RLG00000020618

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008829
Physical Location & Seq
Reverse (-)
66758895 .. 66759915
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020618

Sequence Viewer

Length: 894 bp
ATGAAGAAGTGGCTGGCTCACATCAAATATGGGTTTACAGAATTGGTGAAGATACAGGTAGAAGAAGGAGACGATCTAGATGATGACAATGAGACGGTTCTGAAGGACGTTCTCTTTGGTGAGTCGAGAGTCGTATTCGGATTCGATCAGGAGGCAGAGACGATTGAGCGCTTGCTCTTTGGTGAGTCCAGAACTGGATTTAGGTGTGTTGGAGTCGTCGGCATTGCTGGTGTGGGCAAGACGGCACTTGTGGAAAAGGAAGAAACAAATATAAAAAAAGAGGACGCAGATCTTATTGGTTTCAGGATTTTGACGTTCATTCTTGACAAGTTGGGCCATGATGACAATGATGACAAAGCCAACCAGACGCTGAGTTCCCTCTTGCAAAAAATGAAGCAGCTCTTGTTGGGTAAACGTTATCTGATTGTGTTGGATGGTGTTTGGCATATGAACGCCTTCTATTGCTCTTTAGGGAATAACAATGTCAACGGCATGGATCGCCTATCGGATGGATTGCCTTGGGATAGCGGCGGTGCGGTGATCGTCACTAGTAGGAGGCTGGAAGTGGCTCAACAAATGGGGGGCGCAGATAACTTGATTATTCCTGGTGTCTATGATTATACTAATGATATTAAAGACAATAATCATGATTTTGATCCTAGTAGAGAGGAAAACAATAATCATGATTTTGATCCTAGTAGAGAGGAAAAGCTAAGCGAACTAGCTCCTGAAAAAGCTTTGAATAGGATCAAGGATCAAACTGAGGAGGAATTTCAGGGTCTACCATTGGCTGCTAAGGTACTCGCAACCGCCATTCCTGGACACATTCGTACAAGAGATGCAGGAAGGTTCCCACGAAACTCGAGTTTGCTTAATCTTGTTTGTTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

298

Amino Acids

33.29

Weight (kDa)

4.85

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 53 - 196 2.3e-11 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 781
AciI CCGC 4 cut(s) 528, 531, 536, 810
AclI AACGTT 1 cut(s) 415
AclWI GGATC 5 cut(s) 504, 650, 686, 755, 762
AcsI RAATTY 1 cut(s) 770
AcuI CTGAAG 1 cut(s) 122
AfaI GTAC 2 cut(s) 801, 832
AfeI AGCGCT 1 cut(s) 170
AgsI TTSAA 1 cut(s) 742
AhlI ACTAGT 1 cut(s) 548
AjnI CCWGG 2 cut(s) 604, 817
AluBI AGCT 4 cut(s) 400, 712, 725, 737
AluI AGCT 4 cut(s) 400, 712, 725, 737
Alw26I GTCTC 3 cut(s) 63, 86, 152
AlwI GGATC 5 cut(s) 504, 650, 686, 755, 762
AlwNI CAGNNNCTG 1 cut(s) 370
Ama87I CYCGRG 1 cut(s) 862
Aor51HI AGCGCT 1 cut(s) 170
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 2 cut(s) 397, 791
ApoI RAATTY 1 cut(s) 770
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
Asp700I GAANNNNTTC 1 cut(s) 455
AspLEI GCGC 2 cut(s) 171, 587
AspS9I GGNCC 1 cut(s) 334
AsuHPI GGTGA 4 cut(s) 58, 131, 194, 550
AvaI CYCGRG 1 cut(s) 862
BbvI GCAGC 2 cut(s) 409, 778
BccI CCATC 2 cut(s) 428, 503
BceAI ACGGC 2 cut(s) 258, 505
BciT130I CCWGG 2 cut(s) 606, 819
BcoDI GTCTC 3 cut(s) 63, 86, 152
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 5 cut(s) 77, 549, 660, 696, 722
BfoI RGCGCY 1 cut(s) 172
BglII AGATCT 1 cut(s) 289
BisI GCNGC 3 cut(s) 398, 529, 792
BlpI GCTNAGC 1 cut(s) 713
BlsI GCNGC 3 cut(s) 399, 530, 793
Bme1390I CCNGG 2 cut(s) 606, 819
BmeT110I CYCGRG 1 cut(s) 862
BmgT120I GGNCC 1 cut(s) 334
BmiI GGNNCC 1 cut(s) 851
BmrFI CCNGG 2 cut(s) 606, 819
BmsI GCATC 1 cut(s) 829
Bpu10I CCTNAGC 1 cut(s) 795
Bpu1102I GCTNAGC 1 cut(s) 713
BsaBI GATNNNNATC 2 cut(s) 654, 690
BsaJI CCNNGG 1 cut(s) 518
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 222
Bse8I GATNNNNATC 2 cut(s) 654, 690
BseBI CCWGG 2 cut(s) 606, 819
BseDI CCNNGG 1 cut(s) 518
BseGI GGATG 2 cut(s) 439, 514
BseJI GATNNNNATC 2 cut(s) 654, 690
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 2 cut(s) 362, 753
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 779
BseXI GCAGC 2 cut(s) 409, 778
BshFI GGCC 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 862
BsmAI GTCTC 3 cut(s) 63, 86, 152
BsmBI CGTCTC 3 cut(s) 63, 86, 152
BsnI GGCC 1 cut(s) 336
BsoBI CYCGRG 1 cut(s) 862
Bsp143I GATC 9 cut(s) 73, 145, 289, 496, 540, 655, 691, 747, 754
Bsp1720I GCTNAGC 1 cut(s) 713
BspACI CCGC 4 cut(s) 528, 531, 536, 810
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 2 cut(s) 363, 754
BspHI TCATGA 2 cut(s) 646, 682
BspLI GGNNCC 1 cut(s) 851
BspPI GGATC 5 cut(s) 504, 650, 686, 755, 762
BsrDI GCAATG 1 cut(s) 222
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 518
BssMI GATC 9 cut(s) 73, 145, 289, 496, 540, 655, 691, 747, 754
BssT1I CCWWGG 1 cut(s) 518
Bst2UI CCWGG 2 cut(s) 606, 819
Bst4CI ACNGT 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 15, 173
BstDEI CTNAG 4 cut(s) 371, 713, 762, 795
BstF5I GGATG 2 cut(s) 439, 514
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 2 cut(s) 171, 587
BstKTI GATC 9 cut(s) 76, 148, 292, 499, 543, 658, 694, 750, 757
BstMAI GTCTC 3 cut(s) 63, 86, 152
BstMBI GATC 9 cut(s) 73, 145, 289, 496, 540, 655, 691, 747, 754
BstMWI GCNNNNNNNGC 1 cut(s) 498
BstNI CCWGG 2 cut(s) 606, 819
BstSCI CCNGG 2 cut(s) 604, 817
BstV1I GCAGC 2 cut(s) 409, 778
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BsuRI GGCC 1 cut(s) 336
BtsCI GGATG 2 cut(s) 439, 514
Cac8I GCNNGC 2 cut(s) 15, 173
CaiI CAGNNNCTG 1 cut(s) 370
CciI TCATGA 2 cut(s) 646, 682
CfoI GCGC 2 cut(s) 171, 587
Cfr13I GGNCC 1 cut(s) 334
CseI GACGC 2 cut(s) 293, 376
Csp6I GTAC 2 cut(s) 800, 831
CviAII CATG 4 cut(s) 338, 493, 647, 683
CviQI GTAC 2 cut(s) 800, 831
DdeI CTNAG 4 cut(s) 371, 713, 762, 795
DpnI GATC 9 cut(s) 75, 147, 291, 498, 542, 657, 693, 749, 756
DpnII GATC 9 cut(s) 73, 145, 289, 496, 540, 655, 691, 747, 754
Eco130I CCWWGG 1 cut(s) 518
Eco47III AGCGCT 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 862
EcoRII CCWGG 2 cut(s) 604, 817
EcoT14I CCWWGG 1 cut(s) 518
ErhI CCWWGG 1 cut(s) 518
Esp3I CGTCTC 3 cut(s) 63, 86, 152
FaeI CATG 4 cut(s) 341, 496, 650, 686
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FatI CATG 4 cut(s) 337, 492, 646, 682
FauNDI CATATG 1 cut(s) 447
FblI GTMKAC 1 cut(s) 781
Fnu4HI GCNGC 3 cut(s) 398, 529, 792
FokI GGATG 2 cut(s) 446, 521
Fsp4HI GCNGC 3 cut(s) 398, 529, 792
FspBI CTAG 5 cut(s) 77, 549, 660, 696, 722
GlaI GCGC 2 cut(s) 170, 586
GluI GCNGC 3 cut(s) 398, 529, 792
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 1 cut(s) 336
HgaI GACGC 2 cut(s) 293, 376
HhaI GCGC 2 cut(s) 171, 587
Hin1II CATG 4 cut(s) 341, 496, 650, 686
Hin6I GCGC 2 cut(s) 169, 585
HinP1I GCGC 2 cut(s) 169, 585
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 5 cut(s) 122, 129, 141, 185, 213
HphI GGTGA 4 cut(s) 58, 131, 194, 550
Hpy166II GTNNAC 4 cut(s) 36, 413, 487, 782
Hpy188I TCNGA 4 cut(s) 102, 140, 423, 508
Hpy188III TCNNGA 9 cut(s) 77, 126, 149, 189, 304, 323, 647, 683, 728
Hpy8I GTNNAC 4 cut(s) 36, 413, 487, 782
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 4 cut(s) 59, 97, 466, 840
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 3 cut(s) 108, 314, 415
HpyCH4V TGCA 2 cut(s) 385, 842
HpyF10VI GCNNNNNNNGC 1 cut(s) 498
HpyF3I CTNAG 4 cut(s) 371, 713, 762, 795
HpySE526I ACGT 3 cut(s) 108, 314, 415
Hsp92II CATG 4 cut(s) 341, 496, 650, 686
HspAI GCGC 2 cut(s) 169, 585
Kzo9I GATC 9 cut(s) 73, 145, 289, 496, 540, 655, 691, 747, 754
LmnI GCTCC 1 cut(s) 730
Lsp1109I GCAGC 2 cut(s) 409, 778
LweI GCATC 1 cut(s) 829
MaeI CTAG 5 cut(s) 77, 549, 660, 696, 722
MaeII ACGT 3 cut(s) 108, 314, 415
MaeIII GTNAC 1 cut(s) 544
MalI GATC 9 cut(s) 75, 147, 291, 498, 542, 657, 693, 749, 756
MboI GATC 9 cut(s) 73, 145, 289, 496, 540, 655, 691, 747, 754
MboII GAAGA 4 cut(s) 16, 61, 74, 272
MflI RGATCY 1 cut(s) 289
MluCI AATT 2 cut(s) 41, 770
MlyI GAGTC 4 cut(s) 131, 138, 194, 222
MmeI TCCRAC 2 cut(s) 190, 411
MnlI CCTC 8 cut(s) 145, 274, 389, 549, 661, 697, 757, 760
MroXI GAANNNNTTC 1 cut(s) 455
MseI TTAA 2 cut(s) 633, 873
MspR9I CCNGG 2 cut(s) 606, 819
MvaI CCWGG 2 cut(s) 606, 819
MwoI GCNNNNNNNGC 1 cut(s) 498
NdeI CATATG 1 cut(s) 447
NdeII GATC 9 cut(s) 73, 145, 289, 496, 540, 655, 691, 747, 754
NlaIII CATG 4 cut(s) 341, 496, 650, 686
NlaIV GGNNCC 1 cut(s) 851
NmuCI GTSAC 1 cut(s) 544
PaeR7I CTCGAG 1 cut(s) 862
PagI TCATGA 2 cut(s) 646, 682
PdmI GAANNNNTTC 1 cut(s) 455
PfeI GAWTC 1 cut(s) 141
PfoI TCCNGGA 1 cut(s) 817
PkrI GCNGC 3 cut(s) 399, 530, 793
PleI GAGTC 4 cut(s) 130, 137, 193, 221
PpsI GAGTC 4 cut(s) 130, 137, 193, 221
Psp1406I AACGTT 1 cut(s) 415
Psp6I CCWGG 2 cut(s) 604, 817
PspGI CCWGG 2 cut(s) 604, 817
PspN4I GGNNCC 1 cut(s) 851
PspPI GGNCC 1 cut(s) 334
PspXI VCTCGAGB 1 cut(s) 862
PstNI CAGNNNCTG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 289
RsaI GTAC 2 cut(s) 801, 832
RsaNI GTAC 2 cut(s) 800, 831
SaqAI TTAA 2 cut(s) 633, 873
SatI GCNGC 3 cut(s) 398, 529, 792
Sau3AI GATC 9 cut(s) 73, 145, 289, 496, 540, 655, 691, 747, 754
Sau96I GGNCC 1 cut(s) 334
SchI GAGTC 4 cut(s) 131, 138, 194, 222
ScrFI CCNGG 2 cut(s) 606, 819
SfaNI GCATC 1 cut(s) 829
Sfr274I CTCGAG 1 cut(s) 862
SlaI CTCGAG 1 cut(s) 862
SmlI CTYRAG 1 cut(s) 862
SmoI CTYRAG 1 cut(s) 862
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 2 cut(s) 41, 770
SsiI CCGC 4 cut(s) 528, 531, 536, 810
SspMI CTAG 5 cut(s) 77, 549, 660, 696, 722
StyD4I CCNGG 2 cut(s) 604, 817
StyI CCWWGG 1 cut(s) 518
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 3 cut(s) 111, 317, 418
TaqI TCGA 3 cut(s) 125, 144, 863
TasI AATT 2 cut(s) 41, 770
TauI GCSGC 1 cut(s) 531
TfiI GAWTC 1 cut(s) 141
Tru1I TTAA 2 cut(s) 633, 873
Tru9I TTAA 2 cut(s) 633, 873
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 2 cut(s) 397, 791
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 4 cut(s) 17, 307, 407, 464
XapI RAATTY 1 cut(s) 770
XbaI TCTAGA 1 cut(s) 76
XhoI CTCGAG 1 cut(s) 862
XmiI GTMKAC 1 cut(s) 781
XmnI GAANNNNTTC 1 cut(s) 455
XspI CTAG 5 cut(s) 77, 549, 660, 696, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.