RLG00000023404

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001107
Physical Location & Seq
Reverse (-)
23155596 .. 23162299
6704 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023404

Sequence Viewer

Length: 1536 bp
ATGGCTTTATATACTCTCCTAAATTGTTGCAAGAATATGGGCAAGATGCCCCTCCTTGTGTTCTTCAGACACACAAGGAATGTTTTCAAAGCTGATGAGCTAGGTGTGGAGATAGTACATATTGCACTTCCTACGCTACTAGCGTTAGTAGCAGACCCTGCTGCTTCTCTAATTGACACAGCATTTGTTGGCCATATAGGTCCAGTGGAGCTCGCAGCTGTGGGAATTTCTATTGCTGTTTTCAATCAAGTATCAAAAATAGCAATTTTCCCACTTGTCAGTATTACAACCTCTTTTGTTGCTGAAGAAGATACTACGGAACCATTAAGTTCTAAAGAACAAGCATATGAAGAATTTGAAAATGCTACTTCCGTCAACAAGGAAATGGAAGTACTCAAACCACTTGTTGACTCTACAGCATCATCCATTACCATTGGTAGTACAGAATTGGTCAAACTTGAGCATGTCAGAAGGCGTATTCCATCTGCTTCATCAGCATTGGTTATTGGCAGCGTCCTGGGGGTTATTCAAACTCTATTCCTAATTTTTGCTGCAAACCCCATCCTAAATTACATGGGGGTTGATTCTAATTCTCCTATGTTAAGACCAGCGAGACAATACCTGACACTAAGGTCACTTGGTGCTCCTGCAGTTCTTCTCTCTTTGGCCATGCAAGGGGTTTTCCGTGGTTTTAAGGATACAAAAACTCCTTTATATGCTACAATTGTGGGAGATGCAGTAAACATCATTTTGGACCCAATATTAATGTTTGTACTCCATATGGGCATTACCGGTGCGGCCATTGCCCATGTTATCTCTCAGTATTTAATCTCCCTGATACTGTTATGGAGATTAAACAAACAAGTTGATCTCCTAACCCCAGGCATCAAAGATCTACGAATTGGCCAGTTTCTGAAAAATGGGGGTATGTTATTGGTGAGAGTTATAGCTGCAACATTCTGTGTCACCCTGGCAGCATCATTAGCTGCAAGGCAGGGATCGACATCAATGGCAGCATTTCAAGTTTGCTTGCAGATTTGGCTTGCAGCCTCTTTGCTTGCGGATGGGTTAGCTGTGGCTGGACAAGCAATCCTTGCAAGTGCATTTGCGAGGAAGGATTACAGCAAGGTCATTGCCACAGCTTCACGTGTATTGCAGATGGCTTTGGTTCTAGGGATAGTGCTCTCAGTCATCCTTACAGTTGTACTAAAGTTCGCGTCAAGATTATTTACCGATGATATCAATGTTTTGCACCTTATAAGTTTAGGCATTCCGTTTATTGCAGTGACTCAGCCCGTTAATGCTCTGGCATTTGTTTTTGATGGAGTAAATTATGGAGCATCAGATTTTGCATATTCTGCCTACTCAATGGTGTTGGTGGCTTTAGTGAGCATATTGTGCCTGTTTCTATTATCATCAAGCCATGGCTTTGTTGGTATCTGGGTTGCTTTAACAATCTTCATGAGTTTGCGCACAGTGGTTGGATTTTGGAGGATAGGGACTGGAACTGGACCATGGAGCTTTCTGAGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

512

Amino Acids

54.92

Weight (kDa)

8.51

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 163 - 254 1.7e-12 MatE
MatE PF01554 314 - 466 1.1e-12 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1259
AasI GACNNNNNNGTC 1 cut(s) 631
Acc16I TGCGCA 1 cut(s) 1472
AccII CGCG 1 cut(s) 1217
AciI CCGC 2 cut(s) 797, 1061
AclWI GGATC 1 cut(s) 1006
AcoI YGGCCR 4 cut(s) 190, 666, 798, 904
AcsI RAATTY 2 cut(s) 225, 353
AcuI CTGAAG 2 cut(s) 49, 324
AcvI CACGTG 1 cut(s) 1148
AdeI CACNNNGTG 1 cut(s) 641
AfaI GTAC 5 cut(s) 117, 393, 442, 774, 1206
AfiI CCNNNNNNNGG 1 cut(s) 675
AflIII ACRYGT 1 cut(s) 1147
AgeI ACCGGT 1 cut(s) 791
AgsI TTSAA 5 cut(s) 88, 244, 359, 530, 1022
AjnI CCWGG 3 cut(s) 516, 880, 969
AluBI AGCT 9 cut(s) 92, 100, 211, 218, 950, 986, 1073, 1142, 1521
AluI AGCT 9 cut(s) 92, 100, 211, 218, 950, 986, 1073, 1142, 1521
Alw21I GWGCWC 3 cut(s) 213, 646, 1185
Alw26I GTCTC 1 cut(s) 607
AlwI GGATC 1 cut(s) 1006
AlwNI CAGNNNCTG 2 cut(s) 158, 913
AoxI GGCC 4 cut(s) 190, 666, 798, 904
ApeKI GCWGC 9 cut(s) 161, 215, 510, 551, 950, 974, 986, 1013, 1046
ApoI RAATTY 2 cut(s) 225, 353
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AseI ATTAAT 1 cut(s) 764
AsiGI ACCGGT 1 cut(s) 791
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 1473
AspS9I GGNCC 3 cut(s) 200, 754, 1511
AsuHPI GGTGA 2 cut(s) 949, 958
AvaII GGWCC 3 cut(s) 200, 754, 1511
BalI TGGCCA 3 cut(s) 192, 668, 906
BanII GRGCYC 1 cut(s) 213
BbrPI CACGTG 1 cut(s) 1148
Bbv12I GWGCWC 3 cut(s) 213, 646, 1185
BbvI GCAGC 9 cut(s) 148, 227, 522, 538, 937, 973, 986, 1025, 1058
BccI CCATC 5 cut(s) 490, 569, 1058, 1153, 1316
BciT130I CCWGG 3 cut(s) 518, 882, 971
BciVI GTATCC 1 cut(s) 691
BcoDI GTCTC 1 cut(s) 607
BfaI CTAG 3 cut(s) 101, 140, 1172
BfmI CTRYAG 2 cut(s) 414, 648
BfuI GTATCC 1 cut(s) 691
BglII AGATCT 1 cut(s) 892
BmcAI AGTACT 1 cut(s) 393
Bme1390I CCNGG 3 cut(s) 518, 882, 971
Bme18I GGWCC 3 cut(s) 200, 754, 1511
BmgT120I GGNCC 3 cut(s) 200, 754, 1511
BmiI GGNNCC 2 cut(s) 321, 756
BmrFI CCNGG 3 cut(s) 518, 882, 971
BmsI GCATC 6 cut(s) 36, 428, 724, 894, 986, 1349
BpuEI CTTGAG 1 cut(s) 479
BsaAI YACGTR 1 cut(s) 1148
BsaBI GATNNNNATC 1 cut(s) 1003
BsaJI CCNNGG 6 cut(s) 517, 685, 880, 969, 1423, 1514
BsaWI WCCGGW 1 cut(s) 791
BsaXI ACNNNNNCTCC 4 cut(s) 691, 721, 723, 753
Bsc4I CCNNNNNNNGG 1 cut(s) 675
Bse118I RCCGGY 1 cut(s) 791
Bse1I ACTGG 4 cut(s) 203, 907, 1507, 1513
Bse3DI GCAATG 2 cut(s) 801, 1131
Bse8I GATNNNNATC 1 cut(s) 1003
BseBI CCWGG 3 cut(s) 518, 882, 971
BseDI CCNNGG 6 cut(s) 517, 685, 880, 969, 1423, 1514
BseGI GGATG 4 cut(s) 422, 561, 1069, 1191
BseJI GATNNNNATC 1 cut(s) 1003
BseLI CCNNNNNNNGG 1 cut(s) 675
BseMI GCAATG 2 cut(s) 801, 1131
BseMII CTCAG 4 cut(s) 833, 1200, 1304, 1517
BseNI ACTGG 4 cut(s) 203, 907, 1507, 1513
BseXI GCAGC 9 cut(s) 148, 227, 522, 538, 937, 973, 986, 1025, 1058
Bsh1236I CGCG 1 cut(s) 1217
BshFI GGCC 4 cut(s) 192, 668, 800, 906
BshTI ACCGGT 1 cut(s) 791
BsiHKAI GWGCWC 3 cut(s) 213, 646, 1185
BsiSI CCGG 1 cut(s) 792
BslFI GGGAC 1 cut(s) 1513
BslI CCNNNNNNNGG 1 cut(s) 675
BsmAI GTCTC 1 cut(s) 607
BsmFI GGGAC 1 cut(s) 1513
BsmI GAATGC 1 cut(s) 1269
BsnI GGCC 4 cut(s) 192, 668, 800, 906
Bsp1286I GDGCHC 3 cut(s) 213, 646, 1185
Bsp143I GATC 3 cut(s) 868, 892, 998
Bsp19I CCATGG 2 cut(s) 1423, 1514
BspACI CCGC 2 cut(s) 797, 1061
BspANI GGCC 4 cut(s) 192, 668, 800, 906
BspCNI CTCAG 4 cut(s) 832, 1199, 1303, 1518
BspFNI CGCG 1 cut(s) 1217
BspHI TCATGA 1 cut(s) 1461
BspLI GGNNCC 2 cut(s) 321, 756
BspMAI CTGCAG 1 cut(s) 652
BspPI GGATC 1 cut(s) 1006
BsrDI GCAATG 2 cut(s) 801, 1131
BsrFI RCCGGY 1 cut(s) 791
BsrI ACTGG 4 cut(s) 203, 907, 1507, 1513
BssAI RCCGGY 1 cut(s) 791
BssECI CCNNGG 6 cut(s) 517, 685, 880, 969, 1423, 1514
BssMI GATC 3 cut(s) 868, 892, 998
BssT1I CCWWGG 2 cut(s) 1423, 1514
Bst2UI CCWGG 3 cut(s) 518, 882, 971
Bst4CI ACNGT 3 cut(s) 843, 1201, 1477
BstAPI GCANNNNNTGC 4 cut(s) 158, 1094, 1358, 1398
BstBAI YACGTR 1 cut(s) 1148
BstC8I GCNNGC 4 cut(s) 213, 1031, 1044, 1059
BstDEI CTNAG 5 cut(s) 629, 819, 1186, 1290, 1526
BstDSI CCRYGG 3 cut(s) 685, 1423, 1514
BstF5I GGATG 4 cut(s) 422, 561, 1069, 1191
BstFNI CGCG 1 cut(s) 1217
BstHHI GCGC 1 cut(s) 1473
BstKTI GATC 3 cut(s) 871, 895, 1001
BstMAI GTCTC 1 cut(s) 607
BstMBI GATC 3 cut(s) 868, 892, 998
BstNI CCWGG 3 cut(s) 518, 882, 971
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 3 cut(s) 516, 880, 969
BstSFI CTRYAG 2 cut(s) 414, 648
BstUI CGCG 1 cut(s) 1217
BstV1I GCAGC 9 cut(s) 148, 227, 522, 538, 937, 973, 986, 1025, 1058
BstX2I RGATCY 1 cut(s) 892
BstYI RGATCY 1 cut(s) 892
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 4 cut(s) 192, 668, 800, 906
BtgI CCRYGG 3 cut(s) 685, 1423, 1514
BtsCI GGATG 4 cut(s) 422, 561, 1069, 1191
BtsI GCAGTG 1 cut(s) 1290
BtsIMutI CAGTG 3 cut(s) 210, 1290, 1482
Cac8I GCNNGC 4 cut(s) 213, 1031, 1044, 1059
CaiI CAGNNNCTG 2 cut(s) 158, 913
CciI TCATGA 1 cut(s) 1461
CfoI GCGC 1 cut(s) 1473
Cfr10I RCCGGY 1 cut(s) 791
Cfr13I GGNCC 3 cut(s) 200, 754, 1511
CseI GACGC 2 cut(s) 502, 1206
Csp6I GTAC 5 cut(s) 116, 392, 441, 773, 1205
CspAI ACCGGT 1 cut(s) 791
CviAII CATG 7 cut(s) 464, 574, 670, 809, 1424, 1462, 1515
CviQI GTAC 5 cut(s) 116, 392, 441, 773, 1205
DdeI CTNAG 5 cut(s) 629, 819, 1186, 1290, 1526
DpnI GATC 3 cut(s) 870, 894, 1000
DpnII GATC 3 cut(s) 868, 892, 998
DraIII CACNNNGTG 1 cut(s) 641
DrdI GACNNNNNNGTC 1 cut(s) 631
DseDI GACNNNNNNGTC 1 cut(s) 631
EaeI YGGCCR 4 cut(s) 190, 666, 798, 904
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 1423, 1514
Eco24I GRGCYC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 1240
Eco47I GGWCC 3 cut(s) 200, 754, 1511
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 49, 324
Eco72I CACGTG 1 cut(s) 1148
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 3 cut(s) 516, 880, 969
EcoRV GATATC 1 cut(s) 1240
EcoT14I CCWWGG 2 cut(s) 1423, 1514
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 2 cut(s) 1423, 1514
FaeI CATG 7 cut(s) 467, 577, 673, 812, 1427, 1465, 1518
FalI AAGNNNNNCTT 2 cut(s) 1077, 1109
FaqI GGGAC 1 cut(s) 1513
FatI CATG 7 cut(s) 463, 573, 669, 808, 1423, 1461, 1514
FauNDI CATATG 2 cut(s) 346, 780
FokI GGATG 4 cut(s) 409, 548, 1076, 1178
FriOI GRGCYC 1 cut(s) 213
FspBI CTAG 3 cut(s) 101, 140, 1172
FspI TGCGCA 1 cut(s) 1472
GlaI GCGC 1 cut(s) 1472
HaeIII GGCC 4 cut(s) 192, 668, 800, 906
HapII CCGG 1 cut(s) 792
HgaI GACGC 2 cut(s) 502, 1206
HhaI GCGC 1 cut(s) 1473
Hin1II CATG 7 cut(s) 467, 577, 673, 812, 1427, 1465, 1518
Hin6I GCGC 1 cut(s) 1471
HinP1I GCGC 1 cut(s) 1471
HincII GTYRAC 2 cut(s) 376, 409
HindII GTYRAC 2 cut(s) 376, 409
HinfI GANTC 3 cut(s) 410, 584, 1288
HpaII CCGG 1 cut(s) 792
HphI GGTGA 2 cut(s) 949, 958
Hpy166II GTNNAC 3 cut(s) 376, 409, 742
Hpy188I TCNGA 5 cut(s) 68, 470, 915, 1345, 1527
Hpy188III TCNNGA 2 cut(s) 1221, 1462
Hpy8I GTNNAC 3 cut(s) 376, 409, 742
HpyAV CCTTC 2 cut(s) 465, 1108
HpyCH4III ACNGT 3 cut(s) 843, 1201, 1477
HpyCH4IV ACGT 1 cut(s) 1147
HpyF3I CTNAG 5 cut(s) 629, 819, 1186, 1290, 1526
HpySE526I ACGT 1 cut(s) 1147
Hsp92II CATG 7 cut(s) 467, 577, 673, 812, 1427, 1465, 1518
HspAI GCGC 1 cut(s) 1471
Kzo9I GATC 3 cut(s) 868, 892, 998
LmnI GCTCC 4 cut(s) 208, 649, 1337, 1518
Lsp1109I GCAGC 9 cut(s) 148, 227, 522, 538, 937, 973, 986, 1025, 1058
LweI GCATC 6 cut(s) 36, 428, 724, 894, 986, 1349
MaeI CTAG 3 cut(s) 101, 140, 1172
MaeII ACGT 1 cut(s) 1147
MaeIII GTNAC 3 cut(s) 633, 964, 1285
MalI GATC 3 cut(s) 870, 894, 1000
MboI GATC 3 cut(s) 868, 892, 998
MboII GAAGA 6 cut(s) 55, 317, 320, 362, 647, 1450
MfeI CAATTG 1 cut(s) 723
MflI RGATCY 1 cut(s) 892
MhlI GDGCHC 3 cut(s) 213, 646, 1185
MlsI TGGCCA 3 cut(s) 192, 668, 906
MluNI TGGCCA 3 cut(s) 192, 668, 906
MlyI GAGTC 2 cut(s) 404, 1282
MmeI TCCRAC 1 cut(s) 1462
MnlI CCTC 6 cut(s) 62, 301, 1060, 1104, 1485, 1521
Mox20I TGGCCA 3 cut(s) 192, 668, 906
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 3 cut(s) 192, 668, 906
MseI TTAA 8 cut(s) 326, 602, 693, 764, 827, 854, 1299, 1451
Msp20I TGGCCA 3 cut(s) 192, 668, 906
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 1 cut(s) 792
MspR9I CCNGG 3 cut(s) 518, 882, 971
MunI CAATTG 1 cut(s) 723
Mva1269I GAATGC 1 cut(s) 1269
MvaI CCWGG 3 cut(s) 518, 882, 971
MvnI CGCG 1 cut(s) 1217
NcoI CCATGG 2 cut(s) 1423, 1514
NdeI CATATG 2 cut(s) 346, 780
NdeII GATC 3 cut(s) 868, 892, 998
NlaIII CATG 7 cut(s) 467, 577, 673, 812, 1427, 1465, 1518
NlaIV GGNNCC 2 cut(s) 321, 756
NmuCI GTSAC 3 cut(s) 633, 964, 1285
NsbI TGCGCA 1 cut(s) 1472
NspI RCATGY 1 cut(s) 467
PagI TCATGA 1 cut(s) 1461
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PctI GAATGC 1 cut(s) 1269
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 584
PinAI ACCGGT 1 cut(s) 791
PleI GAGTC 2 cut(s) 404, 1282
PmaCI CACGTG 1 cut(s) 1148
PmlI CACGTG 1 cut(s) 1148
PpsI GAGTC 2 cut(s) 404, 1282
Ppu21I YACGTR 1 cut(s) 1148
PshBI ATTAAT 1 cut(s) 764
PsiI TTATAA 1 cut(s) 1259
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 3 cut(s) 516, 880, 969
PspCI CACGTG 1 cut(s) 1148
PspGI CCWGG 3 cut(s) 516, 880, 969
PspN4I GGNNCC 2 cut(s) 321, 756
PspPI GGNCC 3 cut(s) 200, 754, 1511
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 2 cut(s) 158, 913
PsuI RGATCY 1 cut(s) 892
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 5 cut(s) 117, 393, 442, 774, 1206
RsaNI GTAC 5 cut(s) 116, 392, 441, 773, 1205
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 8 cut(s) 326, 602, 693, 764, 827, 854, 1299, 1451
Sau3AI GATC 3 cut(s) 868, 892, 998
Sau96I GGNCC 3 cut(s) 200, 754, 1511
ScaI AGTACT 1 cut(s) 393
SchI GAGTC 2 cut(s) 404, 1282
ScrFI CCNGG 3 cut(s) 518, 882, 971
SduI GDGCHC 3 cut(s) 213, 646, 1185
SfaNI GCATC 6 cut(s) 36, 428, 724, 894, 986, 1349
SfcI CTRYAG 2 cut(s) 414, 648
SinI GGWCC 3 cut(s) 200, 754, 1511
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SsiI CCGC 2 cut(s) 797, 1061
SspI AATATT 1 cut(s) 762
SspMI CTAG 3 cut(s) 101, 140, 1172
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 3 cut(s) 516, 880, 969
StyI CCWWGG 2 cut(s) 1423, 1514
TaaI ACNGT 3 cut(s) 843, 1201, 1477
TaiI ACGT 1 cut(s) 1150
TaqI TCGA 1 cut(s) 1001
TatI WGTACW 5 cut(s) 115, 391, 440, 772, 1204
TauI GCSGC 1 cut(s) 800
TfiI GAWTC 1 cut(s) 584
Tru1I TTAA 8 cut(s) 326, 602, 693, 764, 827, 854, 1299, 1451
Tru9I TTAA 8 cut(s) 326, 602, 693, 764, 827, 854, 1299, 1451
TscAI CASTG 3 cut(s) 210, 1290, 1482
TseFI GTSAC 3 cut(s) 633, 964, 1285
TseI GCWGC 9 cut(s) 161, 215, 510, 551, 950, 974, 986, 1013, 1046
Tsp45I GTSAC 3 cut(s) 633, 964, 1285
TspDTI ATGAA 3 cut(s) 363, 480, 1450
TspGWI ACGGA 4 cut(s) 332, 361, 674, 1263
TspRI CASTG 3 cut(s) 210, 1290, 1482
VpaK11BI GGWCC 3 cut(s) 200, 754, 1511
VspI ATTAAT 1 cut(s) 764
XapI RAATTY 2 cut(s) 225, 353
XceI RCATGY 1 cut(s) 467
XcmI CCANNNNNNNNNTGG 1 cut(s) 1430
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 3 cut(s) 101, 140, 1172
ZrmI AGTACT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.