Rroxscaffold_6G00400490

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001107
Physical Location & Seq
Reverse (-)
22586682 .. 22595288
8607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400490.1

Sequence Viewer

Length: 1701 bp
ATGATACGCAACAAACCTCTCACCGCAAAAACCTCTCATCTTCGCAACTCATGTGTTTCTAGCTCCCACGCCACATACCTTCTTGCAAGCCTTTTATGCACTTTTGTTCATCTATCAAAGCTATATGAAGCTTCTACCTCACCATCACCGAAGCCCGTCACCGCGGCGGCCAACCTTACGTGGGGAATAAGGAAAGACGCTACAAAGCTTCTTGAAGCTTTGGACCCGCTTCTGAACTCAAAAGGGCTCCGTGAGGAAGGCCTTATTCTAATCATGGCTATATATACTCTCCTAAATTGTTGCAAGAATATGGGCAAGATGCCCCTCCTTGTGTTCTTGAGACACACAAGGAATGTTTTTAAAGTTGATGAGCTAGGTGTGGAGATAGTACATATTGCACTTCCTACGCTACTAGCATTAGTAGCAGACCCTGCTGCTTCTCTAATTGACACAGCATTTGTTGGCCATATAGGTCCAGTGGAGCTCGCAGCAGTGGGAATTTCTATTGCTGTTTTCAATCAAGTATCAAAAATAGCAATTTTTCCACTTGTCAGTATTACAACCTCTTTTGTTGCTGAAGAAGATACTACAGAACCATTAAGTACTAAAGAACAAGTATATGAAGAATTTGAAAATGCTACTTCCGTCAACAAGGAAATGGAATGTCCTGGGGTTATTCAAGCTCTATTCCTAATTTTTGCTGCAAACCCCATCCTAAATTACATGGGGGTAGATTCTAATTCTCCTATGTTACGACCAGCGAGACAATACCTGACACTAAGGTCACTTGGTGCTCCTGCAGTTCTTCTCTCTTTAGCCATGCAAGGAGTTTTCCGTGGTTTTAAGGATACAAAAACTCCTTTATATGCTACAATTGTGGGAGATACAGTAAACATCATTTTGGACCCAATATTAATGTTTGTACTCCATATGGGCATTACCGGTGCGGCCATTGCCCATGTTATCTCTCAGTATTTAATCTCCCTGATACTGTTATGGAGATTAAACAAACAAGTTGATCTCCTAACCCCAGGCATCAAAGATCTACGAATTGGCCAGTTTCTGAAAAATGGGGGTATGTTATTGGTGAGAGTTATAGCTGCAACATTCTGTGTCACCCTGGCAGCATCATTAGCTGCAAGGCAGGGACCGACATCAATGGCAGCATTTCAAGTTTGCTTGCAGATTTGGCTTGCAGCCTCTTTGCTTGCAGATGGCTTAGCTGTGGCTGGACAAATGGCTTTGGTTCTAGGGATAGTGCTTTCAGTCATCCTTACAGTTGTACTAAAGTTCGCGTCAAGATTATTTACTGATGATATCAATGTTTTGCACCTTATAAGTTTAGGCATTCCGTTTATTGCAGTGACTCAGCCCGTTAATGCTCTGGCATTTGTTTTTGATGGAGTAAATTATGGAGCATCAGATTTTGCGTATTCTGCCTACTCAATGGCAAGTGTAGAAATCCATGTTGCTGTCTATTTTTCACTTGTGTTGGTGGCTATAGTGAGCATATTGTGCCTGTTTCTATTATCATCAAGCCATGGCTTTGTTGGTATCTGGGTTGCTTTAACAATCTTCATGAGTTTGCGCACAGTGGTTGGATTTTGGAGAAGTTGCTTCCACATTGATCTTGAACTTGCATTACAGGATAGGGACTGGAACTGGACCATGGAGCTTTCTGAGGGAGTAATTGAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

566

Amino Acids

61.41

Weight (kDa)

6.31

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 225 - 304 1.8e-10 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1337
AasI GACNNNNNNGTC 1 cut(s) 781
Acc16I TGCGCA 1 cut(s) 1589
AccII CGCG 2 cut(s) 164, 1295
AciI CCGC 6 cut(s) 24, 162, 164, 167, 227, 947
AcoI YGGCCR 4 cut(s) 168, 463, 948, 1054
AcsI RAATTY 2 cut(s) 498, 626
AcuI CTGAAG 1 cut(s) 597
AdeI CACNNNGTG 1 cut(s) 791
AfaI GTAC 4 cut(s) 390, 604, 924, 1284
AfiI CCNNNNNNNGG 1 cut(s) 181
AgeI ACCGGT 1 cut(s) 941
AgsI TTSAA 7 cut(s) 215, 517, 632, 680, 1172, 1634, 1694
AjnI CCWGG 3 cut(s) 667, 1030, 1119
Alw21I GWGCWC 2 cut(s) 486, 796
Alw26I GTCTC 2 cut(s) 334, 757
AlwNI CAGNNNCTG 2 cut(s) 431, 1063
AoxI GGCC 5 cut(s) 168, 259, 463, 948, 1054
ApeKI GCWGC 8 cut(s) 434, 488, 701, 1100, 1124, 1136, 1163, 1196
ApoI RAATTY 2 cut(s) 498, 626
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AseI ATTAAT 1 cut(s) 914
AsiGI ACCGGT 1 cut(s) 941
AspLEI GCGC 1 cut(s) 1590
AspS9I GGNCC 5 cut(s) 223, 473, 904, 1148, 1665
AsuHPI GGTGA 6 cut(s) 13, 132, 138, 151, 1099, 1108
AvaII GGWCC 5 cut(s) 223, 473, 904, 1148, 1665
BalI TGGCCA 2 cut(s) 465, 1056
BanII GRGCYC 2 cut(s) 249, 486
Bbv12I GWGCWC 2 cut(s) 486, 796
BbvI GCAGC 8 cut(s) 421, 500, 688, 1087, 1123, 1136, 1175, 1208
BccI CCATC 4 cut(s) 151, 719, 1208, 1394
BciT130I CCWGG 3 cut(s) 669, 1032, 1121
BciVI GTATCC 1 cut(s) 841
BcoDI GTCTC 2 cut(s) 334, 757
BfaI CTAG 4 cut(s) 60, 374, 413, 1250
BfmI CTRYAG 3 cut(s) 588, 798, 1500
BfuI GTATCC 1 cut(s) 841
BglII AGATCT 1 cut(s) 1042
BlpI GCTNAGC 1 cut(s) 1219
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 3 cut(s) 669, 1032, 1121
Bme18I GGWCC 5 cut(s) 223, 473, 904, 1148, 1665
BmgT120I GGNCC 5 cut(s) 223, 473, 904, 1148, 1665
BmiI GGNNCC 4 cut(s) 225, 248, 906, 1149
BmrFI CCNGG 3 cut(s) 669, 1032, 1121
BmsI GCATC 4 cut(s) 309, 1044, 1136, 1427
Bpu1102I GCTNAGC 1 cut(s) 1219
BpuEI CTTGAG 1 cut(s) 358
BsaAI YACGTR 1 cut(s) 180
BsaJI CCNNGG 7 cut(s) 162, 668, 835, 1030, 1119, 1540, 1668
BsaWI WCCGGW 1 cut(s) 941
BsaXI ACNNNNNCTCC 4 cut(s) 841, 871, 873, 903
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse118I RCCGGY 1 cut(s) 941
Bse1I ACTGG 4 cut(s) 476, 1057, 1661, 1667
Bse3DI GCAATG 1 cut(s) 951
BseBI CCWGG 3 cut(s) 669, 1032, 1121
BseDI CCNNGG 7 cut(s) 162, 668, 835, 1030, 1119, 1540, 1668
BseGI GGATG 2 cut(s) 711, 1269
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMI GCAATG 1 cut(s) 951
BseMII CTCAG 3 cut(s) 983, 1382, 1671
BseNI ACTGG 4 cut(s) 476, 1057, 1661, 1667
BseXI GCAGC 8 cut(s) 421, 500, 688, 1087, 1123, 1136, 1175, 1208
Bsh1236I CGCG 2 cut(s) 164, 1295
BshFI GGCC 5 cut(s) 170, 261, 465, 950, 1056
BshTI ACCGGT 1 cut(s) 941
BsiHKAI GWGCWC 2 cut(s) 486, 796
BsiSI CCGG 1 cut(s) 942
BslFI GGGAC 2 cut(s) 1161, 1667
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 2 cut(s) 334, 757
BsmFI GGGAC 2 cut(s) 1161, 1667
BsmI GAATGC 1 cut(s) 1347
BsnI GGCC 5 cut(s) 170, 261, 465, 950, 1056
Bsp1286I GDGCHC 3 cut(s) 249, 486, 796
Bsp143I GATC 3 cut(s) 1018, 1042, 1627
Bsp1720I GCTNAGC 1 cut(s) 1219
Bsp19I CCATGG 2 cut(s) 1540, 1668
BspACI CCGC 6 cut(s) 24, 162, 164, 167, 227, 947
BspANI GGCC 5 cut(s) 170, 261, 465, 950, 1056
BspCNI CTCAG 3 cut(s) 982, 1381, 1672
BspFNI CGCG 2 cut(s) 164, 1295
BspHI TCATGA 1 cut(s) 1578
BspLI GGNNCC 4 cut(s) 225, 248, 906, 1149
BspMAI CTGCAG 1 cut(s) 802
BsrDI GCAATG 1 cut(s) 951
BsrFI RCCGGY 1 cut(s) 941
BsrI ACTGG 4 cut(s) 476, 1057, 1661, 1667
BssAI RCCGGY 1 cut(s) 941
BssECI CCNNGG 7 cut(s) 162, 668, 835, 1030, 1119, 1540, 1668
BssMI GATC 3 cut(s) 1018, 1042, 1627
BssT1I CCWWGG 2 cut(s) 1540, 1668
Bst2UI CCWGG 3 cut(s) 669, 1032, 1121
Bst4CI ACNGT 4 cut(s) 889, 993, 1279, 1594
BstAPI GCANNNNNTGC 2 cut(s) 431, 1515
BstBAI YACGTR 1 cut(s) 180
BstC8I GCNNGC 5 cut(s) 88, 486, 1181, 1194, 1209
BstDEI CTNAG 5 cut(s) 779, 969, 1219, 1368, 1680
BstDSI CCRYGG 4 cut(s) 162, 835, 1540, 1668
BstF5I GGATG 2 cut(s) 711, 1269
BstFNI CGCG 2 cut(s) 164, 1295
BstHHI GCGC 1 cut(s) 1590
BstKTI GATC 3 cut(s) 1021, 1045, 1630
BstMAI GTCTC 2 cut(s) 334, 757
BstMBI GATC 3 cut(s) 1018, 1042, 1627
BstMWI GCNNNNNNNGC 8 cut(s) 96, 422, 431, 953, 1133, 1189, 1436, 1515
BstNI CCWGG 3 cut(s) 669, 1032, 1121
BstSCI CCNGG 3 cut(s) 667, 1030, 1119
BstSFI CTRYAG 3 cut(s) 588, 798, 1500
BstUI CGCG 2 cut(s) 164, 1295
BstV1I GCAGC 8 cut(s) 421, 500, 688, 1087, 1123, 1136, 1175, 1208
BstX2I RGATCY 1 cut(s) 1042
BstYI RGATCY 1 cut(s) 1042
BsuI GTATCC 1 cut(s) 841
BsuRI GGCC 5 cut(s) 170, 261, 465, 950, 1056
BtgI CCRYGG 4 cut(s) 162, 835, 1540, 1668
BtsCI GGATG 2 cut(s) 711, 1269
BtsI GCAGTG 2 cut(s) 498, 1368
BtsIMutI CAGTG 4 cut(s) 483, 498, 1368, 1599
Cac8I GCNNGC 5 cut(s) 88, 486, 1181, 1194, 1209
CaiI CAGNNNCTG 2 cut(s) 431, 1063
CciI TCATGA 1 cut(s) 1578
CfoI GCGC 1 cut(s) 1590
Cfr10I RCCGGY 1 cut(s) 941
Cfr13I GGNCC 5 cut(s) 223, 473, 904, 1148, 1665
Cfr42I CCGCGG 1 cut(s) 165
CseI GACGC 2 cut(s) 206, 1284
Csp6I GTAC 4 cut(s) 389, 603, 923, 1283
CspAI ACCGGT 1 cut(s) 941
CviAII CATG 9 cut(s) 51, 274, 724, 820, 959, 1466, 1541, 1579, 1669
CviQI GTAC 4 cut(s) 389, 603, 923, 1283
DdeI CTNAG 5 cut(s) 779, 969, 1219, 1368, 1680
DpnI GATC 3 cut(s) 1020, 1044, 1629
DpnII GATC 3 cut(s) 1018, 1042, 1627
DraI TTTAAA 1 cut(s) 361
DraIII CACNNNGTG 1 cut(s) 791
DrdI GACNNNNNNGTC 1 cut(s) 781
DseDI GACNNNNNNGTC 1 cut(s) 781
EaeI YGGCCR 4 cut(s) 168, 463, 948, 1054
Ecl136II GAGCTC 1 cut(s) 484
Eco130I CCWWGG 2 cut(s) 1540, 1668
Eco147I AGGCCT 1 cut(s) 261
Eco24I GRGCYC 2 cut(s) 249, 486
Eco32I GATATC 1 cut(s) 1318
Eco47I GGWCC 5 cut(s) 223, 473, 904, 1148, 1665
Eco53kI GAGCTC 1 cut(s) 484
Eco57I CTGAAG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 484
EcoRII CCWGG 3 cut(s) 667, 1030, 1119
EcoRV GATATC 1 cut(s) 1318
EcoT14I CCWWGG 2 cut(s) 1540, 1668
EcoT38I GRGCYC 2 cut(s) 249, 486
ErhI CCWWGG 2 cut(s) 1540, 1668
FaeI CATG 9 cut(s) 54, 277, 727, 823, 962, 1469, 1544, 1582, 1672
FaqI GGGAC 2 cut(s) 1161, 1667
FatI CATG 9 cut(s) 50, 273, 723, 819, 958, 1465, 1540, 1578, 1668
FauI CCCGC 1 cut(s) 234
FauNDI CATATG 1 cut(s) 930
FokI GGATG 2 cut(s) 698, 1256
FriOI GRGCYC 2 cut(s) 249, 486
FspBI CTAG 4 cut(s) 60, 374, 413, 1250
FspI TGCGCA 1 cut(s) 1589
GlaI GCGC 1 cut(s) 1589
HaeIII GGCC 5 cut(s) 170, 261, 465, 950, 1056
HapII CCGG 1 cut(s) 942
HgaI GACGC 2 cut(s) 206, 1284
HhaI GCGC 1 cut(s) 1590
Hin1II CATG 9 cut(s) 54, 277, 727, 823, 962, 1469, 1544, 1582, 1672
Hin6I GCGC 1 cut(s) 1588
HinP1I GCGC 1 cut(s) 1588
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HindIII AAGCTT 3 cut(s) 129, 206, 216
HinfI GANTC 2 cut(s) 734, 1366
HpaII CCGG 1 cut(s) 942
HphI GGTGA 6 cut(s) 13, 132, 138, 151, 1099, 1108
Hpy166II GTNNAC 2 cut(s) 649, 892
Hpy188I TCNGA 4 cut(s) 234, 1065, 1423, 1681
Hpy188III TCNNGA 5 cut(s) 212, 337, 1299, 1579, 1631
Hpy8I GTNNAC 2 cut(s) 649, 892
HpyAV CCTTC 2 cut(s) 89, 251
HpyCH4III ACNGT 4 cut(s) 889, 993, 1279, 1594
HpyCH4IV ACGT 1 cut(s) 179
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 422, 431, 953, 1133, 1189, 1436, 1515
HpyF3I CTNAG 5 cut(s) 779, 969, 1219, 1368, 1680
HpySE526I ACGT 1 cut(s) 179
Hsp92II CATG 9 cut(s) 54, 277, 727, 823, 962, 1469, 1544, 1582, 1672
HspAI GCGC 1 cut(s) 1588
KspI CCGCGG 1 cut(s) 165
Kzo9I GATC 3 cut(s) 1018, 1042, 1627
LmnI GCTCC 6 cut(s) 68, 252, 481, 799, 1415, 1672
Lsp1109I GCAGC 8 cut(s) 421, 500, 688, 1087, 1123, 1136, 1175, 1208
LweI GCATC 4 cut(s) 309, 1044, 1136, 1427
MaeI CTAG 4 cut(s) 60, 374, 413, 1250
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 5 cut(s) 157, 750, 783, 1114, 1363
MalI GATC 3 cut(s) 1020, 1044, 1629
MboI GATC 3 cut(s) 1018, 1042, 1627
MboII GAAGA 6 cut(s) 32, 590, 593, 635, 797, 1567
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 1 cut(s) 1042
MhlI GDGCHC 3 cut(s) 249, 486, 796
MlsI TGGCCA 2 cut(s) 465, 1056
MluNI TGGCCA 2 cut(s) 465, 1056
MlyI GAGTC 1 cut(s) 1360
MmeI TCCRAC 1 cut(s) 1579
MnlI CCTC 8 cut(s) 27, 43, 148, 247, 335, 574, 1210, 1675
Mox20I TGGCCA 2 cut(s) 465, 1056
MscI TGGCCA 2 cut(s) 465, 1056
MseI TTAA 8 cut(s) 360, 599, 843, 914, 977, 1004, 1377, 1568
Msp20I TGGCCA 2 cut(s) 465, 1056
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 1 cut(s) 942
MspR9I CCNGG 3 cut(s) 669, 1032, 1121
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 1347
MvaI CCWGG 3 cut(s) 669, 1032, 1121
MvnI CGCG 2 cut(s) 164, 1295
MwoI GCNNNNNNNGC 8 cut(s) 96, 422, 431, 953, 1133, 1189, 1436, 1515
NcoI CCATGG 2 cut(s) 1540, 1668
NdeI CATATG 1 cut(s) 930
NdeII GATC 3 cut(s) 1018, 1042, 1627
NlaIII CATG 9 cut(s) 54, 277, 727, 823, 962, 1469, 1544, 1582, 1672
NlaIV GGNNCC 4 cut(s) 225, 248, 906, 1149
NmuCI GTSAC 4 cut(s) 157, 783, 1114, 1363
NsbI TGCGCA 1 cut(s) 1589
PagI TCATGA 1 cut(s) 1578
PceI AGGCCT 1 cut(s) 261
PctI GAATGC 1 cut(s) 1347
PfeI GAWTC 1 cut(s) 734
PinAI ACCGGT 1 cut(s) 941
PleI GAGTC 1 cut(s) 1360
PpsI GAGTC 1 cut(s) 1360
Ppu21I YACGTR 1 cut(s) 180
PshBI ATTAAT 1 cut(s) 914
PsiI TTATAA 1 cut(s) 1337
Psp124BI GAGCTC 1 cut(s) 486
Psp6I CCWGG 3 cut(s) 667, 1030, 1119
PspGI CCWGG 3 cut(s) 667, 1030, 1119
PspN4I GGNNCC 4 cut(s) 225, 248, 906, 1149
PspPI GGNCC 5 cut(s) 223, 473, 904, 1148, 1665
PsrI GAACNNNNNNTAC 2 cut(s) 1626, 1658
PstI CTGCAG 1 cut(s) 802
PstNI CAGNNNCTG 2 cut(s) 431, 1063
PsuI RGATCY 1 cut(s) 1042
RsaI GTAC 4 cut(s) 390, 604, 924, 1284
RsaNI GTAC 4 cut(s) 389, 603, 923, 1283
SacI GAGCTC 1 cut(s) 486
SacII CCGCGG 1 cut(s) 165
SaqAI TTAA 8 cut(s) 360, 599, 843, 914, 977, 1004, 1377, 1568
Sau3AI GATC 3 cut(s) 1018, 1042, 1627
Sau96I GGNCC 5 cut(s) 223, 473, 904, 1148, 1665
ScaI AGTACT 1 cut(s) 604
SchI GAGTC 1 cut(s) 1360
ScrFI CCNGG 3 cut(s) 669, 1032, 1121
SduI GDGCHC 3 cut(s) 249, 486, 796
SfaNI GCATC 4 cut(s) 309, 1044, 1136, 1427
SfcI CTRYAG 3 cut(s) 588, 798, 1500
Sfr303I CCGCGG 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 165
SinI GGWCC 5 cut(s) 223, 473, 904, 1148, 1665
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 6 cut(s) 24, 162, 164, 167, 227, 947
SspI AATATT 1 cut(s) 912
SspMI CTAG 4 cut(s) 60, 374, 413, 1250
SstI GAGCTC 1 cut(s) 486
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 667, 1030, 1119
StyI CCWWGG 2 cut(s) 1540, 1668
TaaI ACNGT 4 cut(s) 889, 993, 1279, 1594
TaiI ACGT 1 cut(s) 182
TaqII GACCGA 1 cut(s) 1165
TatI WGTACW 4 cut(s) 388, 602, 922, 1282
TauI GCSGC 3 cut(s) 167, 170, 950
TfiI GAWTC 1 cut(s) 734
Tru1I TTAA 8 cut(s) 360, 599, 843, 914, 977, 1004, 1377, 1568
Tru9I TTAA 8 cut(s) 360, 599, 843, 914, 977, 1004, 1377, 1568
TscAI CASTG 4 cut(s) 483, 498, 1368, 1599
TseFI GTSAC 4 cut(s) 157, 783, 1114, 1363
TseI GCWGC 8 cut(s) 434, 488, 701, 1100, 1124, 1136, 1163, 1196
Tsp45I GTSAC 4 cut(s) 157, 783, 1114, 1363
TspDTI ATGAA 4 cut(s) 98, 141, 636, 1567
TspGWI ACGGA 4 cut(s) 239, 634, 824, 1341
TspRI CASTG 4 cut(s) 483, 498, 1368, 1599
VpaK11BI GGWCC 5 cut(s) 223, 473, 904, 1148, 1665
VspI ATTAAT 1 cut(s) 914
XapI RAATTY 2 cut(s) 498, 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 1547
XspI CTAG 4 cut(s) 60, 374, 413, 1250
ZrmI AGTACT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.