Rmu_sc0005445.1_g000002

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005445.1
OG0001107
Physical Location & Seq
Reverse (-)
2326 .. 9898
7573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005445.1_g000002.1.cds

Sequence Viewer

Length: 1608 bp
atgggcaagatgcccctccttgtgttcttcaaagacacaaggaatgttttcaaagctgatgagctaggcgtggagatagtacatattgcacttcctacgctactagcgttagtagcagaccctgctgcttctctaattgacacagcgtttgttggccatataggtccagtggagctcgcagctgtgggaatttctattgctgttttcaatcaagtatcaaaaatagcaattttcccacttgtcagtattacaacctcttttgttgctgaagaagatactacggaaccattacgtactaaagaacaagtatatgaagaatttgaaaatgctacttctgtcaacaaggaaatggaagtactcaaaccacttgttgactctacaccatcatccatttccaatgatagtacagaaatggtcaaacttgagcatgtcagaaggcgtattccatctgcttcatcagcattggttattggcagcgtcctgggggttattcaaactctattccttatttttgctgcaaaccccatcctaaattacatgggggtagattctaattctcctatgttaagaccagcgagacaatacctgacactaaggtcacttggtgctcctgcagttcttctctctttggccatgcaaggggttttccgtggttttaaggatacaaaaactcctttatatgctacaattgtgggagatacagtaaacatcattttggacccaatattaatgtttgtactccatatgggcattaccggtgcggccattgcccatgttatctctcagtatttaatctccctgatactgttatggagattaaacaaacaagttgatctcctaaccccaggcatcaaagatctacgaattggccagtttctgaaaaatgggggtatgttattggtgagagttatagctgcaacattctgtgtcaccctggcagcatcattagctgcaaggcagggatcgacatcaatggcagcatttcaagtttgcttgcagatttggcttgcagcctctttgcttgcggatgggttagctgtggctggacaaatttggcttgcagcctctttgcttgcggatgggttagctgtggctggacaagcaatccttgcaagtgcatttgcaaggaaggatcacagcaaggtcgttgccacagcttcacgtgtattgcagatggctttggttctagggatagtgctctcagtcatccttacagttgtactaaagtttgcgtcaagattatttaccgatgatatcgatgttttgcaccttataagtttaggcattccgtttattgcagtgactcagcccattaatgctctggcatttgtttttgatggagtaaattatggagcatcagattttgcatattctgcctactcaatggcaagtgtagaaatccatgttgctgtctatttttcacttgtatactgtgttggtggctttagtgagcatattgtgcctgtttctattatcatcgagccatggctttgttggtatctgggttgctttaacaatcttcatgagtttgcgcacagtggttggattttggaggatagggactggaactggaccatggagctttctgagggagtaattgaagcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

535

Amino Acids

57.83

Weight (kDa)

5.39

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1274
AasI GACNNNNNNGTC 1 cut(s) 595
Acc16I TGCGCA 1 cut(s) 1533
AccI GTMKAC 1 cut(s) 1428
AciI CCGC 3 cut(s) 761, 1025, 1076
AclWI GGATC 2 cut(s) 970, 1140
AcoI YGGCCR 4 cut(s) 154, 630, 762, 868
AcsI RAATTY 3 cut(s) 189, 317, 1050
AcuI CTGAAG 1 cut(s) 288
AcvI CACGTG 1 cut(s) 1163
AdeI CACNNNGTG 1 cut(s) 605
AfaI GTAC 6 cut(s) 81, 295, 357, 406, 738, 1221
AfiI CCNNNNNNNGG 1 cut(s) 639
AflIII ACRYGT 1 cut(s) 1162
AgeI ACCGGT 1 cut(s) 755
AgsI TTSAA 7 cut(s) 31, 52, 208, 323, 494, 986, 1601
AjnI CCWGG 3 cut(s) 480, 844, 933
Alw21I GWGCWC 3 cut(s) 177, 610, 1200
Alw26I GTCTC 1 cut(s) 571
AlwI GGATC 2 cut(s) 970, 1140
AlwNI CAGNNNCTG 2 cut(s) 122, 877
AoxI GGCC 4 cut(s) 154, 630, 762, 868
ApoI RAATTY 3 cut(s) 189, 317, 1050
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AseI ATTAAT 2 cut(s) 728, 1314
AsiGI ACCGGT 1 cut(s) 755
Asp700I GAANNNNTTC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 1534
AspS9I GGNCC 3 cut(s) 164, 718, 1572
AsuHPI GGTGA 2 cut(s) 913, 922
AvaII GGWCC 3 cut(s) 164, 718, 1572
BalI TGGCCA 3 cut(s) 156, 632, 870
BanII GRGCYC 1 cut(s) 177
BbrPI CACGTG 1 cut(s) 1163
Bbv12I GWGCWC 3 cut(s) 177, 610, 1200
BccI CCATC 7 cut(s) 391, 454, 533, 1022, 1073, 1168, 1331
BciT130I CCWGG 3 cut(s) 482, 846, 935
BciVI GTATCC 1 cut(s) 655
BcoDI GTCTC 1 cut(s) 571
BfaI CTAG 3 cut(s) 65, 104, 1187
BfmI CTRYAG 1 cut(s) 612
BfuI GTATCC 1 cut(s) 655
BglII AGATCT 1 cut(s) 856
BmcAI AGTACT 1 cut(s) 357
Bme1390I CCNGG 3 cut(s) 482, 846, 935
Bme18I GGWCC 3 cut(s) 164, 718, 1572
BmgT120I GGNCC 3 cut(s) 164, 718, 1572
BmiI GGNNCC 2 cut(s) 285, 720
BmrFI CCNGG 3 cut(s) 482, 846, 935
BmsI GCATC 3 cut(s) 858, 950, 1364
BpuEI CTTGAG 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 1257
BsaAI YACGTR 2 cut(s) 293, 1163
BsaBI GATNNNNATC 1 cut(s) 967
BsaJI CCNNGG 6 cut(s) 481, 649, 844, 933, 1484, 1575
BsaWI WCCGGW 1 cut(s) 755
BsaXI ACNNNNNCTCC 4 cut(s) 655, 685, 687, 717
Bsc4I CCNNNNNNNGG 1 cut(s) 639
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 4 cut(s) 167, 871, 1568, 1574
Bse3DI GCAATG 1 cut(s) 765
Bse8I GATNNNNATC 1 cut(s) 967
BseBI CCWGG 3 cut(s) 482, 846, 935
BseCI ATCGAT 1 cut(s) 1257
BseDI CCNNGG 6 cut(s) 481, 649, 844, 933, 1484, 1575
BseGI GGATG 5 cut(s) 386, 525, 1033, 1084, 1206
BseJI GATNNNNATC 1 cut(s) 967
BseLI CCNNNNNNNGG 1 cut(s) 639
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 4 cut(s) 797, 1215, 1319, 1578
BseNI ACTGG 4 cut(s) 167, 871, 1568, 1574
BshFI GGCC 4 cut(s) 156, 632, 764, 870
BshTI ACCGGT 1 cut(s) 755
BshVI ATCGAT 1 cut(s) 1257
BsiHKAI GWGCWC 3 cut(s) 177, 610, 1200
BsiSI CCGG 1 cut(s) 756
BslFI GGGAC 1 cut(s) 1574
BslI CCNNNNNNNGG 1 cut(s) 639
BsmAI GTCTC 1 cut(s) 571
BsmFI GGGAC 1 cut(s) 1574
BsmI GAATGC 1 cut(s) 1284
BsnI GGCC 4 cut(s) 156, 632, 764, 870
Bsp1286I GDGCHC 3 cut(s) 177, 610, 1200
Bsp143I GATC 4 cut(s) 832, 856, 962, 1132
Bsp19I CCATGG 2 cut(s) 1484, 1575
BspACI CCGC 3 cut(s) 761, 1025, 1076
BspANI GGCC 4 cut(s) 156, 632, 764, 870
BspCNI CTCAG 4 cut(s) 796, 1214, 1318, 1579
BspDI ATCGAT 1 cut(s) 1257
BspHI TCATGA 1 cut(s) 1522
BspLI GGNNCC 2 cut(s) 285, 720
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 2 cut(s) 970, 1140
BsrDI GCAATG 1 cut(s) 765
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 4 cut(s) 167, 871, 1568, 1574
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 6 cut(s) 481, 649, 844, 933, 1484, 1575
BssMI GATC 4 cut(s) 832, 856, 962, 1132
BssNAI GTATAC 1 cut(s) 1429
BssT1I CCWWGG 2 cut(s) 1484, 1575
Bst1107I GTATAC 1 cut(s) 1429
Bst2UI CCWGG 3 cut(s) 482, 846, 935
Bst4CI ACNGT 5 cut(s) 703, 807, 1216, 1433, 1538
BstAPI GCANNNNNTGC 4 cut(s) 122, 1109, 1373, 1459
BstBAI YACGTR 2 cut(s) 293, 1163
BstC8I GCNNGC 6 cut(s) 177, 995, 1008, 1023, 1059, 1074
BstDEI CTNAG 5 cut(s) 593, 783, 1201, 1305, 1587
BstDSI CCRYGG 3 cut(s) 649, 1484, 1575
BstF5I GGATG 5 cut(s) 386, 525, 1033, 1084, 1206
BstHHI GCGC 1 cut(s) 1534
BstKTI GATC 4 cut(s) 835, 859, 965, 1135
BstMAI GTCTC 1 cut(s) 571
BstMBI GATC 4 cut(s) 832, 856, 962, 1132
BstNI CCWGG 3 cut(s) 482, 846, 935
BstNSI RCATGY 1 cut(s) 431
BstSCI CCNGG 3 cut(s) 480, 844, 933
BstSFI CTRYAG 1 cut(s) 612
BstSNI TACGTA 1 cut(s) 293
BstX2I RGATCY 1 cut(s) 856
BstYI RGATCY 1 cut(s) 856
BstZ17I GTATAC 1 cut(s) 1429
Bsu15I ATCGAT 1 cut(s) 1257
BsuI GTATCC 1 cut(s) 655
BsuRI GGCC 4 cut(s) 156, 632, 764, 870
BsuTUI ATCGAT 1 cut(s) 1257
BtgI CCRYGG 3 cut(s) 649, 1484, 1575
BtsCI GGATG 5 cut(s) 386, 525, 1033, 1084, 1206
BtsI GCAGTG 1 cut(s) 1305
BtsIMutI CAGTG 3 cut(s) 174, 1305, 1543
Cac8I GCNNGC 6 cut(s) 177, 995, 1008, 1023, 1059, 1074
CaiI CAGNNNCTG 2 cut(s) 122, 877
CciI TCATGA 1 cut(s) 1522
CfoI GCGC 1 cut(s) 1534
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 3 cut(s) 164, 718, 1572
ClaI ATCGAT 1 cut(s) 1257
CseI GACGC 2 cut(s) 466, 1221
Csp6I GTAC 6 cut(s) 80, 294, 356, 405, 737, 1220
CspAI ACCGGT 1 cut(s) 755
CviAII CATG 8 cut(s) 428, 538, 634, 773, 1403, 1485, 1523, 1576
CviQI GTAC 6 cut(s) 80, 294, 356, 405, 737, 1220
DdeI CTNAG 5 cut(s) 593, 783, 1201, 1305, 1587
DpnI GATC 4 cut(s) 834, 858, 964, 1134
DpnII GATC 4 cut(s) 832, 856, 962, 1132
DraIII CACNNNGTG 1 cut(s) 605
DrdI GACNNNNNNGTC 1 cut(s) 595
DseDI GACNNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 4 cut(s) 154, 630, 762, 868
Ecl136II GAGCTC 1 cut(s) 175
Eco105I TACGTA 1 cut(s) 293
Eco130I CCWWGG 2 cut(s) 1484, 1575
Eco24I GRGCYC 1 cut(s) 177
Eco32I GATATC 1 cut(s) 1255
Eco47I GGWCC 3 cut(s) 164, 718, 1572
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 288
Eco72I CACGTG 1 cut(s) 1163
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 480, 844, 933
EcoRV GATATC 1 cut(s) 1255
EcoT14I CCWWGG 2 cut(s) 1484, 1575
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 2 cut(s) 1484, 1575
FaeI CATG 8 cut(s) 431, 541, 637, 776, 1406, 1488, 1526, 1579
FalI AAGNNNNNCTT 2 cut(s) 1092, 1124
FaqI GGGAC 1 cut(s) 1574
FatI CATG 8 cut(s) 427, 537, 633, 772, 1402, 1484, 1522, 1575
FauNDI CATATG 1 cut(s) 744
FblI GTMKAC 1 cut(s) 1428
FokI GGATG 5 cut(s) 373, 512, 1040, 1091, 1193
FriOI GRGCYC 1 cut(s) 177
FspBI CTAG 3 cut(s) 65, 104, 1187
FspI TGCGCA 1 cut(s) 1533
GlaI GCGC 1 cut(s) 1533
HaeIII GGCC 4 cut(s) 156, 632, 764, 870
HapII CCGG 1 cut(s) 756
HgaI GACGC 2 cut(s) 466, 1221
HhaI GCGC 1 cut(s) 1534
Hin1II CATG 8 cut(s) 431, 541, 637, 776, 1406, 1488, 1526, 1579
Hin6I GCGC 1 cut(s) 1532
HinP1I GCGC 1 cut(s) 1532
HincII GTYRAC 2 cut(s) 340, 373
HindII GTYRAC 2 cut(s) 340, 373
HinfI GANTC 3 cut(s) 374, 548, 1303
HpaII CCGG 1 cut(s) 756
HphI GGTGA 2 cut(s) 913, 922
Hpy166II GTNNAC 4 cut(s) 340, 373, 706, 1429
Hpy188I TCNGA 4 cut(s) 434, 879, 1360, 1588
Hpy188III TCNNGA 2 cut(s) 1236, 1523
Hpy8I GTNNAC 4 cut(s) 340, 373, 706, 1429
HpyAV CCTTC 2 cut(s) 429, 1123
HpyCH4III ACNGT 5 cut(s) 703, 807, 1216, 1433, 1538
HpyCH4IV ACGT 2 cut(s) 292, 1162
HpyF3I CTNAG 5 cut(s) 593, 783, 1201, 1305, 1587
HpySE526I ACGT 2 cut(s) 292, 1162
Hsp92II CATG 8 cut(s) 431, 541, 637, 776, 1406, 1488, 1526, 1579
HspAI GCGC 1 cut(s) 1532
Kzo9I GATC 4 cut(s) 832, 856, 962, 1132
LmnI GCTCC 4 cut(s) 172, 613, 1352, 1579
LweI GCATC 3 cut(s) 858, 950, 1364
MaeI CTAG 3 cut(s) 65, 104, 1187
MaeII ACGT 2 cut(s) 292, 1162
MaeIII GTNAC 3 cut(s) 597, 928, 1300
MalI GATC 4 cut(s) 834, 858, 964, 1134
MboI GATC 4 cut(s) 832, 856, 962, 1132
MboII GAAGA 6 cut(s) 19, 281, 284, 326, 611, 1511
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 1 cut(s) 856
MhlI GDGCHC 3 cut(s) 177, 610, 1200
MlsI TGGCCA 3 cut(s) 156, 632, 870
MluNI TGGCCA 3 cut(s) 156, 632, 870
MlyI GAGTC 2 cut(s) 368, 1297
MmeI TCCRAC 1 cut(s) 1523
MnlI CCTC 6 cut(s) 26, 265, 1024, 1075, 1546, 1582
Mox20I TGGCCA 3 cut(s) 156, 632, 870
MroXI GAANNNNTTC 1 cut(s) 47
MscI TGGCCA 3 cut(s) 156, 632, 870
MseI TTAA 7 cut(s) 566, 657, 728, 791, 818, 1314, 1512
Msp20I TGGCCA 3 cut(s) 156, 632, 870
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 1 cut(s) 756
MspR9I CCNGG 3 cut(s) 482, 846, 935
MunI CAATTG 1 cut(s) 687
Mva1269I GAATGC 1 cut(s) 1284
MvaI CCWGG 3 cut(s) 482, 846, 935
NcoI CCATGG 2 cut(s) 1484, 1575
NdeI CATATG 1 cut(s) 744
NdeII GATC 4 cut(s) 832, 856, 962, 1132
NlaIII CATG 8 cut(s) 431, 541, 637, 776, 1406, 1488, 1526, 1579
NlaIV GGNNCC 2 cut(s) 285, 720
NmuCI GTSAC 3 cut(s) 597, 928, 1300
NsbI TGCGCA 1 cut(s) 1533
NspI RCATGY 1 cut(s) 431
PagI TCATGA 1 cut(s) 1522
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PctI GAATGC 1 cut(s) 1284
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 1 cut(s) 548
PinAI ACCGGT 1 cut(s) 755
PleI GAGTC 2 cut(s) 368, 1297
PmaCI CACGTG 1 cut(s) 1163
PmlI CACGTG 1 cut(s) 1163
PpsI GAGTC 2 cut(s) 368, 1297
Ppu21I YACGTR 2 cut(s) 293, 1163
PshBI ATTAAT 2 cut(s) 728, 1314
PsiI TTATAA 1 cut(s) 1274
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 3 cut(s) 480, 844, 933
PspCI CACGTG 1 cut(s) 1163
PspGI CCWGG 3 cut(s) 480, 844, 933
PspN4I GGNNCC 2 cut(s) 285, 720
PspPI GGNCC 3 cut(s) 164, 718, 1572
PstI CTGCAG 1 cut(s) 616
PstNI CAGNNNCTG 2 cut(s) 122, 877
PsuI RGATCY 1 cut(s) 856
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 6 cut(s) 81, 295, 357, 406, 738, 1221
RsaNI GTAC 6 cut(s) 80, 294, 356, 405, 737, 1220
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 7 cut(s) 566, 657, 728, 791, 818, 1314, 1512
Sau3AI GATC 4 cut(s) 832, 856, 962, 1132
Sau96I GGNCC 3 cut(s) 164, 718, 1572
ScaI AGTACT 1 cut(s) 357
SchI GAGTC 2 cut(s) 368, 1297
ScrFI CCNGG 3 cut(s) 482, 846, 935
SduI GDGCHC 3 cut(s) 177, 610, 1200
SfaNI GCATC 3 cut(s) 858, 950, 1364
SfcI CTRYAG 1 cut(s) 612
SinI GGWCC 3 cut(s) 164, 718, 1572
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SnaBI TACGTA 1 cut(s) 293
SsiI CCGC 3 cut(s) 761, 1025, 1076
SspI AATATT 1 cut(s) 726
SspMI CTAG 3 cut(s) 65, 104, 1187
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 3 cut(s) 480, 844, 933
StyI CCWWGG 2 cut(s) 1484, 1575
TaaI ACNGT 5 cut(s) 703, 807, 1216, 1433, 1538
TaiI ACGT 2 cut(s) 295, 1165
TaqI TCGA 3 cut(s) 965, 1257, 1479
TatI WGTACW 5 cut(s) 79, 355, 404, 736, 1219
TauI GCSGC 1 cut(s) 764
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 7 cut(s) 566, 657, 728, 791, 818, 1314, 1512
Tru9I TTAA 7 cut(s) 566, 657, 728, 791, 818, 1314, 1512
TscAI CASTG 3 cut(s) 174, 1305, 1543
TseFI GTSAC 3 cut(s) 597, 928, 1300
Tsp45I GTSAC 3 cut(s) 597, 928, 1300
TspDTI ATGAA 3 cut(s) 327, 444, 1511
TspGWI ACGGA 3 cut(s) 296, 638, 1278
TspRI CASTG 3 cut(s) 174, 1305, 1543
VpaK11BI GGWCC 3 cut(s) 164, 718, 1572
VspI ATTAAT 2 cut(s) 728, 1314
XapI RAATTY 3 cut(s) 189, 317, 1050
XceI RCATGY 1 cut(s) 431
XcmI CCANNNNNNNNNTGG 2 cut(s) 1318, 1491
XmiI GTMKAC 1 cut(s) 1428
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 3 cut(s) 65, 104, 1187
ZrmI AGTACT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.