RLG00000014612

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003936
Physical Location & Seq
Forward (+)
55445165 .. 55446076
912 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014612

Sequence Viewer

Length: 912 bp
ATGGCCGCCGCCGCCGATCACTCCGACGTCGAACTCGAGTTCCGCTTCTTCCGCGTCCACAAAGACGGCACCATCCACCGAACCCATCCCCACATTGACAAAGTCCCACCCTCCGACGACCCGACCACCGAGATCCGATCCAAAGACGTCACCATCTCAACCGACCCACCCATCTCCGCCCGCATTTTCCTACCCAACTCCGTCGACCCTACCCGGAAGCTCCCCCTCCTCCTCTACCTCCACGGCGGCGGCTTCTGCTTCGAGTCGCCCTGCTCCTCCACCTACCACAACTACGTCTCCACCGTCGCCGCTTCCGCCAAAGTCATCGCCTTCTCAGTCGAGTACGGTCTCTTCCCGACCCGGCCCATTCCCGCTTGCTACGACGACTCCTGGGCCGCTCTCCAATGGCTCGCGACCCATGCAGACAAAACCGGGTCGGAGCCGTGGCTAAACGACTACGCCGACTTGACCCGCGTCTTCGTCGGCGGGGATAGCGCTGGAGGGAACATAGCGCACAATTTGGTGTCCCGGGTTGGGTCGATCGGGTTACCCGGAGCGAAAGTGGTCGGGGCAATACTGGTGCATCCCTACTTTGGTGGGACTGATGATGATAAGATGTGGCTGTACATGAATAAGGAGAATGAAGGGTTGCAGGACCGGAGGCTGGTACCGGCCGCAGAGGATCTACGGCGGCTTGGGTGTGAGAGGGTGTTGGTGTTTGTGGCGGAGATGGACCATTTGAAGGGTGGCGGGAAGAGGTATGTGGAGGAGCTGAAGAAGAGTGGGTGGAGTGGGAGTGTGGAGGTGAAGGAGACTGTAGGGAAAGACCATTGCTTTCACTTGGCTGAACCCAAGGATGAGCAGACTATGGATTTGGTCAAGAAAATTGCTTCTTTTATTAATCTACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0009056 GO:0016787 GO:0016788 GO:0044424 GO:0044444 GO:0044464 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

304

Amino Acids

33.49

Weight (kDa)

5.95

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 63 - 171 8.9e-09 BD-FAE
Abhydrolase_3 PF07859 77 - 280 1.7e-38 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 30, 150
Acc65I GGTACC 1 cut(s) 667
AccB1I GGYRCC 2 cut(s) 68, 667
AccBSI CCGCTC 1 cut(s) 398
AccI GTMKAC 1 cut(s) 204
AccII CGCG 3 cut(s) 54, 413, 474
AclWI GGATC 3 cut(s) 127, 132, 690
AcoI YGGCCR 2 cut(s) 3, 672
AcuI CTGAAG 1 cut(s) 794
AcyI GRCGYC 2 cut(s) 27, 147
AfaI GTAC 3 cut(s) 344, 626, 669
AfeI AGCGCT 1 cut(s) 496
AfiI CCNNNNNNNGG 4 cut(s) 534, 593, 664, 742
AgsI TTSAA 1 cut(s) 742
AjnI CCWGG 1 cut(s) 389
AloI GAACNNNNNNTCC 2 cut(s) 24, 56
AluBI AGCT 2 cut(s) 220, 772
AluI AGCT 2 cut(s) 220, 772
Alw26I GTCTC 3 cut(s) 301, 353, 806
AlwI GGATC 3 cut(s) 127, 132, 690
Ama87I CYCGRG 2 cut(s) 35, 528
Aor51HI AGCGCT 1 cut(s) 496
AoxI GGCC 4 cut(s) 3, 362, 393, 672
ArsI GACNNNNNNTTYG 2 cut(s) 856, 888
AseI ATTAAT 1 cut(s) 900
Asp718I GGTACC 1 cut(s) 667
AspLEI GCGC 2 cut(s) 497, 514
AspS9I GGNCC 4 cut(s) 363, 393, 655, 733
AsuC2I CCSGG 6 cut(s) 214, 361, 433, 529, 530, 552
AsuHPI GGTGA 2 cut(s) 142, 817
AvaI CYCGRG 2 cut(s) 35, 528
AvaII GGWCC 2 cut(s) 655, 733
BanI GGYRCC 2 cut(s) 68, 667
BarI GAAGNNNNNNTAC 2 cut(s) 335, 367
BbsI GAAGAC 1 cut(s) 469
BccI CCATC 5 cut(s) 80, 93, 161, 179, 724
BceAI ACGGC 4 cut(s) 82, 259, 427, 704
BciT130I CCWGG 1 cut(s) 391
BcnI CCSGG 6 cut(s) 214, 361, 433, 529, 530, 552
BcoDI GTCTC 3 cut(s) 301, 353, 806
BfaI CTAG 1 cut(s) 910
BfmI CTRYAG 1 cut(s) 816
BfoI RGCGCY 1 cut(s) 498
BisI GCNGC 9 cut(s) 6, 9, 12, 247, 250, 309, 396, 675, 692
BlsI GCNGC 9 cut(s) 7, 10, 13, 248, 251, 310, 397, 676, 693
Bme1390I CCNGG 7 cut(s) 214, 361, 391, 433, 529, 530, 552
Bme18I GGWCC 2 cut(s) 655, 733
BmeT110I CYCGRG 2 cut(s) 35, 528
BmgT120I GGNCC 4 cut(s) 363, 393, 655, 733
BmiI GGNNCC 3 cut(s) 70, 441, 669
BmrFI CCNGG 7 cut(s) 214, 361, 391, 433, 529, 530, 552
BmsI GCATC 1 cut(s) 592
BoxI GACNNNNGTC 1 cut(s) 473
BpiI GAAGAC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 519
BpuMI CCSGG 6 cut(s) 214, 361, 433, 529, 530, 552
BsaHI GRCGYC 2 cut(s) 27, 147
BsaI GGTCTC 1 cut(s) 353
BsaJI CCNNGG 5 cut(s) 241, 390, 443, 528, 852
BsaWI WCCGGW 1 cut(s) 657
BsaXI ACNNNNNCTCC 8 cut(s) 281, 311, 371, 401, 546, 576, 781, 811
Bsc4I CCNNNNNNNGG 4 cut(s) 534, 593, 664, 742
Bse118I RCCGGY 1 cut(s) 670
Bse1I ACTGG 1 cut(s) 582
Bse3DI GCAATG 1 cut(s) 829
BseBI CCWGG 1 cut(s) 391
BseDI CCNNGG 5 cut(s) 241, 390, 443, 528, 852
BseGI GGATG 4 cut(s) 72, 85, 583, 862
BseLI CCNNNNNNNGG 4 cut(s) 534, 593, 664, 742
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 1 cut(s) 582
BseRI GAGGAG 4 cut(s) 218, 221, 265, 782
BseX3I CGGCCG 1 cut(s) 672
Bsh1236I CGCG 3 cut(s) 54, 413, 474
Bsh1285I CGRYCG 2 cut(s) 543, 675
BshFI GGCC 4 cut(s) 5, 364, 395, 674
BshNI GGYRCC 2 cut(s) 68, 667
BsiEI CGRYCG 2 cut(s) 543, 675
BsiHKCI CYCGRG 2 cut(s) 35, 528
BsiSI CCGG 7 cut(s) 214, 361, 432, 529, 552, 658, 671
BslFI GGGAC 3 cut(s) 89, 511, 613
BslI CCNNNNNNNGG 4 cut(s) 534, 593, 664, 742
BsmAI GTCTC 3 cut(s) 301, 353, 806
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 3 cut(s) 89, 511, 613
BsnI GGCC 4 cut(s) 5, 364, 395, 674
Bso31I GGTCTC 1 cut(s) 353
BsoBI CYCGRG 2 cut(s) 35, 528
Bsp1407I TGTACA 1 cut(s) 624
Bsp143I GATC 5 cut(s) 16, 132, 137, 540, 682
Bsp68I TCGCGA 1 cut(s) 413
BspANI GGCC 4 cut(s) 5, 364, 395, 674
BspCNI CTCAG 1 cut(s) 347
BspFNI CGCG 3 cut(s) 54, 413, 474
BspLI GGNNCC 3 cut(s) 70, 441, 669
BspPI GGATC 3 cut(s) 127, 132, 690
BspT107I GGYRCC 2 cut(s) 68, 667
BspTNI GGTCTC 1 cut(s) 353
BsrBI CCGCTC 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 829
BsrFI RCCGGY 1 cut(s) 670
BsrGI TGTACA 1 cut(s) 624
BsrI ACTGG 1 cut(s) 582
BssAI RCCGGY 1 cut(s) 670
BssECI CCNNGG 5 cut(s) 241, 390, 443, 528, 852
BssMI GATC 5 cut(s) 16, 132, 137, 540, 682
BssNI GRCGYC 2 cut(s) 27, 147
BssT1I CCWWGG 1 cut(s) 852
Bst2UI CCWGG 1 cut(s) 391
Bst4CI ACNGT 3 cut(s) 304, 347, 817
Bst6I CTCTTC 3 cut(s) 356, 749, 773
BstACI GRCGYC 2 cut(s) 27, 147
BstAUI TGTACA 1 cut(s) 624
BstC8I GCNNGC 3 cut(s) 181, 376, 411
BstDEI CTNAG 1 cut(s) 334
BstDSI CCRYGG 2 cut(s) 241, 443
BstEII GGTNACC 1 cut(s) 546
BstF5I GGATG 4 cut(s) 72, 85, 583, 862
BstFNI CGCG 3 cut(s) 54, 413, 474
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 2 cut(s) 497, 514
BstKTI GATC 5 cut(s) 19, 135, 140, 543, 685
BstMAI GTCTC 3 cut(s) 301, 353, 806
BstMBI GATC 5 cut(s) 16, 132, 137, 540, 682
BstMCI CGRYCG 2 cut(s) 543, 675
BstMWI GCNNNNNNNGC 6 cut(s) 11, 51, 255, 314, 419, 492
BstNI CCWGG 1 cut(s) 391
BstPAI GACNNNNGTC 1 cut(s) 473
BstPI GGTNACC 1 cut(s) 546
BstSCI CCNGG 7 cut(s) 212, 359, 389, 431, 527, 528, 550
BstSFI CTRYAG 1 cut(s) 816
BstUI CGCG 3 cut(s) 54, 413, 474
BstV2I GAAGAC 1 cut(s) 469
BstX2I RGATCY 2 cut(s) 132, 682
BstYI RGATCY 2 cut(s) 132, 682
BstZI CGGCCG 1 cut(s) 672
BsuRI GGCC 4 cut(s) 5, 364, 395, 674
BtgI CCRYGG 2 cut(s) 241, 443
BtgZI GCGATG 1 cut(s) 310
BtsCI GGATG 4 cut(s) 72, 85, 583, 862
BtuMI TCGCGA 1 cut(s) 413
Cac8I GCNNGC 3 cut(s) 181, 376, 411
CfoI GCGC 2 cut(s) 497, 514
Cfr10I RCCGGY 1 cut(s) 670
Cfr13I GGNCC 4 cut(s) 363, 393, 655, 733
Cfr9I CCCGGG 1 cut(s) 528
CseI GACGC 2 cut(s) 43, 463
Csp6I GTAC 3 cut(s) 343, 625, 668
CviAII CATG 2 cut(s) 419, 628
CviQI GTAC 3 cut(s) 343, 625, 668
DdeI CTNAG 1 cut(s) 334
DpnI GATC 5 cut(s) 18, 134, 139, 542, 684
DpnII GATC 5 cut(s) 16, 132, 137, 540, 682
EaeI YGGCCR 2 cut(s) 3, 672
EagI CGGCCG 1 cut(s) 672
Eam1104I CTCTTC 3 cut(s) 356, 749, 773
EarI CTCTTC 3 cut(s) 356, 749, 773
EciI GGCGGA 3 cut(s) 166, 304, 740
EclXI CGGCCG 1 cut(s) 672
Eco130I CCWWGG 1 cut(s) 852
Eco31I GGTCTC 1 cut(s) 353
Eco47I GGWCC 2 cut(s) 655, 733
Eco47III AGCGCT 1 cut(s) 496
Eco52I CGGCCG 1 cut(s) 672
Eco57I CTGAAG 1 cut(s) 794
Eco88I CYCGRG 2 cut(s) 35, 528
Eco91I GGTNACC 1 cut(s) 546
EcoO65I GGTNACC 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 389
EcoT14I CCWWGG 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 852
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 2 cut(s) 422, 631
FaiI YATR 5 cut(s) 420, 509, 629, 762, 869
FaqI GGGAC 3 cut(s) 89, 511, 613
FatI CATG 2 cut(s) 418, 627
FauI CCCGC 5 cut(s) 188, 379, 479, 479, 743
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 9 cut(s) 6, 9, 12, 247, 250, 309, 396, 675, 692
FokI GGATG 4 cut(s) 59, 72, 570, 869
Fsp4HI GCNGC 9 cut(s) 6, 9, 12, 247, 250, 309, 396, 675, 692
FspBI CTAG 1 cut(s) 910
GlaI GCGC 2 cut(s) 496, 513
GluI GCNGC 9 cut(s) 6, 9, 12, 247, 250, 309, 396, 675, 692
GsuI CTGGAG 1 cut(s) 519
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 4 cut(s) 5, 364, 395, 674
HapII CCGG 7 cut(s) 214, 361, 432, 529, 552, 658, 671
HgaI GACGC 2 cut(s) 43, 463
HhaI GCGC 2 cut(s) 497, 514
Hin1I GRCGYC 2 cut(s) 27, 147
Hin1II CATG 2 cut(s) 422, 631
Hin6I GCGC 2 cut(s) 495, 512
HinP1I GCGC 2 cut(s) 495, 512
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 2 cut(s) 263, 386
HpaII CCGG 7 cut(s) 214, 361, 432, 529, 552, 658, 671
HphI GGTGA 2 cut(s) 142, 817
Hpy166II GTNNAC 2 cut(s) 58, 205
Hpy188I TCNGA 4 cut(s) 25, 115, 137, 439
Hpy188III TCNNGA 3 cut(s) 355, 412, 880
Hpy8I GTNNAC 2 cut(s) 58, 205
Hpy99I CGWCG 7 cut(s) 29, 32, 119, 206, 308, 386, 485
HpyAV CCTTC 4 cut(s) 340, 638, 736, 802
HpyCH4III ACNGT 3 cut(s) 304, 347, 817
HpyCH4IV ACGT 3 cut(s) 27, 147, 294
HpyCH4V TGCA 3 cut(s) 422, 583, 652
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 51, 255, 314, 419, 492
HpyF3I CTNAG 1 cut(s) 334
HpySE526I ACGT 3 cut(s) 27, 147, 294
Hsp92I GRCGYC 2 cut(s) 27, 147
Hsp92II CATG 2 cut(s) 422, 631
HspAI GCGC 2 cut(s) 495, 512
KpnI GGTACC 1 cut(s) 671
Kzo9I GATC 5 cut(s) 16, 132, 137, 540, 682
LmnI GCTCC 5 cut(s) 225, 278, 439, 554, 769
LweI GCATC 1 cut(s) 592
MaeI CTAG 1 cut(s) 910
MaeII ACGT 3 cut(s) 27, 147, 294
MaeIII GTNAC 2 cut(s) 148, 546
MalI GATC 5 cut(s) 18, 134, 139, 542, 684
MbiI CCGCTC 1 cut(s) 398
MboI GATC 5 cut(s) 16, 132, 137, 540, 682
MboII GAAGA 6 cut(s) 40, 343, 469, 766, 787, 790
MflI RGATCY 2 cut(s) 132, 682
MluCI AATT 2 cut(s) 517, 885
MlyI GAGTC 2 cut(s) 272, 380
MmeI TCCRAC 3 cut(s) 48, 138, 417
MseI TTAA 1 cut(s) 900
MspI CCGG 7 cut(s) 214, 361, 432, 529, 552, 658, 671
MspR9I CCNGG 7 cut(s) 214, 361, 391, 433, 529, 530, 552
MvaI CCWGG 1 cut(s) 391
MvnI CGCG 3 cut(s) 54, 413, 474
MwoI GCNNNNNNNGC 6 cut(s) 11, 51, 255, 314, 419, 492
NciI CCSGG 6 cut(s) 214, 361, 433, 529, 530, 552
NdeII GATC 5 cut(s) 16, 132, 137, 540, 682
NlaIII CATG 2 cut(s) 422, 631
NlaIV GGNNCC 3 cut(s) 70, 441, 669
NmuCI GTSAC 1 cut(s) 148
NruI TCGCGA 1 cut(s) 413
PaeR7I CTCGAG 1 cut(s) 35
PcsI WCGNNNNNNNCGW 3 cut(s) 33, 300, 459
PflFI GACNNNGTC 1 cut(s) 101
PkrI GCNGC 9 cut(s) 7, 10, 13, 248, 251, 310, 397, 676, 693
Ple19I CGATCG 1 cut(s) 543
PleI GAGTC 2 cut(s) 271, 380
PpsI GAGTC 2 cut(s) 271, 380
PshAI GACNNNNGTC 1 cut(s) 473
PshBI ATTAAT 1 cut(s) 900
Psp6I CCWGG 1 cut(s) 389
PspEI GGTNACC 1 cut(s) 546
PspGI CCWGG 1 cut(s) 389
PspN4I GGNNCC 3 cut(s) 70, 441, 669
PspPI GGNCC 4 cut(s) 363, 393, 655, 733
PspXI VCTCGAGB 1 cut(s) 35
PsuI RGATCY 2 cut(s) 132, 682
PsyI GACNNNGTC 1 cut(s) 101
PvuI CGATCG 1 cut(s) 543
RruI TCGCGA 1 cut(s) 413
RsaI GTAC 3 cut(s) 344, 626, 669
RsaNI GTAC 3 cut(s) 343, 625, 668
SalI GTCGAC 1 cut(s) 203
SaqAI TTAA 1 cut(s) 900
SatI GCNGC 9 cut(s) 6, 9, 12, 247, 250, 309, 396, 675, 692
Sau3AI GATC 5 cut(s) 16, 132, 137, 540, 682
Sau96I GGNCC 4 cut(s) 363, 393, 655, 733
SchI GAGTC 2 cut(s) 272, 380
ScrFI CCNGG 7 cut(s) 214, 361, 391, 433, 529, 530, 552
SetI ASST 9 cut(s) 30, 150, 222, 240, 284, 297, 761, 774, 807
SfaNI GCATC 1 cut(s) 592
SfcI CTRYAG 1 cut(s) 816
Sfr274I CTCGAG 1 cut(s) 35
SinI GGWCC 2 cut(s) 655, 733
SlaI CTCGAG 1 cut(s) 35
SmaI CCCGGG 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 2 cut(s) 517, 885
SspMI CTAG 1 cut(s) 910
StyD4I CCNGG 7 cut(s) 212, 359, 389, 431, 527, 528, 550
StyI CCWWGG 1 cut(s) 852
TaaI ACNGT 3 cut(s) 304, 347, 817
TaiI ACGT 3 cut(s) 30, 150, 297
TaqI TCGA 6 cut(s) 30, 36, 204, 261, 339, 539
TasI AATT 2 cut(s) 517, 885
TatI WGTACW 1 cut(s) 624
TauI GCSGC 9 cut(s) 8, 11, 14, 249, 252, 311, 398, 677, 694
Tru1I TTAA 1 cut(s) 900
Tru9I TTAA 1 cut(s) 900
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 644, 657
TspGWI ACGGA 1 cut(s) 190
TspMI CCCGGG 1 cut(s) 528
Tth111I GACNNNGTC 1 cut(s) 101
VpaK11BI GGWCC 2 cut(s) 655, 733
VspI ATTAAT 1 cut(s) 900
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XhoI CTCGAG 1 cut(s) 35
XmaI CCCGGG 1 cut(s) 528
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 910
ZraI GACGTC 2 cut(s) 28, 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.