Rh3CG272400

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001107
Physical Location & Seq
Forward (+)
27025985 .. 27033233
7249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG272400.1

Sequence Viewer

Length: 1425 bp
ATGGCTATATATACTCTCCTAAATTGTTGCAAGAATATGGGCAAGATGCCCCTCCTTGTGTTCTTCAGACACACAAGGAATGTTTTCAAAGCTGATGAGCTAGGTGTGGAGATAGTACATATTGCACTTCCTACGCTACTAGCGTTAGTAGCAGACCCTGCTGCTTCTCTAATTGACACAGCATTTGTTGGCCATATAGGTCCAGTGGAGCTCGCAGCTGTGGGAATTTCTATTGCTGTTTTCAATCAAGTATCAAAAATAGCAATTTTCCCACTTGTCAGTATTACAACCTCTTTTGTTGCTGAAGAAGATACTACGGAACCATTAAGTACTAAAGAACAAGTATATGAAGAATTTGAAAATGCTACTTCTGTCAACAAGGAAATGGAAGTACTCAAACCACTTGTTGACTCTACACGATCATCCATTTCCAATGATAGTACAGAAATGGTCAAACTTGAGCATGTCAGAAGGCGTATTCCATCTGCTTCATCAGCATTGGTTATTGGCAGCGTCCTGGGGGTTATTCAAACTCTATTCCTTATTTTTGCTGCAAACCCCATCCTAAATTACATGGGGGTAGATTCTAATTCTCCTATGTTAAGACCAGCGAGACAATACCTGACACTAAGGTCACTTGGTGCTCCTGCAGTTCTTCTCTCTTTGGCCATGCAAGGGGTTTTCCGTGGTTTTAAGGATACAAAAACTCCTTTATATGCTACAATTGTGGGAGATACAGTAAACATCATTTTGGACCCAATATTAATGTTTGTACTCCATATGGGCATTACCGGTGCGGCCATTGCCCATGTTATCTCTCAGTATTTAATCTCCCTGATACTGTTATGGAGATTAAACAAACAAGTTGATCTCCTAACCCCAGGCATCAAAGATCTACGAATTGGCCAGTTTCTGAAAAATGGGGGTATGTTATTGGTGAGAGTTATAGCTGCAACATTCTGTGTCACCCTGGCAGCATCATTAGCTGCAAGGCAGGGATTGACATCAATGGCAGCATTTCAAGTTTGCTTGCAGATTTGGCTTGCAGCCTCTTTGCTTGCGGATGGGTTAGCTGTGGCTGGACAAGCAATCCTTGCAAGTGCATTTGCAAGGAAGGATCACAGCAAGGTCGTTGCCACAGCTTCACGTGTATTGCAGATGGCTTTGGTTCTAGGGATAGTGCTCTCAGTCATCCTTACAGTTGTACTAAAGTTTGCGTCAAGATTATTTACCGATGATATCGATGTTTTGCACCTTATAAGTTTAGGCATTCCGTTTATTGCAGTGACTCAGCCCATTAATGCTCTGGCATTTGTTTTTGATGGAGTAAATTATGGAGCATCAGATTTTGCATATTCTGCCTACTCAATGGCAAGTGTAGAAATCCATGTTGCTGTCTATTTTTCACTTGTATACTGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

474

Amino Acids

51.08

Weight (kDa)

6.75

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 163 - 254 1.5e-12 MatE
MatE PF01554 315 - 459 1.1e-12 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1259
AasI GACNNNNNNGTC 1 cut(s) 631
AccI GTMKAC 1 cut(s) 1413
AciI CCGC 2 cut(s) 797, 1061
AclWI GGATC 1 cut(s) 1125
AcoI YGGCCR 4 cut(s) 190, 666, 798, 904
AcsI RAATTY 2 cut(s) 225, 353
AcuI CTGAAG 2 cut(s) 49, 324
AcvI CACGTG 1 cut(s) 1148
AdeI CACNNNGTG 1 cut(s) 641
AfaI GTAC 6 cut(s) 117, 331, 393, 442, 774, 1206
AfiI CCNNNNNNNGG 1 cut(s) 675
AflIII ACRYGT 1 cut(s) 1147
AgeI ACCGGT 1 cut(s) 791
AgsI TTSAA 5 cut(s) 88, 244, 359, 530, 1022
AjnI CCWGG 3 cut(s) 516, 880, 969
AluBI AGCT 8 cut(s) 92, 100, 211, 218, 950, 986, 1073, 1142
AluI AGCT 8 cut(s) 92, 100, 211, 218, 950, 986, 1073, 1142
Alw21I GWGCWC 3 cut(s) 213, 646, 1185
Alw26I GTCTC 1 cut(s) 607
AlwI GGATC 1 cut(s) 1125
AlwNI CAGNNNCTG 2 cut(s) 158, 913
AoxI GGCC 4 cut(s) 190, 666, 798, 904
ApeKI GCWGC 9 cut(s) 161, 215, 510, 551, 950, 974, 986, 1013, 1046
ApoI RAATTY 2 cut(s) 225, 353
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AseI ATTAAT 2 cut(s) 764, 1299
AsiGI ACCGGT 1 cut(s) 791
Asp700I GAANNNNTTC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 200, 754
AsuHPI GGTGA 2 cut(s) 949, 958
AvaII GGWCC 2 cut(s) 200, 754
BalI TGGCCA 3 cut(s) 192, 668, 906
BanII GRGCYC 1 cut(s) 213
BbrPI CACGTG 1 cut(s) 1148
Bbv12I GWGCWC 3 cut(s) 213, 646, 1185
BbvI GCAGC 9 cut(s) 148, 227, 522, 538, 937, 973, 986, 1025, 1058
BccI CCATC 5 cut(s) 490, 569, 1058, 1153, 1316
BciT130I CCWGG 3 cut(s) 518, 882, 971
BciVI GTATCC 1 cut(s) 691
BcoDI GTCTC 1 cut(s) 607
BfaI CTAG 3 cut(s) 101, 140, 1172
BfmI CTRYAG 2 cut(s) 648, 1417
BfuI GTATCC 1 cut(s) 691
BglII AGATCT 1 cut(s) 892
BmcAI AGTACT 2 cut(s) 331, 393
Bme1390I CCNGG 3 cut(s) 518, 882, 971
Bme18I GGWCC 2 cut(s) 200, 754
BmgT120I GGNCC 2 cut(s) 200, 754
BmiI GGNNCC 2 cut(s) 321, 756
BmrFI CCNGG 3 cut(s) 518, 882, 971
BmsI GCATC 4 cut(s) 36, 894, 986, 1349
BpuEI CTTGAG 1 cut(s) 479
Bsa29I ATCGAT 1 cut(s) 1242
BsaAI YACGTR 1 cut(s) 1148
BsaBI GATNNNNATC 1 cut(s) 1003
BsaJI CCNNGG 4 cut(s) 517, 685, 880, 969
BsaWI WCCGGW 1 cut(s) 791
BsaXI ACNNNNNCTCC 4 cut(s) 691, 721, 723, 753
Bsc4I CCNNNNNNNGG 1 cut(s) 675
Bse118I RCCGGY 1 cut(s) 791
Bse1I ACTGG 2 cut(s) 203, 907
Bse3DI GCAATG 1 cut(s) 801
Bse8I GATNNNNATC 1 cut(s) 1003
BseBI CCWGG 3 cut(s) 518, 882, 971
BseCI ATCGAT 1 cut(s) 1242
BseDI CCNNGG 4 cut(s) 517, 685, 880, 969
BseGI GGATG 4 cut(s) 422, 561, 1069, 1191
BseJI GATNNNNATC 1 cut(s) 1003
BseLI CCNNNNNNNGG 1 cut(s) 675
BseMI GCAATG 1 cut(s) 801
BseMII CTCAG 3 cut(s) 833, 1200, 1304
BseNI ACTGG 2 cut(s) 203, 907
BseXI GCAGC 9 cut(s) 148, 227, 522, 538, 937, 973, 986, 1025, 1058
BshFI GGCC 4 cut(s) 192, 668, 800, 906
BshTI ACCGGT 1 cut(s) 791
BshVI ATCGAT 1 cut(s) 1242
BsiHKAI GWGCWC 3 cut(s) 213, 646, 1185
BsiSI CCGG 1 cut(s) 792
BslI CCNNNNNNNGG 1 cut(s) 675
BsmAI GTCTC 1 cut(s) 607
BsmI GAATGC 1 cut(s) 1269
BsnI GGCC 4 cut(s) 192, 668, 800, 906
Bsp1286I GDGCHC 3 cut(s) 213, 646, 1185
Bsp143I GATC 4 cut(s) 419, 868, 892, 1117
BspACI CCGC 2 cut(s) 797, 1061
BspANI GGCC 4 cut(s) 192, 668, 800, 906
BspCNI CTCAG 3 cut(s) 832, 1199, 1303
BspDI ATCGAT 1 cut(s) 1242
BspLI GGNNCC 2 cut(s) 321, 756
BspMAI CTGCAG 1 cut(s) 652
BspPI GGATC 1 cut(s) 1125
BsrDI GCAATG 1 cut(s) 801
BsrFI RCCGGY 1 cut(s) 791
BsrI ACTGG 2 cut(s) 203, 907
BssAI RCCGGY 1 cut(s) 791
BssECI CCNNGG 4 cut(s) 517, 685, 880, 969
BssMI GATC 4 cut(s) 419, 868, 892, 1117
BssNAI GTATAC 1 cut(s) 1414
Bst1107I GTATAC 1 cut(s) 1414
Bst2UI CCWGG 3 cut(s) 518, 882, 971
Bst4CI ACNGT 4 cut(s) 739, 843, 1201, 1418
BstAPI GCANNNNNTGC 3 cut(s) 158, 1094, 1358
BstBAI YACGTR 1 cut(s) 1148
BstC8I GCNNGC 4 cut(s) 213, 1031, 1044, 1059
BstDEI CTNAG 4 cut(s) 629, 819, 1186, 1290
BstDSI CCRYGG 1 cut(s) 685
BstF5I GGATG 4 cut(s) 422, 561, 1069, 1191
BstKTI GATC 4 cut(s) 422, 871, 895, 1120
BstMAI GTCTC 1 cut(s) 607
BstMBI GATC 4 cut(s) 419, 868, 892, 1117
BstMWI GCNNNNNNNGC 9 cut(s) 149, 158, 494, 803, 983, 1039, 1085, 1094, 1358
BstNI CCWGG 3 cut(s) 518, 882, 971
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 3 cut(s) 516, 880, 969
BstSFI CTRYAG 2 cut(s) 648, 1417
BstV1I GCAGC 9 cut(s) 148, 227, 522, 538, 937, 973, 986, 1025, 1058
BstX2I RGATCY 1 cut(s) 892
BstYI RGATCY 1 cut(s) 892
BstZ17I GTATAC 1 cut(s) 1414
Bsu15I ATCGAT 1 cut(s) 1242
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 4 cut(s) 192, 668, 800, 906
BsuTUI ATCGAT 1 cut(s) 1242
BtgI CCRYGG 1 cut(s) 685
BtsCI GGATG 4 cut(s) 422, 561, 1069, 1191
BtsI GCAGTG 1 cut(s) 1290
BtsIMutI CAGTG 2 cut(s) 210, 1290
Cac8I GCNNGC 4 cut(s) 213, 1031, 1044, 1059
CaiI CAGNNNCTG 2 cut(s) 158, 913
Cfr10I RCCGGY 1 cut(s) 791
Cfr13I GGNCC 2 cut(s) 200, 754
ClaI ATCGAT 1 cut(s) 1242
CseI GACGC 2 cut(s) 502, 1206
Csp6I GTAC 6 cut(s) 116, 330, 392, 441, 773, 1205
CspAI ACCGGT 1 cut(s) 791
CviAII CATG 5 cut(s) 464, 574, 670, 809, 1388
CviQI GTAC 6 cut(s) 116, 330, 392, 441, 773, 1205
DdeI CTNAG 4 cut(s) 629, 819, 1186, 1290
DpnI GATC 4 cut(s) 421, 870, 894, 1119
DpnII GATC 4 cut(s) 419, 868, 892, 1117
DraIII CACNNNGTG 1 cut(s) 641
DrdI GACNNNNNNGTC 1 cut(s) 631
DseDI GACNNNNNNGTC 1 cut(s) 631
EaeI YGGCCR 4 cut(s) 190, 666, 798, 904
Ecl136II GAGCTC 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 1240
Eco47I GGWCC 2 cut(s) 200, 754
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 49, 324
Eco72I CACGTG 1 cut(s) 1148
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 3 cut(s) 516, 880, 969
EcoRV GATATC 1 cut(s) 1240
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 5 cut(s) 467, 577, 673, 812, 1391
FalI AAGNNNNNCTT 2 cut(s) 1077, 1109
FatI CATG 5 cut(s) 463, 573, 669, 808, 1387
FauNDI CATATG 1 cut(s) 780
FblI GTMKAC 1 cut(s) 1413
FokI GGATG 4 cut(s) 409, 548, 1076, 1178
FriOI GRGCYC 1 cut(s) 213
FspBI CTAG 3 cut(s) 101, 140, 1172
HaeIII GGCC 4 cut(s) 192, 668, 800, 906
HapII CCGG 1 cut(s) 792
HgaI GACGC 2 cut(s) 502, 1206
Hin1II CATG 5 cut(s) 467, 577, 673, 812, 1391
HincII GTYRAC 2 cut(s) 376, 409
HindII GTYRAC 2 cut(s) 376, 409
HinfI GANTC 3 cut(s) 410, 584, 1288
HpaII CCGG 1 cut(s) 792
HphI GGTGA 2 cut(s) 949, 958
Hpy166II GTNNAC 4 cut(s) 376, 409, 742, 1414
Hpy188I TCNGA 4 cut(s) 68, 470, 915, 1345
Hpy188III TCNNGA 1 cut(s) 1221
Hpy8I GTNNAC 4 cut(s) 376, 409, 742, 1414
HpyAV CCTTC 2 cut(s) 465, 1108
HpyCH4III ACNGT 4 cut(s) 739, 843, 1201, 1418
HpyCH4IV ACGT 1 cut(s) 1147
HpyF10VI GCNNNNNNNGC 9 cut(s) 149, 158, 494, 803, 983, 1039, 1085, 1094, 1358
HpyF3I CTNAG 4 cut(s) 629, 819, 1186, 1290
HpySE526I ACGT 1 cut(s) 1147
Hsp92II CATG 5 cut(s) 467, 577, 673, 812, 1391
Kzo9I GATC 4 cut(s) 419, 868, 892, 1117
LmnI GCTCC 3 cut(s) 208, 649, 1337
Lsp1109I GCAGC 9 cut(s) 148, 227, 522, 538, 937, 973, 986, 1025, 1058
LweI GCATC 4 cut(s) 36, 894, 986, 1349
MaeI CTAG 3 cut(s) 101, 140, 1172
MaeII ACGT 1 cut(s) 1147
MaeIII GTNAC 3 cut(s) 633, 964, 1285
MalI GATC 4 cut(s) 421, 870, 894, 1119
MboI GATC 4 cut(s) 419, 868, 892, 1117
MboII GAAGA 5 cut(s) 55, 317, 320, 362, 647
MfeI CAATTG 1 cut(s) 723
MflI RGATCY 1 cut(s) 892
MhlI GDGCHC 3 cut(s) 213, 646, 1185
MlsI TGGCCA 3 cut(s) 192, 668, 906
MluNI TGGCCA 3 cut(s) 192, 668, 906
MlyI GAGTC 2 cut(s) 404, 1282
MnlI CCTC 3 cut(s) 62, 301, 1060
Mox20I TGGCCA 3 cut(s) 192, 668, 906
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 3 cut(s) 192, 668, 906
MseI TTAA 7 cut(s) 326, 602, 693, 764, 827, 854, 1299
Msp20I TGGCCA 3 cut(s) 192, 668, 906
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 1 cut(s) 792
MspR9I CCNGG 3 cut(s) 518, 882, 971
MunI CAATTG 1 cut(s) 723
Mva1269I GAATGC 1 cut(s) 1269
MvaI CCWGG 3 cut(s) 518, 882, 971
MwoI GCNNNNNNNGC 9 cut(s) 149, 158, 494, 803, 983, 1039, 1085, 1094, 1358
NdeI CATATG 1 cut(s) 780
NdeII GATC 4 cut(s) 419, 868, 892, 1117
NlaIII CATG 5 cut(s) 467, 577, 673, 812, 1391
NlaIV GGNNCC 2 cut(s) 321, 756
NmuCI GTSAC 3 cut(s) 633, 964, 1285
NspI RCATGY 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PctI GAATGC 1 cut(s) 1269
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 584
PinAI ACCGGT 1 cut(s) 791
PleI GAGTC 2 cut(s) 404, 1282
PmaCI CACGTG 1 cut(s) 1148
PmlI CACGTG 1 cut(s) 1148
PpsI GAGTC 2 cut(s) 404, 1282
Ppu21I YACGTR 1 cut(s) 1148
PshBI ATTAAT 2 cut(s) 764, 1299
PsiI TTATAA 1 cut(s) 1259
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 3 cut(s) 516, 880, 969
PspCI CACGTG 1 cut(s) 1148
PspGI CCWGG 3 cut(s) 516, 880, 969
PspN4I GGNNCC 2 cut(s) 321, 756
PspPI GGNCC 2 cut(s) 200, 754
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 2 cut(s) 158, 913
PsuI RGATCY 1 cut(s) 892
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 6 cut(s) 117, 331, 393, 442, 774, 1206
RsaNI GTAC 6 cut(s) 116, 330, 392, 441, 773, 1205
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 7 cut(s) 326, 602, 693, 764, 827, 854, 1299
Sau3AI GATC 4 cut(s) 419, 868, 892, 1117
Sau96I GGNCC 2 cut(s) 200, 754
ScaI AGTACT 2 cut(s) 331, 393
SchI GAGTC 2 cut(s) 404, 1282
ScrFI CCNGG 3 cut(s) 518, 882, 971
SduI GDGCHC 3 cut(s) 213, 646, 1185
SfaNI GCATC 4 cut(s) 36, 894, 986, 1349
SfcI CTRYAG 2 cut(s) 648, 1417
SinI GGWCC 2 cut(s) 200, 754
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SsiI CCGC 2 cut(s) 797, 1061
SspI AATATT 1 cut(s) 762
SspMI CTAG 3 cut(s) 101, 140, 1172
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 3 cut(s) 516, 880, 969
TaaI ACNGT 4 cut(s) 739, 843, 1201, 1418
TaiI ACGT 1 cut(s) 1150
TaqI TCGA 1 cut(s) 1242
TatI WGTACW 6 cut(s) 115, 329, 391, 440, 772, 1204
TauI GCSGC 1 cut(s) 800
TfiI GAWTC 1 cut(s) 584
Tru1I TTAA 7 cut(s) 326, 602, 693, 764, 827, 854, 1299
Tru9I TTAA 7 cut(s) 326, 602, 693, 764, 827, 854, 1299
TscAI CASTG 2 cut(s) 210, 1290
TseFI GTSAC 3 cut(s) 633, 964, 1285
TseI GCWGC 9 cut(s) 161, 215, 510, 551, 950, 974, 986, 1013, 1046
Tsp45I GTSAC 3 cut(s) 633, 964, 1285
TspDTI ATGAA 2 cut(s) 363, 480
TspGWI ACGGA 3 cut(s) 332, 674, 1263
TspRI CASTG 2 cut(s) 210, 1290
VpaK11BI GGWCC 2 cut(s) 200, 754
VspI ATTAAT 2 cut(s) 764, 1299
XapI RAATTY 2 cut(s) 225, 353
XceI RCATGY 1 cut(s) 467
XcmI CCANNNNNNNNNTGG 1 cut(s) 1303
XmiI GTMKAC 1 cut(s) 1413
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 3 cut(s) 101, 140, 1172
ZrmI AGTACT 2 cut(s) 331, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.