BstF5I	GGATG	4 cut(s)	331, 347, 459, 775
BstFNI	CGCG	2 cut(s)	369, 1254
BstH2I	RGCGCY	1 cut(s)	615
BstHHI	GCGC	2 cut(s)	377, 614
BstKTI	GATC	7 cut(s)	127, 634, 896, 1110, 1128, 1159, 1267
BstMAI	GTCTC	3 cut(s)	435, 795, 1250
BstMBI	GATC	7 cut(s)	124, 631, 893, 1107, 1125, 1156, 1264
BstMWI	GCNNNNNNNGC	2 cut(s)	366, 919
BstNI	CCWGG	4 cut(s)	87, 323, 503, 594
BstNSI	RCATGY	1 cut(s)	340
BstSCI	CCNGG	6 cut(s)	85, 220, 321, 501, 592, 1292
BstSFI	CTRYAG	1 cut(s)	897
BstUI	CGCG	2 cut(s)	369, 1254
BstV1I	GCAGC	2 cut(s)	666, 1109
BstX2I	RGATCY	2 cut(s)	893, 1156
BstXI	CCANNNNNNTGG	1 cut(s)	1009
BstYI	RGATCY	2 cut(s)	893, 1156
BsuRI	GGCC	5 cut(s)	225, 587, 600, 647, 1064
BtgZI	GCGATG	1 cut(s)	217
BtsCI	GGATG	4 cut(s)	331, 347, 459, 775
BtsI	GCAGTG	1 cut(s)	1292
BtsIMutI	CAGTG	1 cut(s)	1292
BtuMI	TCGCGA	1 cut(s)	1254
Cac8I	GCNNGC	2 cut(s)	835, 920
CfoI	GCGC	2 cut(s)	377, 614
Cfr13I	GGNCC	3 cut(s)	224, 585, 1081
CseI	GACGC	1 cut(s)	358
Csp6I	GTAC	5 cut(s)	187, 207, 565, 1054, 1361
CviAII	CATG	4 cut(s)	337, 856, 1091, 1150
CviQI	GTAC	5 cut(s)	187, 207, 565, 1054, 1361
DdeI	CTNAG	3 cut(s)	391, 656, 777
DpnI	GATC	7 cut(s)	126, 633, 895, 1109, 1127, 1158, 1266
DpnII	GATC	7 cut(s)	124, 631, 893, 1107, 1125, 1156, 1264
EaeI	YGGCCR	2 cut(s)	598, 645
Eam1104I	CTCTTC	1 cut(s)	473
EarI	CTCTTC	1 cut(s)	473
Eco130I	CCWWGG	2 cut(s)	148, 1065
Eco32I	GATATC	1 cut(s)	1136
Eco47I	GGWCC	1 cut(s)	1081
Eco57I	CTGAAG	1 cut(s)	687
Eco88I	CYCGRG	1 cut(s)	639
EcoRII	CCWGG	4 cut(s)	85, 321, 501, 592
EcoRV	GATATC	1 cut(s)	1136
EcoT14I	CCWWGG	2 cut(s)	148, 1065
ErhI	CCWWGG	2 cut(s)	148, 1065
Esp3I	CGTCTC	1 cut(s)	795
FaeI	CATG	4 cut(s)	340, 859, 1094, 1153
FalI	AAGNNNNNCTT	2 cut(s)	407, 439
FatI	CATG	4 cut(s)	336, 855, 1090, 1149
Fnu4HI	GCNGC	4 cut(s)	361, 615, 680, 1098
FokI	GGATG	4 cut(s)	334, 338, 446, 782
Fsp4HI	GCNGC	4 cut(s)	361, 615, 680, 1098
FspBI	CTAG	1 cut(s)	1320
GlaI	GCGC	2 cut(s)	376, 613
GluI	GCNGC	4 cut(s)	361, 615, 680, 1098
HaeII	RGCGCY	1 cut(s)	615
HaeIII	GGCC	5 cut(s)	225, 587, 600, 647, 1064
HapII	CCGG	3 cut(s)	222, 1040, 1293
HgaI	GACGC	1 cut(s)	358
HhaI	GCGC	2 cut(s)	377, 614
Hin1II	CATG	4 cut(s)	340, 859, 1094, 1153
Hin6I	GCGC	2 cut(s)	375, 612
HinP1I	GCGC	2 cut(s)	375, 612
HindIII	AAGCTT	3 cut(s)	65, 807, 860
HinfI	GANTC	4 cut(s)	177, 749, 793, 1174
HpaII	CCGG	3 cut(s)	222, 1040, 1293
HphI	GGTGA	3 cut(s)	101, 346, 1226
Hpy188I	TCNGA	7 cut(s)	182, 394, 487, 798, 926, 1133, 1161
Hpy188III	TCNNGA	5 cut(s)	383, 1111, 1171, 1178, 1253
HpyCH4III	ACNGT	4 cut(s)	199, 271, 1224, 1273
HpyCH4IV	ACGT	1 cut(s)	686
HpyCH4V	TGCA	6 cut(s)	257, 624, 682, 899, 913, 1097
HpyF10VI	GCNNNNNNNGC	2 cut(s)	366, 919
HpyF3I	CTNAG	3 cut(s)	391, 656, 777
HpySE526I	ACGT	1 cut(s)	686
Hsp92II	CATG	4 cut(s)	340, 859, 1094, 1153
HspAI	GCGC	2 cut(s)	375, 612
Kzo9I	GATC	7 cut(s)	124, 631, 893, 1107, 1125, 1156, 1264
LmnI	GCTCC	1 cut(s)	927
Lsp1109I	GCAGC	2 cut(s)	666, 1109
LweI	GCATC	2 cut(s)	727, 1084
MaeI	CTAG	1 cut(s)	1320
MaeII	ACGT	1 cut(s)	686
MaeIII	GTNAC	4 cut(s)	89, 128, 812, 967
MalI	GATC	7 cut(s)	126, 633, 895, 1109, 1127, 1158, 1266
MboI	GATC	7 cut(s)	124, 631, 893, 1107, 1125, 1156, 1264
MflI	RGATCY	2 cut(s)	893, 1156
MlsI	TGGCCA	1 cut(s)	647
MluCI	AATT	3 cut(s)	81, 513, 522
MluNI	TGGCCA	1 cut(s)	647
MlyI	GAGTC	1 cut(s)	758
MmeI	TCCRAC	4 cut(s)	510, 871, 963, 1292
Mox20I	TGGCCA	1 cut(s)	647
MroXI	GAANNNNTTC	1 cut(s)	1166
MscI	TGGCCA	1 cut(s)	647
MseI	TTAA	3 cut(s)	878, 948, 1325
MslI	CAYNNNNRTG	4 cut(s)	335, 641, 1089, 1369
Msp20I	TGGCCA	1 cut(s)	647
MspA1I	CMGCKG	1 cut(s)	360
MspI	CCGG	3 cut(s)	222, 1040, 1293
MspR9I	CCNGG	6 cut(s)	87, 222, 323, 503, 594, 1294
MvaI	CCWGG	4 cut(s)	87, 323, 503, 594
MvnI	CGCG	2 cut(s)	369, 1254
MwoI	GCNNNNNNNGC	2 cut(s)	366, 919
NciI	CCSGG	2 cut(s)	222, 1294
NdeII	GATC	7 cut(s)	124, 631, 893, 1107, 1125, 1156, 1264
NlaIII	CATG	4 cut(s)	340, 859, 1094, 1153
NlaIV	GGNNCC	4 cut(s)	115, 226, 578, 1158
NmeAIII	GCCGAG	1 cut(s)	956
NmuCI	GTSAC	2 cut(s)	89, 812
NruI	TCGCGA	1 cut(s)	1254
NspI	RCATGY	1 cut(s)	340
OliI	CACNNNNGTG	1 cut(s)	641
PdmI	GAANNNNTTC	1 cut(s)	1166
PfeI	GAWTC	3 cut(s)	177, 793, 1174
PflFI	GACNNNGTC	1 cut(s)	1003
PfoI	TCCNGGA	1 cut(s)	321
PkrI	GCNGC	4 cut(s)	362, 616, 681, 1099
PleI	GAGTC	1 cut(s)	757
PpsI	GAGTC	1 cut(s)	757
Psp6I	CCWGG	4 cut(s)	85, 321, 501, 592
PspGI	CCWGG	4 cut(s)	85, 321, 501, 592
PspN4I	GGNNCC	4 cut(s)	115, 226, 578, 1158
PspPI	GGNCC	3 cut(s)	224, 585, 1081
PstI	CTGCAG	1 cut(s)	901
PsuI	RGATCY	2 cut(s)	893, 1156
PsyI	GACNNNGTC	1 cut(s)	1003
RruI	TCGCGA	1 cut(s)	1254
RsaI	GTAC	5 cut(s)	188, 208, 566, 1055, 1362
RsaNI	GTAC	5 cut(s)	187, 207, 565, 1054, 1361
RseI	CAYNNNNRTG	4 cut(s)	335, 641, 1089, 1369
SaqAI	TTAA	3 cut(s)	878, 948, 1325
SatI	GCNGC	4 cut(s)	361, 615, 680, 1098
Sau3AI	GATC	7 cut(s)	124, 631, 893, 1107, 1125, 1156, 1264
Sau96I	GGNCC	3 cut(s)	224, 585, 1081
ScaI	AGTACT	1 cut(s)	566
SchI	GAGTC	1 cut(s)	758
ScrFI	CCNGG	6 cut(s)	87, 222, 323, 503, 594, 1294
SfaNI	GCATC	2 cut(s)	727, 1084
SfcI	CTRYAG	1 cut(s)	897
SinI	GGWCC	1 cut(s)	1081
SmiMI	CAYNNNNRTG	4 cut(s)	335, 641, 1089, 1369
SmlI	CTYRAG	1 cut(s)	413
SmoI	CTYRAG	1 cut(s)	413
Sse9I	AATT	3 cut(s)	81, 513, 522
SsiI	CCGC	4 cut(s)	360, 615, 627, 916
SspMI	CTAG	1 cut(s)	1320
StyD4I	CCNGG	6 cut(s)	85, 220, 321, 501, 592, 1292
StyI	CCWWGG	2 cut(s)	148, 1065
TaaI	ACNGT	4 cut(s)	199, 271, 1224, 1273
TaiI	ACGT	1 cut(s)	689
TaqI	TCGA	7 cut(s)	264, 291, 306, 384, 548, 716, 1170
TasI	AATT	3 cut(s)	81, 513, 522
TatI	WGTACW	2 cut(s)	186, 564
TauI	GCSGC	2 cut(s)	363, 617
TfiI	GAWTC	3 cut(s)	177, 793, 1174
Tru1I	TTAA	3 cut(s)	878, 948, 1325
Tru9I	TTAA	3 cut(s)	878, 948, 1325
TscAI	CASTG	1 cut(s)	1292
TseFI	GTSAC	2 cut(s)	89, 812
TseI	GCWGC	2 cut(s)	679, 1097
Tsp45I	GTSAC	2 cut(s)	89, 812
TspDTI	ATGAA	7 cut(s)	220, 448, 559, 690, 872, 945, 1166
TspRI	CASTG	1 cut(s)	1292
Tth111I	GACNNNGTC	1 cut(s)	1003
VpaK11BI	GGWCC	1 cut(s)	1081
XapI	RAATTY	1 cut(s)	513
XceI	RCATGY	1 cut(s)	340
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1072
XmnI	GAANNNNTTC	1 cut(s)	1166
XspI	CTAG	1 cut(s)	1320
ZrmI	AGTACT	1 cut(s)	566

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.