Rw3G021650

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001107
Physical Location & Seq
Forward (+)
26248718 .. 26255844
7127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G021650.1

Sequence Viewer

Length: 1194 bp
ATGGACGTACTCAAACCACTTGTTGACTCTACACCATCATCCATTTCCAATGATAGTACAGAAATGGTCAAACTTGAGCATGTCAGAAGGCGTATTCCATCTGCTTCATCAGCATTGGTTATTGGCAGCGTCCTGGGGGTTGTTCAAACTCTATTCCTTATTTTTGCTGCAAACCCCATCCTAAATTACATGGGGGTAGATTCTAATTCTCCTATGTTAAGACCAGCGAGACAATACCTGACACTAAGGTCACTTGGTGCTCCTGCAGTTCTTCTCTCTTTGGCCATGCAGGGGGTTTTCCGTGGTTTTAAGGATACAAAAACTCCTTATATGCTACAACCATTCATATTTGCTTCTTTAACTGCAGTTGTGGGAGATACAGTAAACATCATTTTGGACCCAATATTAATGTTTGTACTCCATATGGGCATTACCGGTGCGGCCATTGCCCATGTTATCTCTCAGTATTTAATCTCCCTGATACTGTTATGGAGATTAAACAAACAAGTTGATCTCCTAACCCCAGGCATCAAAGATCTACGAATTGGCCAGTTTCTGAAAAATGGGGGTATGTTATTGGTGAGAGTTATAGCTGCAACATTCTGTGTCACCCTGGCAGCATCATTAGCTGCGAGGCAGGGATCGACATCAATGGCAGCATTTCAAGTTTGCTTGCAGATTTGGCTTGCAGCCTCTTTGCTTGCGGATGGGTTAGCTGTGGCTGGACAAGCAATCCTTGCAAGTGCATTTGCAAGGAAGGATCACAGCAAGGTCGTTGCCAACAGCTTCACGTGTATTGCAGATGCTTTGGTTCTAGGGATAGTGCTCTCAGTCATCCTTACAGTTGTACTAAAGTTTGCGTCAAGATTATTTACCGATGATATCGATGTTTTGCACCTTATAAGTTTAGGCATTCCGTTTATTGCAGTGACTCAGCCCATTAATGCTCTGGCATTTGTTTTTGATGGAGTAAATTATGGGCATCAGATTTTGCATATCTGCCTACTCAATGGCAACATTCTCATATTCTCTGTTGAATCCAGGTGTTGTGGCTTGTGCCTGTTTCTATTATCATCGAGCCATGGCTTTGTTGGTATCTGGGTTGCTTTAACAATCTTCATGAGTTTGCGCACAGTGGTTGGATTTTGGAGGATAGGGACTGGAACTGGACCATGGAGCTTTCTGAGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

397

Amino Acids

42.8

Weight (kDa)

9.15

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 35 - 113 3.9e-08 MatE
MatE PF01554 195 - 331 1.3e-11 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 902
AasI GACNNNNNNGTC 1 cut(s) 247
Acc16I TGCGCA 1 cut(s) 1130
AciI CCGC 2 cut(s) 440, 704
AclWI GGATC 2 cut(s) 649, 768
AcoI YGGCCR 3 cut(s) 282, 441, 547
AcvI CACGTG 1 cut(s) 792
AdeI CACNNNGTG 1 cut(s) 257
AfaI GTAC 4 cut(s) 9, 58, 417, 849
AfiI CCNNNNNNNGG 1 cut(s) 291
AflIII ACRYGT 1 cut(s) 791
AgeI ACCGGT 1 cut(s) 434
AgsI TTSAA 3 cut(s) 146, 665, 1037
AjnI CCWGG 4 cut(s) 132, 523, 612, 1040
AluBI AGCT 5 cut(s) 593, 629, 716, 786, 1179
AluI AGCT 5 cut(s) 593, 629, 716, 786, 1179
Alw21I GWGCWC 2 cut(s) 262, 828
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 2 cut(s) 649, 768
AlwNI CAGNNNCTG 1 cut(s) 556
AoxI GGCC 3 cut(s) 282, 441, 547
ApeKI GCWGC 7 cut(s) 126, 167, 593, 617, 629, 656, 689
AseI ATTAAT 2 cut(s) 407, 942
AsiGI ACCGGT 1 cut(s) 434
AspLEI GCGC 1 cut(s) 1131
AspS9I GGNCC 2 cut(s) 397, 1169
AsuHPI GGTGA 2 cut(s) 592, 601
AvaII GGWCC 2 cut(s) 397, 1169
BalI TGGCCA 2 cut(s) 284, 549
BbrPI CACGTG 1 cut(s) 792
Bbv12I GWGCWC 2 cut(s) 262, 828
BbvI GCAGC 7 cut(s) 138, 154, 580, 616, 629, 668, 701
BccI CCATC 5 cut(s) 43, 106, 185, 701, 959
BciT130I CCWGG 4 cut(s) 134, 525, 614, 1042
BciVI GTATCC 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 1 cut(s) 815
BfmI CTRYAG 2 cut(s) 264, 363
BfuI GTATCC 1 cut(s) 307
BglII AGATCT 1 cut(s) 535
BisI GCNGC 8 cut(s) 127, 168, 441, 594, 618, 630, 657, 690
BlsI GCNGC 8 cut(s) 128, 169, 442, 595, 619, 631, 658, 691
Bme1390I CCNGG 4 cut(s) 134, 525, 614, 1042
Bme18I GGWCC 2 cut(s) 397, 1169
BmgT120I GGNCC 2 cut(s) 397, 1169
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 4 cut(s) 134, 525, 614, 1042
BmsI GCATC 4 cut(s) 537, 629, 793, 991
BpuEI CTTGAG 1 cut(s) 95
Bsa29I ATCGAT 1 cut(s) 885
BsaAI YACGTR 1 cut(s) 792
BsaBI GATNNNNATC 1 cut(s) 646
BsaJI CCNNGG 6 cut(s) 133, 301, 523, 612, 1081, 1172
BsaWI WCCGGW 1 cut(s) 434
BsaXI ACNNNNNCTCC 4 cut(s) 307, 337, 366, 396
Bsc4I CCNNNNNNNGG 1 cut(s) 291
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 3 cut(s) 550, 1165, 1171
Bse3DI GCAATG 1 cut(s) 444
Bse8I GATNNNNATC 1 cut(s) 646
BseBI CCWGG 4 cut(s) 134, 525, 614, 1042
BseCI ATCGAT 1 cut(s) 885
BseDI CCNNGG 6 cut(s) 133, 301, 523, 612, 1081, 1172
BseGI GGATG 4 cut(s) 38, 177, 712, 834
BseJI GATNNNNATC 1 cut(s) 646
BseLI CCNNNNNNNGG 1 cut(s) 291
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 4 cut(s) 476, 843, 947, 1175
BseNI ACTGG 3 cut(s) 550, 1165, 1171
BseXI GCAGC 7 cut(s) 138, 154, 580, 616, 629, 668, 701
BshFI GGCC 3 cut(s) 284, 443, 549
BshTI ACCGGT 1 cut(s) 434
BshVI ATCGAT 1 cut(s) 885
BsiHKAI GWGCWC 2 cut(s) 262, 828
BsiSI CCGG 1 cut(s) 435
BslFI GGGAC 1 cut(s) 1171
BslI CCNNNNNNNGG 1 cut(s) 291
BsmAI GTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 1171
BsmI GAATGC 1 cut(s) 912
BsnI GGCC 3 cut(s) 284, 443, 549
Bsp1286I GDGCHC 2 cut(s) 262, 828
Bsp143I GATC 4 cut(s) 511, 535, 641, 760
Bsp19I CCATGG 2 cut(s) 1081, 1172
BspACI CCGC 2 cut(s) 440, 704
BspANI GGCC 3 cut(s) 284, 443, 549
BspCNI CTCAG 4 cut(s) 475, 842, 946, 1176
BspDI ATCGAT 1 cut(s) 885
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 1 cut(s) 399
BspMAI CTGCAG 2 cut(s) 268, 367
BspPI GGATC 2 cut(s) 649, 768
BsrDI GCAATG 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 434
BsrI ACTGG 3 cut(s) 550, 1165, 1171
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 6 cut(s) 133, 301, 523, 612, 1081, 1172
BssMI GATC 4 cut(s) 511, 535, 641, 760
BssT1I CCWWGG 2 cut(s) 1081, 1172
Bst2UI CCWGG 4 cut(s) 134, 525, 614, 1042
Bst4CI ACNGT 4 cut(s) 382, 486, 844, 1135
BstAPI GCANNNNNTGC 1 cut(s) 737
BstBAI YACGTR 1 cut(s) 792
BstC8I GCNNGC 3 cut(s) 674, 687, 702
BstDEI CTNAG 5 cut(s) 245, 462, 829, 933, 1184
BstDSI CCRYGG 3 cut(s) 301, 1081, 1172
BstF5I GGATG 4 cut(s) 38, 177, 712, 834
BstHHI GCGC 1 cut(s) 1131
BstKTI GATC 4 cut(s) 514, 538, 644, 763
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 4 cut(s) 511, 535, 641, 760
BstMWI GCNNNNNNNGC 6 cut(s) 110, 446, 626, 682, 728, 737
BstNI CCWGG 4 cut(s) 134, 525, 614, 1042
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 4 cut(s) 132, 523, 612, 1040
BstSFI CTRYAG 2 cut(s) 264, 363
BstV1I GCAGC 7 cut(s) 138, 154, 580, 616, 629, 668, 701
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
Bsu15I ATCGAT 1 cut(s) 885
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 3 cut(s) 284, 443, 549
BsuTUI ATCGAT 1 cut(s) 885
BtgI CCRYGG 3 cut(s) 301, 1081, 1172
BtsCI GGATG 4 cut(s) 38, 177, 712, 834
BtsI GCAGTG 1 cut(s) 933
BtsIMutI CAGTG 2 cut(s) 933, 1140
Cac8I GCNNGC 3 cut(s) 674, 687, 702
CaiI CAGNNNCTG 1 cut(s) 556
CciI TCATGA 1 cut(s) 1119
CfoI GCGC 1 cut(s) 1131
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 397, 1169
ClaI ATCGAT 1 cut(s) 885
CseI GACGC 2 cut(s) 118, 849
Csp6I GTAC 4 cut(s) 8, 57, 416, 848
CspAI ACCGGT 1 cut(s) 434
CviAII CATG 7 cut(s) 80, 190, 286, 452, 1082, 1120, 1173
CviQI GTAC 4 cut(s) 8, 57, 416, 848
DdeI CTNAG 5 cut(s) 245, 462, 829, 933, 1184
DpnI GATC 4 cut(s) 513, 537, 643, 762
DpnII GATC 4 cut(s) 511, 535, 641, 760
DraIII CACNNNGTG 1 cut(s) 257
DrdI GACNNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 247
EaeI YGGCCR 3 cut(s) 282, 441, 547
Eco130I CCWWGG 2 cut(s) 1081, 1172
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 2 cut(s) 397, 1169
Eco72I CACGTG 1 cut(s) 792
EcoRII CCWGG 4 cut(s) 132, 523, 612, 1040
EcoRV GATATC 1 cut(s) 883
EcoT14I CCWWGG 2 cut(s) 1081, 1172
ErhI CCWWGG 2 cut(s) 1081, 1172
FaeI CATG 7 cut(s) 83, 193, 289, 455, 1085, 1123, 1176
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 1 cut(s) 1171
FatI CATG 7 cut(s) 79, 189, 285, 451, 1081, 1119, 1172
FauNDI CATATG 1 cut(s) 423
Fnu4HI GCNGC 8 cut(s) 127, 168, 441, 594, 618, 630, 657, 690
FokI GGATG 4 cut(s) 25, 164, 719, 821
Fsp4HI GCNGC 8 cut(s) 127, 168, 441, 594, 618, 630, 657, 690
FspBI CTAG 1 cut(s) 815
FspI TGCGCA 1 cut(s) 1130
GlaI GCGC 1 cut(s) 1130
GluI GCNGC 8 cut(s) 127, 168, 441, 594, 618, 630, 657, 690
HaeIII GGCC 3 cut(s) 284, 443, 549
HapII CCGG 1 cut(s) 435
HgaI GACGC 2 cut(s) 118, 849
HhaI GCGC 1 cut(s) 1131
Hin1II CATG 7 cut(s) 83, 193, 289, 455, 1085, 1123, 1176
Hin6I GCGC 1 cut(s) 1129
HinP1I GCGC 1 cut(s) 1129
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HinfI GANTC 4 cut(s) 26, 200, 931, 1037
HpaII CCGG 1 cut(s) 435
HphI GGTGA 2 cut(s) 592, 601
Hpy166II GTNNAC 2 cut(s) 25, 385
Hpy188I TCNGA 4 cut(s) 86, 558, 987, 1185
Hpy188III TCNNGA 2 cut(s) 864, 1120
Hpy8I GTNNAC 2 cut(s) 25, 385
HpyAV CCTTC 2 cut(s) 81, 751
HpyCH4III ACNGT 4 cut(s) 382, 486, 844, 1135
HpyCH4IV ACGT 2 cut(s) 6, 791
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 446, 626, 682, 728, 737
HpyF3I CTNAG 5 cut(s) 245, 462, 829, 933, 1184
HpySE526I ACGT 2 cut(s) 6, 791
Hsp92II CATG 7 cut(s) 83, 193, 289, 455, 1085, 1123, 1176
HspAI GCGC 1 cut(s) 1129
Kzo9I GATC 4 cut(s) 511, 535, 641, 760
LmnI GCTCC 2 cut(s) 265, 1176
Lsp1109I GCAGC 7 cut(s) 138, 154, 580, 616, 629, 668, 701
LweI GCATC 4 cut(s) 537, 629, 793, 991
MaeI CTAG 1 cut(s) 815
MaeII ACGT 2 cut(s) 6, 791
MaeIII GTNAC 3 cut(s) 249, 607, 928
MalI GATC 4 cut(s) 513, 537, 643, 762
MboI GATC 4 cut(s) 511, 535, 641, 760
MboII GAAGA 2 cut(s) 263, 1108
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 2 cut(s) 262, 828
MlsI TGGCCA 2 cut(s) 284, 549
MluCI AATT 4 cut(s) 184, 205, 543, 973
MluNI TGGCCA 2 cut(s) 284, 549
MlyI GAGTC 2 cut(s) 20, 925
MmeI TCCRAC 1 cut(s) 1120
MnlI CCTC 4 cut(s) 627, 703, 1143, 1179
Mox20I TGGCCA 2 cut(s) 284, 549
MscI TGGCCA 2 cut(s) 284, 549
MseI TTAA 8 cut(s) 218, 309, 359, 407, 470, 497, 942, 1109
Msp20I TGGCCA 2 cut(s) 284, 549
MspI CCGG 1 cut(s) 435
MspR9I CCNGG 4 cut(s) 134, 525, 614, 1042
Mva1269I GAATGC 1 cut(s) 912
MvaI CCWGG 4 cut(s) 134, 525, 614, 1042
MwoI GCNNNNNNNGC 6 cut(s) 110, 446, 626, 682, 728, 737
NcoI CCATGG 2 cut(s) 1081, 1172
NdeI CATATG 1 cut(s) 423
NdeII GATC 4 cut(s) 511, 535, 641, 760
NlaIII CATG 7 cut(s) 83, 193, 289, 455, 1085, 1123, 1176
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 3 cut(s) 249, 607, 928
NsbI TGCGCA 1 cut(s) 1130
NspI RCATGY 1 cut(s) 83
PagI TCATGA 1 cut(s) 1119
PctI GAATGC 1 cut(s) 912
PfeI GAWTC 2 cut(s) 200, 1037
PinAI ACCGGT 1 cut(s) 434
PkrI GCNGC 8 cut(s) 128, 169, 442, 595, 619, 631, 658, 691
PleI GAGTC 2 cut(s) 20, 925
PmaCI CACGTG 1 cut(s) 792
PmlI CACGTG 1 cut(s) 792
PpsI GAGTC 2 cut(s) 20, 925
Ppu21I YACGTR 1 cut(s) 792
PshBI ATTAAT 2 cut(s) 407, 942
PsiI TTATAA 1 cut(s) 902
Psp6I CCWGG 4 cut(s) 132, 523, 612, 1040
PspCI CACGTG 1 cut(s) 792
PspGI CCWGG 4 cut(s) 132, 523, 612, 1040
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 2 cut(s) 397, 1169
PstI CTGCAG 2 cut(s) 268, 367
PstNI CAGNNNCTG 1 cut(s) 556
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 4 cut(s) 9, 58, 417, 849
RsaNI GTAC 4 cut(s) 8, 57, 416, 848
SaqAI TTAA 8 cut(s) 218, 309, 359, 407, 470, 497, 942, 1109
SatI GCNGC 8 cut(s) 127, 168, 441, 594, 618, 630, 657, 690
Sau3AI GATC 4 cut(s) 511, 535, 641, 760
Sau96I GGNCC 2 cut(s) 397, 1169
SchI GAGTC 2 cut(s) 20, 925
ScrFI CCNGG 4 cut(s) 134, 525, 614, 1042
SduI GDGCHC 2 cut(s) 262, 828
SfaNI GCATC 4 cut(s) 537, 629, 793, 991
SfcI CTRYAG 2 cut(s) 264, 363
SinI GGWCC 2 cut(s) 397, 1169
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 4 cut(s) 184, 205, 543, 973
SsiI CCGC 2 cut(s) 440, 704
SspI AATATT 1 cut(s) 405
SspMI CTAG 1 cut(s) 815
StyD4I CCNGG 4 cut(s) 132, 523, 612, 1040
StyI CCWWGG 2 cut(s) 1081, 1172
TaaI ACNGT 4 cut(s) 382, 486, 844, 1135
TaiI ACGT 2 cut(s) 9, 794
TaqI TCGA 3 cut(s) 644, 885, 1076
TasI AATT 4 cut(s) 184, 205, 543, 973
TatI WGTACW 3 cut(s) 56, 415, 847
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 2 cut(s) 200, 1037
Tru1I TTAA 8 cut(s) 218, 309, 359, 407, 470, 497, 942, 1109
Tru9I TTAA 8 cut(s) 218, 309, 359, 407, 470, 497, 942, 1109
TscAI CASTG 2 cut(s) 933, 1140
TseFI GTSAC 3 cut(s) 249, 607, 928
TseI GCWGC 7 cut(s) 126, 167, 593, 617, 629, 656, 689
Tsp45I GTSAC 3 cut(s) 249, 607, 928
TspDTI ATGAA 3 cut(s) 96, 334, 1108
TspGWI ACGGA 2 cut(s) 290, 906
TspRI CASTG 2 cut(s) 933, 1140
VpaK11BI GGWCC 2 cut(s) 397, 1169
VspI ATTAAT 2 cut(s) 407, 942
XceI RCATGY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 2 cut(s) 946, 1088
XspI CTAG 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.