RLG00000021354

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006964
Physical Location & Seq
Reverse (-)
74405570 .. 74409586
4017 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021354

Sequence Viewer

Length: 723 bp
ATGCTCCTCAGCTATGAGCTCCCAATCGGCATCACTCATATCGTCCGACTTGTAGGCCATGACGAAGAGCCAGAGAGACTTTCTGGTTGGGGATTTTCGATTGGAGATGAAGAGGCCGCGGGCCTTGTTGTCGTCGCCGGAGAATCAGTGCTCGTTCAGGGTCTGATACAGTCGCGCCTTGCCTTTAGGTCGTCGCCGGCGAGCAACGGCATCGAAGGTGAAGATGGTGGTGCAAGATCTGATAGCGCCGTGCTGGTACTCAGGGGAATCAAAGGCGGAGACAACGACGTGAAGGCGGTGGAACTCGAATTGGAGCAGCAGAGAAATAGAAGAGGAGCTTTCTCGTTGAAATTGGCGAGGAGCCTGACCTGCCGCCTCGTCCAAACGGCGTCACCGACGTCTTCACCGTCGGAGAAGAGCAAGGAGCTGCCGAGACCTGTCGACACCTGCCGAGCATCTGCCGAGCCCCTGCCGAGCAGCTGCCGAGACCTGCCGACACCTGCCGAGCATATGCCGAGCCCCTGCCGAGCAGCTACCGACACCTGCCGACACCTGCCGAGCATCTGCCGAGCCCCTGCCGAGACCTGCCGACACCTGCCTATACCTGCCGAGGCTCCTGCCGACAGCCCTGCGACATCTGCCGACAGCCCCTGCACGAGCCCTGCACAGCCTCCGCACAGCCTCCGCATAGCCCCTGCAGCGCCCCTGCACAGTCCAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

25.28

Weight (kDa)

5.82

Isoelectric Point (pI)

73.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 5 cut(s) 455, 508, 551, 561, 603
AatII GACGTC 1 cut(s) 401
Acc36I ACCTGC 9 cut(s) 377, 455, 498, 508, 551, 561, 593, 603, 613
AccI GTMKAC 1 cut(s) 441
AccII CGCG 2 cut(s) 119, 175
AciI CCGC 7 cut(s) 117, 119, 276, 296, 373, 674, 685
AcyI GRCGYC 2 cut(s) 389, 398
AfaI GTAC 1 cut(s) 258
AgsI TTSAA 1 cut(s) 349
AjiI CACGTC 1 cut(s) 289
AluBI AGCT 6 cut(s) 12, 19, 338, 427, 480, 533
AluI AGCT 6 cut(s) 12, 19, 338, 427, 480, 533
Alw21I GWGCWC 2 cut(s) 21, 153
Alw26I GTCTC 5 cut(s) 70, 273, 427, 480, 575
AlwNI CAGNNNCTG 2 cut(s) 163, 651
AoxI GGCC 3 cut(s) 55, 114, 121
ApeKI GCWGC 6 cut(s) 316, 427, 477, 480, 530, 698
AspLEI GCGC 3 cut(s) 177, 248, 703
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 3 cut(s) 230, 384, 396
BanII GRGCYC 5 cut(s) 21, 468, 521, 574, 662
BauI CACGAG 1 cut(s) 655
BbsI GAAGAC 1 cut(s) 393
Bbv12I GWGCWC 2 cut(s) 21, 153
BbvCI CCTCAGC 1 cut(s) 8
BbvI GCAGC 6 cut(s) 328, 414, 467, 489, 542, 710
BccI CCATC 1 cut(s) 218
BceAI ACGGC 3 cut(s) 223, 233, 402
BcgI CGANNNNNNTGC 6 cut(s) 193, 227, 599, 611, 633, 645
BcoDI GTCTC 5 cut(s) 70, 273, 427, 480, 575
BfaI CTAG 1 cut(s) 721
BfmI CTRYAG 1 cut(s) 696
BfoI RGCGCY 2 cut(s) 249, 704
BfuAI ACCTGC 9 cut(s) 377, 455, 498, 508, 551, 561, 593, 603, 613
BglII AGATCT 1 cut(s) 236
BisI GCNGC 8 cut(s) 117, 317, 373, 428, 478, 481, 531, 699
BlsI GCNGC 8 cut(s) 118, 318, 374, 429, 479, 482, 532, 700
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 2 cut(s) 362, 615
BmsI GCATC 4 cut(s) 39, 219, 464, 570
BpiI GAAGAC 1 cut(s) 393
Bpu10I CCTNAGC 1 cut(s) 8
BsaHI GRCGYC 2 cut(s) 389, 398
BsaI GGTCTC 3 cut(s) 427, 480, 575
BsaJI CCNNGG 2 cut(s) 117, 609
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bse118I RCCGGY 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 117, 609
BseMII CTCAG 2 cut(s) 22, 274
BseRI GAGGAG 2 cut(s) 348, 373
BseXI GCAGC 6 cut(s) 328, 414, 467, 489, 542, 710
BsgI GTGCAG 3 cut(s) 637, 648, 692
Bsh1236I CGCG 2 cut(s) 119, 175
BshFI GGCC 3 cut(s) 57, 116, 123
BsiHKAI GWGCWC 2 cut(s) 21, 153
BsiSI CCGG 2 cut(s) 138, 197
BsmAI GTCTC 5 cut(s) 70, 273, 427, 480, 575
BsnI GGCC 3 cut(s) 57, 116, 123
Bso31I GGTCTC 3 cut(s) 427, 480, 575
Bsp1286I GDGCHC 6 cut(s) 21, 153, 468, 521, 574, 662
Bsp143I GATC 1 cut(s) 236
BspACI CCGC 7 cut(s) 117, 119, 276, 296, 373, 674, 685
BspANI GGCC 3 cut(s) 57, 116, 123
BspCNI CTCAG 2 cut(s) 21, 273
BspFNI CGCG 2 cut(s) 119, 175
BspLI GGNNCC 2 cut(s) 362, 615
BspMAI CTGCAG 1 cut(s) 700
BspMI ACCTGC 9 cut(s) 377, 455, 498, 508, 551, 561, 593, 603, 613
BspQI GCTCTTC 2 cut(s) 60, 410
BspTNI GGTCTC 3 cut(s) 427, 480, 575
BsrFI RCCGGY 1 cut(s) 196
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 117, 609
BssMI GATC 1 cut(s) 236
BssNI GRCGYC 2 cut(s) 389, 398
BssSI CACGAG 1 cut(s) 655
Bst2BI CACGAG 1 cut(s) 655
Bst4CI ACNGT 3 cut(s) 171, 408, 713
Bst6I CTCTTC 4 cut(s) 60, 105, 325, 410
BstACI GRCGYC 2 cut(s) 389, 398
BstC8I GCNNGC 3 cut(s) 121, 198, 202
BstDEI CTNAG 2 cut(s) 8, 260
BstDSI CCRYGG 1 cut(s) 117
BstFNI CGCG 2 cut(s) 119, 175
BstH2I RGCGCY 2 cut(s) 249, 704
BstHHI GCGC 3 cut(s) 177, 248, 703
BstKTI GATC 1 cut(s) 239
BstMAI GTCTC 5 cut(s) 70, 273, 427, 480, 575
BstMBI GATC 1 cut(s) 236
BstMWI GCNNNNNNNGC 3 cut(s) 369, 638, 698
BstSFI CTRYAG 1 cut(s) 696
BstUI CGCG 2 cut(s) 119, 175
BstV1I GCAGC 6 cut(s) 328, 414, 467, 489, 542, 710
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BsuRI GGCC 3 cut(s) 57, 116, 123
BtgI CCRYGG 1 cut(s) 117
BtrI CACGTC 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 153
BveI ACCTGC 9 cut(s) 377, 455, 498, 508, 551, 561, 593, 603, 613
Cac8I GCNNGC 3 cut(s) 121, 198, 202
CaiI CAGNNNCTG 2 cut(s) 163, 651
CfoI GCGC 3 cut(s) 177, 248, 703
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 121
Cfr42I CCGCGG 1 cut(s) 120
CseI GACGC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 257
CviAII CATG 1 cut(s) 59
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 2 cut(s) 8, 260
DpnI GATC 1 cut(s) 238
DpnII GATC 1 cut(s) 236
Eam1104I CTCTTC 4 cut(s) 60, 105, 325, 410
EarI CTCTTC 4 cut(s) 60, 105, 325, 410
EciI GGCGGA 1 cut(s) 291
Ecl136II GAGCTC 1 cut(s) 19
Eco24I GRGCYC 5 cut(s) 21, 468, 521, 574, 662
Eco31I GGTCTC 3 cut(s) 427, 480, 575
Eco53kI GAGCTC 1 cut(s) 19
EcoICRI GAGCTC 1 cut(s) 19
EcoT38I GRGCYC 5 cut(s) 21, 468, 521, 574, 662
FaeI CATG 1 cut(s) 62
FaiI YATR 7 cut(s) 15, 39, 60, 510, 512, 602, 689
FalI AAGNNNNNCTT 2 cut(s) 322, 354
FatI CATG 1 cut(s) 58
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 1 cut(s) 510
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 8 cut(s) 117, 317, 373, 428, 478, 481, 531, 699
FriOI GRGCYC 5 cut(s) 21, 468, 521, 574, 662
Fsp4HI GCNGC 8 cut(s) 117, 317, 373, 428, 478, 481, 531, 699
FspBI CTAG 1 cut(s) 721
GlaI GCGC 3 cut(s) 176, 247, 702
GluI GCNGC 8 cut(s) 117, 317, 373, 428, 478, 481, 531, 699
HaeII RGCGCY 2 cut(s) 249, 704
HaeIII GGCC 3 cut(s) 57, 116, 123
HapII CCGG 2 cut(s) 138, 197
HgaI GACGC 1 cut(s) 378
HhaI GCGC 3 cut(s) 177, 248, 703
Hin1I GRCGYC 2 cut(s) 389, 398
Hin1II CATG 1 cut(s) 62
Hin6I GCGC 3 cut(s) 175, 246, 701
HinP1I GCGC 3 cut(s) 175, 246, 701
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 2 cut(s) 143, 267
HpaII CCGG 2 cut(s) 138, 197
HphI GGTGA 3 cut(s) 230, 384, 396
Hpy166II GTNNAC 1 cut(s) 442
Hpy188I TCNGA 4 cut(s) 47, 165, 241, 412
Hpy8I GTNNAC 1 cut(s) 442
Hpy99I CGWCG 5 cut(s) 137, 196, 290, 400, 412
HpyAV CCTTC 2 cut(s) 209, 286
HpyCH4III ACNGT 3 cut(s) 171, 408, 713
HpyCH4IV ACGT 2 cut(s) 288, 398
HpyCH4V TGCA 5 cut(s) 233, 654, 665, 698, 709
HpyF10VI GCNNNNNNNGC 3 cut(s) 369, 638, 698
HpyF3I CTNAG 2 cut(s) 8, 260
HpySE526I ACGT 2 cut(s) 288, 398
Hsp92I GRCGYC 2 cut(s) 389, 398
Hsp92II CATG 1 cut(s) 62
HspAI GCGC 3 cut(s) 175, 246, 701
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
KspI CCGCGG 1 cut(s) 120
Kzo9I GATC 1 cut(s) 236
LguI GCTCTTC 2 cut(s) 60, 410
LmnI GCTCC 7 cut(s) 9, 24, 313, 335, 360, 424, 619
Lsp1109I GCAGC 6 cut(s) 328, 414, 467, 489, 542, 710
LweI GCATC 4 cut(s) 39, 219, 464, 570
MaeI CTAG 1 cut(s) 721
MaeII ACGT 2 cut(s) 288, 398
MaeIII GTNAC 1 cut(s) 390
MalI GATC 1 cut(s) 238
MboI GATC 1 cut(s) 236
MboII GAAGA 6 cut(s) 77, 122, 233, 342, 393, 427
MflI RGATCY 1 cut(s) 236
MhlI GDGCHC 6 cut(s) 21, 153, 468, 521, 574, 662
MluCI AATT 2 cut(s) 308, 350
MmeI TCCRAC 2 cut(s) 70, 390
MnlI CCTC 8 cut(s) 17, 106, 326, 351, 386, 604, 681, 692
MreI CGCCGGCG 1 cut(s) 196
MroNI GCCGGC 1 cut(s) 196
MspA1I CMGCKG 2 cut(s) 119, 480
MspI CCGG 2 cut(s) 138, 197
MvnI CGCG 2 cut(s) 119, 175
MwoI GCNNNNNNNGC 3 cut(s) 369, 638, 698
NaeI GCCGGC 1 cut(s) 198
NdeI CATATG 1 cut(s) 510
NdeII GATC 1 cut(s) 236
NgoMIV GCCGGC 1 cut(s) 196
NlaIII CATG 1 cut(s) 62
NlaIV GGNNCC 2 cut(s) 362, 615
NmuCI GTSAC 1 cut(s) 390
PaqCI CACCTGC 5 cut(s) 455, 508, 551, 561, 603
PciSI GCTCTTC 2 cut(s) 60, 410
PcsI WCGNNNNNNNCGW 3 cut(s) 197, 392, 404
PdiI GCCGGC 1 cut(s) 198
PfeI GAWTC 2 cut(s) 143, 267
PkrI GCNGC 8 cut(s) 118, 318, 374, 429, 479, 482, 532, 700
Psp124BI GAGCTC 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 362, 615
PspPI GGNCC 1 cut(s) 121
PstI CTGCAG 1 cut(s) 700
PstNI CAGNNNCTG 2 cut(s) 163, 651
PsuI RGATCY 1 cut(s) 236
PvuII CAGCTG 1 cut(s) 480
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
SacI GAGCTC 1 cut(s) 21
SacII CCGCGG 1 cut(s) 120
SalI GTCGAC 1 cut(s) 440
SapI GCTCTTC 2 cut(s) 60, 410
SatI GCNGC 8 cut(s) 117, 317, 373, 428, 478, 481, 531, 699
Sau3AI GATC 1 cut(s) 236
Sau96I GGNCC 1 cut(s) 121
SduI GDGCHC 6 cut(s) 21, 153, 468, 521, 574, 662
SfaNI GCATC 4 cut(s) 39, 219, 464, 570
SfcI CTRYAG 1 cut(s) 696
Sfr303I CCGCGG 1 cut(s) 120
SgrAI CRCCGGYG 1 cut(s) 196
SgrBI CCGCGG 1 cut(s) 120
Sse9I AATT 2 cut(s) 308, 350
SsiI CCGC 7 cut(s) 117, 119, 276, 296, 373, 674, 685
SspMI CTAG 1 cut(s) 721
SstI GAGCTC 1 cut(s) 21
TaaI ACNGT 3 cut(s) 171, 408, 713
TaiI ACGT 2 cut(s) 291, 401
TaqI TCGA 4 cut(s) 98, 213, 306, 441
TasI AATT 2 cut(s) 308, 350
TauI GCSGC 2 cut(s) 119, 375
TfiI GAWTC 2 cut(s) 143, 267
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 1 cut(s) 390
TseI GCWGC 6 cut(s) 316, 427, 477, 480, 530, 698
Tsp45I GTSAC 1 cut(s) 390
TspDTI ATGAA 1 cut(s) 123
TspRI CASTG 1 cut(s) 153
XmiI GTMKAC 1 cut(s) 441
XspI CTAG 1 cut(s) 721
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.