Rorug02G0340800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006964
Physical Location & Seq
Forward (+)
42410284 .. 42410826
543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0340800.1

Sequence Viewer

Length: 444 bp
ATGCCGGTGCAAGTACCCATTTCTTCTGTTCGTAGAGTGCACGGTAACTCTTCTTATGCTGGTTCTCACACTGAATCTAAAGAAAAGCATGCCGGGAGTGGTGTATATAAACAAAGATTAATTAGTGGTGATCAAGAAGCTGGGCTGCATGAAGATAACATTGATTTGAAACGTGAATCCAACCAGATCAGTAACAGAAGTGATGGTCTGACATTAGATGGGTCATCAGTAAACAGGCGTCTGCAGGGTAGTGGCATATCACCCTACCGTAGGGAATGTTCTGAGTCTATCCTTCATGATGGAAAATGTTCTCTTGGTATTCCTGAGAAAGCTAAGAGTTCCAAGGAGACTAGTAGCTCGGATAAATATAGAAAAGGCCGCAGCTTTCTGGAATGGGAAGCGGGCTTAGGATGTCCTGTGGTTGAGAAGACACTGTTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

16.06

Weight (kDa)

8.69

Isoelectric Point (pI)

61.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 7 - 125 8.1e-08 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 379, 401
AcyI GRCGYC 1 cut(s) 238
AfaI GTAC 1 cut(s) 15
AfiI CCNNNNNNNGG 1 cut(s) 270
AgsI TTSAA 1 cut(s) 169
AhlI ACTAGT 1 cut(s) 350
AluBI AGCT 4 cut(s) 140, 332, 357, 384
AluI AGCT 4 cut(s) 140, 332, 357, 384
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 341
Alw44I GTGCAC 1 cut(s) 38
AoxI GGCC 1 cut(s) 376
ApaLI GTGCAC 1 cut(s) 38
ApeKI GCWGC 2 cut(s) 145, 381
AseI ATTAAT 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 2 cut(s) 140, 252
BaeGI GKGCMC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 132, 393
BccI CCATC 3 cut(s) 197, 212, 293
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 341
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 242
BisI GCNGC 3 cut(s) 146, 379, 382
BlsI GCNGC 3 cut(s) 147, 380, 383
Bme1390I CCNGG 1 cut(s) 94
BmrFI CCNGG 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 434
Bpu10I CCTNAGC 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 94
BsaHI GRCGYC 1 cut(s) 238
BsaJI CCNNGG 1 cut(s) 342
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 4
BseDI CCNNGG 1 cut(s) 342
BseGI GGATG 1 cut(s) 416
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMII CTCAG 2 cut(s) 273, 315
BseSI GKGCMC 1 cut(s) 42
BseXI GCAGC 2 cut(s) 132, 393
BseYI CCCAGC 1 cut(s) 140
BshFI GGCC 1 cut(s) 378
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 2 cut(s) 5, 93
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 341
BsnI GGCC 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 2 cut(s) 130, 186
BspACI CCGC 2 cut(s) 379, 401
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 2 cut(s) 274, 316
BspHI TCATGA 1 cut(s) 295
BspMAI CTGCAG 1 cut(s) 246
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 342
BssMI GATC 2 cut(s) 130, 186
BssNI GRCGYC 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 342
Bst4CI ACNGT 3 cut(s) 44, 269, 435
Bst6I CTCTTC 1 cut(s) 55
BstACI GRCGYC 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 90, 403
BstDEI CTNAG 4 cut(s) 282, 324, 333, 406
BstENI CCTNNNNNAGG 1 cut(s) 268
BstF5I GGATG 1 cut(s) 416
BstKTI GATC 2 cut(s) 133, 189
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 2 cut(s) 130, 186
BstNSI RCATGY 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 92
BstSFI CTRYAG 1 cut(s) 242
BstSLI GKGCMC 1 cut(s) 42
BstV1I GCAGC 2 cut(s) 132, 393
BstV2I GAAGAC 1 cut(s) 434
BsuRI GGCC 1 cut(s) 378
BtsCI GGATG 1 cut(s) 416
BtsIMutI CAGTG 2 cut(s) 69, 431
Cac8I GCNNGC 2 cut(s) 90, 403
CciI TCATGA 1 cut(s) 295
Cfr10I RCCGGY 1 cut(s) 4
CseI GACGC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 14
CviAII CATG 3 cut(s) 89, 149, 296
CviJI RGCY 7 cut(s) 140, 145, 332, 357, 378, 384, 405
CviKI_1 RGCY 7 cut(s) 140, 145, 332, 357, 378, 384, 405
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 4 cut(s) 282, 324, 333, 406
DpnI GATC 2 cut(s) 132, 188
DpnII GATC 2 cut(s) 130, 186
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 342
EcoNI CCTNNNNNAGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 3 cut(s) 92, 152, 299
FaiI YATR 9 cut(s) 57, 90, 106, 108, 150, 257, 297, 369, 439
FatI CATG 3 cut(s) 88, 148, 295
FauI CCCGC 1 cut(s) 394
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 3 cut(s) 146, 379, 382
FokI GGATG 1 cut(s) 423
Fsp4HI GCNGC 3 cut(s) 146, 379, 382
FspBI CTAG 1 cut(s) 351
GluI GCNGC 3 cut(s) 146, 379, 382
GsaI CCCAGC 1 cut(s) 144
HaeIII GGCC 1 cut(s) 378
HapII CCGG 2 cut(s) 5, 93
HgaI GACGC 1 cut(s) 227
Hin1I GRCGYC 1 cut(s) 238
Hin1II CATG 3 cut(s) 92, 152, 299
HinfI GANTC 3 cut(s) 74, 176, 284
HpaII CCGG 2 cut(s) 5, 93
HphI GGTGA 2 cut(s) 140, 252
Hpy166II GTNNAC 2 cut(s) 40, 232
Hpy188I TCNGA 3 cut(s) 210, 283, 361
Hpy188III TCNNGA 4 cut(s) 134, 296, 323, 389
Hpy8I GTNNAC 2 cut(s) 40, 232
HpyAV CCTTC 1 cut(s) 302
HpyCH4III ACNGT 3 cut(s) 44, 269, 435
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 4 cut(s) 10, 40, 148, 244
HpyF3I CTNAG 4 cut(s) 282, 324, 333, 406
HpySE526I ACGT 1 cut(s) 172
Hsp92I GRCGYC 1 cut(s) 238
Hsp92II CATG 3 cut(s) 92, 152, 299
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 2 cut(s) 130, 186
Lsp1109I GCAGC 2 cut(s) 132, 393
MaeI CTAG 1 cut(s) 351
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 2 cut(s) 44, 191
MalI GATC 2 cut(s) 132, 188
MboI GATC 2 cut(s) 130, 186
MboII GAAGA 4 cut(s) 15, 42, 164, 439
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 1 cut(s) 120
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 204
MroXI GAANNNNTTC 1 cut(s) 307
MseI TTAA 1 cut(s) 119
MslI CAYNNNNRTG 1 cut(s) 436
MspI CCGG 2 cut(s) 5, 93
MspR9I CCNGG 1 cut(s) 94
NciI CCSGG 1 cut(s) 94
NdeII GATC 2 cut(s) 130, 186
NlaIII CATG 3 cut(s) 92, 152, 299
NspI RCATGY 1 cut(s) 92
PaeI GCATGC 1 cut(s) 92
PagI TCATGA 1 cut(s) 295
PdmI GAANNNNTTC 1 cut(s) 307
PfeI GAWTC 2 cut(s) 74, 176
PkrI GCNGC 3 cut(s) 147, 380, 383
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
PshBI ATTAAT 1 cut(s) 119
PspFI CCCAGC 1 cut(s) 140
PsrI GAACNNNNNNTAC 2 cut(s) 262, 294
PstI CTGCAG 1 cut(s) 246
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
RseI CAYNNNNRTG 1 cut(s) 436
SaqAI TTAA 1 cut(s) 119
SatI GCNGC 3 cut(s) 146, 379, 382
Sau3AI GATC 2 cut(s) 130, 186
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 1 cut(s) 42
SetI ASST 5 cut(s) 142, 175, 334, 359, 386
SfcI CTRYAG 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 436
SpeI ACTAGT 1 cut(s) 350
SphI GCATGC 1 cut(s) 92
Sse9I AATT 1 cut(s) 120
SsiI CCGC 2 cut(s) 379, 401
SspMI CTAG 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 3 cut(s) 44, 269, 435
TaiI ACGT 1 cut(s) 175
TasI AATT 1 cut(s) 120
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 2 cut(s) 74, 176
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TscAI CASTG 2 cut(s) 76, 438
TseI GCWGC 2 cut(s) 145, 381
TspDTI ATGAA 2 cut(s) 165, 284
TspRI CASTG 2 cut(s) 76, 438
VneI GTGCAC 1 cut(s) 38
VspI ATTAAT 1 cut(s) 119
XagI CCTNNNNNAGG 1 cut(s) 268
XceI RCATGY 1 cut(s) 92
XmnI GAANNNNTTC 1 cut(s) 307
XspI CTAG 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.