Rh3DG269200

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001107
Physical Location & Seq
Forward (+)
26938207 .. 26944979
6773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG269200.1

Sequence Viewer

Length: 1500 bp
ATGGGCAAGATGCCCCTCCTTGTGTTCTTCAAAGACACAAGGAATGTTTTCAAAGCTGATGAGCTAGGCGTGGAGATAGTACATATTGCACTTCCTACGCTACTAGCGTTAGTAGCAGACCCTGCTGCTTCTCTAATTGACACAGCATTTGTTGGCCATATAGGTCCAGTGGAGCTCGCAGCTGTGGGAATTTCTATTGCTGTTTTCAATCAAGTATCAAAAATAGCAATTTTCCCACTTGTCAGTATTACAACCTCTTTTGTTGCTGAAGAAGATACTACGGAACCATTAAGTACTAAAGAACAAGTATATGAAGAATTTGAAAATGCTACTTCTGTCAACAAGGAAATGGACGTACTCAAACCACTTGTTGACTCTACACCATCATCCATTTCCAATGATAGTACAGAAATGGTCAAACTTGAGCATGTCAGAAGGCGTATTCCATCTGCTTCATCAGCATTGGTTATTGGCAGCGTCCTGGGGGTTGTTCAAACTCTATTCCTTATTTTTGCTGCAAACCCCATCCTAAATTACATGGGGGTAGATTCTAATTCTCCTATGTTAAGACCAGCGAGACAATACCTGACACTAAGGTCACTTGGTGCTCCTGCAGTTCTTCTCTCTTTGGCCATGCAGGGGGTTTTCCGTGGTTTTAAGGATACAAAAACTCCTTTATATGCTACAATTGTGGGAGATACAGTAAACATCATTTTGGACCCAATATTAATGTTTGTACTCCATATGGGCATTACCGGTGCGGCCATTGCCCATGTTATCTCTCAGTATTTAATCTCCCTGATACTGTTATGGAGATTAAACAAACAAGTTGATCTCCTAACCCCAGGCATCAAAGATCTACGAATTGGCCAGTTTCTGAAAAATGGGGGTATGTTATTGGTGAGAGTTATAGCTGCAACATTCTGTGTCACCCTGGCAGCATCATTAGCTGCAAGGCAGGGATCGACATCAATGGCAGCATTTCAAGTTTGCTTGCAGATTTGGCTTGCAGCCTCTTTGCTTGCGGATGGGTTAGCTGTGGCTGGACAAGCAATCCTTGCAAGTGCATTTGCAAGGAAGGATCACAGCAAGGTCGTTGCCACAGCTTCACGTGTATTGCAGATGGCTTTGGTTCTAGGGATAGTGCTCTCAGTCATCCTTACAGTTGTACTAAAGTTTGCGTCAAGATTATTTACCGATGATATCGATGTTTTGCACCTTATAAGTTTAGGCATTCCGTTTATTGCAGTGACTCAGCCCATTAATGCTCTGGCATTTGTTTTTGATGGAGTAAATTATGGAGCATCAGATTTTGCATATTCTGCCTACTCAATGGTGTTGGTGGCTTTAGTGAGCATATTGTGCCTGTTTCTATTATCATCGAGCCATGGCTTTGTTGGTATCTGGGTTGCTTTAACAATCTTCATGAGTTTGCGCACAGTGGTTGGATTGTGGAGGATAGGGACTGGAACTGGACCATGGAGCTTTCTGAGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

499

Amino Acids

53.58

Weight (kDa)

6.72

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 151 - 242 2.5e-12 MatE
MatE PF01554 302 - 454 6.9e-14 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1223
AasI GACNNNNNNGTC 1 cut(s) 595
Acc16I TGCGCA 1 cut(s) 1436
AciI CCGC 2 cut(s) 761, 1025
AclWI GGATC 2 cut(s) 970, 1089
AcoI YGGCCR 4 cut(s) 154, 630, 762, 868
AcsI RAATTY 2 cut(s) 189, 317
AcuI CTGAAG 1 cut(s) 288
AcvI CACGTG 1 cut(s) 1112
AdeI CACNNNGTG 1 cut(s) 605
AfaI GTAC 6 cut(s) 81, 295, 357, 406, 738, 1170
AfiI CCNNNNNNNGG 1 cut(s) 639
AflIII ACRYGT 1 cut(s) 1111
AgeI ACCGGT 1 cut(s) 755
AgsI TTSAA 6 cut(s) 31, 52, 208, 323, 494, 986
AjnI CCWGG 3 cut(s) 480, 844, 933
AluBI AGCT 9 cut(s) 56, 64, 175, 182, 914, 950, 1037, 1106, 1485
AluI AGCT 9 cut(s) 56, 64, 175, 182, 914, 950, 1037, 1106, 1485
Alw21I GWGCWC 3 cut(s) 177, 610, 1149
Alw26I GTCTC 1 cut(s) 571
AlwI GGATC 2 cut(s) 970, 1089
AlwNI CAGNNNCTG 2 cut(s) 122, 877
AoxI GGCC 4 cut(s) 154, 630, 762, 868
ApeKI GCWGC 9 cut(s) 125, 179, 474, 515, 914, 938, 950, 977, 1010
ApoI RAATTY 2 cut(s) 189, 317
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AseI ATTAAT 2 cut(s) 728, 1263
AsiGI ACCGGT 1 cut(s) 755
Asp700I GAANNNNTTC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 1437
AspS9I GGNCC 3 cut(s) 164, 718, 1475
AsuHPI GGTGA 2 cut(s) 913, 922
AvaII GGWCC 3 cut(s) 164, 718, 1475
BalI TGGCCA 3 cut(s) 156, 632, 870
BanII GRGCYC 1 cut(s) 177
BbrPI CACGTG 1 cut(s) 1112
Bbv12I GWGCWC 3 cut(s) 177, 610, 1149
BbvI GCAGC 9 cut(s) 112, 191, 486, 502, 901, 937, 950, 989, 1022
BccI CCATC 6 cut(s) 391, 454, 533, 1022, 1117, 1280
BciT130I CCWGG 3 cut(s) 482, 846, 935
BciVI GTATCC 1 cut(s) 655
BcoDI GTCTC 1 cut(s) 571
BfaI CTAG 3 cut(s) 65, 104, 1136
BfmI CTRYAG 1 cut(s) 612
BfuI GTATCC 1 cut(s) 655
BglII AGATCT 1 cut(s) 856
BmcAI AGTACT 1 cut(s) 295
Bme1390I CCNGG 3 cut(s) 482, 846, 935
Bme18I GGWCC 3 cut(s) 164, 718, 1475
BmgT120I GGNCC 3 cut(s) 164, 718, 1475
BmiI GGNNCC 2 cut(s) 285, 720
BmrFI CCNGG 3 cut(s) 482, 846, 935
BmsI GCATC 3 cut(s) 858, 950, 1313
BpuEI CTTGAG 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 1206
BsaAI YACGTR 1 cut(s) 1112
BsaBI GATNNNNATC 1 cut(s) 967
BsaJI CCNNGG 6 cut(s) 481, 649, 844, 933, 1387, 1478
BsaWI WCCGGW 1 cut(s) 755
BsaXI ACNNNNNCTCC 4 cut(s) 655, 685, 687, 717
Bsc4I CCNNNNNNNGG 1 cut(s) 639
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 4 cut(s) 167, 871, 1471, 1477
Bse3DI GCAATG 1 cut(s) 765
Bse8I GATNNNNATC 1 cut(s) 967
BseBI CCWGG 3 cut(s) 482, 846, 935
BseCI ATCGAT 1 cut(s) 1206
BseDI CCNNGG 6 cut(s) 481, 649, 844, 933, 1387, 1478
BseGI GGATG 4 cut(s) 386, 525, 1033, 1155
BseJI GATNNNNATC 1 cut(s) 967
BseLI CCNNNNNNNGG 1 cut(s) 639
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 4 cut(s) 797, 1164, 1268, 1481
BseNI ACTGG 4 cut(s) 167, 871, 1471, 1477
BseXI GCAGC 9 cut(s) 112, 191, 486, 502, 901, 937, 950, 989, 1022
BshFI GGCC 4 cut(s) 156, 632, 764, 870
BshTI ACCGGT 1 cut(s) 755
BshVI ATCGAT 1 cut(s) 1206
BsiHKAI GWGCWC 3 cut(s) 177, 610, 1149
BsiSI CCGG 1 cut(s) 756
BslFI GGGAC 1 cut(s) 1477
BslI CCNNNNNNNGG 1 cut(s) 639
BsmAI GTCTC 1 cut(s) 571
BsmFI GGGAC 1 cut(s) 1477
BsmI GAATGC 1 cut(s) 1233
BsnI GGCC 4 cut(s) 156, 632, 764, 870
Bsp1286I GDGCHC 3 cut(s) 177, 610, 1149
Bsp143I GATC 4 cut(s) 832, 856, 962, 1081
Bsp19I CCATGG 2 cut(s) 1387, 1478
BspACI CCGC 2 cut(s) 761, 1025
BspANI GGCC 4 cut(s) 156, 632, 764, 870
BspCNI CTCAG 4 cut(s) 796, 1163, 1267, 1482
BspDI ATCGAT 1 cut(s) 1206
BspHI TCATGA 1 cut(s) 1425
BspLI GGNNCC 2 cut(s) 285, 720
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 2 cut(s) 970, 1089
BsrDI GCAATG 1 cut(s) 765
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 4 cut(s) 167, 871, 1471, 1477
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 6 cut(s) 481, 649, 844, 933, 1387, 1478
BssMI GATC 4 cut(s) 832, 856, 962, 1081
BssT1I CCWWGG 2 cut(s) 1387, 1478
Bst2UI CCWGG 3 cut(s) 482, 846, 935
Bst4CI ACNGT 4 cut(s) 703, 807, 1165, 1441
BstAPI GCANNNNNTGC 4 cut(s) 122, 1058, 1322, 1362
BstBAI YACGTR 1 cut(s) 1112
BstC8I GCNNGC 4 cut(s) 177, 995, 1008, 1023
BstDEI CTNAG 5 cut(s) 593, 783, 1150, 1254, 1490
BstDSI CCRYGG 3 cut(s) 649, 1387, 1478
BstF5I GGATG 4 cut(s) 386, 525, 1033, 1155
BstHHI GCGC 1 cut(s) 1437
BstKTI GATC 4 cut(s) 835, 859, 965, 1084
BstMAI GTCTC 1 cut(s) 571
BstMBI GATC 4 cut(s) 832, 856, 962, 1081
BstNI CCWGG 3 cut(s) 482, 846, 935
BstNSI RCATGY 1 cut(s) 431
BstSCI CCNGG 3 cut(s) 480, 844, 933
BstSFI CTRYAG 1 cut(s) 612
BstV1I GCAGC 9 cut(s) 112, 191, 486, 502, 901, 937, 950, 989, 1022
BstX2I RGATCY 1 cut(s) 856
BstYI RGATCY 1 cut(s) 856
Bsu15I ATCGAT 1 cut(s) 1206
BsuI GTATCC 1 cut(s) 655
BsuRI GGCC 4 cut(s) 156, 632, 764, 870
BsuTUI ATCGAT 1 cut(s) 1206
BtgI CCRYGG 3 cut(s) 649, 1387, 1478
BtsCI GGATG 4 cut(s) 386, 525, 1033, 1155
BtsI GCAGTG 1 cut(s) 1254
BtsIMutI CAGTG 3 cut(s) 174, 1254, 1446
Cac8I GCNNGC 4 cut(s) 177, 995, 1008, 1023
CaiI CAGNNNCTG 2 cut(s) 122, 877
CciI TCATGA 1 cut(s) 1425
CfoI GCGC 1 cut(s) 1437
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 3 cut(s) 164, 718, 1475
ClaI ATCGAT 1 cut(s) 1206
CseI GACGC 2 cut(s) 466, 1170
Csp6I GTAC 6 cut(s) 80, 294, 356, 405, 737, 1169
CspAI ACCGGT 1 cut(s) 755
CviAII CATG 7 cut(s) 428, 538, 634, 773, 1388, 1426, 1479
CviQI GTAC 6 cut(s) 80, 294, 356, 405, 737, 1169
DdeI CTNAG 5 cut(s) 593, 783, 1150, 1254, 1490
DpnI GATC 4 cut(s) 834, 858, 964, 1083
DpnII GATC 4 cut(s) 832, 856, 962, 1081
DraIII CACNNNGTG 1 cut(s) 605
DrdI GACNNNNNNGTC 1 cut(s) 595
DseDI GACNNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 4 cut(s) 154, 630, 762, 868
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 2 cut(s) 1387, 1478
Eco24I GRGCYC 1 cut(s) 177
Eco32I GATATC 1 cut(s) 1204
Eco47I GGWCC 3 cut(s) 164, 718, 1475
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 288
Eco72I CACGTG 1 cut(s) 1112
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 480, 844, 933
EcoRV GATATC 1 cut(s) 1204
EcoT14I CCWWGG 2 cut(s) 1387, 1478
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 2 cut(s) 1387, 1478
FaeI CATG 7 cut(s) 431, 541, 637, 776, 1391, 1429, 1482
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 1 cut(s) 1477
FatI CATG 7 cut(s) 427, 537, 633, 772, 1387, 1425, 1478
FauNDI CATATG 1 cut(s) 744
FokI GGATG 4 cut(s) 373, 512, 1040, 1142
FriOI GRGCYC 1 cut(s) 177
FspBI CTAG 3 cut(s) 65, 104, 1136
FspI TGCGCA 1 cut(s) 1436
GlaI GCGC 1 cut(s) 1436
HaeIII GGCC 4 cut(s) 156, 632, 764, 870
HapII CCGG 1 cut(s) 756
HgaI GACGC 2 cut(s) 466, 1170
HhaI GCGC 1 cut(s) 1437
Hin1II CATG 7 cut(s) 431, 541, 637, 776, 1391, 1429, 1482
Hin6I GCGC 1 cut(s) 1435
HinP1I GCGC 1 cut(s) 1435
HincII GTYRAC 2 cut(s) 340, 373
HindII GTYRAC 2 cut(s) 340, 373
HinfI GANTC 3 cut(s) 374, 548, 1252
HpaII CCGG 1 cut(s) 756
HphI GGTGA 2 cut(s) 913, 922
Hpy166II GTNNAC 3 cut(s) 340, 373, 706
Hpy188I TCNGA 4 cut(s) 434, 879, 1309, 1491
Hpy188III TCNNGA 2 cut(s) 1185, 1426
Hpy8I GTNNAC 3 cut(s) 340, 373, 706
HpyAV CCTTC 2 cut(s) 429, 1072
HpyCH4III ACNGT 4 cut(s) 703, 807, 1165, 1441
HpyCH4IV ACGT 2 cut(s) 354, 1111
HpyF3I CTNAG 5 cut(s) 593, 783, 1150, 1254, 1490
HpySE526I ACGT 2 cut(s) 354, 1111
Hsp92II CATG 7 cut(s) 431, 541, 637, 776, 1391, 1429, 1482
HspAI GCGC 1 cut(s) 1435
Kzo9I GATC 4 cut(s) 832, 856, 962, 1081
LmnI GCTCC 4 cut(s) 172, 613, 1301, 1482
Lsp1109I GCAGC 9 cut(s) 112, 191, 486, 502, 901, 937, 950, 989, 1022
LweI GCATC 3 cut(s) 858, 950, 1313
MaeI CTAG 3 cut(s) 65, 104, 1136
MaeII ACGT 2 cut(s) 354, 1111
MaeIII GTNAC 3 cut(s) 597, 928, 1249
MalI GATC 4 cut(s) 834, 858, 964, 1083
MboI GATC 4 cut(s) 832, 856, 962, 1081
MboII GAAGA 6 cut(s) 19, 281, 284, 326, 611, 1414
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 1 cut(s) 856
MhlI GDGCHC 3 cut(s) 177, 610, 1149
MlsI TGGCCA 3 cut(s) 156, 632, 870
MluCI AATT 9 cut(s) 135, 189, 228, 317, 532, 553, 687, 864, 1294
MluNI TGGCCA 3 cut(s) 156, 632, 870
MlyI GAGTC 2 cut(s) 368, 1246
MmeI TCCRAC 1 cut(s) 1426
MnlI CCTC 5 cut(s) 26, 265, 1024, 1449, 1485
Mox20I TGGCCA 3 cut(s) 156, 632, 870
MroXI GAANNNNTTC 1 cut(s) 47
MscI TGGCCA 3 cut(s) 156, 632, 870
MseI TTAA 8 cut(s) 290, 566, 657, 728, 791, 818, 1263, 1415
Msp20I TGGCCA 3 cut(s) 156, 632, 870
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 1 cut(s) 756
MspR9I CCNGG 3 cut(s) 482, 846, 935
MunI CAATTG 1 cut(s) 687
Mva1269I GAATGC 1 cut(s) 1233
MvaI CCWGG 3 cut(s) 482, 846, 935
NcoI CCATGG 2 cut(s) 1387, 1478
NdeI CATATG 1 cut(s) 744
NdeII GATC 4 cut(s) 832, 856, 962, 1081
NlaIII CATG 7 cut(s) 431, 541, 637, 776, 1391, 1429, 1482
NlaIV GGNNCC 2 cut(s) 285, 720
NmuCI GTSAC 3 cut(s) 597, 928, 1249
NsbI TGCGCA 1 cut(s) 1436
NspI RCATGY 1 cut(s) 431
PagI TCATGA 1 cut(s) 1425
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PctI GAATGC 1 cut(s) 1233
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 1 cut(s) 548
PinAI ACCGGT 1 cut(s) 755
PleI GAGTC 2 cut(s) 368, 1246
PmaCI CACGTG 1 cut(s) 1112
PmlI CACGTG 1 cut(s) 1112
PpsI GAGTC 2 cut(s) 368, 1246
Ppu21I YACGTR 1 cut(s) 1112
PshBI ATTAAT 2 cut(s) 728, 1263
PsiI TTATAA 1 cut(s) 1223
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 3 cut(s) 480, 844, 933
PspCI CACGTG 1 cut(s) 1112
PspGI CCWGG 3 cut(s) 480, 844, 933
PspN4I GGNNCC 2 cut(s) 285, 720
PspPI GGNCC 3 cut(s) 164, 718, 1475
PstI CTGCAG 1 cut(s) 616
PstNI CAGNNNCTG 2 cut(s) 122, 877
PsuI RGATCY 1 cut(s) 856
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 6 cut(s) 81, 295, 357, 406, 738, 1170
RsaNI GTAC 6 cut(s) 80, 294, 356, 405, 737, 1169
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 8 cut(s) 290, 566, 657, 728, 791, 818, 1263, 1415
Sau3AI GATC 4 cut(s) 832, 856, 962, 1081
Sau96I GGNCC 3 cut(s) 164, 718, 1475
ScaI AGTACT 1 cut(s) 295
SchI GAGTC 2 cut(s) 368, 1246
ScrFI CCNGG 3 cut(s) 482, 846, 935
SduI GDGCHC 3 cut(s) 177, 610, 1149
SfaNI GCATC 3 cut(s) 858, 950, 1313
SfcI CTRYAG 1 cut(s) 612
SinI GGWCC 3 cut(s) 164, 718, 1475
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 9 cut(s) 135, 189, 228, 317, 532, 553, 687, 864, 1294
SsiI CCGC 2 cut(s) 761, 1025
SspI AATATT 1 cut(s) 726
SspMI CTAG 3 cut(s) 65, 104, 1136
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 3 cut(s) 480, 844, 933
StyI CCWWGG 2 cut(s) 1387, 1478
TaaI ACNGT 4 cut(s) 703, 807, 1165, 1441
TaiI ACGT 2 cut(s) 357, 1114
TaqI TCGA 3 cut(s) 965, 1206, 1382
TasI AATT 9 cut(s) 135, 189, 228, 317, 532, 553, 687, 864, 1294
TatI WGTACW 5 cut(s) 79, 293, 404, 736, 1168
TauI GCSGC 1 cut(s) 764
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 8 cut(s) 290, 566, 657, 728, 791, 818, 1263, 1415
Tru9I TTAA 8 cut(s) 290, 566, 657, 728, 791, 818, 1263, 1415
TscAI CASTG 3 cut(s) 174, 1254, 1446
TseFI GTSAC 3 cut(s) 597, 928, 1249
TseI GCWGC 9 cut(s) 125, 179, 474, 515, 914, 938, 950, 977, 1010
Tsp45I GTSAC 3 cut(s) 597, 928, 1249
TspDTI ATGAA 3 cut(s) 327, 444, 1414
TspGWI ACGGA 3 cut(s) 296, 638, 1227
TspRI CASTG 3 cut(s) 174, 1254, 1446
VpaK11BI GGWCC 3 cut(s) 164, 718, 1475
VspI ATTAAT 2 cut(s) 728, 1263
XapI RAATTY 2 cut(s) 189, 317
XceI RCATGY 1 cut(s) 431
XcmI CCANNNNNNNNNTGG 2 cut(s) 1267, 1394
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 3 cut(s) 65, 104, 1136
ZrmI AGTACT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.