Rh3BG277400

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001107
Physical Location & Seq
Forward (+)
27188477 .. 27196488
8012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG277400.1

Sequence Viewer

Length: 1152 bp
ATGGAAGTACTCAAACCACTTGTTGACTCTACACCATCATCCATTACCAATGATAGTACAGAAATGGTCAAACTTGAGCATGTCAGAAGGCGTATTCCGTCTGCTTCATCAGCATTGGTTATTGGCAGCGTCCTGGGGGTTATTCAAACTCTATTCCTAATTTTTGCTGCAAACCCCATCCTAAATTACATGGGGGTAGATTCTAATTCTCCTATGTTAAGACCAGCGAGACAATACCTGACACTAAGGGCGCTTGGTGCTCCTGCAGTTCTTCTCTCTTTGGCCATGCAAGGGGTTTTCCGTGGTTTTAAGGATACGAAAACTCCTTTATATGCTACAATTGTGGGAGATACAGTAAACATCATTTTGGACCCAATATTAATGTTTGTACTCCATATGGGCATTACCGGTGCAGCCATTGCCCATGTTATCTCTCAGTATTTAATCTCCCTGATACTGTTATGGAGATTAAACAAACAAGTTGATCTCCTAACCCCAGGCATCAAAGATCTACGAATTGGCCAGTTTCTGAAAAATGGGGGTATGTTATTGGTGAGAGTTATAGCTGCAACATTCTGTGTCACCCTGGCAGCATCATTAGCTGCAAGGCAGGGATCGACATCAATGGCAGCATTTCAAGTTTGCTTGCAGATTTGGCTTGCAGCCTCTTTGCTTGCGGATGGGTTAGCTGTGGCTGGACAAGCAATCCTTGCAAGTGCATTTGCGAGGAAGGATCACAGCAAGGTCGTTGCCACAGCTTCACGTGTATTGCAGATGGCTTTGGTTCTAGGGATAGTGCTCTCAGTCATCCTTACAGTTGTACTAAAGTTCGCGTCAAGATTATTTACCGATGATATCAATGTTTTGCACCTTATAAGTTTAGGCATTCCGTTTATTGCATTGACTCAGCCCGTTAATGCTCTGGCATTTGTTTTTGATGGAGTAAATTATGGAGCATCAGATTTTGCATATTCTGCCTACTCAATGGTGTTGGTGGCTTTAGTGAGCATATTGTGCCTGTTTCTATTATCATCAAGCCATGGCTTTGTTGGTATCTGGGTTGCTTTAACAATCTTCATGAGTTTGCGCACAGTGGTTGGATTTTGGAGGATAGGCACTGGAACTGGACCATGGAGCTTTCTGAGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

383

Amino Acids

41.16

Weight (kDa)

9.67

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 35 - 126 4.3e-13 MatE
MatE PF01554 186 - 338 1e-13 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 875
Acc16I TGCGCA 1 cut(s) 1088
AccII CGCG 1 cut(s) 833
AciI CCGC 1 cut(s) 677
AclWI GGATC 2 cut(s) 622, 741
AcoI YGGCCR 2 cut(s) 282, 520
AcvI CACGTG 1 cut(s) 764
AfaI GTAC 4 cut(s) 9, 58, 390, 822
AfiI CCNNNNNNNGG 1 cut(s) 291
AflIII ACRYGT 1 cut(s) 763
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 2 cut(s) 146, 638
AjnI CCWGG 3 cut(s) 132, 496, 585
AluBI AGCT 5 cut(s) 566, 602, 689, 758, 1137
AluI AGCT 5 cut(s) 566, 602, 689, 758, 1137
Alw21I GWGCWC 2 cut(s) 262, 801
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 2 cut(s) 622, 741
AlwNI CAGNNNCTG 1 cut(s) 529
AoxI GGCC 2 cut(s) 282, 520
ApeKI GCWGC 8 cut(s) 126, 167, 413, 566, 590, 602, 629, 662
AseI ATTAAT 1 cut(s) 380
AsiGI ACCGGT 1 cut(s) 407
AspLEI GCGC 2 cut(s) 253, 1089
AspS9I GGNCC 2 cut(s) 370, 1127
AsuHPI GGTGA 2 cut(s) 565, 574
AvaII GGWCC 2 cut(s) 370, 1127
BalI TGGCCA 2 cut(s) 284, 522
BbrPI CACGTG 1 cut(s) 764
Bbv12I GWGCWC 2 cut(s) 262, 801
BbvI GCAGC 8 cut(s) 138, 154, 425, 553, 589, 602, 641, 674
BccI CCATC 5 cut(s) 43, 185, 674, 769, 932
BciT130I CCWGG 3 cut(s) 134, 498, 587
BciVI GTATCC 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 1 cut(s) 788
BfmI CTRYAG 1 cut(s) 264
BfoI RGCGCY 1 cut(s) 254
BfuI GTATCC 1 cut(s) 307
BglII AGATCT 1 cut(s) 508
BisI GCNGC 8 cut(s) 127, 168, 414, 567, 591, 603, 630, 663
BlsI GCNGC 8 cut(s) 128, 169, 415, 568, 592, 604, 631, 664
BmcAI AGTACT 1 cut(s) 9
Bme1390I CCNGG 3 cut(s) 134, 498, 587
Bme18I GGWCC 2 cut(s) 370, 1127
BmgT120I GGNCC 2 cut(s) 370, 1127
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 3 cut(s) 134, 498, 587
BmsI GCATC 3 cut(s) 510, 602, 965
BpuEI CTTGAG 1 cut(s) 95
BsaAI YACGTR 1 cut(s) 764
BsaBI GATNNNNATC 1 cut(s) 619
BsaJI CCNNGG 6 cut(s) 133, 301, 496, 585, 1039, 1130
BsaWI WCCGGW 1 cut(s) 407
BsaXI ACNNNNNCTCC 4 cut(s) 307, 337, 339, 369
Bsc4I CCNNNNNNNGG 1 cut(s) 291
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 3 cut(s) 523, 1123, 1129
Bse3DI GCAATG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 619
BseBI CCWGG 3 cut(s) 134, 498, 587
BseDI CCNNGG 6 cut(s) 133, 301, 496, 585, 1039, 1130
BseGI GGATG 4 cut(s) 38, 177, 685, 807
BseJI GATNNNNATC 1 cut(s) 619
BseLI CCNNNNNNNGG 1 cut(s) 291
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 4 cut(s) 449, 816, 920, 1133
BseNI ACTGG 3 cut(s) 523, 1123, 1129
BseXI GCAGC 8 cut(s) 138, 154, 425, 553, 589, 602, 641, 674
BsgI GTGCAG 1 cut(s) 432
Bsh1236I CGCG 1 cut(s) 833
BshFI GGCC 2 cut(s) 284, 522
BshTI ACCGGT 1 cut(s) 407
BsiHKAI GWGCWC 2 cut(s) 262, 801
BsiSI CCGG 1 cut(s) 408
BslI CCNNNNNNNGG 1 cut(s) 291
BsmAI GTCTC 1 cut(s) 223
BsmI GAATGC 1 cut(s) 885
BsnI GGCC 2 cut(s) 284, 522
Bsp1286I GDGCHC 2 cut(s) 262, 801
Bsp143I GATC 4 cut(s) 484, 508, 614, 733
Bsp19I CCATGG 2 cut(s) 1039, 1130
BspACI CCGC 1 cut(s) 677
BspANI GGCC 2 cut(s) 284, 522
BspCNI CTCAG 4 cut(s) 448, 815, 919, 1134
BspFNI CGCG 1 cut(s) 833
BspHI TCATGA 1 cut(s) 1077
BspLI GGNNCC 1 cut(s) 372
BspMAI CTGCAG 1 cut(s) 268
BspPI GGATC 2 cut(s) 622, 741
BsrDI GCAATG 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 407
BsrI ACTGG 3 cut(s) 523, 1123, 1129
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 6 cut(s) 133, 301, 496, 585, 1039, 1130
BssMI GATC 4 cut(s) 484, 508, 614, 733
BssT1I CCWWGG 2 cut(s) 1039, 1130
Bst2UI CCWGG 3 cut(s) 134, 498, 587
Bst4CI ACNGT 4 cut(s) 355, 459, 817, 1093
BstAPI GCANNNNNTGC 4 cut(s) 419, 710, 974, 1014
BstBAI YACGTR 1 cut(s) 764
BstC8I GCNNGC 3 cut(s) 647, 660, 675
BstDEI CTNAG 5 cut(s) 245, 435, 802, 906, 1142
BstDSI CCRYGG 3 cut(s) 301, 1039, 1130
BstF5I GGATG 4 cut(s) 38, 177, 685, 807
BstFNI CGCG 1 cut(s) 833
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 2 cut(s) 253, 1089
BstKTI GATC 4 cut(s) 487, 511, 617, 736
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 4 cut(s) 484, 508, 614, 733
BstMWI GCNNNNNNNGC 9 cut(s) 110, 257, 419, 599, 655, 701, 710, 974, 1014
BstNI CCWGG 3 cut(s) 134, 498, 587
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 3 cut(s) 132, 496, 585
BstSFI CTRYAG 1 cut(s) 264
BstUI CGCG 1 cut(s) 833
BstV1I GCAGC 8 cut(s) 138, 154, 425, 553, 589, 602, 641, 674
BstX2I RGATCY 1 cut(s) 508
BstYI RGATCY 1 cut(s) 508
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 2 cut(s) 284, 522
BtgI CCRYGG 3 cut(s) 301, 1039, 1130
BtsCI GGATG 4 cut(s) 38, 177, 685, 807
BtsIMutI CAGTG 2 cut(s) 1098, 1116
Cac8I GCNNGC 3 cut(s) 647, 660, 675
CaiI CAGNNNCTG 1 cut(s) 529
CciI TCATGA 1 cut(s) 1077
CfoI GCGC 2 cut(s) 253, 1089
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 2 cut(s) 370, 1127
CseI GACGC 2 cut(s) 118, 822
Csp6I GTAC 4 cut(s) 8, 57, 389, 821
CspAI ACCGGT 1 cut(s) 407
CviAII CATG 7 cut(s) 80, 190, 286, 425, 1040, 1078, 1131
CviQI GTAC 4 cut(s) 8, 57, 389, 821
DdeI CTNAG 5 cut(s) 245, 435, 802, 906, 1142
DpnI GATC 4 cut(s) 486, 510, 616, 735
DpnII GATC 4 cut(s) 484, 508, 614, 733
EaeI YGGCCR 2 cut(s) 282, 520
Eco130I CCWWGG 2 cut(s) 1039, 1130
Eco32I GATATC 1 cut(s) 856
Eco47I GGWCC 2 cut(s) 370, 1127
Eco72I CACGTG 1 cut(s) 764
EcoRII CCWGG 3 cut(s) 132, 496, 585
EcoRV GATATC 1 cut(s) 856
EcoT14I CCWWGG 2 cut(s) 1039, 1130
ErhI CCWWGG 2 cut(s) 1039, 1130
FaeI CATG 7 cut(s) 83, 193, 289, 428, 1043, 1081, 1134
FalI AAGNNNNNCTT 2 cut(s) 693, 725
FatI CATG 7 cut(s) 79, 189, 285, 424, 1039, 1077, 1130
FauNDI CATATG 1 cut(s) 396
Fnu4HI GCNGC 8 cut(s) 127, 168, 414, 567, 591, 603, 630, 663
FokI GGATG 4 cut(s) 25, 164, 692, 794
Fsp4HI GCNGC 8 cut(s) 127, 168, 414, 567, 591, 603, 630, 663
FspBI CTAG 1 cut(s) 788
FspI TGCGCA 1 cut(s) 1088
GlaI GCGC 2 cut(s) 252, 1088
GluI GCNGC 8 cut(s) 127, 168, 414, 567, 591, 603, 630, 663
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 2 cut(s) 284, 522
HapII CCGG 1 cut(s) 408
HgaI GACGC 2 cut(s) 118, 822
HhaI GCGC 2 cut(s) 253, 1089
Hin1II CATG 7 cut(s) 83, 193, 289, 428, 1043, 1081, 1134
Hin6I GCGC 2 cut(s) 251, 1087
HinP1I GCGC 2 cut(s) 251, 1087
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HinfI GANTC 3 cut(s) 26, 200, 904
HpaII CCGG 1 cut(s) 408
HphI GGTGA 2 cut(s) 565, 574
Hpy166II GTNNAC 2 cut(s) 25, 358
Hpy188I TCNGA 4 cut(s) 86, 531, 961, 1143
Hpy188III TCNNGA 2 cut(s) 837, 1078
Hpy8I GTNNAC 2 cut(s) 25, 358
HpyAV CCTTC 2 cut(s) 81, 724
HpyCH4III ACNGT 4 cut(s) 355, 459, 817, 1093
HpyCH4IV ACGT 1 cut(s) 763
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 257, 419, 599, 655, 701, 710, 974, 1014
HpyF3I CTNAG 5 cut(s) 245, 435, 802, 906, 1142
HpySE526I ACGT 1 cut(s) 763
Hsp92II CATG 7 cut(s) 83, 193, 289, 428, 1043, 1081, 1134
HspAI GCGC 2 cut(s) 251, 1087
Kzo9I GATC 4 cut(s) 484, 508, 614, 733
LmnI GCTCC 3 cut(s) 265, 953, 1134
Lsp1109I GCAGC 8 cut(s) 138, 154, 425, 553, 589, 602, 641, 674
LweI GCATC 3 cut(s) 510, 602, 965
MaeI CTAG 1 cut(s) 788
MaeII ACGT 1 cut(s) 763
MaeIII GTNAC 1 cut(s) 580
MalI GATC 4 cut(s) 486, 510, 616, 735
MboI GATC 4 cut(s) 484, 508, 614, 733
MboII GAAGA 2 cut(s) 263, 1066
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 508
MhlI GDGCHC 2 cut(s) 262, 801
MlsI TGGCCA 2 cut(s) 284, 522
MluCI AATT 6 cut(s) 159, 184, 205, 339, 516, 946
MluNI TGGCCA 2 cut(s) 284, 522
MlyI GAGTC 2 cut(s) 20, 898
MmeI TCCRAC 1 cut(s) 1078
MnlI CCTC 4 cut(s) 676, 720, 1101, 1137
Mox20I TGGCCA 2 cut(s) 284, 522
MscI TGGCCA 2 cut(s) 284, 522
MseI TTAA 7 cut(s) 218, 309, 380, 443, 470, 915, 1067
Msp20I TGGCCA 2 cut(s) 284, 522
MspI CCGG 1 cut(s) 408
MspR9I CCNGG 3 cut(s) 134, 498, 587
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 885
MvaI CCWGG 3 cut(s) 134, 498, 587
MvnI CGCG 1 cut(s) 833
MwoI GCNNNNNNNGC 9 cut(s) 110, 257, 419, 599, 655, 701, 710, 974, 1014
NcoI CCATGG 2 cut(s) 1039, 1130
NdeI CATATG 1 cut(s) 396
NdeII GATC 4 cut(s) 484, 508, 614, 733
NlaIII CATG 7 cut(s) 83, 193, 289, 428, 1043, 1081, 1134
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 580
NsbI TGCGCA 1 cut(s) 1088
NspI RCATGY 1 cut(s) 83
PagI TCATGA 1 cut(s) 1077
PctI GAATGC 1 cut(s) 885
PfeI GAWTC 1 cut(s) 200
PinAI ACCGGT 1 cut(s) 407
PkrI GCNGC 8 cut(s) 128, 169, 415, 568, 592, 604, 631, 664
PleI GAGTC 2 cut(s) 20, 898
PmaCI CACGTG 1 cut(s) 764
PmlI CACGTG 1 cut(s) 764
PpsI GAGTC 2 cut(s) 20, 898
Ppu21I YACGTR 1 cut(s) 764
PshBI ATTAAT 1 cut(s) 380
PsiI TTATAA 1 cut(s) 875
Psp6I CCWGG 3 cut(s) 132, 496, 585
PspCI CACGTG 1 cut(s) 764
PspGI CCWGG 3 cut(s) 132, 496, 585
PspN4I GGNNCC 1 cut(s) 372
PspPI GGNCC 2 cut(s) 370, 1127
PstI CTGCAG 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 529
PsuI RGATCY 1 cut(s) 508
RsaI GTAC 4 cut(s) 9, 58, 390, 822
RsaNI GTAC 4 cut(s) 8, 57, 389, 821
SaqAI TTAA 7 cut(s) 218, 309, 380, 443, 470, 915, 1067
SatI GCNGC 8 cut(s) 127, 168, 414, 567, 591, 603, 630, 663
Sau3AI GATC 4 cut(s) 484, 508, 614, 733
Sau96I GGNCC 2 cut(s) 370, 1127
ScaI AGTACT 1 cut(s) 9
SchI GAGTC 2 cut(s) 20, 898
ScrFI CCNGG 3 cut(s) 134, 498, 587
SduI GDGCHC 2 cut(s) 262, 801
SetI ASST 9 cut(s) 240, 568, 604, 691, 747, 760, 766, 873, 1139
SfaNI GCATC 3 cut(s) 510, 602, 965
SfcI CTRYAG 1 cut(s) 264
SinI GGWCC 2 cut(s) 370, 1127
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 6 cut(s) 159, 184, 205, 339, 516, 946
SsiI CCGC 1 cut(s) 677
SspI AATATT 1 cut(s) 378
SspMI CTAG 1 cut(s) 788
StyD4I CCNGG 3 cut(s) 132, 496, 585
StyI CCWWGG 2 cut(s) 1039, 1130
TaaI ACNGT 4 cut(s) 355, 459, 817, 1093
TaiI ACGT 1 cut(s) 766
TaqI TCGA 1 cut(s) 617
TasI AATT 6 cut(s) 159, 184, 205, 339, 516, 946
TatI WGTACW 4 cut(s) 7, 56, 388, 820
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 7 cut(s) 218, 309, 380, 443, 470, 915, 1067
Tru9I TTAA 7 cut(s) 218, 309, 380, 443, 470, 915, 1067
TscAI CASTG 2 cut(s) 1098, 1123
TseFI GTSAC 1 cut(s) 580
TseI GCWGC 8 cut(s) 126, 167, 413, 566, 590, 602, 629, 662
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 2 cut(s) 96, 1066
TspGWI ACGGA 3 cut(s) 87, 290, 879
TspRI CASTG 2 cut(s) 1098, 1123
VpaK11BI GGWCC 2 cut(s) 370, 1127
VspI ATTAAT 1 cut(s) 380
XceI RCATGY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 1046
XspI CTAG 1 cut(s) 788
ZrmI AGTACT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.